Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,792 G→A 5.7% intergenic (‑95/+68) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,7920G→A 5.7% 19.6 ‑1.3 ‑0.0241intergenic (‑95/+68)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (5/9):  ref base (116/111):  total (121/120)
Fisher's exact test strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.98e-01

Only 100 of 314 total aligned reads displayed.

GCTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGA  >  NC_014034/3439692‑3439894
                                                                                                      |                                                                                                             
gcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGAATTTTGGGGAAGAT.AAAgg                                                                                                               >  1:3785291/1‑101 (MQ=255)
 cTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGGAAGAG.AAAagg                                                                                                              >  1:1708139/1‑101 (MQ=39)
    aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGCGATTGGGGGCAA.AT.AAAGGGgct                                                                                                           >  1:1067935/1‑101 (MQ=255)
    aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGGGAGAT.AAAgggggt                                                                                                           >  2:1425174/1‑99 (MQ=255)
    aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTTGGGGGAGAG.AAAgggggt                                                                                                           >  1:6548991/1‑99 (MQ=255)
    aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGgct                                                                                                           <  1:2247444/101‑1 (MQ=255)
      ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGgcttt                                                                                                         <  2:5372759/101‑1 (MQ=255)
       gcggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGgctttg                                                                                                        <  2:2692575/101‑1 (MQ=255)
         ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGGCAGAT.AAAGGGgctttgc.t                                                                                                     >  2:6385392/1‑101 (MQ=255)
          gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGgctttgc.tt                                                                                                    <  2:1039143/101‑1 (MQ=255)
          gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGgctttgc.tt                                                                                                    <  1:298368/101‑1 (MQ=255)
          gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGgctttgc.tt                                                                                                    <  1:2723337/101‑1 (MQ=255)
            gACCCTACACGGCGCGGCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.tttt                                                                                                  <  2:1895917/101‑1 (MQ=255)
            gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTGGGGGGAGAG.AAAAGGGGTTTGC.tttt                                                                                                  >  2:4502665/1‑101 (MQ=255)
            gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGGAAGAT.AAAGGGGCTTTTC.tttt                                                                                                  >  1:886000/1‑101 (MQ=255)
              cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTTGGGCAAGATAAAAGGGGGTTTGT.ttttt                                                                                                 >  1:1618485/1‑100 (MQ=255)
              cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTAt                                                                                                <  1:7036369/101‑1 (MQ=255)
                cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTGGGGCAAGAG.AAAGGGGGTTTGC.TTTTAttt                                                                                              >  1:1909414/1‑99 (MQ=39)
                cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTagat                                                                                              >  1:116295/1‑98 (MQ=255)
                 tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.tt                                                                                            <  1:5911957/101‑1 (MQ=255)
                  acacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTGGGGGAAGAT.AAAAGGGGGTTGC.TTTTTTT.ttt                                                                                           >  1:6348841/1‑101 (MQ=255)
                  acacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.ttt                                                                                           <  2:1168681/101‑1 (MQ=255)
                   cacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..c                                                                                        <  2:5439453/101‑1 (MQ=255)
                      ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..cccc                                                                                     <  2:3505286/101‑1 (MQ=255)
                      ggCGCAACCAGCTTGCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..cccc                                                                                     <  2:2609785/101‑1 (MQ=255)
                       gcgcGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGAATTTTGGGGAAGAT.AAAAGGGGTTTGC.TTTTTTT.TTT..ccccc                                                                                    >  1:2001622/1‑100 (MQ=255)
                         gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCaaa                                                                                  <  1:372520/101‑1 (MQ=255)
                         gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCaaa                                                                                  <  1:4100347/101‑1 (MQ=255)
                          cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAat                                                                                 <  2:4677933/101‑1 (MQ=255)
                           gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGGAAGAT.AAAAGGGCTTTGC.TTTTTTCTTTT..CCCCaaaa                                                                                 >  1:5292946/1‑100 (MQ=38)
                           gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAata                                                                                <  2:4037015/101‑1 (MQ=255)
                              cAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGCAAGAT.AAAGGGGCGTTGC.TTTTTTT.TTT..TCCCAAATATcc                                                                             >  1:2877760/1‑101 (MQ=38)
                                  tttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGGAAGAT.AAAGGGGGGTTTC.TTTTTTT.TTT..CCCCAAAAATTCCggg                                                                         >  2:5150565/1‑101 (MQ=37)
                                   ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCgggg                                                                        <  1:5534737/101‑1 (MQ=255)
                                     cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGGAAGAT.AAAGGGGCTTTGC.TTTTTTT.TTT..TCCCAAATATCCCgggggg                                                                      >  1:6708010/1‑100 (MQ=255)
                                     cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGGTTTGC.TTTTATC.TTT..TCCCCAATATCCCgggggg                                                                      >  2:4454851/1‑100 (MQ=255)
                                       aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTGGGGGAAGAA.AAAAGGGCGTTGT.TTTTTTT.TTTTCCCCCAAATCCCCCgggggg                                                                      >  1:4763077/1‑100 (MQ=255)
                                       aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGc                                                                    <  2:6981681/101‑1 (MQ=255)
                                       aaaGACAGGAATTCGGCCTGAGGCCGATGCCTCCGGC.GGGGGTATTTGGGGCAAGAT.AAAAGGGGTTTGCTTTTTTTT.TTT..CCCCAAAAATCCGggggggg                                                                     >  1:6384628/1‑99 (MQ=25)
                                        aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..g                                                                 <  1:3082657/101‑1 (MQ=255)
                                        aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..g                                                                 <  2:6200039/101‑1 (MQ=255)
                                         aGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGAATTTGGGGGAAAAT.AAAGGGGCTTTGG.TTTTTTC.TTT..CCCCCCAAATCCCgggggggggg                                                                  >  1:1255659/1‑96 (MQ=255)
                                           aCAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGGAAGAT.AAAGGGGGTTTGC.TTTTATT.TTT..TCCCAAATATCCCGGGGGTGC..gggg                                                              >  2:5616182/1‑101 (MQ=37)
                                           aCAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTTTC.TTT..CCCCCAATATCCCGGGGGTGC..gggg                                                              >  2:3646884/1‑101 (MQ=255)
                                             aGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTTTC.TTT..CCCCCAATATCCCCGGGGGGC..GGGGGc                                                            >  1:1124096/1‑101 (MQ=37)
                                             aGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCATATATCCCGGGGGTGC..GGGGGc                                                            >  2:1546605/1‑101 (MQ=255)
                                             aGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGc                                                            <  1:3184716/101‑1 (MQ=255)
                                             aGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGc                                                            <  2:4240369/101‑1 (MQ=255)
                                             aGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGc                                                            <  1:6233208/101‑1 (MQ=255)
                                              ggAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGCAAGAT.AAAGGGGGTTTGC.TTTTATC.TTT..CCCCCAATATCCCGGGGGGGG..ggggggg                                                           >  1:679386/1‑99 (MQ=37)
                                               gAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGGAAGAT.AAAGGGGGTTTGC.TTTTATC.TTT..TCCCCAATATCCCCGGGGTGC..GGGGGGag                                                          >  2:5927015/1‑101 (MQ=255)
                                                   tCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGGAAGAT.AAAGGGGCTTTGC.TTTTTTC.TTT..CCCCAAATATCCCGGGGGGGC..GGGGGCagaggc                                                      >  2:4142159/1‑99 (MQ=38)
                                                   tCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGc.c                                                     <  2:180082/101‑1 (MQ=255)
                                                    cGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTTGGGGAAGAT.AAAGGGGGTTTGC.TTTTATC.TTT..CCCCAAATATTCCGGGGGGGC..GGGGGCAGAGg.cc                                                    >  2:645105/1‑101 (MQ=37)
                                                    cGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTTGGGGAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGc.cc                                                    >  2:76352/1‑101 (MQ=255)
                                                    cGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGc.cc                                                    <  1:259569/101‑1 (MQ=255)
                                                     ggCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGc.ccc                                                   <  2:1296618/101‑1 (MQ=255)
                                                     ggCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGc.ccc                                                   <  1:6053156/101‑1 (MQ=255)
                                                       ccTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCCcg                                                 >  1:3284537/1‑101 (MQ=255)
                                                       ccTGCGGCCGATGCCGCCGGC.GGGGATATCTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCCcg                                                 <  1:4502904/101‑1 (MQ=255)
                                                        tgactttcGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.GAA.ACCCATCCC.TTTCATC.TTG..CCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGc.ccccc                                                 <  2:5759785/94‑2 (MQ=25)
                                                           ctttcGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.GAA.ACCCATCCC.TTTCATC.TTG..CCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGc.cccccgcc                                              <  1:5462351/97‑5 (MQ=25)
                                                           ctttcGATGCCTCCGGC.GGGGATATTNGNGNCAAGAT.GAA.ACCCATCCC.TTTCATC.TTG..CCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGc.cccccgcc                                              <  1:4585024/97‑5 (MQ=21)
                                                           cGGCCGATGCCTCCGGC.GGGGATATTTTGGGGAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..TCCCAAATATCCCCGGGGTGC..GGGGGCCGAGC.CCCcgccgc                                             >  2:1678654/1‑98 (MQ=37)
                                                            tttcGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.GAA.ACCCATCCC.TTTCATC.TTG..CCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGc.cccccgcca                                             <  1:3044469/98‑6 (MQ=25)
                                                            ggCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGTGCAGAGC.CCCcgccgcc                                            <  2:5988698/101‑5 (MQ=255)
                                                             ttcGATGCCTCCGGC.GGGGATATTTTGGGCAAGAT.GAA.ACCCATCCC.TTTCATC.TTT..CCCCCAATATCCCGGGGGGCC..CGGGGGGGAGCGCCCCCcgcccc                                           >  1:4343352/3‑99 (MQ=21)
                                                               cGATGCCTCCGGC.GGGGATATTTTGGGCAAGAT.GAA.AACCCTCCC.TTTTCTC.TTG..CCCCAAAAATCCCGGGGGGCC..CGGGGGGGAGc.gcccccccccccct                                        >  2:4841360/1‑91 (MQ=18)
                                                                 aTGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACggg                                     >  1:387912/1‑100 (MQ=255)
                                                                  tGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGGGC..GGGGGCAGAGG.CCC..CGCCGCCACGGtt                                    >  2:2214242/1‑101 (MQ=18)
                                                                     ccccggggggggTTGTTGGGGACAAGAT.AAGGGGGTTTTTT.TTTTTTC..TT..CCCCAAAT.TACCGGGGGGGG..GGGGGGGCAGC.CCCCCCGCCGCCACGGTTCa                                  <  2:6556221/93‑1 (MQ=255)
                                                                       gcgcggggggGTATTTTGGGGAAAAT.ATAGGGGGGTTTT.TTTTTTT.TCT..TCCCAAAAATCGCGGGGGGGG..GGGGGGGCAGC.CCCCCCGCCGCCACGGTTCa                                  <  2:3025894/95‑1 (MQ=255)
                                                                         ggc.ggGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCAcgccg                             >  2:3773995/1‑101 (MQ=255)
                                                                          gggggggGTTTTGGGGGAAAAAA.AAAGGGTGTTTTT.TTTTTTT.CTC..CCCCAAATTCCCCGGGGGGGG..GGGGGC.CACC.CCCCCCGCCGCCACGGTTCAcgcc                              <  1:2099666/98‑1 (MQ=255)
                                                                           c.ggGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCcgcg                           >  1:271182/1‑101 (MQ=255)
                                                                             ggggATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGCTCACGCCGCGa                          >  2:1777576/1‑101 (MQ=255)
                                                                              gggATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGAc                         >  1:4134508/1‑101 (MQ=255)
                                                                               ggATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGAcc                        >  2:4777677/1‑101 (MQ=255)
                                                                                gATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCg                       >  2:3332655/1‑101 (MQ=255)
                                                                                gATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGCTCACGCCGCGACCg                       >  2:439511/1‑101 (MQ=255)
                                                                                  tatTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGtc                     >  2:7077351/1‑101 (MQ=255)
                                                                                  tatTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGtc                     >  1:585913/1‑101 (MQ=255)
                                                                                   atTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGtct                    >  2:1452312/1‑101 (MQ=255)
                                                                                      tGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                 >  2:2804644/1‑101 (MQ=255)
                                                                                      tGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                 >  1:799538/1‑101 (MQ=255)
                                                                                            aaGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg           >  1:350862/1‑101 (MQ=255)
                                                                                             aGAT.AAAGGGTCTTTGC.TTTTATC.TTC..CCCAAAATACCCCGGGGGTGC..GGGGGCAGA.C.CCCC.CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGc          <  1:4405892/101‑1 (MQ=25)
                                                                                             aGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGc          >  1:2785758/1‑101 (MQ=255)
                                                                                              gAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc         >  2:1122311/1‑101 (MQ=255)
                                                                                              gAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc         >  1:1366587/1‑101 (MQ=255)
                                                                                               aT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa        >  1:1536312/1‑101 (MQ=255)
                                                                                               aT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa        >  2:2121171/1‑101 (MQ=255)
                                                                                               aT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa        >  1:4204189/1‑101 (MQ=255)
                                                                                               aT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa        >  2:2738773/1‑101 (MQ=255)
                                                                                                t.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg       >  1:628374/1‑101 (MQ=255)
                                                                                                t.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg       >  2:28872/1‑101 (MQ=255)
                                                                                                  aaaGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc      >  1:250897/1‑101 (MQ=255)
                                                                                                    aGGTGCTTTGC.TTTTATC.TTT..CCCCAAATATCCGGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc    >  1:5020564/1‑101 (MQ=37)
                                                                                                    aGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc    >  1:1880328/1‑101 (MQ=255)
                                                                                                      gggCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcga  >  2:681900/1‑101 (MQ=255)
                                                                                                      |                                                                                                             
GCTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.TTT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGC.CCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGA  >  NC_014034/3439692‑3439894

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG