Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,204 T→C 100% C449C (TGT→TGC recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,2040T→C100.0% 775.3 207C449C (TGT→TGCrecJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (96/111):  ref base (0/0):  total (96/111)

Only 100 of 221 total aligned reads displayed.

CTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.G.CCGAGG.GCAG.TGGAA.CCGCGATG.G.AA.GGCT.GCCGA.G.TGTTCGCCCGGCA..GGGCGC.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGA  >  NC_014034/3256118‑3256293
                                                                                                 |                                                                                             
cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.G.CACGGCTGGCCGA.GTTGCTCGCCCg                                                                                        <  2:4008791/101‑1 (MQ=255)
 ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCgg                                                                                       >  2:5129561/1‑101 (MQ=255)
   ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCa                                                                                     <  1:1732741/101‑1 (MQ=255)
   ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCa                                                                                     <  1:1955535/101‑1 (MQ=255)
    tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GTAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..g                                                                                  <  1:5056162/101‑1 (MQ=255)
     gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGGA..gg                                                                                 >  2:1027310/1‑101 (MQ=255)
      cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..ggg                                                                                >  2:620040/1‑101 (MQ=255)
       tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGAGG.TGCTCGcccccccgggg                                                                                 >  1:3758389/1‑93 (MQ=255)
       tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCC..gggg                                                                               >  2:3332036/1‑100 (MQ=255)
       tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..cggg                                                                               >  2:1811227/1‑100 (MQ=255)
       tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGgc                                                                               <  1:5873863/101‑1 (MQ=255)
       tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGgc                                                                               <  1:2516932/101‑1 (MQ=255)
        cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGgcg                                                                              >  1:2788651/1‑101 (MQ=255)
         aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCC..GGgggc                                                                             >  2:4251784/1‑101 (MQ=255)
             gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGC.gggc                                                                        <  1:7090337/101‑3 (MQ=255)
              cggcggGGACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCCGCA..GGGCGC.gggga                                                                       >  2:6238865/1‑98 (MQ=255)
               ggcggGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCC..GGGGGC.ggggac                                                                      >  2:4429733/1‑97 (MQ=255)
               ggcggGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGC.ggggcc                                                                      >  2:3702563/1‑97 (MQ=255)
               ggcggGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGC.gggcac                                                                      <  2:4461673/101‑5 (MQ=255)
                gcggGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGC.gggcacg                                                                     <  2:5719663/101‑6 (MQ=255)
                 cggGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCAc.gg                                                                    >  1:2304350/1‑101 (MQ=255)
                  gggCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGGGC.ggggccggg                                                                   >  1:4581063/1‑94 (MQ=255)
                   ggCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCAc.gggc                                                                  <  2:1497878/101‑3 (MQ=255)
                      aCCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCAc.gggcggc                                                               <  2:2231129/101‑6 (MQ=255)
                      aCCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCAc.gggcggc                                                               <  2:4656725/101‑6 (MQ=255)
                        ctcATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggccc                                                              <  2:4392502/99‑1 (MQ=255)
                        cGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggccc                                                              <  2:206261/101‑1 (MQ=255)
                        cGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggccc                                                              >  1:884256/1‑101 (MQ=255)
                        cGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggccc                                                              <  1:5146439/101‑1 (MQ=255)
                         gCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccc                                                             >  1:763579/1‑100 (MQ=255)
                          cATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccgg                                                            <  1:1535008/101‑1 (MQ=255)
                          cATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccgg                                                            <  2:6862623/101‑1 (MQ=255)
                           aTGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcgggccggc                                                           >  2:5303825/1‑101 (MQ=255)
                           aTGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccggc                                                           <  2:300234/101‑1 (MQ=255)
                            tGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGGGCGG.GGCCG.GGcggcccggcc                                                          >  2:2680569/1‑101 (MQ=255)
                            tGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccggcc                                                          <  2:6387128/101‑1 (MQ=255)
                            tGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccggcc                                                          <  2:874705/101‑1 (MQ=255)
                            tGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccggcc                                                          <  1:1027345/101‑1 (MQ=255)
                            tGGCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccggcc                                                          <  1:3987319/101‑1 (MQ=255)
                            tGGCCGCGGGTCTGACCCT.GTCCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccggcc                                                          <  1:5609008/101‑1 (MQ=255)
                             ggCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGGGGCCCGGCCg                                                         >  2:4790322/1‑101 (MQ=255)
                             ggCCGCGGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCg                                                         <  1:3559572/101‑1 (MQ=255)
                               gatntngTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGAt                                                       <  1:3143683/95‑1 (MQ=255)
                                 gcgGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCt                                                     >  2:5421732/1‑101 (MQ=255)
                                  cgGGTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGGTCTg                                                    >  1:6620808/1‑101 (MQ=255)
                                   gggTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGGACGGGCGGGCCGGCCGGTCTgg                                                   >  2:6305736/1‑100 (MQ=255)
                                    ggTCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGGCCCGCCGacacgcc                                                  >  2:6922646/1‑95 (MQ=255)
                                      tCTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGc                                                >  1:4795129/1‑101 (MQ=255)
                                       cTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGGCCGGCCGATCTGCGGcc                                               >  1:1148302/1‑100 (MQ=255)
                                       cTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCt                                               <  1:2556754/101‑1 (MQ=255)
                                       cTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCt                                               <  1:99969/101‑1 (MQ=255)
                                       cTGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCt                                               >  2:1303242/1‑101 (MQ=255)
                                        tgcccctgg.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTg                                              <  2:4614548/93‑1 (MQ=255)
                                        tGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTg                                              <  1:4038349/101‑1 (MQ=255)
                                        tGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTg                                              <  1:2683900/101‑1 (MQ=255)
                                        tGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTg                                              <  1:4453264/101‑1 (MQ=255)
                                        tGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTg                                              >  1:627363/1‑101 (MQ=255)
                                        tGACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCCGCCGGGCTGCGGGTg                                              >  2:3855382/1‑101 (MQ=255)
                                         gACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTgg                                             <  2:1358999/101‑1 (MQ=255)
                                         gACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTgg                                             <  1:192576/101‑1 (MQ=255)
                                         gACCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTgg                                             <  2:2788549/101‑1 (MQ=255)
                                          aCCCTGG.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGa                                            <  2:6279196/101‑1 (MQ=255)
                                           ccctgg.CCGAGGCGCAGCTGGAACCCGCGATGTC.AC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                                           <  1:1610082/96‑1 (MQ=255)
                                           ccctgg.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                                           <  2:3239562/96‑1 (MQ=255)
                                            cctgg.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCg                                          >  1:5490030/5‑101 (MQ=255)
                                             ctgg.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCgg                                         >  1:1833956/4‑101 (MQ=255)
                                               gg.CCGAGGCGCAGCTGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGc                                       >  1:1186274/2‑101 (MQ=255)
                                                    gaggcgcagctGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCt                                   <  1:5419969/91‑1 (MQ=255)
                                                    gaggcgcagctGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCt                                   <  2:117851/91‑1 (MQ=255)
                                                    gaggcgcagctGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCt                                   <  1:2174913/91‑1 (MQ=255)
                                                     aggcgcagctGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                                  <  1:6239184/92‑1 (MQ=255)
                                                        cgcagctGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATg                               <  2:315223/95‑1 (MQ=255)
                                                        cgcagctGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATg                               <  2:210820/95‑1 (MQ=255)
                                                          cagctGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc                             <  1:6213902/97‑1 (MQ=255)
                                                           agctGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCGCCTGCTGCCg                            >  2:1837629/4‑101 (MQ=255)
                                                            gctGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGGCgg                           >  1:6047495/3‑101 (MQ=255)
                                                            gctGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcccg                           >  2:5226298/3‑99 (MQ=255)
                                                            gctGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCgg                           <  1:3212665/99‑1 (MQ=255)
                                                            g.TGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCggg                          <  2:2304255/101‑1 (MQ=255)
                                                              tGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCCCGGGCCGCCCCGCCGAACTGCGGCTGGACGGGCTCCCGATTCCgggg                         >  1:3659866/1‑100 (MQ=255)
                                                              tGGAACCCGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGCCcggg                         >  2:4300938/1‑100 (MQ=255)
                                                               ggaacccGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCCATCTGCGGCTGGGCGGGGTCCTGATGCCGGgcg                        >  1:2415221/6‑101 (MQ=255)
                                                                gaacccGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCTGATGCCGGgcgc                       >  1:1438018/5‑101 (MQ=255)
                                                                 aacccGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                      <  1:6587788/98‑1 (MQ=255)
                                                                   cccGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccgc                    <  1:6543305/100‑1 (MQ=255)
                                                                    ccGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCcgcg                   <  2:7097116/101‑1 (MQ=255)
                                                                    ccGCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCcgcg                   <  2:2473274/101‑1 (MQ=255)
                                                                     cgcgATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGCCCGGGGCCCCGa                  >  1:1070471/1‑101 (MQ=255)
                                                                     cgcgATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGa                  >  1:4675287/1‑101 (MQ=255)
                                                                      gcgATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCTGATGCCGGGcgccgccgc                 >  2:801896/1‑98 (MQ=255)
                                                                             ggcacggctggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCcg            <  2:5012325/91‑1 (MQ=255)
                                                                              gcacggctggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCcgc           <  1:254859/92‑1 (MQ=255)
                                                                                    ctggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGAt     <  1:2912030/98‑1 (MQ=255)
                                                                                    ctggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCCCCTGGTGCCGGGCGCCGCGGCGCCCGGGCTggc     >  1:1502431/4‑99 (MQ=255)
                                                                                     tggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGGCGCGACGCCCGCGCGGATc    >  1:6944759/3‑101 (MQ=255)
                                                                                     tggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc    >  1:4902351/3‑101 (MQ=255)
                                                                                     tggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc    >  2:6693539/3‑101 (MQ=255)
                                                                                      ggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCg   >  2:5630786/2‑101 (MQ=255)
                                                                                      ggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCg   <  1:4251979/100‑1 (MQ=255)
                                                                                       gCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGa  >  1:637147/1‑101 (MQ=255)
                                                                                                 |                                                                                             
CTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.G.CCGAGG.GCAG.TGGAA.CCGCGATG.G.AA.GGCT.GCCGA.G.TGTTCGCCCGGCA..GGGCGC.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGA  >  NC_014034/3256118‑3256293

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG