Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,068,163 A→T 2.0% intergenic (‑146/+146) glcD ← / ← RCAP_rcc02872 glycolate dehydrogenase, subunit GlcD/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,068,1630A→T 2.0% 5.4 ‑1.1 ‑0.2249intergenic (‑146/+146)glcD/RCAP_rcc02872glycolate dehydrogenase, subunit GlcD/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (173/71):  total (178/71)
Fisher's exact test strand distribution p-value = 3.25e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.52e-01

Only 100 of 319 total aligned reads displayed.

ATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGC  >  NC_014034/3068063‑3068263
                                                                                                     |                                                                                                     
aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCa                                                                                                       <  2:343688/101‑1 (MQ=255)
aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCa                                                                                                       >  2:1074062/1‑101 (MQ=255)
     cgcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGcgcc                                                                                                  >  2:2691120/1‑101 (MQ=255)
      gcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGcgccc                                                                                                 <  2:869260/101‑1 (MQ=255)
        aaCCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGcgcccgc                                                                                               >  1:7133369/1‑101 (MQ=255)
        aaCCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGcgcccgc                                                                                               >  2:5634514/1‑101 (MQ=255)
          ccGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCgcccgccc                                                                                             >  2:2016364/1‑101 (MQ=255)
           cGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGcccgcccc                                                                                            <  2:4598066/101‑1 (MQ=255)
            gAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGcccgccccg                                                                                           >  1:5476262/1‑101 (MQ=255)
              aaCGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCccgccccgcc                                                                                         >  2:6901088/1‑101 (MQ=255)
               aCGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCcgccccgccc                                                                                        <  2:5875479/101‑1 (MQ=255)
                 gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCAt                                                                                      >  2:6422150/1‑101 (MQ=255)
                 gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCAt                                                                                      <  1:934988/101‑1 (MQ=255)
                 gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCAt                                                                                      >  1:3456734/1‑101 (MQ=255)
                 gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCAt                                                                                      <  1:1204179/101‑1 (MQ=255)
                  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATg                                                                                     >  2:1631865/1‑101 (MQ=255)
                  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATg                                                                                     <  2:4000670/101‑1 (MQ=255)
                    gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATggc                                                                                   <  2:4785860/101‑1 (MQ=255)
                      gCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATggcgg                                                                                 >  1:375218/1‑101 (MQ=255)
                       cAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATggcggg                                                                                >  2:1856060/1‑101 (MQ=255)
                        aGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATggcgggg                                                                               >  1:4985546/1‑100 (MQ=255)
                        aGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATggcgggc                                                                               >  1:5669048/1‑101 (MQ=255)
                          gCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGgcgc                                                                             >  1:2147477/1‑101 (MQ=255)
                          gCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGGGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGgcgc                                                                             <  1:334386/101‑1 (MQ=255)
                           cTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCt                                                                            >  1:6702253/1‑101 (MQ=255)
                            tGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCtt                                                                           <  2:735987/101‑1 (MQ=255)
                             gACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTtc                                                                          <  2:3971667/101‑1 (MQ=255)
                             gACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTtc                                                                          <  1:5982952/101‑1 (MQ=255)
                              aCCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTtct                                                                         <  2:7062348/101‑1 (MQ=255)
                               ccGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGGGCCtctc                                                                        >  2:2639815/1‑101 (MQ=255)
                                cGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTtctcc                                                                       >  1:5576502/1‑100 (MQ=255)
                                 gAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGGGCTTCTcgc                                                                      >  2:5279342/1‑101 (MQ=255)
                                  aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcgcc                                                                     >  2:4252239/1‑101 (MQ=255)
                                  aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcgcc                                                                     >  1:3811616/1‑101 (MQ=255)
                                  aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGCCCAGCCCCCGCCCAGCCCATGGGGGGCGCTTCTcccc                                                                     >  1:1784442/1‑101 (MQ=17)
                                   ggCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcgccc                                                                    <  2:2380221/101‑1 (MQ=255)
                                    gCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcgcccc                                                                   >  1:4181283/1‑100 (MQ=255)
                                      cATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcgcccgcc                                                                 >  1:190081/1‑101 (MQ=255)
                                       agcGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCccgccg                                                                <  1:2612853/99‑1 (MQ=255)
                                       aTCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCcccccg                                                                >  2:5128545/1‑101 (MQ=255)
                                        tCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCgg                                                               <  2:6355028/101‑1 (MQ=255)
                                         cGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGGGCTTCTCGCCCCCCcgg                                                              >  2:2400206/1‑101 (MQ=255)
                                           ccGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGgggc                                                            <  2:5900992/101‑1 (MQ=255)
                                            cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCGCAAA.GGCCCTCGGCGAGCGCCCGCCCCGCCCATGGCGGGCGCCTCTcgcccgcgcgcgcg                                                           >  1:2320545/1‑94 (MQ=17)
                                            cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGgggcg                                                           <  2:6571944/101‑1 (MQ=255)
                                            cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGgggcg                                                           >  1:5908862/1‑101 (MQ=255)
                                            cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCCTCTCGCCCGCCCgggcg                                                           >  1:5180238/1‑101 (MQ=255)
                                            cGAGGTCATGCGGAACAGTGGCCTCGTGACCACCGAGGGCCCAAA.GGCCATCGGACAGCGCCCGCCCCGCCCATGGCGGGGGCATATCGCCCCCCagggag                                                           >  2:3031153/1‑99 (MQ=17)
                                             gAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCCTCTCGCCCGCCGgggcgg                                                          >  2:1919860/1‑101 (MQ=255)
                                             gAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCACTTCTCGCCCGCCCgggcgg                                                          >  1:6851169/1‑101 (MQ=255)
                                              gggTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGgggcggg                                                         <  1:4558195/100‑1 (MQ=255)
                                              aGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGGGCTTCTCGCCCGCCGgggcggg                                                         >  2:5344913/1‑101 (MQ=255)
                                                 tCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCCGGGCGGgcgc                                                      >  2:2186769/1‑101 (MQ=255)
                                                  cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCCCCCGGGCGGGGGcc                                                     >  2:3842292/1‑101 (MQ=255)
                                                    tGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCgg                                                   <  2:5546431/101‑1 (MQ=255)
                                                     gCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCCGc                                                  >  1:2389918/1‑101 (MQ=255)
                                                           cggTGGCCTGGGGCCCGCCGGGGGCCCAAG.GCCCCCCGGCCGGCCCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTctgc                                            <  2:4029572/99‑1 (MQ=11)
                                                           cAGTGGCCTCGGGACCGCCGAGGGCCCAAG.GCCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTctgc                                            <  1:5799025/101‑1 (MQ=255)
                                                           cAGTGCCCTCGTGCCCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTccgc                                            <  1:1378865/101‑1 (MQ=255)
                                                            aGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGGGGCGGCCCccgct                                           >  1:6315245/1‑101 (MQ=14)
                                                             gTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGGGCCCGCCCccgctc                                          >  2:6089498/1‑100 (MQ=14)
                                                              tGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCtgctgc                                         >  2:3379585/1‑101 (MQ=255)
                                                               ggCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGGGCCGGGCCCTGCTgcg                                        >  2:115954/1‑101 (MQ=255)
                                                               ggCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTgcg                                        >  1:269031/1‑101 (MQ=255)
                                                                  cccgggcccgccgGGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCt                                     <  1:6143391/94‑1 (MQ=255)
                                                                  cTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCCCCGCCGCGccc                                     >  1:1492076/1‑100 (MQ=255)
                                                                   tCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCCCTGCTGCGcccg                                    >  1:4909535/1‑99 (MQ=255)
                                                                    cGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCCGGGCGGGCGCCGGCCCCTGCTGCGCCC.gg                                  >  2:3503763/1‑101 (MQ=255)
                                                                    cGTGACCGCCCAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGGGCCGGCCTCTGCTGCGCCC.gg                                  >  2:1503253/1‑101 (MQ=255)
                                                                     agacccgccg.GGGCCCAAA.GGCCCCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCCTCTCCCCCGCCGGGGCCGGCCCCCGCCCCTGCCCCGCCttacg                                 >  1:817200/5‑97 (MQ=11)
                                                                      tGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGGGCCGGCCTCTGCTGCGCCT.gggg                                >  1:6423179/1‑101 (MQ=255)
                                                                      ggcccgccgGGGGCCAAAA.GCCCCTCGGCCGGCCCCCCCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.gggg                                <  1:3688509/98‑1 (MQ=14)
                                                                       gACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGGGCCCGCCCCTGCCGCGCCT.tgggg                               >  2:2210500/1‑100 (MQ=14)
                                                                        cccgccgAGGGCCCAAG.GCCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGaa                              <  2:57783/100‑1 (MQ=255)
                                                                           gccgAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGGGCCGGCCCCCGCTGCGCCC.gggggcacg                           >  2:6825239/1‑96 (MQ=14)
                                                                           gccgAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGGGCCCGCCTCTGCTGCGCCC.GGGGCAACg                           >  2:4344764/1‑101 (MQ=14)
                                                                            ccgAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGGAACgg                          >  2:4443304/1‑101 (MQ=255)
                                                                            ccgAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCCCTGCTGCGCCT.GGGGGAACgg                          >  1:1027589/1‑101 (MQ=255)
                                                                             cgAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGt                         >  1:3105435/1‑101 (MQ=255)
                                                                             cgAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCC.GGGGGAACggg                         >  2:5073501/1‑100 (MQ=255)
                                                                                gggCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCtt                      >  2:3603584/1‑101 (MQ=255)
                                                                                gcccacAAC.GGCCCCCGGCCGGCGCCCGCCCCGCCCCTGGCGGGCGTTTCTCGCCCGCCGGGCCGGGCGCCGGCCTCTGTTGTGCCTCGCGGAAACGGTCt                       <  1:2209889/96‑1 (MQ=11)
                                                                                 ggCCCAAG.GGCCCCCCTCCCCCGCCCGCCCCCCCCCTCGCGCGCCCCTCTCCCCAGCCGGGCCGGCCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTg                     <  1:626369/101‑1 (MQ=9)
                                                                                   cccAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCGATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCCCTGCTGCGCCT.GGGGGAACGGGCTTGGc                   >  1:5432623/1‑101 (MQ=17)
                                                                                    ccAAAGGGCCCCCGGCCGGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGc                   <  2:6144941/101‑1 (MQ=18)
                                                                                    ccAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGGCTTGGCa                  >  1:6994947/1‑101 (MQ=255)
                                                                                     cAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.CGGGAAACGGTCTTGGCAc                 >  1:2514093/1‑101 (MQ=255)
                                                                                     cAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCC.GGGGGAACGGTCTTGGCAc                 >  1:5241203/1‑101 (MQ=255)
                                                                                       aa.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGGAACGGTCTTGGCACCg               >  2:1237175/1‑101 (MQ=255)
                                                                                       aa.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGGAACGGGCTTGGCAccc               >  1:468777/1‑100 (MQ=255)
                                                                                        a.GGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCCCCCCCCCGGCCCGGGGCCGGCCGCGGTCGCGCTC.GGGGGGACGGGCGTGGCCCCgg              >  1:6554013/1‑100 (MQ=2)
                                                                                          ggCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGGAACGGTCTTGGCACCGcc             >  2:1048862/1‑101 (MQ=255)
                                                                                          ggCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCCCTGCTGCGCCC.GGGGAAACGGGCTTGGCACCGcc             >  2:6993727/1‑101 (MQ=255)
                                                                                          ggCCCCCGCCCAGCCCCCGCCCCGCCCAGGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGcc             <  2:6463748/101‑1 (MQ=18)
                                                                                          gccccCCGGCCGGCCCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGcc             <  2:4509273/99‑1 (MQ=255)
                                                                                           gCCCTCGCCCAGCCCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGccc            <  1:2961039/101‑1 (MQ=255)
                                                                                            cccTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGGAACGGTCTTGGCACCGCCCg           >  1:4629744/1‑101 (MQ=255)
                                                                                              cTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCcgg         >  2:2995587/1‑101 (MQ=255)
                                                                                                cGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGGGACGGTCCTGGCACCCCCCCGGcc       >  2:2888433/1‑100 (MQ=17)
                                                                                                 ggCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGGAACGGTCTTGGCACCGCCCGGGCTc      >  1:1586202/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
ATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGC  >  NC_014034/3068063‑3068263

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG