Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,068,180 T→C 2.0% intergenic (‑163/+129) glcD ← / ← RCAP_rcc02872 glycolate dehydrogenase, subunit GlcD/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,068,1800T→C 2.0% 2.6 ‑1.8 ‑0.8251intergenic (‑163/+129)glcD/RCAP_rcc02872glycolate dehydrogenase, subunit GlcD/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (158/85):  total (163/88)
Fisher's exact test strand distribution p-value = 1.68e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.72e-01

Only 100 of 312 total aligned reads displayed.

GATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGT  >  NC_014034/3068080‑3068280
                                                                                                     |                                                                                                      
gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCAt                                                                                                        <  1:1204179/101‑1 (MQ=255)
 aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATg                                                                                                       >  2:5234158/1‑101 (MQ=255)
 aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATg                                                                                                       >  2:1631865/1‑101 (MQ=255)
 aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATg                                                                                                       <  2:566751/101‑1 (MQ=255)
 aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATg                                                                                                       >  1:979724/1‑101 (MQ=255)
  tGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATgg                                                                                                      <  2:3999737/101‑1 (MQ=255)
   gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATggc                                                                                                     >  1:5384933/1‑101 (MQ=255)
   gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATggc                                                                                                     <  2:4785860/101‑1 (MQ=255)
   gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATggc                                                                                                     >  1:5693396/1‑101 (MQ=255)
       aGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATggcgggc                                                                                                 >  2:4661523/1‑101 (MQ=255)
           tGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGgcg.cct                                                                                            >  2:1051680/1‑99 (MQ=255)
           tGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.Ctt                                                                                            <  2:735987/101‑1 (MQ=255)
             aCCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTtct                                                                                          <  2:7062348/101‑1 (MQ=255)
             aCCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTtct                                                                                          <  2:6425228/101‑1 (MQ=255)
              ccGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTtctc                                                                                         <  1:3389793/101‑1 (MQ=255)
              ccGAGGCCATCCCCGAGGTCATGCGGAACAGTGGCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTCGGCC.GGCGCCCGCCCCGCCCATGGCGGGCG.CTtctc                                                                                         <  1:2379142/101‑1 (MQ=255)
                 aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGGG.CTTCTcgcc                                                                                      >  2:3016034/1‑101 (MQ=255)
                 aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTcgcc                                                                                      >  2:4252239/1‑101 (MQ=255)
                  ggCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTcgccc                                                                                     <  2:4505439/101‑1 (MQ=255)
                     cATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTcgcccgcc                                                                                  >  1:190081/1‑101 (MQ=255)
                      ctCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCccgccg                                                                                 <  2:3097564/100‑1 (MQ=255)
                      agcGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCccgccg                                                                                 <  1:2612853/99‑1 (MQ=255)
                      aTCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCcccccg                                                                                 >  2:5128545/1‑101 (MQ=255)
                       tCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCgg                                                                                <  2:6355028/101‑1 (MQ=255)
                        cGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCggg                                                                               >  1:6686075/1‑101 (MQ=255)
                          ccGAGGTCATGCGGAACAGTGCCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGgggc                                                                             <  2:6883215/101‑1 (MQ=255)
                           cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGgggcg                                                                            >  1:5908862/1‑101 (MQ=255)
                            gAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CCTCTCGCCCGCCGgggcgg                                                                           >  2:1919860/1‑101 (MQ=255)
                            gAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCA.CTTCTCGCCCGCCCgggcgg                                                                           >  1:6851169/1‑101 (MQ=255)
                               gTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGTCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGggcgggcg                                                                        >  2:3458825/1‑101 (MQ=255)
                                 cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGGGcc                                                                      >  1:4916222/1‑101 (MQ=255)
                                  aTGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGGGCCg                                                                     >  2:887945/1‑101 (MQ=255)
                                  aTGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCg                                                                     <  2:5896562/101‑1 (MQ=255)
                                       gAACAGTGGCCTCGTGCCCGCCGAGGGCCCAAGGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCctc                                                                <  1:4324104/101‑1 (MQ=255)
                                       gAACAGTGGCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCctc                                                                <  2:6563668/101‑1 (MQ=255)
                                       gAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCctc                                                                <  2:2199214/101‑1 (MQ=255)
                                       gAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CCTCTCGCCCGCCGGGGCGGGCGCCGGcccc                                                                >  2:6169153/1‑99 (MQ=255)
                                        aaCGGTGCCCCCGGGCCCCCCGGGGCCCCAAGGCCCCTCGCCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCctct                                                               <  1:727314/101‑1 (MQ=1)
                                        aaCAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGGG.CTTCTCGCCCGCCGGGGCGGGCGCCgggccct                                                               >  1:6464078/1‑96 (MQ=255)
                                        aaCAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATAGCGGGCG.CCTCTCCGCCGCCGCGGCGGGCGCCGGccccc                                                               >  2:2399378/1‑98 (MQ=17)
                                          cggTGGCCTGGGGCCCGCCGGGGGCCCAAGGCCCCCCGGCC.GGCCCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTctgc                                                             <  2:4029572/99‑1 (MQ=11)
                                          cAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTctgc                                                             >  1:6103633/1‑101 (MQ=255)
                                          cAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCCCCCGCCGGGGCGGGCGCCGGCCTctgc                                                             >  2:4327636/1‑101 (MQ=255)
                                          cAGTGCCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTccgc                                                             <  1:1378865/101‑1 (MQ=255)
                                           aatGGCCCCGTGACCGGCGAGGGCCCAAAGGCCCTCGGCC.TGCGCCCGCCCCGCCATCGGCGGGGGCCGTGGCGCCCCCCGGGGGGGGGGGCGGccccccg                                                             >  1:1781749/3‑96 (MQ=17)
                                           aGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGGGGCGGCCCccgct                                                            >  1:6315245/1‑101 (MQ=14)
                                            gTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGGGCCCGCCCccgctc                                                           >  2:6089498/1‑100 (MQ=14)
                                              ggCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCCGGGCGGGCGCCGGCCCCCGCTccg                                                         >  1:1491831/1‑101 (MQ=14)
                                               gCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCCGCCCCTGCTgcgc                                                        >  1:5460333/1‑101 (MQ=255)
                                                 cccgggcccgccgGGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCt                                                      <  1:6143391/94‑1 (MQ=255)
                                                  ccGTGCCCGCGGAGGGCCAAAGGGCCCTCGCCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.g                                                    <  2:5834901/100‑1 (MQ=14)
                                                  caagacccgccg.GGGCCCAAAGGCCCCCGGCC.AGCGCCCGCCCCGCCCATGGCGGGGG.CTTCTCGCCCGCCGGGCCGGGCGCCGGCCTCCGGCCCGCCT.gg                                                   >  1:1552921/7‑101 (MQ=17)
                                                   cGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCCGCGCCT.gg                                                   >  1:2402633/1‑101 (MQ=255)
                                                    gTGACCGGCGAGGGCCCAAAGGCCCTCGGCC.TGCGCCCGCCCCGCCATCGGCGGGCGCCGTCGCGCCCCCCGGGGGGGGCGCCAGCCCCCGGTTCGCCttg                                                    >  2:3833861/1‑99 (MQ=11)
                                                    gTGACCACCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGGGCCGGCCTCCGCCGCGCCC.ggg                                                  >  1:845538/1‑101 (MQ=14)
                                                     ggcccgccgAGGGCCCAACGCCCCTCGCCC.GGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.gggg                                                 <  2:5531164/98‑1 (MQ=14)
                                                      gACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCCGCTGCGCCC.GGGGa                                                >  1:6973481/1‑101 (MQ=255)
                                                       cccgccgGGGGCCCAAGGGCCCCCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGaa                                               <  2:4358011/100‑1 (MQ=255)
                                                          gccgAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACg                                            >  1:3723417/1‑101 (MQ=255)
                                                           ccgAGTGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGGCGGCCTCTGCTGCGCCC.GGGGGAACgg                                           >  2:3519777/1‑101 (MQ=14)
                                                           ccgAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGGAACgg                                           >  1:4125485/1‑101 (MQ=255)
                                                           ccgAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCCCTGCTGCGCCT.GGGGGAACgg                                           >  1:1027589/1‑101 (MQ=255)
                                                            cgggggCCCAAAGGCCGCCGGCC.AACGCCCGCCCCGCCCATGGCGGGGG.CGTCTCCCCCCCCCCGCCGGGGGGCGGGCCCGGGTGCGCCT.CGGGGGACggg                                          >  2:3395695/4‑100 (MQ=2)
                                                            cgAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGt                                          >  1:3105435/1‑101 (MQ=255)
                                                            cgAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCC.GGGGGAACggg                                          >  2:5073501/1‑100 (MQ=255)
                                                            cgAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCCGCCCCCGCTGCGCCT.GGGGAAACggg                                          >  2:1329601/1‑100 (MQ=255)
                                                             gAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGGAACgggc                                         >  1:4603346/1‑99 (MQ=255)
                                                             gAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACgggc                                         >  2:3969481/1‑99 (MQ=255)
                                                               gcccacAACGGCCCCCGGCC.GGCGCCCGCCCCGCCCCTGGCGGGCG.TTTCTCGCCCGCCGGGCCGGGCGCCGGCCTCTGTTGTGCCTCGCGGAAACGGTCt                                        <  1:2209889/96‑1 (MQ=11)
                                                                gccccAAGGGCCCTCGGCC.GGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTg                                      <  1:5238787/99‑1 (MQ=255)
                                                                  cccAAGGCCCCCCGCCC.GGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGCTCTTGGc                                    <  2:2594632/101‑1 (MQ=17)
                                                                  cccAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGc                                    >  1:1925563/1‑101 (MQ=255)
                                                                    cAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGGCTTGGCAc                                  >  2:2357918/1‑101 (MQ=255)
                                                                     cacggCCCCCGGCCGGGCCCCCGCCCCGCCCCTGGCGGGCG.CTTCTCGCCCGCCGGGCGGGGCGCCGGCCTCTGTTGTGCCTCGCGGAAACGGTCCTgcac                                   <  2:2052040/98‑4 (MQ=11)
                                                                      aaGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGGAACGGGCTTGGCAccc                                >  1:468777/1‑100 (MQ=255)
                                                                       aGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGGCCCTGCTGCGCCT.GGGGAAACGGGCTTGGCACCGc                               >  1:5159385/1‑101 (MQ=255)
                                                                       aGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCCTGGCACCGc                               >  2:1896524/1‑101 (MQ=255)
                                                                        ggCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCCCTGCTGCGCCC.GGGGAAACGGGCTTGGCACCGcc                              >  2:6993727/1‑101 (MQ=255)
                                                                         gCCCTCGCCC.AGCCCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGccc                             <  1:2961039/101‑1 (MQ=255)
                                                                          cccCCGGCC.AGCGCCCGCCCCGCCCAGGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCg                            <  1:560781/101‑1 (MQ=255)
                                                                           ccTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGGAACGGGCTTGGCACCGccccg                           >  2:2988016/1‑99 (MQ=255)
                                                                            ccccgcccggcgcCCGCCCCGCCCGTGGCGGGCG.CTTCTCGCCCGCCGGGGGGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCgg                           <  1:2548961/92‑1 (MQ=17)
                                                                                gCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGGAACGGTCTTGGCACCGCCCGGGCTcc                      >  2:1116030/1‑101 (MQ=255)
                                                                                gCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCCGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCCGGCTcc                      >  2:2673835/1‑101 (MQ=255)
                                                                                 gcgggcgcCCGCCCCGCCCAGGGCGTGCG.CTTCTCGCCCGCCGGGGGGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTcc                      <  2:5576212/97‑1 (MQ=18)
                                                                                 cc.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCcg                     >  1:1808540/1‑101 (MQ=255)
                                                                                  ccggcgcCCGCCCCGCCCATGGGGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCcg                     <  1:5513856/98‑1 (MQ=255)
                                                                                      cgcccgccCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCCGGCTCcggcgg                 >  1:3303185/1‑101 (MQ=255)
                                                                                       gcccgcccCGCCCGGGGCGGGCG.CTTCTCGCCCGCGGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCggcggg                <  2:1749750/101‑1 (MQ=255)
                                                                                       gcccgcccCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCCCCCGGGCTCCggccgg                >  1:6227555/1‑101 (MQ=255)
                                                                                        cccgccccgcCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCggcgggc               >  1:5975307/1‑101 (MQ=255)
                                                                                         ccgccccgccCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCggcgggcg              >  1:2723125/1‑101 (MQ=255)
                                                                                           gccccgcccATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGcgggcggg            >  1:3609675/1‑101 (MQ=255)
                                                                                            ccccgcccATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGGCTCTGCTGCGCCT.GGGGAAACGGGCTTGGCACCGCCCGGGCTCCGGCGGGCgggg           >  2:2888014/1‑101 (MQ=255)
                                                                                             cccgcccATGGCGGGCG.CTTCTGGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGa          <  2:2302187/101‑1 (MQ=255)
                                                                                             cccgcccATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGa          >  1:378817/1‑101 (MQ=255)
                                                                                             cccgcccATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGa          >  1:2656226/1‑101 (MQ=255)
                                                                                               cgcccAGGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGaaa        <  2:6701911/101‑1 (MQ=255)
                                                                                                  ccATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGGAAGGc     >  2:5310600/1‑101 (MQ=255)
                                                                                                  ccATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGGAAGGc     >  2:1221274/1‑101 (MQ=255)
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GATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCC.AGCGCCCGCCCCGCCCATGGCGGGCG.CTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGT  >  NC_014034/3068080‑3068280

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG