Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,788 A→G 5.8% intergenic (‑91/+72) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,7880A→G 5.8% 22.1 ‑0.9 0.0236intergenic (‑91/+72)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (5/9):  ref base (107/115):  total (112/124)
Fisher's exact test strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 321 total aligned reads displayed.

GCAGGCTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGC  >  NC_014034/3439688‑3439890
                                                                                                     |                                                                                                         
gcaggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATa                                                                                                           <  2:3594900/101‑1 (MQ=255)
 caggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATaa                                                                                                          <  1:3310255/101‑1 (MQ=255)
  cggcTCATGGCGGCGGCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATaaa                                                                                                         <  2:570698/100‑1 (MQ=255)
  aggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTAATTTGGGCGAGAAaaa                                                                                                         >  1:3025959/1‑101 (MQ=255)
   ggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAg                                                                                                        <  1:6992111/101‑1 (MQ=255)
   ggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAg                                                                                                        <  2:1851968/101‑1 (MQ=255)
     cTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAggg                                                                                                      <  2:5951975/101‑1 (MQ=255)
       cATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGAATTGGGGGAAGGAAAAggggg                                                                                                    >  1:180085/1‑100 (MQ=255)
        aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGCGATTGGGGGCAA.ATAAAGGGgct                                                                                                   >  1:1067935/1‑101 (MQ=255)
        aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGGGAGATAAAgggggt                                                                                                   >  2:1425174/1‑99 (MQ=255)
        aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTTGGGGGAGAGAAAgggggt                                                                                                   >  1:6548991/1‑99 (MQ=255)
        aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGgct                                                                                                   <  1:2247444/101‑1 (MQ=255)
          ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGCCAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGgcttt                                                                                                 <  1:6415768/101‑1 (MQ=255)
          ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGgcttt                                                                                                 <  2:5284846/101‑1 (MQ=255)
          ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGgcttt                                                                                                 <  1:2976170/101‑1 (MQ=255)
            cggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCCAGGTAAAGGGggtttgc                                                                                               >  1:3806375/1‑101 (MQ=255)
              gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGgctttgctt                                                                                             <  1:2723337/101‑1 (MQ=255)
              gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGgctttgctt                                                                                             <  2:2128981/101‑1 (MQ=255)
               cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGgctttgcttt                                                                                            <  2:4312888/101‑1 (MQ=255)
                gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTGGGGGGAGAGAAAAGGGGTTTGCtttt                                                                                           >  2:4502665/1‑101 (MQ=255)
                gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCtttt                                                                                           <  1:19595/101‑1 (MQ=255)
                  cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTAt                                                                                         <  1:4102175/101‑1 (MQ=255)
                  cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCCCCGGC.GGGGGTATTTGGGGGAAGATAAAGGGGGTTTGGtttttt                                                                                         >  2:4405670/1‑99 (MQ=255)
                    cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTTGGGGGAGATAAAGGGGGTTTGCtttttttt                                                                                       >  2:259565/1‑97 (MQ=255)
                    cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTagat                                                                                       >  1:116295/1‑98 (MQ=255)
                    cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCt                                                                                       <  1:752521/101‑1 (MQ=255)
                      acacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCt.tt                                                                                    <  1:1274437/101‑1 (MQ=255)
                      acacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCt.tt                                                                                    <  2:1168681/101‑1 (MQ=255)
                        ccGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTcc                                                                                  <  2:5435566/100‑1 (MQ=255)
                        acGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTTGGGCAAGATAAAGGGGCTTTGCTTTTTTTt.tttc                                                                                  >  2:6041504/1‑99 (MQ=255)
                         cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTccc                                                                                 <  1:316649/101‑1 (MQ=255)
                           gcgcGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCa                                                                               <  1:1379627/101‑1 (MQ=255)
                             gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCaaa                                                                             <  2:6427916/101‑1 (MQ=255)
                              cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAat                                                                            <  2:4677933/101‑1 (MQ=255)
                              cgACCAGCCTTCGAAAGACACGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAat                                                                            <  1:4381797/101‑1 (MQ=255)
                               gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTGGGGGAAGATAAAGGGGGGTTGGTTTTATCT.TTCCCCacaaa                                                                           >  1:6352635/1‑97 (MQ=255)
                               gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAata                                                                           <  2:4037015/101‑1 (MQ=255)
                                aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAatat                                                                          <  2:1019151/101‑1 (MQ=255)
                                aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAatat                                                                          <  1:3879407/101‑1 (MQ=255)
                                aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAatat                                                                          <  1:3729819/101‑1 (MQ=255)
                                 ccAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATc                                                                         <  1:5117395/101‑1 (MQ=255)
                                  cAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGCAAGATAAAGGGGCGTTGCTTTTTTTT.TTTCCCAAATATcc                                                                        >  1:2877760/1‑101 (MQ=38)
                                   aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATccc                                                                       <  2:6526557/101‑1 (MQ=255)
                                      tttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGGAAGATAAAGGGGGGTTTCTTTTTTTT.TTCCCCAAAAATTCCggg                                                                    >  2:5150565/1‑101 (MQ=37)
                                       ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGGAAGATAAAGGGGGTTTGCTTTTTTTT.TTTCCCCAAAATCCCgggg                                                                   >  1:6121268/1‑101 (MQ=255)
                                       ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCgggg                                                                   <  1:5534737/101‑1 (MQ=255)
                                        tCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGGAAGATAAAAGGGGGTTGCTTTTATTT.TTTCCCCAATAAACCggggg                                                                  >  1:5988167/1‑101 (MQ=255)
                                         cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGGAAGATAAAGGGGCTTTGCTTTTTTTT.TTTCCCAAATATCCCgggggg                                                                 >  1:6708010/1‑100 (MQ=255)
                                          gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCGAGATAAAGGGGCTTTGCTTTTATCT.TTTCCCCAATATCCCcgggggg                                                                >  2:6340805/1‑99 (MQ=38)
                                          gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTg                                                                <  1:4840420/101‑1 (MQ=255)
                                           aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGc                                                               <  2:6981681/101‑1 (MQ=255)
                                             aGACAGGAATTCGGCCTGCGGCCCATGCCTCCGGC.GGGCGTATTTGGGGCGAGATAAAGGGGCTTTGCTTTTATCT.TTCTCCACAAATACCTGCGGTGC..gg                                                           >  1:5876062/1‑101 (MQ=255)
                                              gACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..ggg                                                          <  2:3435200/101‑1 (MQ=255)
                                               aCAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTTTCT.TTCCCCCAATATCCCGGGGGTGC..gggg                                                         >  2:3646884/1‑101 (MQ=255)
                                                 aGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTTTCT.TTCCCCCAATATCCCCGGGGGGC..GGGGGc                                                       >  1:1124096/1‑101 (MQ=37)
                                                 aGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGc                                                       <  1:6233208/101‑1 (MQ=255)
                                                 aGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGc                                                       <  2:4240369/101‑1 (MQ=255)
                                                   gAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCCAATATCCCGGGGGGGC..GGGGGGag                                                     >  1:5348686/1‑101 (MQ=37)
                                                       tCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGGAAGATAAAGGGGCTTTGCTTTTTTCT.TTCCCCAAATATCCCGGGGGGGC..GGGGGCagaggc                                                 >  2:4142159/1‑99 (MQ=38)
                                                        cGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTTGGGGAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGccc                                                >  2:76352/1‑101 (MQ=255)
                                                        cGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTTCCCCAAAATCCCGGGGGGGG..GGGGGGAGGGccc                                                >  1:3652114/1‑101 (MQ=25)
                                                        cGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGccc                                                <  1:259569/101‑1 (MQ=255)
                                                         ggCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGcccc                                               <  2:1296618/101‑1 (MQ=255)
                                                          gCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGccccc                                              <  1:882130/101‑1 (MQ=255)
                                                           gtgactttcGATGCCTCCGGC.GGGGGTATTTTGGGCAAGATGAA.ACCCATCCCCTTCATCT.TTCCCCCAAAATCCCGGGGGGCCCGGGGGGCAGCGccccc                                              >  1:2695008/9‑101 (MQ=25)
                                                           ccTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGGCAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCCGGGGTGC..GGGGGCAGAGcccccc                                             >  2:3131638/1‑100 (MQ=255)
                                                           ccTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCcg                                             <  1:1665065/101‑1 (MQ=255)
                                                           ccTGCGGCCGATGCCGCCGGC.GGGGATATCTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCcg                                             <  1:4502904/101‑1 (MQ=255)
                                                            tgactttcGATGCCTCCGGC.GGGGATATTTGGGGCAAGATGAA.ACCCATCCCTTTCATCT.TGCCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGcccccc                                             <  2:5759785/94‑2 (MQ=25)
                                                             tGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCcgcc                                           <  1:1613442/101‑2 (MQ=255)
                                                               ctttcGATGCCTCCGGC.GGGGATATTTGGGGCAAGATGAA.ACCCATCCCTTTCATCT.TGCCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGccccccgcc                                          <  1:5462351/97‑5 (MQ=25)
                                                               ctttcGATGCCTCCGGC.GGGGATATTNGNGNCAAGATGAA.ACCCATCCCTTTCATCT.TGCCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGccccccgcc                                          <  1:4585024/97‑5 (MQ=21)
                                                               cGGCCGATGCCTCCGGC.GGGGATATTTGGGGCCAGATAAAGGGGCTTTGGTTTTATCT.TTTCCCCAATATCCCGGGGGTGG..GGGGGCCGAGGCCCcgccgc                                         >  2:1787442/1‑98 (MQ=37)
                                                                 gCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGGGC..GGGGGCAGAGCCCCcgccgcca                                       >  1:3075811/1‑96 (MQ=255)
                                                                   cGATGCCTCCGGC.GGGGATATTTTGGGCAAGATGAA.AACCCTCCCTTTTCTCT.TGCCCCAAAAATCCCGGGGGGCC..CGGGGGGGAGcgcccccccccccct                                    >  2:4841360/1‑91 (MQ=18)
                                                                      tGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGGGC..GGGGGCAGAGGCCC..CGCCGCCACGGtt                                >  2:2214242/1‑101 (MQ=18)
                                                                           gcgcggggggGTATTTTGGGGAAAATATAGGGGGGTTTTTTTTTTTT.CTTCCCAAAAATCGCGGGGGGGG..GGGGGGGCAGCCCCCCCGCCGCCACGGTTCa                              <  2:3025894/95‑1 (MQ=255)
                                                                            cggggggggTTTTTTGGGGAAAGAAAAAGGGG.TTTTTTTTTTTCTCTTCCCCAAA.ATCCCCGGGGGGG..GGGGGGGCAGCCCCCCCGCCGCCACGGTTCAcg                            <  2:1708078/96‑1 (MQ=255)
                                                                             ggc.ggGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCAcgccg                         >  2:3773995/1‑101 (MQ=255)
                                                                              gggggggGTTTTGGGGGAAAAAAAAAGGGTGTTTTTTTTTTTTC.TCCCCCAAATTCCCCGGGGGGGG..GGGGGC.CACCCCCCCCGCCGCCACGGTTCAcgcc                          <  1:2099666/98‑1 (MQ=255)
                                                                                   ggATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                    >  2:4777677/1‑101 (MQ=255)
                                                                                      tatTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtc                 >  2:7077351/1‑101 (MQ=255)
                                                                                       atTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                >  2:1452312/1‑101 (MQ=255)
                                                                                       atTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                >  2:672834/1‑101 (MQ=255)
                                                                                          tGGGGCAAGATACAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg             >  1:6318477/1‑101 (MQ=255)
                                                                                          tGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg             >  1:4182845/1‑101 (MQ=255)
                                                                                          tGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg             >  2:2804644/1‑101 (MQ=255)
                                                                                          tGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCCTCTCCg             >  2:1943699/1‑101 (MQ=255)
                                                                                               cAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc        >  1:1895652/1‑101 (MQ=255)
                                                                                               cAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc        >  1:4291677/1‑101 (MQ=255)
                                                                                                aaGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg       >  2:5572224/1‑101 (MQ=255)
                                                                                                 aGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGc      >  1:2785758/1‑101 (MQ=255)
                                                                                                 aGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGc      >  1:6813122/1‑101 (MQ=255)
                                                                                                  gATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc     >  2:1595707/1‑101 (MQ=255)
                                                                                                  gATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc     >  1:3263183/1‑101 (MQ=255)
                                                                                                  gATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc     >  2:1122311/1‑101 (MQ=255)
                                                                                                   aTAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa    >  2:2738773/1‑101 (MQ=255)
                                                                                                    tAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg   >  2:28872/1‑101 (MQ=255)
                                                                                                     aaaGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc  >  1:250897/1‑101 (MQ=255)
                                                                                                     aaaGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGCGTCGCCAgc  >  2:2652390/1‑101 (MQ=255)
                                                                                                     |                                                                                                         
GCAGGCTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGC  >  NC_014034/3439688‑3439890

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG