Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,273,144 C→T 1.4% H334Y (CAT→TAT)  RCAP_rcc03079 → capsule polysaccharide biosynthesis protein family

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,273,1440C→T 1.4% 2.2 0.0 ‑0.1288H334Y (CAT→TAT) RCAP_rcc03079capsule polysaccharide biosynthesis protein family
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (135/149):  total (137/151)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.55e-01

Only 100 of 299 total aligned reads displayed.

GACCTGGGTCGACCCCTGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTGCGCTTCGTCGATCCGCCCGACCGCGCCGC  >  NC_014034/3273044‑3273244
                                                                                                    |                                                                                                    
gACCTGGGTCGACCCCTGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGcc                                                                                                      <  1:4042825/101‑1 (MQ=255)
   cTGGGTCGACCCCTGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATg                                                                                                   >  2:2331198/1‑101 (MQ=255)
    tGGGTCGACCCCTGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGt                                                                                                  >  1:907468/1‑101 (MQ=255)
     gggTCGACCCCTGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTc                                                                                                 >  2:7015806/1‑101 (MQ=255)
     gggTCGACCCCTGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTc                                                                                                 <  1:1517685/101‑1 (MQ=255)
        tCGCCCCCTGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGcc                                                                                              <  2:1400634/101‑1 (MQ=255)
        tCGCCCCCTGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGcc                                                                                              <  1:2770521/101‑1 (MQ=255)
        tCGACCCCTGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGcc                                                                                              >  1:6565344/1‑101 (MQ=255)
        tCGACCCCTGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGcc                                                                                              >  1:5683385/1‑101 (MQ=255)
         cGACCCCTGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCa                                                                                             >  2:5453281/1‑101 (MQ=255)
          gcccccTGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCAc                                                                                            <  1:5115674/99‑1 (MQ=255)
           cccccTGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCAcc                                                                                           <  2:4083786/100‑1 (MQ=255)
           cccccTGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCAcc                                                                                           <  1:465724/100‑1 (MQ=255)
           aCCCCTGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCAcc                                                                                           >  2:3712088/1‑101 (MQ=255)
           aCCCCTGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCAcc                                                                                           <  1:2364587/101‑1 (MQ=255)
            ccccTGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCg                                                                                          <  1:3986096/101‑1 (MQ=255)
             cccGGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCgg                                                                                         <  2:3561866/101‑1 (MQ=255)
              ccTGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGc                                                                                        >  1:5605060/1‑101 (MQ=255)
              ccTGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGc                                                                                        <  2:4991285/101‑1 (MQ=255)
              ccTGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGc                                                                                        >  2:6413162/1‑101 (MQ=255)
              ccTGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGc                                                                                        >  2:86514/1‑101 (MQ=255)
                tGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCAt                                                                                      <  1:311743/101‑1 (MQ=255)
                tGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCAt                                                                                      <  1:2511872/101‑1 (MQ=255)
                  ccGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGc                                                                                    >  2:1211828/1‑101 (MQ=255)
                      ggCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCt                                                                                <  1:4507308/101‑1 (MQ=255)
                      ggCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCt                                                                                <  1:1163745/101‑1 (MQ=255)
                      ggCCGGATCTGCAGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCt                                                                                >  1:6394804/1‑101 (MQ=255)
                       gCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCtg                                                                               >  2:6884437/1‑101 (MQ=255)
                         cGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCggatacc                                                                             <  2:289346/101‑6 (MQ=255)
                          ggATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTgg                                                                            >  1:366166/1‑101 (MQ=255)
                          ggATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTgg                                                                            >  1:351748/1‑101 (MQ=255)
                          ggATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTgg                                                                            <  2:6304949/101‑1 (MQ=255)
                           gATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGCGGa                                                                           >  2:1545612/1‑101 (MQ=255)
                              cTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAAc                                                                        <  1:2801436/101‑1 (MQ=255)
                              cTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAAc                                                                        <  1:3771225/101‑1 (MQ=255)
                               tGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACg                                                                       <  1:5365283/101‑1 (MQ=255)
                                   gCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGt                                                                   <  2:6132462/101‑1 (MQ=255)
                                    cTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTc                                                                  >  2:1785506/1‑101 (MQ=255)
                                     ttCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTcc                                                                 <  2:6516850/101‑1 (MQ=255)
                                      tCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCTATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCg                                                                >  1:3593384/1‑101 (MQ=255)
                                       cGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGc                                                               >  1:5822850/1‑101 (MQ=255)
                                       cGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGc                                                               >  2:1016543/1‑101 (MQ=255)
                                       cGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGc                                                               >  1:85816/1‑101 (MQ=255)
                                       cGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGc                                                               >  1:6265947/1‑101 (MQ=255)
                                           gAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTgca                                                           >  1:4957043/1‑101 (MQ=255)
                                            aaGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTgcag                                                          >  2:2555149/1‑101 (MQ=255)
                                              gCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTgcagca                                                        <  2:2506673/101‑1 (MQ=255)
                                              gCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTgcagca                                                        >  1:5524579/1‑101 (MQ=255)
                                               ccGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGcagcag                                                       <  2:6814864/101‑1 (MQ=255)
                                                cGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGt                                                      >  1:5981482/1‑101 (MQ=255)
                                                 gTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGtt                                                     <  2:3169531/101‑1 (MQ=255)
                                                  tCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGttt                                                    <  2:3931915/101‑1 (MQ=255)
                                                   cGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGtttt                                                   <  2:283197/101‑1 (MQ=255)
                                                   cGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGtttt                                                   >  2:3836067/1‑101 (MQ=255)
                                                   cGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGcttt                                                   >  2:4449652/1‑101 (MQ=255)
                                                    gATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGttttt                                                  >  2:5599111/1‑101 (MQ=255)
                                                    gATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGttttt                                                  <  1:243103/101‑1 (MQ=255)
                                                    caTCAGAGTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGttttt                                                  <  1:1285583/100‑1 (MQ=255)
                                                     aTCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTc                                                 >  1:6083169/1‑101 (MQ=255)
                                                        aGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGc                                              <  1:6670389/101‑1 (MQ=255)
                                                         gCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGcc                                             >  2:5775106/1‑101 (MQ=255)
                                                          cTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCcg                                            <  2:5874164/101‑1 (MQ=255)
                                                            tGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCcgcg                                          <  1:227577/101‑1 (MQ=255)
                                                            tGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCcgcg                                          >  2:7086244/1‑101 (MQ=255)
                                                            tGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCcgcg                                          >  2:743591/1‑101 (MQ=255)
                                                             gAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGa                                         >  1:4422423/1‑101 (MQ=255)
                                                              aGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGaa                                        <  2:6006257/101‑1 (MQ=255)
                                                               ggCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCTATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGaaa                                       <  2:3593248/101‑1 (MQ=255)
                                                               ggCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCTATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGaaa                                       <  2:4032430/101‑1 (MQ=255)
                                                                  cGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGc                                    >  2:1881913/1‑101 (MQ=255)
                                                                   gAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGcc                                   <  1:2652768/101‑1 (MQ=255)
                                                                   gAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGcc                                   >  2:143174/1‑101 (MQ=255)
                                                                    gggTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGccc                                  <  1:2088045/100‑1 (MQ=255)
                                                                    aGGTCCGCGCCTTTCGCGCCGCCCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGccc                                  <  1:1881985/101‑1 (MQ=255)
                                                                     ggTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCt                                 >  2:1745523/1‑101 (MQ=255)
                                                                       tCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTg                               <  2:5549737/101‑1 (MQ=255)
                                                                        ccGCGCCTTTCGCGCCGCCCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTgc                              <  2:5365044/101‑1 (MQ=255)
                                                                        ccGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTgc                              <  1:2858705/101‑1 (MQ=255)
                                                                        ccGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTgc                              <  2:4567299/101‑1 (MQ=255)
                                                                         cgcgCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTgcg                             <  2:3249490/101‑1 (MQ=255)
                                                                            gcCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTGCGCtt                          <  1:6802077/101‑1 (MQ=255)
                                                                             ccTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTGCGCTtc                         <  1:5418108/101‑1 (MQ=255)
                                                                             ccTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTGCGCTtc                         <  2:4286054/101‑1 (MQ=255)
                                                                             ccTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTGCGCTtc                         <  1:6963824/101‑1 (MQ=255)
                                                                                   gcgcCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTGCGCTTCGTCGAt                   <  2:1767790/101‑1 (MQ=255)
                                                                                     gcCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTGCGCTTCGTCGATcc                 >  2:2661474/1‑101 (MQ=255)
                                                                                        gACCGGGCGGGCCATGTCGCCACCGGCATGCGGGTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTGCGCTTCGTCGATCCGcc              >  1:5022351/1‑101 (MQ=255)
                                                                                        gACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTGCGCTTCGTCGATCCGcc              >  1:3455409/1‑101 (MQ=255)
                                                                                        gACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTGCGCTTCGTCGATCCGcc              <  1:795005/101‑1 (MQ=255)
                                                                                        gACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTGCGCTTCGTCGATCCGcc              >  2:3362613/1‑101 (MQ=255)
                                                                                        gACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTGCGCTTCGTCGATCCGcc              <  1:426175/101‑1 (MQ=255)
                                                                                         aCCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTGCGCTTCGTCGATCCGccc             <  2:4794582/101‑1 (MQ=255)
                                                                                          ccGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTGCGCTTCGTCGATCCGCCCg            <  1:2946257/101‑1 (MQ=255)
                                                                                           cgggcgggCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTGCGCTTCGTCGATCCGCCCGa           >  2:6670356/1‑101 (MQ=255)
                                                                                            gggcgggcCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTGCGCTTCGTCGATCCGCCCGAc          >  1:1212448/1‑101 (MQ=255)
                                                                                             ggcgggcCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTGCGCTTCGTCGATCCGCCCGAcc         >  2:3690938/1‑101 (MQ=255)
                                                                                             ggcgggcCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTGCGCTTCGTCGATCCGCCCGAcc         <  1:4759850/101‑1 (MQ=255)
                                                                                                gggcCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTGCGCTTCGTCGATCCGCCCGACcgcg      >  1:5765007/1‑101 (MQ=255)
                                                                                                   ccATGCCCCCACCGGCTTGCGGCTGTGGAACCGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTGCGCTTCGTCGATCCGCCCGACCGcgccg   <  2:4353101/101‑1 (MQ=255)
                                                                                                    cATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTGCGCTTCGTCGATCCGCCCGACCGcgccgc  >  1:7103129/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
GACCTGGGTCGACCCCTGCCGCGGCCGGATCTGCCGCTTCGAGGAAGCCGTCGATCAGCTTGAGGCCGAGGTCCGCGCCTTTCGCGCCGACCGGGCGGGCCATGTCGCCACCGGCATGCGGCTGTGGAAACGCCGTCCGCTGCAGCAGTTTTTCGGCCGCGAAAAGCCCTTGCGCTTCGTCGATCCGCCCGACCGCGCCGC  >  NC_014034/3273044‑3273244

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG