Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,068,179 A→T 2.0% intergenic (‑162/+130) glcD ← / ← RCAP_rcc02872 glycolate dehydrogenase, subunit GlcD/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,068,1790A→T 2.0% 5.1 ‑1.8 ‑0.2255intergenic (‑162/+130)glcD/RCAP_rcc02872glycolate dehydrogenase, subunit GlcD/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (159/90):  total (164/91)
Fisher's exact test strand distribution p-value = 1.64e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.05e-01

Only 100 of 312 total aligned reads displayed.

CGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAG  >  NC_014034/3068079‑3068279
                                                                                                    |                                                                                                       
cGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCa                                                                                                         >  2:6100716/1‑101 (MQ=255)
 gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCAt                                                                                                        <  1:1204179/101‑1 (MQ=255)
 gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCAt                                                                                                        <  2:2489339/101‑1 (MQ=255)
 gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCAt                                                                                                        <  1:934988/101‑1 (MQ=255)
  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATg                                                                                                       >  2:5234158/1‑101 (MQ=255)
  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATg                                                                                                       <  2:566751/101‑1 (MQ=255)
  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAAGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATg                                                                                                       >  1:5105427/1‑101 (MQ=255)
   tGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATgg                                                                                                      <  2:3999737/101‑1 (MQ=255)
    gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggc                                                                                                     <  2:4785860/101‑1 (MQ=255)
       cAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcggg                                                                                                  >  2:1856060/1‑101 (MQ=255)
        aGGCTGGCCGAGGCCATCGCCGAGGTCATGCGAAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcgggc                                                                                                 <  2:165427/101‑1 (MQ=255)
        aGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcgggc                                                                                                 >  2:4661523/1‑101 (MQ=255)
        aGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcgggc                                                                                                 >  1:6284308/1‑101 (MQ=255)
        aGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcgggc                                                                                                 >  1:5669048/1‑101 (MQ=255)
          gCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGgcg.c                                                                                              >  1:5942689/1‑101 (MQ=255)
            tGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCTCCCCGCCCTTGGCGGgcg.cct                                                                                            >  1:7061410/1‑99 (MQ=255)
            tGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.Ct.t                                                                                           <  2:735987/101‑1 (MQ=255)
             gACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.tc                                                                                          <  1:2654940/101‑1 (MQ=255)
              aCCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.tct                                                                                         <  2:6425228/101‑1 (MQ=255)
              aCCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.tct                                                                                         <  2:7062348/101‑1 (MQ=255)
                cGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.tctcc                                                                                       >  1:3099825/1‑100 (MQ=255)
                cGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.tctcc                                                                                       >  1:5576502/1‑100 (MQ=255)
                cGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTcg                                                                                       <  2:1283349/101‑1 (MQ=255)
                  aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTcgcc                                                                                     >  2:4252239/1‑101 (MQ=255)
                  aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTcgcc                                                                                     >  1:3811616/1‑101 (MQ=255)
                   ggCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTcgccc                                                                                    <  2:4505439/101‑1 (MQ=255)
                    gCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTcgcccg                                                                                   <  1:4661762/101‑1 (MQ=255)
                      cATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTcgcccgcc                                                                                 >  1:190081/1‑101 (MQ=255)
                       aTCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCcccccg                                                                                >  2:5128545/1‑101 (MQ=255)
                        tCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCgg                                                                               <  2:6355028/101‑1 (MQ=255)
                           ccGAGGTCATGCGGAACAGTGCCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGgggc                                                                            <  2:6883215/101‑1 (MQ=255)
                            cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCGCAAAGGCCCTCGGCGAGCGCCCGCCCCGCCCATGGCGGGCG.CC.TCTcgcccgcgcgcgcg                                                                           >  1:2320545/1‑94 (MQ=17)
                            cGAGGTCATGCGGAACAGTGGCCTCGTGACCACCGAGGGCCCAAAGGCCATCGGACAGCGCCCGCCCCGCCCATGGCGGGGG.CA.TATCGCCCCCCagggag                                                                           >  2:3031153/1‑99 (MQ=17)
                              gggTCAGGCGGACCAGGGCCCCCGGGCCCGCCGAGGCCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGgggcggg                                                                         <  1:6931261/100‑1 (MQ=11)
                                gTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGTCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGggcgggcg                                                                       >  2:3458825/1‑101 (MQ=255)
                                gTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCCGggcgggcg                                                                       >  2:1507709/1‑101 (MQ=255)
                                 tCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCCGGGCGGgcgc                                                                      >  2:2186769/1‑101 (MQ=255)
                                  cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGCGGCGGGGGcc                                                                     >  2:1910852/1‑101 (MQ=255)
                                  cATGCGGAACAGTGGCCTCCTCACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGGGcc                                                                     <  1:6831288/101‑1 (MQ=255)
                                   aTGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGGGCCg                                                                    >  2:887945/1‑101 (MQ=255)
                                   aTGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCg                                                                    <  2:5896562/101‑1 (MQ=255)
                                     gCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGGGCCCGc                                                                  >  1:2594728/1‑101 (MQ=255)
                                     gCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGc                                                                  <  2:2978865/101‑1 (MQ=255)
                                       ggAACAGGGCCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCct                                                                <  1:2392341/101‑1 (MQ=255)
                                        gaccggTGGCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCctc                                                               <  2:2345595/96‑1 (MQ=255)
                                        gAACAGTGGCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCctc                                                               <  2:6563668/101‑1 (MQ=255)
                                        gAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CC.TCTCGCCCGCCGGGGCGGGCGCCGGcccc                                                               >  2:6169153/1‑99 (MQ=255)
                                           cggTGGCCTGGGGCCCGCCGGGGGCCCAAGGCCCCCCGGCCGGCCCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTctgc                                                            <  2:4029572/99‑1 (MQ=11)
                                           cAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCCCCCGCCGGGGCGGGCGCCGGCCTctgc                                                            >  2:4327636/1‑101 (MQ=255)
                                           cAGTGGCCTCGGGACCGCCGAGGGCCCAAGGCCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTctgc                                                            <  1:5799025/101‑1 (MQ=255)
                                           cAGTGCCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTccgc                                                            <  1:1378865/101‑1 (MQ=255)
                                            ggTGCCCTCGTGCCCGCCGGGGGCCCAAGGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTctgct                                                           <  1:4216600/100‑1 (MQ=14)
                                            aatGGCCCCGTGACCGGCGAGGGCCCAAAGGCCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGGGCCG.TGGCGCCCCCCGGGGGGGGGGGCGGccccccg                                                            >  1:1781749/3‑96 (MQ=17)
                                            aatGGCCCCGTGACCGGCGAGGGCCCAAAGGCCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGGG.CTGTGGCGCCCCCCGGGGGGGGGGGCgggccccgg                                                            >  2:106394/3‑94 (MQ=17)
                                               ggCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTgcg                                                        >  1:269031/1‑101 (MQ=255)
                                               ggCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCCGGGCGGGCGCCGGCCCCCGCTccg                                                        >  1:1491831/1‑101 (MQ=14)
                                               ggCCCCGGGACCGCCGGGGCGCCAAAGCGTCCCGGCCAGCGCCCGCCCCGCCCCTGGCGGGCG.GT.TATCGCCCGCCGGGCCGGGCGCCGGGCCccgctggc                                                        >  1:618426/1‑99 (MQ=11)
                                                  ctggTGACCGCGGAGGGCCCAAGGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCt                                                     <  2:3010011/98‑1 (MQ=255)
                                                   tCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCCCTGCTGCGcccg                                                    >  1:4909535/1‑99 (MQ=255)
                                                   ccGTGCCCGCGGAGGGCCAAAGGGCCCTCGCCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.g                                                   <  2:5834901/100‑1 (MQ=14)
                                                   caagacccgccg.GGGCCCAAAGGCCCCCGGCCAGCGCCCGCCCCGCCCATGGCGGGGG.CT.TCTCGCCCGCCGGGCCGGGCGCCGGCCTCCGGCCCGCCT.gg                                                  >  1:1552921/7‑101 (MQ=17)
                                                     gTGACCGGCGAGGGCCCAAAGGCCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGCGCCG.TCGCGCCCCCCGGGGGGGGCGCCAGCCCCCGGTTCGCCttg                                                   >  2:3833861/1‑99 (MQ=11)
                                                     agacccgccg.GGGCCCAAAGGCCCCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CC.TCTCCCCCGCCGGGGCCGGCCCCCGCCCCTGCCCCGCCttacg                                                 >  1:817200/5‑97 (MQ=11)
                                                      tGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGGGCCGGCCTCTGCTGCGCCT.gggg                                                >  1:6423179/1‑101 (MQ=255)
                                                      ggcccgccgAGGGCCCAACGCCCCTCGCCCGGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.gggg                                                <  2:5531164/98‑1 (MQ=14)
                                                      ggcccgccgAGGGCCAAAAGGCCCCCGCCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.gggg                                                <  2:2389824/98‑1 (MQ=14)
                                                      ggACCGCGGGGGGCCAAAGGCCCCTCGGCGAGCCCCCGCCCCACCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.gggg                                                <  2:2645734/100‑1 (MQ=14)
                                                       gcccgccgGGGGCCCAAGGCCCCCCGGCCGGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGa                                               <  1:377608/99‑1 (MQ=14)
                                                       gACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGGGCCCGCCCCTGCCGCGCCT.tgggg                                               >  2:2210500/1‑100 (MQ=14)
                                                        cccgccgGGGGCCCAAGGGCCCCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGaa                                              <  2:4358011/100‑1 (MQ=255)
                                                         ccgccgAGGGCCAAAGGGCCCCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGaaa                                             <  1:6655321/101‑1 (MQ=255)
                                                           gccgGGGGCCCAAAGGCCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGGGCCG.TCGCGGCGCCCGGGCAGGGGGCCGGGCGCCGCTGTGCCT.cgggggag                                            >  1:4575431/1‑97 (MQ=2)
                                                            ccgAGTGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGGCGGCCTCTGCTGCGCCC.GGGGGAACgg                                          >  2:3519777/1‑101 (MQ=14)
                                                            ccgAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCCCTGCTGCGCCT.GGGGGAACgg                                          >  1:1027589/1‑101 (MQ=255)
                                                             cgggggCCCAAAGGCCGCCGGCCAACGCCCGCCCCGCCCATGGCGGGGG.CG.TCTCCCCCCCCCCGCCGGGGGGCGGGCCCGGGTGCGCCT.CGGGGGACggg                                         >  2:3395695/4‑100 (MQ=2)
                                                             cgAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCC.GGGGGAACggg                                         >  2:5073501/1‑100 (MQ=255)
                                                              gAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACgggc                                        >  2:3969481/1‑99 (MQ=255)
                                                                gcccacAACGGCCCCCGGCCGGCGCCCGCCCCGCCCCTGGCGGGCG.TT.TCTCGCCCGCCGGGCCGGGCGCCGGCCTCTGTTGTGCCTCGCGGAAACGGTCt                                       <  1:2209889/96‑1 (MQ=11)
                                                                   cccAACGCCCCTCCGCCGGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCCCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGc                                   <  1:3276684/101‑1 (MQ=18)
                                                                   cccAAAGGCCCTCGGCCAGCGCCCGCCCCGCCGATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCCCTGCTGCGCCT.GGGGGAACGGGCTTGGc                                   >  1:5432623/1‑101 (MQ=17)
                                                                   cccAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGc                                   >  1:1925563/1‑101 (MQ=255)
                                                                     cAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.CGGGAAACGGTCTTGGCAc                                 >  1:2514093/1‑101 (MQ=255)
                                                                       aggccccTCGGCCGGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCg                               <  1:6101028/97‑1 (MQ=255)
                                                                        aGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGGCTTGGCACCGc                              >  1:5202334/1‑101 (MQ=255)
                                                                         ggCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGGAACGGTCTTGGCACCGcc                             >  2:1048862/1‑101 (MQ=255)
                                                                         ggCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCCCTGCTGCGCCC.GGGGAAACGGGCTTGGCACCGcc                             >  2:6993727/1‑101 (MQ=255)
                                                                          gCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCGCTGCTGCGCCT.GGGGAAACGGGCTTGGCCCCGccc                            >  1:3373211/1‑101 (MQ=255)
                                                                          ccccGCGGCCAGCGCCCGCCCCGCCCAGGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGccc                            <  2:1194391/100‑1 (MQ=255)
                                                                               cGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGGCTTGGCACCGCCCGGGcc                       >  1:3716902/1‑100 (MQ=255)
                                                                                 gcgggcgcCCGCCCCGCCCAGGGCGTGCG.CT.TCTCGCCCGCCGGGGGGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTcc                     <  2:5576212/97‑1 (MQ=18)
                                                                                  ccggccccccccccgcCCAGGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCcg                    <  2:2838223/95‑1 (MQ=18)
                                                                                  ccAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCcg                    >  1:1808540/1‑101 (MQ=255)
                                                                                   cAGCCCCCGCCCCGCCCAGGGCGGGCG.CT.TCTCGCCCGCGGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCcgg                   <  2:2009243/101‑1 (MQ=255)
                                                                                          cgccccgcccATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCGgcgggcgg            >  1:5367307/1‑101 (MQ=255)
                                                                                               cgcccATGGCGGGCG.CT.TCCCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGaaa       <  1:3503899/101‑1 (MQ=255)
                                                                                               cgcccAGGGGGGGCG.CT.TCTCGCCCGCGGGGGCGGGGGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGaaa       <  1:612951/101‑1 (MQ=25)
                                                                                               cgcccAGGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGaaa       <  2:6701911/101‑1 (MQ=255)
                                                                                                  ccATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGGAAGGc    >  2:5310600/1‑101 (MQ=255)
                                                                                                  ccATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGGAAGGc    >  1:5468547/1‑101 (MQ=255)
                                                                                                    aTGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAg  >  1:2883482/1‑101 (MQ=255)
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CGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CT.TCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAG  >  NC_014034/3068079‑3068279

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG