Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,212,679 T→G 100% intergenic (‑1300/+58) RCAP_rcc03018 ← / ← dctM3 hypothetical protein/TRAP C4‑dicarboxylate transport system permease subunit DctM

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,212,6790T→G100.0% 753.6 211intergenic (‑1300/+58)RCAP_rcc03018/dctM3hypothetical protein/TRAP C4‑dicarboxylate transport system permease subunit DctM
Reads supporting (aligned to +/- strand):  new base (108/103):  ref base (0/0):  total (108/103)

Only 100 of 215 total aligned reads displayed.

GCTCATGGGGATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCATGGGGGGCCCAAGGCCCGGAACGTCGCATGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGCCAGACTGATCGCC  >  NC_014034/3212631‑3212776
                                                |                                                                                                 
gctggccatgATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTgcgc                                               <  1:2151301/92‑1 (MQ=18)
  tggccatgATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGGACGCCGCAGGGCCGGGGAAGCCCGGCCCCGcgcgg                                             >  1:6494620/8‑100 (MQ=17)
  tggccatgATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGCACGGCGCAGGGCCGGGAAACCCCGGCCCTGCCCGa                                             >  1:3316949/8‑101 (MQ=18)
  tggccatgATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGa                                             >  1:3634415/8‑101 (MQ=21)
  tggccatgATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGa                                             >  1:3432331/8‑101 (MQ=21)
  tggccatgATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGa                                             >  1:7079786/8‑101 (MQ=21)
  tggccatgATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGa                                             >  2:399291/8‑101 (MQ=21)
    gccatgATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGaaa                                           <  1:1007395/96‑1 (MQ=21)
    gccatgATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGaaa                                           >  1:6622467/6‑101 (MQ=255)
     ccatgATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAg                                          >  1:468644/5‑101 (MQ=255)
      catgATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGt                                         <  2:2006219/98‑1 (MQ=255)
      catgATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGt                                         >  1:826777/4‑101 (MQ=255)
       atgATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGGCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCGGCGCGACAggt                                        >  2:3323412/3‑99 (MQ=21)
       atgATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGtt                                        <  1:1268448/99‑1 (MQ=255)
       atgATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGtt                                        <  1:6045081/99‑1 (MQ=255)
       atgATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGtt                                        >  1:6452720/3‑101 (MQ=255)
       atgATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGtt                                        <  1:6817262/99‑1 (MQ=255)
        tgaacccccTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACGCCGGCCCTGCGCGAAAGTTa                                       >  1:5605194/5‑101 (MQ=21)
        tgATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTa                                       <  2:5448440/100‑1 (MQ=255)
        tgATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTa                                       >  2:5772464/2‑101 (MQ=255)
        tgATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTa                                       <  1:429752/100‑1 (MQ=255)
        tgATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTa                                       >  2:3415046/2‑101 (MQ=255)
         gaccccccTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTAc                                      <  2:6331926/98‑1 (MQ=255)
         gATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGGAAGGTAc                                      >  2:520015/1‑101 (MQ=21)
         gATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTAc                                      >  2:6321188/1‑101 (MQ=255)
         gATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTAc                                      <  1:2516550/101‑1 (MQ=255)
          aTCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACa                                     <  2:6032731/101‑1 (MQ=255)
          aTCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACa                                     >  1:963230/1‑101 (MQ=255)
            cccccTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATg                                   >  2:3631213/1‑101 (MQ=255)
            cccccTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATg                                   >  2:4593265/1‑101 (MQ=255)
             ccccTCGTGGTCCGTGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGc                                  <  1:2289599/101‑1 (MQ=255)
             ccccTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGc                                  >  1:3884606/1‑101 (MQ=255)
              cccTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGcc                                 <  1:3485084/101‑1 (MQ=255)
              cccTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGcc                                 <  2:805219/101‑1 (MQ=255)
               ccTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCa                                <  1:1625891/101‑1 (MQ=255)
               ccTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCa                                <  1:1467595/101‑1 (MQ=255)
               ccTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCa                                <  2:3906136/101‑1 (MQ=255)
                cTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCaa                               <  2:3172027/101‑1 (MQ=255)
                cTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCaa                               >  1:4064042/1‑101 (MQ=255)
                cTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGCAAACCCCGGCCCTGCGCGAAAGTTACATGCCaa                               <  1:5026282/101‑1 (MQ=255)
                cTCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGAAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCaa                               >  2:2732511/1‑101 (MQ=255)
                 tCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAg                              >  2:2074984/1‑101 (MQ=255)
                 tCGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAg                              <  1:3999114/101‑1 (MQ=255)
                  cGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGc                             >  1:3343523/1‑101 (MQ=255)
                  cGTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGc                             <  1:1891692/101‑1 (MQ=255)
                   gTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCa                            <  1:902108/101‑1 (MQ=255)
                   gTGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCa                            <  1:1659056/101‑1 (MQ=255)
                    tGGTCGGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAg                           >  1:4240041/1‑101 (MQ=255)
                    tGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAg                           >  2:1325516/1‑101 (MQ=255)
                    tGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAg                           >  1:285886/1‑101 (MQ=255)
                    tGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAg                           >  2:2945376/1‑101 (MQ=255)
                    tGGTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCAAAGGCCCGGAACGGCGAAGGGCCGGGAAACCACGGCGCCGCGCGAAAGTTACATGCCAAGCAg                           >  1:2314275/1‑101 (MQ=25)
                     ggTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCGCGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAgc                          >  2:875440/1‑100 (MQ=37)
                     ggTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGa                          >  1:2353680/1‑101 (MQ=255)
                     ggTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGa                          <  1:594783/101‑1 (MQ=255)
                      gTCCGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGAt                         <  1:4979597/101‑1 (MQ=255)
                        ccGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTg                       <  2:4938148/101‑1 (MQ=255)
                        ccGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTg                       <  1:2712401/101‑1 (MQ=255)
                        ccGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTg                       >  2:5523676/1‑101 (MQ=255)
                        ccGGGGCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTg                       <  2:2510546/101‑1 (MQ=255)
                          ggggCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTGCATGCCAAGCAGATTggg                     >  1:5259323/1‑101 (MQ=255)
                          ggggCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTggg                     >  2:1042618/1‑101 (MQ=255)
                          ggggCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCCGCGCGAAAGTTACATGCCAAGCCGATTggg                     >  2:6574521/1‑101 (MQ=37)
                           gggCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGc                    <  1:3101541/101‑1 (MQ=255)
                           gggCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGc                    >  2:1679308/1‑101 (MQ=255)
                            ggCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCa                   >  1:5683114/1‑101 (MQ=255)
                            ggCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCa                   >  2:5096790/1‑101 (MQ=255)
                            ggCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCa                   <  2:4047929/101‑1 (MQ=255)
                             gCAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCaa                  <  2:5353289/101‑1 (MQ=255)
                              cAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAg                 >  1:6943325/1‑101 (MQ=255)
                              cAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAg                 <  2:4749694/101‑1 (MQ=255)
                              cAGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAg                 >  2:4624034/1‑101 (MQ=255)
                               aGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGCAAGTTACATGCCAAGCAGATTGGGCAAgg                >  2:1804242/1‑101 (MQ=38)
                               aGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAgg                >  2:5902238/1‑101 (MQ=255)
                               aGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAgg                <  1:5404417/101‑1 (MQ=255)
                               aGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAgg                >  1:3807619/1‑101 (MQ=255)
                               aGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAgg                <  1:5187126/101‑1 (MQ=255)
                               aGGATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAgg                >  1:3031311/1‑101 (MQ=255)
                                ggATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGc               >  2:6734550/1‑101 (MQ=255)
                                 gATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGcc              >  2:6145329/1‑101 (MQ=255)
                                 gATAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAAAGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAACTTACATGCCAAGCAGATTGGGCAAgaca              >  2:3525409/1‑98 (MQ=38)
                                  gtAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGCCa             <  1:5322336/100‑1 (MQ=255)
                                   tAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGCCAg            <  1:5887854/101‑1 (MQ=255)
                                   tAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGCCAg            <  2:7003069/101‑1 (MQ=255)
                                   tAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGCCAg            <  1:5826068/101‑1 (MQ=255)
                                   tAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGCCAg            >  2:1221286/1‑101 (MQ=255)
                                   tAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGCCAg            <  2:1572449/101‑1 (MQ=255)
                                   tAGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGACGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGCCAg            >  2:2456253/1‑101 (MQ=255)
                                    aGGGGCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGCCAGa           <  2:92865/101‑1 (MQ=255)
                                     ggggCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGCCAGAc          >  2:643985/1‑101 (MQ=255)
                                      gggCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGCCAGACt         <  1:632870/101‑1 (MQ=255)
                                      gggCGGCGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGCCAGACt         <  2:6813705/101‑1 (MQ=255)
                                       ggcggcGCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGCCAGACTg        >  2:5223273/1‑101 (MQ=255)
                                          ggcgCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGCCAGACTGATc     >  1:2040066/1‑101 (MQ=255)
                                          ggcgCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGCCAGACTGATc     <  2:4783772/101‑1 (MQ=255)
                                          ggcgCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGCCAGACTGATc     <  1:6741100/101‑1 (MQ=255)
                                          ggcgCAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGCCAGACTGATc     <  2:2330071/101‑1 (MQ=255)
                                            cgcAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGCCAGatcggaaga   >  2:2985757/1‑93 (MQ=38)
                                            cgcAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGCCAGACTGATCGc   >  2:5147412/1‑101 (MQ=255)
                                            cgcAGGGGGGGCCCAAGGCCCGGAACGTCGCAGGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGCCAGACTGATCGc   <  2:21792/101‑1 (MQ=255)
                                                |                                                                                                 
GCTCATGGGGATCCCCCTCGTGGTCCGGGGCAGGATAGGGGCGGCGCATGGGGGGCCCAAGGCCCGGAACGTCGCATGGCCGGGAAACCCCGGCCCTGCGCGAAAGTTACATGCCAAGCAGATTGGGCAAGGCCAGACTGATCGCC  >  NC_014034/3212631‑3212776

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG