Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,398,880 G→A 100% P228L (CCG→CTG)  tolQ ← TolQ protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,398,8800G→A100.0% 1187.9 303P228L (CCG→CTG) tolQTolQ protein
Reads supporting (aligned to +/- strand):  new base (134/169):  ref base (0/0):  total (134/169)

Only 100 of 321 total aligned reads displayed.

CGGCATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACGGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTCGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGC  >  NC_014034/3398782‑3398977
                                                                                                  |                                                                                                 
cGGCATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACagg                                                                                                 >  1:1402987/1‑101 (MQ=255)
 ggCATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGa                                                                                                <  2:4270769/101‑1 (MQ=255)
 ggCATGTTGGCCCTTGCCGCCGGGGCGGCGTCGGCTGCCGCCGCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGa                                                                                                <  2:2767788/101‑1 (MQ=255)
  gCATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGAt                                                                                               >  1:7898/1‑101 (MQ=255)
   cATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATg                                                                                              >  1:6985647/1‑101 (MQ=255)
   cATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATg                                                                                              <  1:2169080/101‑1 (MQ=255)
    aTGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGGCAGGGTgg                                                                                             >  2:6557300/1‑101 (MQ=255)
    aTGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATgg                                                                                             >  1:5652757/1‑101 (MQ=255)
     tGTTTGCCCGTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGt                                                                                            <  2:599113/101‑1 (MQ=255)
      gTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTc                                                                                           <  2:4506984/101‑1 (MQ=255)
       tttCCCCTTGCCCCCCCGCCGCCGTCGGCTGCCGCCTCCGCCCTTTTTCATCCCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCg                                                                                          <  2:2268040/101‑1 (MQ=255)
         tGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCgag                                                                                        >  2:463439/1‑101 (MQ=255)
         tGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCgag                                                                                        <  2:5210901/101‑1 (MQ=255)
          gCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCgaga                                                                                       >  2:818047/1‑101 (MQ=255)
            ccTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAAt                                                                                     <  1:6290959/101‑1 (MQ=255)
             cTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAAtt                                                                                    <  1:2529559/101‑1 (MQ=255)
             cTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAAtt                                                                                    >  1:402713/1‑101 (MQ=255)
               tGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATtcg                                                                                  <  2:4101817/101‑1 (MQ=255)
               tGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATtcg                                                                                  <  1:511578/101‑1 (MQ=255)
                gccgccGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATtcgt                                                                                 <  2:623781/101‑1 (MQ=255)
                   gccgcGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCgg                                                                              <  2:6727984/101‑1 (MQ=255)
                   gccgcGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCgg                                                                              <  2:2630682/101‑1 (MQ=255)
                     cgcgGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTTATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGTGCGACAGGATGGTCGAGAATTCGTCGGCg                                                                            >  1:3544836/1‑101 (MQ=255)
                       cggcggcgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGaa                                                                          <  2:3786534/101‑1 (MQ=255)
                       cggcggcgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGaa                                                                          <  2:3652833/101‑1 (MQ=255)
                       cggcggcgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGaa                                                                          >  2:244933/1‑101 (MQ=255)
                        ggcggtgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAg                                                                         >  1:841932/1‑101 (MQ=255)
                             cgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTc                                                                    <  2:6912959/101‑1 (MQ=255)
                              gTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCg                                                                   >  1:940792/1‑101 (MQ=255)
                              gTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCg                                                                   >  1:6779661/1‑101 (MQ=255)
                               tCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGt                                                                  <  1:640501/101‑1 (MQ=255)
                                cGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTa                                                                 <  2:1402937/101‑1 (MQ=255)
                                cGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTa                                                                 <  2:1817066/101‑1 (MQ=255)
                                cGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTa                                                                 <  2:1940770/101‑1 (MQ=255)
                                cGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTa                                                                 >  1:658/1‑101 (MQ=255)
                                 ggCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAg                                                                >  2:2180018/1‑101 (MQ=255)
                                  gCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGc                                                               >  1:1273510/1‑101 (MQ=255)
                                  gCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGc                                                               >  2:3714997/1‑101 (MQ=255)
                                    tGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGccc                                                             <  1:244937/101‑1 (MQ=255)
                                    tGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGccc                                                             >  1:1736960/1‑101 (MQ=255)
                                     gccgccTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCg                                                            <  2:5051312/101‑1 (MQ=255)
                                     gccgccTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCg                                                            >  2:4341356/1‑101 (MQ=255)
                                     gccgccTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCg                                                            <  1:1604966/101‑1 (MQ=255)
                                         ccTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAggg                                                        >  2:304040/1‑100 (MQ=255)
                                            ccGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGAt                                                     <  1:4590484/101‑1 (MQ=255)
                                              gCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGAtct                                                   <  1:3819788/101‑1 (MQ=255)
                                              gCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGCGAtct                                                   >  2:1285464/1‑101 (MQ=255)
                                                 cTTTTTCATCCCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgt                                                <  1:6008123/101‑1 (MQ=255)
                                                  tttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtc                                               <  2:7004294/101‑1 (MQ=255)
                                                  tttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCGGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtc                                               <  1:281716/101‑1 (MQ=255)
                                                  cttttCATCCCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtc                                               <  2:3570871/100‑1 (MQ=255)
                                                   ttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtcg                                              >  1:3358604/1‑101 (MQ=255)
                                                    tttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcgg                                             >  2:1665755/1‑101 (MQ=255)
                                                    tttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcgg                                             >  1:4249871/1‑101 (MQ=255)
                                                       catcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgt                                          <  2:3100344/101‑1 (MQ=255)
                                                       catcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgt                                          >  1:413502/1‑101 (MQ=255)
                                                        atcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtc                                         >  2:5556241/1‑101 (MQ=255)
                                                          caCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcgg                                       >  1:2710766/1‑101 (MQ=255)
                                                          caCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcgg                                       >  2:6483856/1‑101 (MQ=255)
                                                          caCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcgg                                       <  2:3834431/101‑1 (MQ=255)
                                                          caCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcgg                                       <  2:2375974/101‑1 (MQ=255)
                                                          caCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCCTACCCCGAGGTGATCTtgtcggcggccg                                       >  2:1195151/1‑97 (MQ=255)
                                                            cccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGAc                                     <  1:1016657/101‑1 (MQ=255)
                                                               ccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgag                                  <  2:6306565/101‑1 (MQ=255)
                                                                    cccATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCtt                             >  1:1941306/1‑101 (MQ=255)
                                                                     ccATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttg                            >  2:4848015/1‑101 (MQ=255)
                                                                      cATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgt                           <  2:5637628/101‑1 (MQ=255)
                                                                       aTCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgtt                          >  2:6428899/1‑101 (MQ=255)
                                                                        tCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgttg                         >  1:2288214/1‑101 (MQ=255)
                                                                         cGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTtgttgt                        <  1:737611/101‑1 (MQ=255)
                                                                         cGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTtgttgt                        <  1:6546418/101‑1 (MQ=255)
                                                                          gCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGGCGGACGAGAGCTTGTTGTa                       >  2:3298094/1‑101 (MQ=255)
                                                                          gCTTACGCGTCCAGCTGGCGCGACAGGATGGTCCAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTa                       >  1:3695747/1‑101 (MQ=255)
                                                                           cTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                      <  2:5425782/101‑1 (MQ=255)
                                                                           cTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                      >  1:2075859/1‑101 (MQ=255)
                                                                           cTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                      <  1:5043556/101‑1 (MQ=255)
                                                                           cTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                      >  2:6713294/1‑101 (MQ=255)
                                                                           cTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                      <  1:3298210/101‑1 (MQ=255)
                                                                            ttACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAgg                     >  1:4901997/1‑101 (MQ=255)
                                                                              aCGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCg                   <  1:978124/101‑1 (MQ=255)
                                                                                gctgatcacTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGAt                 >  2:4995506/9‑101 (MQ=255)
                                                                                gcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGAt                 <  1:5515977/101‑1 (MQ=255)
                                                                                gcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGAt                 >  2:5760978/1‑101 (MQ=255)
                                                                                  gTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGa               <  2:1567519/101‑1 (MQ=255)
                                                                                   tCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAc              >  2:899529/1‑101 (MQ=255)
                                                                                   tCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAc              <  2:476855/101‑1 (MQ=255)
                                                                                   tCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAc              <  2:204227/101‑1 (MQ=255)
                                                                                     cAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccg            <  2:2215456/101‑1 (MQ=255)
                                                                                      aGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgc           >  1:3215438/1‑101 (MQ=255)
                                                                                        cTGGCGCGACAGGATGGTCGCGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAcctccg         <  1:6844167/101‑1 (MQ=255)
                                                                                        cTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgccg         <  1:5882854/101‑1 (MQ=255)
                                                                                        cTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgccg         <  1:1507156/101‑1 (MQ=255)
                                                                                         tGGCGCGACAGGATGGTCGAGAATTCGTCGGAGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGGCGAGAGCTTGTTGTAGGCGATGAcctcctg        >  2:1238235/1‑99 (MQ=255)
                                                                                          ggCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCggg       >  1:397598/1‑101 (MQ=255)
                                                                                             gcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATc    <  1:6108885/101‑1 (MQ=255)
                                                                                             gcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATc    <  1:4508721/101‑1 (MQ=255)
                                                                                             gcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATc    <  1:4471452/101‑1 (MQ=255)
                                                                                             gcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATc    >  1:2636466/1‑101 (MQ=255)
                                                                                              cgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcg   >  1:528809/1‑101 (MQ=255)
                                                                                               gACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcgc  >  2:3468687/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CGGCATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACGGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTCGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGC  >  NC_014034/3398782‑3398977

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG