Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,191 A→C 96.3% K445T (AAG→ACG)  recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1910A→C96.3% 12.5 ‑3.5 0.0217K445T (AAG→ACG) recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (112/97):  ref base (1/7):  total (113/104)
Fisher's exact test strand distribution p-value = 2.96e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

GGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCA.G.TGGAA.CCGCGATGG.AA.GGCT.GCCGA.G.T.GTTCGCCCGGCAGGGCGC.G.GCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAC  >  NC_014034/3256108‑3256278
                                                                                         |                                                                                               
ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.t                                                                                  <  2:4075793/101‑1 (MQ=255)
 gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.tt                                                                                 <  2:5784712/101‑2 (MQ=255)
  ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGG.CACGGCTGGCCGA.g.ttg                                                                                <  1:415225/101‑3 (MQ=255)
    gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgct                                                                              >  1:5440643/1‑97 (MQ=255)
    gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgct                                                                              <  2:3233992/101‑5 (MQ=255)
     aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGTA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctc                                                                             >  1:5102091/1‑96 (MQ=255)
     aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctc                                                                             >  1:5012577/1‑96 (MQ=255)
       gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctcgc                                                                           >  1:7097490/1‑94 (MQ=255)
        ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctcgcc                                                                          >  2:4307736/1‑93 (MQ=255)
        ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctcgcc                                                                          >  2:5490081/1‑93 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGccccg                                                                       >  2:1194063/1‑99 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCgg                                                                       >  2:444503/1‑101 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCgg                                                                       >  2:4740196/1‑101 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCgg                                                                       >  2:5129561/1‑101 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGG.CACGGCTGGCCGA.GTT.GCTCGCCCgg                                                                       <  1:5530528/101‑1 (MQ=255)
            tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGc                                                                      <  2:2211948/101‑1 (MQ=255)
             ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGcc                                                                     >  1:2707928/1‑100 (MQ=255)
             ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCa                                                                     <  2:2454240/101‑1 (MQ=255)
             ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCa                                                                     <  1:1955535/101‑1 (MQ=255)
              tGCTCAAGGGCGGCGGGCGCGGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAg                                                                    <  2:1024542/101‑1 (MQ=255)
              tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGccg                                                                    >  1:1579198/1‑99 (MQ=255)
              tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAg                                                                    >  2:6542974/1‑101 (MQ=255)
                cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAggg                                                                  >  2:620040/1‑101 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGAGG.T.GCTCGcccccccgggg                                                                 >  1:3758389/1‑93 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCCgggg                                                                 >  2:3332036/1‑100 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAgggg                                                                 >  2:5646655/1‑100 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGgc                                                                 <  1:5873863/101‑1 (MQ=255)
                  cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGgcg                                                                >  2:4428744/1‑101 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GGTCGCCCGGCAGGgcgc                                                               >  2:2064687/1‑101 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCCGGgggc                                                               >  2:4251784/1‑101 (MQ=255)
                     gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGC.g.g                                                           <  1:179628/101‑1 (MQ=255)
                       gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGC.g.ggc                                                         <  1:7090337/101‑3 (MQ=255)
                        cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGC.g.ggca                                                        >  1:4742884/1‑98 (MQ=255)
                        cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGATGGCCGA.GTT.GCTCGCCCGGCAGGGCGC.g.ggta                                                        <  1:6255886/101‑4 (MQ=255)
                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGC.g.ggcac                                                       <  2:1659775/101‑5 (MQ=255)
                           cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCAc.gg                                                     <  1:5776530/101‑1 (MQ=255)
                           cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCAc.gg                                                     >  1:2304350/1‑101 (MQ=255)
                            gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGGGC.g.gggccggg                                                    >  1:4581063/1‑94 (MQ=255)
                            gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCAc.ggg                                                    >  2:2654077/1‑100 (MQ=255)
                            gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCAc.ggg                                                    <  2:2707826/101‑2 (MQ=255)
                            gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCAc.ggg                                                    >  1:6572999/1‑100 (MQ=255)
                            gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCAc.ggg                                                    <  1:1858567/101‑2 (MQ=255)
                                aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCAc.gggcggc                                                <  2:4656725/101‑6 (MQ=255)
                                 ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCCGCAGGGCGCGG.GCACGGGcggcc                                                >  2:5419697/1‑101 (MQ=255)
                                  ctcATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGcggccc                                               <  2:4392502/99‑1 (MQ=255)
                                  cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGcggccc                                               <  1:5146439/101‑1 (MQ=255)
                                  cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGcggccc                                               >  1:884256/1‑101 (MQ=255)
                                  cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGcggccc                                               <  1:1513943/101‑1 (MQ=255)
                                   gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCCGCAGGGCGCGG.GCAc.gggcagatcg                                             >  2:7075569/1‑93 (MQ=255)
                                    cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGcggcccgg                                             <  2:6862623/101‑1 (MQ=255)
                                     aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGgggcccggc                                            >  1:1686892/1‑101 (MQ=255)
                                     aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGcgggccggc                                            >  2:5303825/1‑101 (MQ=255)
                                     aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGcggcccggc                                            >  2:365933/1‑101 (MQ=255)
                                     aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGcggcccggc                                            <  2:300234/101‑1 (MQ=255)
                                      tGGCCGCGGGTCTGACCCTGGCCGAGGCGCATC.TGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGcggcccggcc                                           <  1:6330146/101‑1 (MQ=255)
                                      tGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGcggcccggcc                                           <  2:3722726/101‑1 (MQ=255)
                                      tGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGcggcccggcc                                           <  2:874705/101‑1 (MQ=255)
                                      tGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGcggcccggcc                                           <  1:1027345/101‑1 (MQ=255)
                                       ggCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGGGGCCCGGCCg                                          >  2:4790322/1‑101 (MQ=255)
                                           gcgGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCt                                      <  2:2302195/101‑1 (MQ=255)
                                            cgGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGGTCTg                                     >  1:6620808/1‑101 (MQ=255)
                                            cgGGTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTg                                     <  2:6885095/101‑1 (MQ=255)
                                               gTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGGCGATCTGCgg                                  >  2:6301501/1‑101 (MQ=255)
                                               gTCTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCgg                                  >  1:2432378/1‑101 (MQ=255)
                                                 cTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGGCCGGCCGATCTGCGGcc                                >  1:1148302/1‑100 (MQ=255)
                                                 cTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGGTCTGCgggc                                >  1:6938435/1‑99 (MQ=255)
                                                 cTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCt                                >  2:1303242/1‑101 (MQ=255)
                                                 cTGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCt                                <  1:3988161/101‑1 (MQ=255)
                                                  tGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTg                               <  2:1792899/101‑1 (MQ=255)
                                                  tGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTg                               <  2:3258976/101‑1 (MQ=255)
                                                  tGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTg                               <  1:4453264/101‑1 (MQ=255)
                                                  tGACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCCGCCGGGCTGCGGGTg                               >  2:3855382/1‑101 (MQ=255)
                                                   gACCCTGGCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTgg                              <  2:1358999/101‑1 (MQ=255)
                                                     ccctggCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                            <  1:3332152/96‑1 (MQ=255)
                                                       ctggCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGGCgg                          >  1:319917/4‑101 (MQ=255)
                                                         ggCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGc                        >  1:1186274/2‑101 (MQ=255)
                                                              gaggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCt                    <  1:2174913/91‑1 (MQ=255)
                                                               aggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCTg                   >  2:636234/10‑101 (MQ=255)
                                                               aggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                   <  2:1833892/92‑1 (MQ=255)
                                                               aggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                   <  1:6239184/92‑1 (MQ=255)
                                                                ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                  <  1:4868017/93‑1 (MQ=255)
                                                                 gcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.G.TCGCTCGCCCGGCAGGGCGCGG.GCACGGGTGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                 <  1:6414638/94‑1 (MQ=255)
                                                                  cgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATg                <  2:315223/95‑1 (MQ=255)
                                                                    cagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCCGGTGcc              >  2:6266239/5‑101 (MQ=255)
                                                                    cagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc              <  2:4469926/97‑1 (MQ=255)
                                                                    cagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc              <  1:2942168/97‑1 (MQ=255)
                                                                     agctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGCCg             >  1:2841681/4‑101 (MQ=255)
                                                                      gctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGGCgg            >  1:6047495/3‑101 (MQ=255)
                                                                      gctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcccg            >  2:5226298/3‑99 (MQ=255)
                                                                       ctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCTGGTGGCggg           >  2:1904900/2‑101 (MQ=255)
                                                                        tGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCCCGGGCCGCCCCGCCGAACTGCGGCTGGACGGGCTCCCGATTCCgggg          >  1:3659866/1‑100 (MQ=255)
                                                                         ggaacccGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgcg         >  1:2550395/6‑101 (MQ=255)
                                                                         ggaacccGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCCATCTGCGGCTGGGCGGGGTCCTGATGCCGGgcg         >  1:2415221/6‑101 (MQ=255)
                                                                          gaacccGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGCCGGgcgc        >  1:3561326/5‑101 (MQ=255)
                                                                          gaacccGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCTGATGCCGGgcgc        >  1:1438018/5‑101 (MQ=255)
                                                                              ccGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGC.GCGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCcgcg    <  2:1124075/101‑1 (MQ=255)
                                                                               cgcgATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGCCCGGGGCCCCGa   >  1:1070471/1‑101 (MQ=255)
                                                                                gcgATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCTGATGCCGGGcgccgccgc  >  2:801896/1‑98 (MQ=255)
                                                                                gcgATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc  <  1:2654173/101‑1 (MQ=255)
                                                                                gcgATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc  <  1:4287288/101‑1 (MQ=255)
                                                                                         |                                                                                               
GGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCA.G.TGGAA.CCGCGATGG.AA.GGCT.GCCGA.G.T.GTTCGCCCGGCAGGGCGC.G.GCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAC  >  NC_014034/3256108‑3256278

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG