Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,342,566	C→G	100%	R154P (CGG→CCG)	RCAP_rcc03145 ←	AsnC/Lrp family transcriptional regulator

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,342,566	0	C→G	100.0%	82.3	21	R154P (CGG→CCG)	RCAP_rcc03145	AsnC/Lrp family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (0/21):  ref base (0/0):  total (0/21)

GTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACACCCAGCACCGGGGCAGCCGCGGCCGCGGGTTGCGGCAGGACAGCT  >  NC_014034/3342468‑3342593                                                                                                   |                            gTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggc                           <  1:7120441/101‑1 (MQ=255)    tcGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggc                        <  1:1156881/101‑1 (MQ=255)    tcGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggc                        <  2:4739432/101‑1 (MQ=255)    tcGGGGCTGACCTCGTTCAGCGAGGGTTGCGGCGGCGGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGTGGCCgcggcggc                        <  1:6291687/101‑1 (MQ=255)     cGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggcc                       <  2:6700333/101‑1 (MQ=255)      ggggCTGATCTCGTTCAGCGAGGGTTGCGGCGGAAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggccg                      <  2:792551/101‑1 (MQ=255)       gggCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggccgc                     <  2:3734876/101‑1 (MQ=255)        ggCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggccgcg                    <  2:5296330/101‑1 (MQ=255)           tGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGGGCCCGAGCGGGCCTTCGGGCGAAGCCCGGCACCGGGGGCGCGGCCGGCGCgggg                 >  2:4210431/1‑100 (MQ=255)            gATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGtt                <  2:6117990/101‑1 (MQ=255)             aTCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTg               <  2:5798830/101‑1 (MQ=255)              tctcGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGc              <  2:652686/101‑1 (MQ=255)               ctcGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACAGGGGCCGCGGCGGCCGCGGGTTGCg             <  1:5227952/101‑1 (MQ=255)                tcGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCgg            <  1:6736960/101‑1 (MQ=255)                tcGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCgg            <  1:4210626/101‑1 (MQ=255)                    tCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggc        <  2:80075/101‑4 (MQ=255)                    tCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggc        <  2:1785744/101‑4 (MQ=255)                    tCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggc        <  1:6225163/101‑4 (MQ=255)                      aGCGGGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcac      <  1:4877991/101‑6 (MQ=255)                      aGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcac      <  2:5872976/101‑6 (MQ=255)                      aGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcac      <  1:85050/101‑6 (MQ=255)                      aGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcac      <  1:2557372/101‑6 (MQ=255)                       gCGGGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcacc     <  2:3527133/101‑7 (MQ=255)                       gCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcacc     <  2:4356966/101‑7 (MQ=255)                       gCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcacc     <  1:1188755/101‑7 (MQ=255)                       gCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcacc     <  2:914972/101‑7 (MQ=255)                        cGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcaccg    <  2:5298745/101‑8 (MQ=255)                        cGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcaccg    <  2:5509727/101‑8 (MQ=255)                          aGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcaccggc  <  2:3482703/101‑10 (MQ=255)                          aGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcaccggc  <  2:154180/101‑10 (MQ=255)                                                                                                   |                            GTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACACCCAGCACCGGGGCAGCCGCGGCCGCGGGTTGCGGCAGGACAGCT  >  NC_014034/3342468‑3342593 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG