Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,068,222 C→T 2.0% intergenic (‑205/+87) glcD ← / ← RCAP_rcc02872 glycolate dehydrogenase, subunit GlcD/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,068,2220C→T 2.0% 4.3 ‑0.4 ‑0.2254intergenic (‑205/+87)glcD/RCAP_rcc02872glycolate dehydrogenase, subunit GlcD/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (90/159):  total (91/163)
Fisher's exact test strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.99e-01

Only 100 of 339 total aligned reads displayed.

CAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCAGGATCG  >  NC_014034/3068122‑3068322
                                                                                                    |                                                                                                    
cAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCCCCCGCCGGGGCGGGCGCCGGCCTctgc                                                                                                      >  2:4327636/1‑101 (MQ=255)
 ggTGCCCTCGTGCCCGCCGGGGGCCCAAGGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTctgct                                                                                                     <  1:4216600/100‑1 (MQ=14)
    ggCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCGCCCGGGGCGGGCGCCGGCCTctgctggg                                                                                                  >  1:6179248/1‑99 (MQ=255)
    ggCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGGGCCGGGCCCTGCTgcg                                                                                                  >  2:115954/1‑101 (MQ=255)
    ggCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTgcg                                                                                                  >  1:269031/1‑101 (MQ=255)
    ggCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCCCCGCTgcg                                                                                                  >  1:1502863/1‑101 (MQ=255)
       cTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGCGGCCGGCCCCTGCTGCGccc                                                                                               >  1:5621943/1‑100 (MQ=255)
         cGTGCCCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTgg                                                                                             <  2:813702/101‑1 (MQ=255)
         cGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCCGCGCCTgg                                                                                             >  1:2402633/1‑101 (MQ=255)
          gTGACCACCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGGGCCGGCCTCCGCCGCGCCCggg                                                                                            >  1:845538/1‑101 (MQ=14)
          agacccgccg.GGGCCCAAAGGCCCCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCCTCTCCCCCGCCGGGGCCGGCCCCCGCCCCTGCCCCGCCttacg                                                                                           >  1:817200/5‑97 (MQ=11)
           tgcccgccgAGGGCCCAAGGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTgggg                                                                                           <  1:3794961/98‑1 (MQ=255)
           tGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGGGCCGGCCTCTGCTGCGCCTgggg                                                                                           >  1:6423179/1‑101 (MQ=255)
           ggcccgccgGGGGCCAAAAGCCCCTCGGCCGGCCCCCCCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTgggg                                                                                           <  1:3688509/98‑1 (MQ=14)
            gACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCCggggg                                                                                          >  2:4247087/1‑100 (MQ=255)
            gACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCCGGGCGGGCGCCGGCCTCTGCTGCGCCTggggg                                                                                          >  2:6286538/1‑100 (MQ=255)
              cggccgGGGGCCAAAGGGCCCTCGGCCAGCGCCCCCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGaaa                                                                                        <  2:5863165/99‑1 (MQ=14)
                gcggAGGCCCCAAAGCCCCCCGCCCGGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACg                                                                                      <  2:5551900/98‑1 (MQ=17)
                 ccgAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGGAACgg                                                                                     >  2:4443304/1‑101 (MQ=255)
                 ccgAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCCCTGCTGCGCCTGGGGGAACgg                                                                                     >  1:1027589/1‑101 (MQ=255)
                  cgggggCCCAAAGGCCGCCGGCCAACGCCCGCCCCGCCCATGGCGGGGGCGTCTCCCCCCCCCCGCCGGGGGGCGGGCCCGGGTGCGCCTCGGGGGACggg                                                                                    >  2:3395695/4‑100 (MQ=2)
                  cgAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCCGGGGGAACggg                                                                                    >  2:5073501/1‑100 (MQ=255)
                  cgAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCCGCCCCCGCTGCGCCTGGGGAAACggg                                                                                    >  2:1329601/1‑100 (MQ=255)
                   gAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGGAACgggc                                                                                   >  1:4603346/1‑99 (MQ=255)
                     gggCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCtt                                                                                 >  2:3603584/1‑101 (MQ=255)
                      gccccAAGGGCCCTCGGCCGGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTg                                                                                <  1:5238787/99‑1 (MQ=255)
                         ccAAGGCCCCTCTCCCAGCGCCCGCCCCCCCCTTGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCa                                                                             <  2:915171/101‑1 (MQ=17)
                         ccAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGGCTTGGCa                                                                             >  1:6994947/1‑101 (MQ=255)
                         ccAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGGCTTGGCa                                                                             >  2:407736/1‑101 (MQ=255)
                          cAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGGCTTGGCAc                                                                            >  2:2357918/1‑101 (MQ=255)
                          cAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCCCCGGGGCGGGCGGCGGGCTCTGCCGCGCCCTGGGGAACGGGCTTGGCAc                                                                            >  2:7001898/1‑101 (MQ=14)
                           aaaGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGGAACGGTCTTGGCAcc                                                                           >  2:6797776/1‑101 (MQ=255)
                            agggCCCTCGGCGAGCCCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCg                                                                          <  2:4013462/99‑1 (MQ=255)
                            aggccccTCGGCCGGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCg                                                                          <  1:6101028/97‑1 (MQ=255)
                            aaGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGGAACGGGCCTGGCAccc                                                                          >  2:5290320/1‑100 (MQ=255)
                             aGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGGCCCTGCTGCGCCTGGGGAAACGGGCTTGGCACCGc                                                                         >  1:5159385/1‑101 (MQ=255)
                             aGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCCTGGCACCGc                                                                         >  2:1896524/1‑101 (MQ=255)
                                 ccccgcccggcgcCCGCCCCGCCCGTGGCGGGCGCTTCTCGCCCGCCGGGGGGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCgg                                                                     <  1:2548961/92‑1 (MQ=17)
                                    cGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGcc                                                                  >  1:137592/1‑100 (MQ=255)
                                     ggCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGGAACGGTCTTGGCACCGCCCGGGCTc                                                                 >  1:1586202/1‑101 (MQ=255)
                                     ggCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTc                                                                 >  1:5397197/1‑101 (MQ=255)
                                      gcaaGCGCCCGCCCCGCCCGGGGCGGGCGCTTCTCGCCCGCTGGGGGGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTcc                                                                <  2:6973108/98‑1 (MQ=18)
                                      gCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCCGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCCGGCTcc                                                                >  2:2673835/1‑101 (MQ=255)
                                      cccggcccccgccccgcCCAGGGCGGGCGCTTCGCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTcc                                                                <  1:148843/94‑1 (MQ=18)
                                       ccgcgccccgccccgcCCATGGCGGGCGCTCCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCcg                                                               <  1:1856132/95‑1 (MQ=255)
                                            gcccgcccCGCCCGGGGCGGGCGCTTCTCGCCCGCGGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCggcggg                                                          <  2:1749750/101‑1 (MQ=255)
                                                  cccgcccATGGCGGGCGCTTCTGGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGa                                                    <  2:2302187/101‑1 (MQ=255)
                                                    cgcccAGGGGGGGCGCTTCTCGCCCGCGGGGGCGGGGGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGaaa                                                  <  1:612951/101‑1 (MQ=25)
                                                    cgcccAGGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGaaa                                                  <  2:6701911/101‑1 (MQ=255)
                                                       cgagggcggccgCTCCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGc                                               <  2:4657791/97‑1 (MQ=255)
                                                       ccATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGGAAGGc                                               >  1:5468547/1‑101 (MQ=255)
                                                       ccATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGc                                               >  1:5915855/1‑101 (MQ=255)
                                                       ccAGGGCGGGCGCTTCTCCCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGc                                               <  1:1919932/101‑1 (MQ=255)
                                                          gggggggcgCTTCTCCCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGt                                            <  2:5194652/97‑1 (MQ=255)
                                                           ggcgggcgCTTCTCGCCCGCCGGGCCGGGCGTCGGCCTGTGTCGCGCTTGGGGAAACGGCCTTGCCCCGGGGCGTGCACAGGCGGGCggggggcgccggcc                                           >  1:6943147/1‑91 (MQ=21)
                                                           ggcgggcgCTTCTCGCCCCCCGGGCCGGGCGTCGGCCCGTGTCGCGCTTGGGGGAACGGGCTTGCCACGGGCCTTGCACAGGCGGGGGGGGGAAgcgggcg                                           >  2:7099483/1‑95 (MQ=21)
                                                            gggggcgCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGc                                          <  1:339754/99‑1 (MQ=255)
                                                            gggggcgCTTCTCCCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGc                                          <  2:435170/99‑1 (MQ=255)
                                                            gcgggcgCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGc                                          <  1:3512631/101‑1 (MQ=255)
                                                            gcgggcgCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGc                                          >  2:4390777/1‑101 (MQ=255)
                                                             gggccgcTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGcc                                         <  2:6343597/97‑1 (MQ=255)
                                                               gtggttccgcgccccccGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCtt                                       <  2:6177826/92‑1 (MQ=37)
                                                                gcgcTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCttt                                      >  1:1632256/1‑101 (MQ=255)
                                                                gcgcTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCttt                                      <  2:3821305/101‑1 (MQ=255)
                                                                   cTTCTCGCCCGCCGGGGCGGCCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCt                                   <  1:6286861/101‑1 (MQ=255)
                                                                    ttCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCCGGCTCCGGCGGGCGCGGCAAAGCAGGGCCTGTCCtc                                  >  2:3324555/1‑100 (MQ=21)
                                                                        cgccggccGGGGCGGCCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATcc                              <  2:2835373/101‑1 (MQ=255)
                                                                         gcccgcgGGGGCGGGCCCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCg                             <  2:2622295/101‑1 (MQ=255)
                                                                         gcccgccGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCg                             >  2:5393070/1‑101 (MQ=255)
                                                                            cgccgGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGccc                          <  2:5915552/101‑1 (MQ=255)
                                                                               cgGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTTCTGATCCGCCCGaa                       >  2:3051268/1‑101 (MQ=255)
                                                                               cgGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGaa                       >  2:2928833/1‑101 (MQ=255)
                                                                               cgGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGaa                       <  1:3157593/101‑1 (MQ=255)
                                                                               cgGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGaa                       >  2:2088204/1‑101 (MQ=255)
                                                                                ggggCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGaaa                      <  2:1112519/101‑1 (MQ=255)
                                                                                ggggCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGaaa                      <  2:1492016/101‑1 (MQ=255)
                                                                                ggggCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGaaa                      >  2:1917097/1‑101 (MQ=255)
                                                                                ggggCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGaaa                      >  2:6443233/1‑101 (MQ=255)
                                                                                  ggcggtctCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATc                    <  2:5654060/101‑1 (MQ=255)
                                                                                  ggcgggcgCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGGCTTTCCTGATCCGCCCGAAAta                    >  1:4475467/1‑100 (MQ=255)
                                                                                  ggcgggcgCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATc                    <  1:5073761/101‑1 (MQ=255)
                                                                                  ggcgggcgCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATc                    <  1:1221322/101‑1 (MQ=255)
                                                                                   gcggcccCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATcc                   <  2:5621653/101‑1 (MQ=255)
                                                                                     gggcgCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATcccc                 <  2:2878275/101‑1 (MQ=255)
                                                                                      ggcgCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATccccc                >  2:5788968/1‑101 (MQ=255)
                                                                                       gcgcCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCt               <  2:102170/101‑1 (MQ=255)
                                                                                       gcgcCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCt               >  1:3877342/1‑101 (MQ=255)
                                                                                         cccGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCa             <  2:5134827/100‑1 (MQ=255)
                                                                                           cGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAgc           <  2:6103321/101‑1 (MQ=255)
                                                                                           cGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAgc           >  1:2035754/1‑101 (MQ=255)
                                                                                           cGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAgc           <  1:4824221/101‑1 (MQ=255)
                                                                                            ggCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAgcg          <  1:3770591/101‑1 (MQ=255)
                                                                                             gCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAgcgc         <  1:1153167/101‑1 (MQ=255)
                                                                                                tctGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCAgg      <  1:3273224/101‑1 (MQ=255)
                                                                                                tctGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCAgg      <  1:5962924/101‑1 (MQ=255)
                                                                                                  tgctgcGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCAGGAt    <  2:4878500/101‑1 (MQ=255)
                                                                                                  tgctgcGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCAGGAt    <  1:276439/101‑1 (MQ=255)
                                                                                                  tgctgcGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCAGGAt    <  1:2995696/101‑1 (MQ=255)
                                                                                                   gctgcGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCAGGATc   <  2:5468275/101‑1 (MQ=255)
                                                                                                    ctgcGCCTGGGGAAACGGGCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCAGGATcg  <  1:6703179/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
CAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGTGCCTTTCCTGATCCGCCCGAAATCCCCCTCAGCGCAGGATCG  >  NC_014034/3068122‑3068322

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG