Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,398,929 C→T 100% E212K (GAG→AAG)  tolQ ← TolQ protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,398,9290C→T100.0% 1099.2 275E212K (GAG→AAG) tolQTolQ protein
Reads supporting (aligned to +/- strand):  new base (160/115):  ref base (0/0):  total (160/115)

Only 100 of 327 total aligned reads displayed.

CTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACGGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTCGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATCCCCGGC  >  NC_014034/3398831‑3399026
                                                                                                  |                                                                                                 
 tttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtc                                                                                                <  2:4953927/101‑1 (MQ=255)
  ttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtcg                                                                                               >  1:3358604/1‑101 (MQ=255)
  ttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtcg                                                                                               >  1:2698429/1‑101 (MQ=255)
   tttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcgg                                                                                              >  2:1665755/1‑101 (MQ=255)
      catcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgt                                                                                           <  2:3100344/101‑1 (MQ=255)
       atcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtc                                                                                          <  2:1585742/101‑1 (MQ=255)
       atcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtc                                                                                          <  1:6877235/101‑1 (MQ=255)
       atcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtc                                                                                          >  2:5556241/1‑101 (MQ=255)
         cccccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcgg                                                                                        <  1:3673760/99‑1 (MQ=255)
          ccccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGa                                                                                       <  1:6221993/100‑1 (MQ=255)
          ccccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGa                                                                                       <  1:3125305/100‑1 (MQ=255)
          aCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGa                                                                                       <  1:1687931/101‑1 (MQ=255)
          aCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGGGATCTtgtcggcgtcggc                                                                                       >  2:5651976/1‑100 (MQ=255)
           cccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGAc                                                                                      <  1:1016657/101‑1 (MQ=255)
               cgcgCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgaga                                                                                  <  1:3801829/101‑1 (MQ=255)
                gcgcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGagag                                                                                 >  2:4701929/1‑101 (MQ=255)
                gcgcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGagag                                                                                 <  2:5593802/101‑1 (MQ=255)
                 cgcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGc                                                                                >  2:3162494/1‑101 (MQ=255)
                    ccATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttg                                                                             <  2:6238966/101‑1 (MQ=255)
                    ccATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttg                                                                             >  2:4848015/1‑101 (MQ=255)
                      aTCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgtt                                                                           >  2:6428899/1‑101 (MQ=255)
                       tCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgttg                                                                          >  1:2288214/1‑101 (MQ=255)
                         gCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTa                                                                        >  2:1401784/1‑101 (MQ=255)
                         gCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTa                                                                        >  2:3525530/1‑101 (MQ=255)
                          cTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                                                                       <  1:3298210/101‑1 (MQ=255)
                           ttACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAgg                                                                      >  1:4901997/1‑101 (MQ=255)
                             aCGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCg                                                                    <  1:978124/101‑1 (MQ=255)
                             aCGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCg                                                                    <  1:2239921/101‑1 (MQ=255)
                             aCGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCATCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCg                                                                    >  1:5111334/1‑101 (MQ=255)
                               gcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGAt                                                                  >  1:6364064/1‑101 (MQ=255)
                               gcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGAt                                                                  >  1:4079468/1‑101 (MQ=255)
                               gcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGAt                                                                  <  1:818071/101‑1 (MQ=255)
                               gcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGAt                                                                  <  1:839539/101‑1 (MQ=255)
                                 gTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGa                                                                >  2:1498715/1‑101 (MQ=255)
                                  tCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAc                                                               <  1:6566190/101‑1 (MQ=255)
                                  tCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAc                                                               <  2:3266441/101‑1 (MQ=255)
                                  tCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAc                                                               <  2:204227/101‑1 (MQ=255)
                                       cTGGCGCGACAGGATGGTCGCGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAcctccg                                                          <  1:6844167/101‑1 (MQ=255)
                                       cTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgccg                                                          <  1:1507156/101‑1 (MQ=255)
                                        tGGCGCGACAGGATGGTCGAGAATTCGTCGGAGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGGCGAGAGCTTGTTGTAGGCGATGAcctcctg                                                         >  2:1238235/1‑99 (MQ=255)
                                         ggCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCggg                                                        >  1:715355/1‑101 (MQ=255)
                                            gcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATc                                                     <  1:4508721/101‑1 (MQ=255)
                                            gcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATc                                                     >  2:3596026/1‑101 (MQ=255)
                                            gcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATc                                                     <  1:2156601/101‑1 (MQ=255)
                                            gcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATc                                                     <  2:6726759/101‑1 (MQ=255)
                                             cgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcg                                                    <  2:4057739/101‑1 (MQ=255)
                                               acaggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcgcg                                                  >  1:1910588/4‑101 (MQ=255)
                                                 aggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGACCTTGTTGTAGGCGATGACCGCCGGGATCgcggc                                                <  1:3715147/100‑1 (MQ=255)
                                                   gATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGa                                              >  1:464613/1‑101 (MQ=255)
                                                    aTGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGAc                                             >  1:1779543/1‑101 (MQ=255)
                                                       gTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAg                                          <  2:3802745/101‑1 (MQ=255)
                                                        tCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAgg                                         >  1:4469106/1‑101 (MQ=255)
                                                         cGAGATTTCGTCGGCGAATGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCCGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAgac                                        <  2:1140912/101‑3 (MQ=255)
                                                         cGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGc                                        >  1:1865401/1‑101 (MQ=255)
                                                         cGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGc                                        >  1:1978154/1‑101 (MQ=255)
                                                          gagaATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGcc                                       >  2:5792364/1‑101 (MQ=255)
                                                          gagaATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGcc                                       >  2:3211415/1‑101 (MQ=255)
                                                            gaATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGa                                     >  2:2251869/1‑101 (MQ=255)
                                                             aaTTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGAt                                    >  2:4400031/1‑101 (MQ=255)
                                                              aTTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATc                                   >  2:5229570/1‑101 (MQ=255)
                                                              aTTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATc                                   <  2:501138/101‑1 (MQ=255)
                                                                 cgtcgGCGAAGGCCTCGTAGCCTGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGcc                                <  2:2081332/101‑1 (MQ=255)
                                                                  gtcgGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCg                               >  1:1155598/1‑101 (MQ=255)
                                                                  gtcgGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCg                               >  1:4314197/1‑101 (MQ=255)
                                                                   tcgGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGt                              <  2:5111074/101‑1 (MQ=255)
                                                                   tcgGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGt                              >  2:2345567/1‑101 (MQ=255)
                                                                     ggCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTgg                            >  2:4010965/1‑101 (MQ=255)
                                                                     ggCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTgg                            >  2:2388424/1‑101 (MQ=255)
                                                                       cGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGcc                          >  2:764190/1‑101 (MQ=255)
                                                                        gAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGcca                         >  1:2519165/1‑101 (MQ=255)
                                                                        gAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGcca                         >  2:1778130/1‑101 (MQ=255)
                                                                        gAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGcca                         >  1:2835031/1‑101 (MQ=255)
                                                                        gAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGcca                         <  2:4171839/101‑1 (MQ=255)
                                                                         aaGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGccac                        >  2:6980832/1‑101 (MQ=255)
                                                                         aaGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGccac                        <  1:6730237/101‑1 (MQ=255)
                                                                         aaGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGccac                        >  2:3907934/1‑101 (MQ=255)
                                                                         aaGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGccac                        >  1:3784505/1‑101 (MQ=255)
                                                                           ggCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGccacca                      <  1:6472011/101‑1 (MQ=255)
                                                                            gCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAg                     >  1:6095424/1‑101 (MQ=255)
                                                                                 gTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCt                <  2:2869261/101‑1 (MQ=255)
                                                                                 gTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCt                >  2:6615882/1‑101 (MQ=255)
                                                                                  tAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTc               >  2:2183901/1‑101 (MQ=255)
                                                                                  tAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTc               >  1:4120531/1‑101 (MQ=255)
                                                                                  tAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTc               >  2:3842229/1‑101 (MQ=255)
                                                                                  tAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAACGCCGATCGCCGTGGCCACCAGCGcccc               >  1:4819156/1‑99 (MQ=255)
                                                                                   aGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCg              >  2:7062476/1‑101 (MQ=255)
                                                                                   aGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCg              >  1:5977979/1‑101 (MQ=255)
                                                                                   aGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCg              <  1:4048110/101‑1 (MQ=255)
                                                                                   aGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCg              <  2:2623357/101‑1 (MQ=255)
                                                                                   aGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCg              >  2:2696980/1‑101 (MQ=255)
                                                                                   aGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCg              <  1:5313500/101‑1 (MQ=255)
                                                                                     cccGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGga            >  2:6519296/1‑101 (MQ=255)
                                                                                     cccGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGAGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGga            <  2:3360118/101‑1 (MQ=255)
                                                                                      ccGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGgag           >  1:1381279/1‑101 (MQ=255)
                                                                                      ccGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGgag           <  1:5556531/101‑1 (MQ=255)
                                                                                       cGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGgaga          >  1:5681886/1‑101 (MQ=255)
                                                                                         aGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATc        >  2:6141137/1‑101 (MQ=255)
                                                                                         aGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATc        >  2:6328761/1‑101 (MQ=255)
                                                                                              aTCTTGTCGGCGTCGGACGATAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATCCCCgg   <  1:4370180/101‑1 (MQ=255)
                                                                                              aTCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATCCCCgg   >  1:2592149/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACGGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTCGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATCCCCGGC  >  NC_014034/3398831‑3399026

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG