Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 317,927 A→G 100% K338E (AAG→GAG)  RCAP_rcc00280 → hemolysin‑type calcium‑binding repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034317,9270A→G100.0% 1463.8 386K338E (AAG→GAG) RCAP_rcc00280hemolysin‑type calcium‑binding repeat family protein
Reads supporting (aligned to +/- strand):  new base (201/185):  ref base (0/0):  total (201/185)

Only 100 of 394 total aligned reads displayed.

AAGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCAAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATCCGC  >  NC_014034/317829‑318024
                                                                                                  |                                                                                                 
aaGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAg                                                                                                 >  2:6702741/1‑101 (MQ=255)
aaGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAg                                                                                                 <  2:5364297/101‑1 (MQ=255)
aaGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAg                                                                                                 >  2:2202974/1‑101 (MQ=255)
 aGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAgg                                                                                                <  1:1014267/101‑1 (MQ=255)
  gTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGa                                                                                               >  1:538199/1‑101 (MQ=255)
    gACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGAtg                                                                                             <  2:165282/101‑1 (MQ=255)
    gACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGAtg                                                                                             >  1:3685064/1‑101 (MQ=255)
       gCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGc                                                                                          >  2:5553006/1‑101 (MQ=255)
       gCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGc                                                                                          <  2:2892089/101‑1 (MQ=255)
        cTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGct                                                                                         >  1:3234144/1‑101 (MQ=255)
         tGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGctc                                                                                        >  1:2692296/1‑101 (MQ=255)
          ggAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGctct                                                                                       >  2:885961/1‑101 (MQ=255)
            aGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTg                                                                                     <  2:4579932/101‑1 (MQ=255)
               ccGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATc                                                                                  <  1:1178161/101‑1 (MQ=255)
               ccGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATc                                                                                  <  2:4879276/101‑1 (MQ=255)
               ccGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATc                                                                                  >  2:2540878/1‑101 (MQ=255)
                cgcgACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCa                                                                                 >  2:1718990/1‑101 (MQ=255)
                   gacgacCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGc                                                                              >  1:3131498/1‑101 (MQ=255)
                      gacCGAGGGCAATGTCTTGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCg                                                                           <  2:2440409/101‑1 (MQ=255)
                      gacCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCg                                                                           >  1:1400880/1‑101 (MQ=255)
                      gacCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCg                                                                           >  1:6424799/1‑101 (MQ=255)
                        ccGGGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAg                                                                         <  1:6698662/101‑1 (MQ=255)
                         cGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAgg                                                                        >  1:436231/1‑101 (MQ=255)
                          gAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGa                                                                       >  2:4027092/1‑101 (MQ=255)
                           aGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGaa                                                                      >  2:1416970/1‑101 (MQ=255)
                           aGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGaa                                                                      >  1:3979067/1‑101 (MQ=255)
                              gCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTc                                                                   <  1:3789565/101‑1 (MQ=255)
                                 aTGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGgc                                                                <  2:760068/101‑1 (MQ=255)
                                   gTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGgcgc                                                              >  1:2483498/1‑101 (MQ=255)
                                   gTCTGGATCACGCTGAACGGGGGGCATCTGGCCGAGGAGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGgcgc                                                              >  1:7071542/1‑101 (MQ=255)
                                    tCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGcc                                                             <  2:2352444/101‑1 (MQ=255)
                                     cTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCa                                                            <  2:2678684/101‑1 (MQ=255)
                                       ggATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATc                                                          <  2:6392247/101‑1 (MQ=255)
                                       ggATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATc                                                          >  2:6124388/1‑101 (MQ=255)
                                        gATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCt                                                         <  1:5983101/101‑1 (MQ=255)
                                         aTCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTg                                                        <  2:4712910/101‑1 (MQ=255)
                                          tCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGa                                                       >  1:3453757/1‑101 (MQ=255)
                                          tCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGa                                                       >  2:6815299/1‑101 (MQ=255)
                                            aCGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGAcc                                                     <  2:629278/101‑1 (MQ=255)
                                            aCGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGAcc                                                     >  2:1469305/1‑101 (MQ=255)
                                            aCGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGAcc                                                     <  1:2135890/101‑1 (MQ=255)
                                            aCGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGAcc                                                     >  2:4939618/1‑101 (MQ=255)
                                            aCGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGAcc                                                     <  1:4076706/101‑1 (MQ=255)
                                             cGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCg                                                    >  2:4153624/1‑101 (MQ=255)
                                               cTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCggg                                                  >  2:3232290/1‑101 (MQ=255)
                                                tGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGc                                                 >  2:2976844/1‑101 (MQ=255)
                                                tGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGc                                                 >  1:147225/1‑101 (MQ=255)
                                                 gAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGcc                                                >  2:4428847/1‑101 (MQ=255)
                                                 gAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGcc                                                <  1:4642625/101‑1 (MQ=255)
                                                  aaCGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCt                                               <  1:1964134/101‑1 (MQ=255)
                                                  aaCGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCt                                               >  1:552319/1‑101 (MQ=255)
                                                   aCGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTg                                              <  2:5298597/101‑1 (MQ=255)
                                                   aCGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTg                                              <  2:605254/101‑1 (MQ=255)
                                                   aCGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTg                                              <  2:2108090/101‑1 (MQ=255)
                                                    cGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTgg                                             >  1:930001/1‑101 (MQ=255)
                                                    cGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCACATGCCCGGGCCtcg                                             >  2:2305514/1‑99 (MQ=255)
                                                     ggggTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTggg                                            >  2:313939/1‑101 (MQ=255)
                                                     ggggTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTggg                                            >  1:4777169/1‑101 (MQ=255)
                                                     ggggTGCATCTGGCCGAGGTGGTCCATGCGGGCGATCTGCGCGCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTggg                                            >  2:3399163/1‑101 (MQ=255)
                                                      gggTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTgggg                                           >  2:5112214/1‑101 (MQ=255)
                                                        gTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCt                                         <  1:1787690/101‑1 (MQ=255)
                                                         tGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCtt                                        >  1:3063504/1‑101 (MQ=255)
                                                          gCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTc                                       >  1:1530653/1‑101 (MQ=255)
                                                           cATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTcc                                      >  1:1291980/1‑101 (MQ=255)
                                                           cATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTcc                                      <  1:2908509/101‑1 (MQ=255)
                                                            aTCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTccc                                     <  1:355273/101‑1 (MQ=255)
                                                            aTCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTccc                                     >  2:5895297/1‑101 (MQ=255)
                                                            aTCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTccc                                     >  1:6315033/1‑101 (MQ=255)
                                                            aTCTGGCCGAGGTGGTCGATGCGGGCGATCTACGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGCCCGGGCCTGGGGCTTccc                                     >  2:1203539/1‑101 (MQ=255)
                                                             tCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCt                                    <  2:3566430/101‑1 (MQ=255)
                                                             tCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCt                                    >  1:718935/1‑101 (MQ=255)
                                                              cTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCtt                                   >  2:3839642/1‑101 (MQ=255)
                                                               tGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCttt                                  <  1:885985/101‑1 (MQ=255)
                                                                  ccGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGcc                               >  1:5839871/1‑101 (MQ=255)
                                                                    gAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGcccc                             >  1:5259296/1‑101 (MQ=255)
                                                                    gAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGcccc                             <  2:127114/101‑1 (MQ=255)
                                                                    gAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCGTCCCTTTGcccc                             <  1:5081918/101‑1 (MQ=255)
                                                                     aGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCg                            <  1:6236735/101‑1 (MQ=255)
                                                                         ggtCGATGCGGGCGATCTGCGCCCCGAGGATGTCCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCttt                        >  1:5020485/1‑101 (MQ=255)
                                                                          gtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCtttt                       <  1:4731943/101‑1 (MQ=255)
                                                                          gtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCtttt                       <  2:7005128/101‑1 (MQ=255)
                                                                           tCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTc                      <  1:7011012/101‑1 (MQ=255)
                                                                            cGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTcc                     >  2:6208857/1‑101 (MQ=255)
                                                                             gATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCg                    <  2:6598648/101‑1 (MQ=255)
                                                                             gATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCg                    >  1:6096670/1‑101 (MQ=255)
                                                                             gATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCg                    >  2:1862257/1‑101 (MQ=255)
                                                                                gCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGAACGGGCGTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTa                 >  1:2323662/1‑101 (MQ=255)
                                                                                 cGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTaa                >  2:4351066/1‑101 (MQ=255)
                                                                                  gggCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTaaa               <  1:1656284/101‑1 (MQ=255)
                                                                                   ggCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAc              >  1:1864/1‑101 (MQ=255)
                                                                                     cGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgc            >  2:6918117/1‑101 (MQ=255)
                                                                                      gATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgcc           <  2:5012870/101‑1 (MQ=255)
                                                                                       aTCGGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCGCGCTTTTCCGCTAAAcgccg          >  1:5594601/1‑101 (MQ=255)
                                                                                         cTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGGGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgccggc        >  1:3887445/1‑99 (MQ=255)
                                                                                         cTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACgccgcc        <  1:6522748/101‑1 (MQ=255)
                                                                                          tGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCa       >  2:182481/1‑101 (MQ=255)
                                                                                          tGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCa       >  1:2541293/1‑101 (MQ=255)
                                                                                            cgcCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATc     >  2:6511457/1‑101 (MQ=255)
                                                                                            cgcCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATc     <  2:2849833/101‑1 (MQ=255)
                                                                                             gcCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATcc    <  1:4422475/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
AAGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCAAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATCCGC  >  NC_014034/317829‑318024

Base quality scores:  ATCG  < 3 ≤  ATCG  < 25 ≤  ATCG  < 32 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG