Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,216,136 C→A 100% A65D (GCT→GAT)  dctS3 → C4‑dicarboxylate transport sensor protein DctS

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,216,1360C→A100.0% 1239.1 313A65D (GCT→GAT) dctS3C4‑dicarboxylate transport sensor protein DctS
Reads supporting (aligned to +/- strand):  new base (137/175):  ref base (0/0):  total (137/176)

Only 100 of 331 total aligned reads displayed.

GCTGGTGGCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGCTGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTCGCAA  >  NC_014034/3216038‑3216233
                                                                                                  |                                                                                                 
gtgggggccGATCGTCGCGCTGATGCCGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCActgatg                                                                                                 <  1:6942574/93‑1 (MQ=255)
gCTGGTGGCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCActgatg                                                                                                 >  2:5482393/1‑101 (MQ=255)
gCTGGTGGCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCActgatg                                                                                                 <  2:3926072/101‑1 (MQ=255)
gCTGGTGGCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCActgatg                                                                                                 >  1:6416256/1‑101 (MQ=255)
 cTGGTGGCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACtgatgc                                                                                                <  1:5232045/101‑1 (MQ=255)
 cTGGTGGCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACtgatgc                                                                                                <  1:5547156/101‑1 (MQ=255)
  tggtggCGATCGTCGCGCTGATGGCGCTGGTTGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAGCCGCGCCCGGGCGAAACTGGCCACTGATgcg                                                                                               <  1:4555651/101‑1 (MQ=255)
    gtggCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCt                                                                                             <  2:4516110/101‑1 (MQ=255)
     tggCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCtt                                                                                            <  1:5828014/101‑1 (MQ=255)
      ggCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCttt                                                                                           <  2:432690/101‑1 (MQ=255)
       gCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTg                                                                                          >  1:2506570/1‑101 (MQ=255)
       gCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTg                                                                                          >  2:2677996/1‑101 (MQ=255)
           tcgtcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTc                                                                                      >  2:1746764/1‑101 (MQ=255)
            cgtcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCg                                                                                     <  2:1209699/101‑1 (MQ=255)
            cgtcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCg                                                                                     <  1:1397778/101‑1 (MQ=255)
             gtcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGa                                                                                    >  1:1182917/1‑101 (MQ=255)
             gtcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGa                                                                                    <  1:4111041/101‑1 (MQ=255)
             gtcgCGCTGATGACGCTGGTCGGCGCGCTGCTCGTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGa                                                                                    >  1:5004756/1‑101 (MQ=255)
              tcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGaa                                                                                   >  1:3672088/1‑101 (MQ=255)
               cgcgCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAAc                                                                                  <  1:4369587/101‑1 (MQ=255)
                gcgcTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACa                                                                                 <  2:3585555/101‑1 (MQ=255)
                 cgcTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAg                                                                                <  2:3352836/101‑1 (MQ=255)
                  gcTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGa                                                                               <  2:1169048/101‑1 (MQ=255)
                   cTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGAc                                                                              <  1:4858016/101‑1 (MQ=255)
                   cTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGAc                                                                              <  1:10281/101‑1 (MQ=255)
                   cTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGGCGAACAgcc                                                                              >  2:2318429/1‑99 (MQ=255)
                     gatgaCGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGc                                                                            >  1:2730867/1‑101 (MQ=255)
                       tgaCGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTg                                                                          >  2:6180678/1‑101 (MQ=255)
                        gaCGCTGGTCGTCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTgc                                                                         <  2:6473148/101‑1 (MQ=255)
                        gaCGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTgc                                                                         <  1:1750791/101‑1 (MQ=255)
                         aCGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTgcg                                                                        <  1:1814033/101‑1 (MQ=255)
                            cTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCtt                                                                     >  2:88131/1‑101 (MQ=255)
                             tGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCttt                                                                    >  1:1260690/1‑101 (MQ=255)
                              ggTCGGCGCGCTGCTCTTCGCCGTCGCGGAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTc                                                                   >  1:6276927/1‑101 (MQ=255)
                              ggTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTc                                                                   <  2:6302177/101‑1 (MQ=255)
                              ggTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTc                                                                   >  1:5379586/1‑101 (MQ=255)
                                tCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAg                                                                 <  1:4458746/101‑1 (MQ=255)
                                  ggCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGAt                                                               <  1:2660522/101‑1 (MQ=255)
                                  ggCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGAt                                                               <  1:7014681/101‑1 (MQ=255)
                                   gcgcgcTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATg                                                              >  1:4898975/1‑101 (MQ=255)
                                    cgcgcTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATgg                                                             <  2:3758515/101‑1 (MQ=255)
                                    cgcgcTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATgg                                                             >  1:5749823/1‑101 (MQ=255)
                                     gcgcTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGc                                                            <  1:2845422/101‑1 (MQ=255)
                                      cgcTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGca                                                           <  1:6169314/101‑2 (MQ=255)
                                       gctgctCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCgg                                                          >  1:875508/1‑101 (MQ=255)
                                       gctgctCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCgg                                                          >  1:6067979/1‑101 (MQ=255)
                                       gctgctCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCgg                                                          <  2:5189898/101‑1 (MQ=255)
                                       gctgctCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCgg                                                          >  2:4847443/1‑101 (MQ=255)
                                        ctgctCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGt                                                         >  1:2085497/1‑101 (MQ=255)
                                        ctgctCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGt                                                         >  2:4744792/1‑101 (MQ=255)
                                         tgctCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTg                                                        >  1:6212737/1‑101 (MQ=255)
                                         tgctCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTg                                                        >  1:4030574/1‑101 (MQ=255)
                                         tgctCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTg                                                        >  1:900703/1‑101 (MQ=255)
                                         tgctCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTg                                                        >  2:4644863/1‑101 (MQ=255)
                                           ctctTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGa                                                      <  2:6247095/101‑1 (MQ=255)
                                           ctctTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGa                                                      <  1:689113/101‑1 (MQ=255)
                                           ctctTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGa                                                      <  1:6684956/101‑1 (MQ=255)
                                            cctTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGAc                                                     <  1:3140198/100‑1 (MQ=255)
                                             ctTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACg                                                    <  2:1043863/101‑1 (MQ=255)
                                             ctTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACg                                                    >  2:6866324/1‑101 (MQ=255)
                                                 gCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGa                                                <  2:1362276/101‑1 (MQ=255)
                                                 gCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGa                                                >  1:2008793/1‑101 (MQ=255)
                                                   cGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGAtg                                              <  2:5942206/101‑1 (MQ=255)
                                                   cGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGAtg                                              >  2:5715393/1‑101 (MQ=255)
                                                    gTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGAtgt                                             >  1:2362670/1‑101 (MQ=255)
                                                        cgcAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTg                                         <  1:2185194/101‑1 (MQ=255)
                                                           aaTCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTg                                      >  2:780820/1‑101 (MQ=255)
                                                           aaTCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTg                                      <  2:691695/101‑1 (MQ=255)
                                                           aaTCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTg                                      >  2:6578956/1‑101 (MQ=255)
                                                            aTCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGc                                     <  1:2974175/101‑1 (MQ=255)
                                                             tCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCg                                    <  2:2908777/101‑1 (MQ=255)
                                                                gATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCt                                 >  1:480038/1‑101 (MQ=255)
                                                                gATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCt                                 >  2:6983432/1‑101 (MQ=255)
                                                                gATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCt                                 <  2:222769/101‑1 (MQ=255)
                                                                  tGCCAACCGCGCCCGTGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTgg                               >  1:1641612/1‑101 (MQ=255)
                                                                   gCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGgc                              >  2:6519118/1‑101 (MQ=255)
                                                                   gCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGgc                              <  1:3990777/101‑1 (MQ=255)
                                                                      aaCCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCt                           <  2:2990599/101‑1 (MQ=255)
                                                                      aaCCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCt                           <  2:205167/101‑1 (MQ=255)
                                                                        ccGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTgg                         >  1:342470/1‑101 (MQ=255)
                                                                           cgcCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATg                      <  2:900679/101‑1 (MQ=255)
                                                                            gcCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGc                     >  2:1475148/1‑101 (MQ=255)
                                                                              ccGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCa                   <  2:3168640/101‑1 (MQ=255)
                                                                               cGGGCGAAACTGGCCGCTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTCCGTCTGGCGCTGGATGCCAg                  >  1:5624983/1‑101 (MQ=255)
                                                                               cGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAg                  <  1:3196148/101‑1 (MQ=255)
                                                                                gggCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAgc                 >  2:4286309/1‑101 (MQ=255)
                                                                                  gCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAgcgc               >  2:2493383/1‑101 (MQ=255)
                                                                                   cGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGcc              >  1:385369/1‑101 (MQ=255)
                                                                                     aaaCTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGccag            >  2:5598321/1‑99 (MQ=255)
                                                                                     aaaCTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCgg            <  2:5624693/101‑1 (MQ=255)
                                                                                     aaaCTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCgg            >  1:225473/1‑101 (MQ=255)
                                                                                       aCTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCgggg          >  1:5166765/1‑101 (MQ=255)
                                                                                        cTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGc         <  2:1106235/101‑1 (MQ=255)
                                                                                        cTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGc         <  1:5948984/101‑1 (MQ=255)
                                                                                        cTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGc         >  1:2900908/1‑101 (MQ=255)
                                                                                         tGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGcc        >  2:5428317/1‑101 (MQ=255)
                                                                                           gCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTc      <  2:4070014/101‑1 (MQ=255)
                                                                                           gCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTc      >  1:3524502/1‑101 (MQ=255)
                                                                                            ccACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTCg     <  1:780844/101‑1 (MQ=255)
                                                                                              cctgatgCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTCGCa   <  1:6585452/100‑1 (MQ=255)
                                                                                                  |                                                                                                 
GCTGGTGGCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGCTGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTCGCAA  >  NC_014034/3216038‑3216233

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG