Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,794 G→C 6.4% intergenic (‑97/+66) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,7940G→C 6.4% 22.6 ‑1.8 ‑0.0217intergenic (‑97/+66)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (5/9):  ref base (114/89):  total (119/98)
Fisher's exact test strand distribution p-value = 1.69e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.97e-01

Only 100 of 310 total aligned reads displayed.

CATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGA  >  NC_014034/3439695‑3439894
                                                                                                     |                                                                                                         
cATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGgc                                                                                                          <  2:574303/101‑1 (MQ=255)
 aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGCGATTGGGGGCAA.AT.AAAGGGgct                                                                                                         >  1:1067935/1‑101 (MQ=255)
 aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTTGGGGGAGAG.AAAgggggt                                                                                                         >  1:6548991/1‑99 (MQ=255)
   ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGgcttt                                                                                                       <  2:5284846/101‑1 (MQ=255)
    gcggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGgctttg                                                                                                      <  2:2692575/101‑1 (MQ=255)
     cggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCCAGGT.AAAGGGggtttgc                                                                                                     >  1:3806375/1‑101 (MQ=255)
      ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGGCAGAT.AAAGGGgctttgc.t                                                                                                   >  2:6385392/1‑101 (MQ=255)
      ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGAATTTGGGGAAGAA.AAAAGGgggttgc.t                                                                                                   >  2:6232889/1‑101 (MQ=255)
       gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGgctttgc.tt                                                                                                  <  1:2723337/101‑1 (MQ=255)
       gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGgctttgc.tt                                                                                                  <  1:298368/101‑1 (MQ=255)
       gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGgctttgc.tt                                                                                                  <  2:1039143/101‑1 (MQ=255)
       gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGgctttgc.tt                                                                                                  <  2:2128981/101‑1 (MQ=255)
         gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTGGGGGGAGAG.AAAAGGGGTTTGC.tttt                                                                                                >  2:4502665/1‑101 (MQ=255)
         gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGGAAGAT.AAAGGGGCTTTTC.tttt                                                                                                >  1:886000/1‑101 (MQ=255)
           cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTTGGGCAAGATAAAAGGGGGTTTGT.ttttt                                                                                               >  1:1618485/1‑100 (MQ=255)
           cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTAt                                                                                              <  1:7036369/101‑1 (MQ=255)
           cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTAt                                                                                              <  1:4102175/101‑1 (MQ=255)
           accTGCACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTAt                                                                                              <  2:1858758/100‑1 (MQ=255)
            ccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGGAAGAT.AAAAGGGGTTTGG.ttttttt                                                                                             >  1:3056068/1‑98 (MQ=255)
             cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTGGGGCAAGAG.AAAGGGGGTTTGC.TTTTAttt                                                                                            >  1:1909414/1‑99 (MQ=39)
             cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTagat                                                                                            >  1:116295/1‑98 (MQ=255)
              tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGGCAGAT.AAAAGGGCTTTGC.TTTTTTC.t.t                                                                                         >  1:4440987/1‑101 (MQ=255)
               acacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTGGGGGAAGAT.AAAAGGGGGTTGC.TTTTTTT.t.tt                                                                                        >  1:6348841/1‑101 (MQ=255)
               acacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.t.tt                                                                                        <  2:1168681/101‑1 (MQ=255)
                 ccGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTcc                                                                                      <  2:5435566/100‑1 (MQ=255)
                 acGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTcc                                                                                      <  2:3069714/101‑1 (MQ=255)
                   ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTcccc                                                                                    <  2:3505286/101‑1 (MQ=255)
                   ggCGCCACCAGCTTTCGAAATACAGGAATTCTGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTcccc                                                                                    <  1:2271509/101‑1 (MQ=255)
                   ggCGCAACCAGCTTGCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTcccc                                                                                    <  2:2609785/101‑1 (MQ=255)
                      gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCaaa                                                                                 <  1:372520/101‑1 (MQ=255)
                       cgACCAGCTTTCGAAAGACATGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAat                                                                                <  1:4359064/101‑1 (MQ=255)
                       cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAat                                                                                <  1:1796883/101‑1 (MQ=255)
                       cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAat                                                                                <  2:4677933/101‑1 (MQ=255)
                        gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGGAAGAT.AAAAGGGCTTTGC.TTTTTTCTT.TTCCCCaaaa                                                                                >  1:5292946/1‑100 (MQ=38)
                        gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAata                                                                               <  2:4037015/101‑1 (MQ=255)
                         aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAatat                                                                              <  1:3879407/101‑1 (MQ=255)
                           cAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGCAAGAT.AAAGGGGCGTTGC.TTTTTTT.T.TTTCCCAAATATcc                                                                            >  1:2877760/1‑101 (MQ=38)
                               tttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGGAAGAT.AAAGGGGGGTTTC.TTTTTTT.T.TTCCCCAAAAATTCCggg                                                                        >  2:5150565/1‑101 (MQ=37)
                                ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGGAAGAT.AAAGGGGGTTTGC.TTTTTTT.T.TTTCCCCAAAATCCCgggg                                                                       >  1:6121268/1‑101 (MQ=255)
                                ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCgggg                                                                       <  1:5534737/101‑1 (MQ=255)
                                ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCgggg                                                                       <  2:2904432/101‑1 (MQ=255)
                                 tCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGGAAGAT.AAAAGGGGGTTGC.TTTTATT.T.TTTCCCCAATAAACCggggg                                                                      >  1:5988167/1‑101 (MQ=255)
                                  cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGGAAGAT.AAAGGGGCTTTGC.TTTTTTT.T.TTTCCCAAATATCCCgggggg                                                                     >  1:6708010/1‑100 (MQ=255)
                                  cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGGTTTGC.TTTTATC.T.TTTCCCCAATATCCCgggggg                                                                     >  2:4454851/1‑100 (MQ=255)
                                    aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGc                                                                   <  2:6981681/101‑1 (MQ=255)
                                    aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGc                                                                   <  2:4368925/101‑1 (MQ=255)
                                    aaaGACAGGAATTCGGCCTGAGGCCGATGCCTCCGGC.GGGGGTATTTGGGGCAAGAT.AAAAGGGGTTTGCTTTTTTTT.T.TTCCCCAAAAATCCGggggggg                                                                    >  1:6384628/1‑99 (MQ=25)
                                      aGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGAATTTGGGGGAAAAT.AAAGGGGCTTTGG.TTTTTTC.T.TTCCCCCCAAATCCCgggggggggg                                                                 >  1:1255659/1‑96 (MQ=255)
                                      aGACAGGAATTCGGCCTGCGGCCCATGCCTCCGGC.GGGCGTATTTGGGGCGAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCTCCACAAATACCTGCGGTGC..gg                                                               >  1:5876062/1‑101 (MQ=255)
                                        aCAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTTTC.T.TTCCCCCAATATCCCGGGGGTGC..gggg                                                             >  2:3646884/1‑101 (MQ=255)
                                          aGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTTTC.T.TTCCCCCAATATCCCCGGGGGGC..GGGGGc                                                           >  1:1124096/1‑101 (MQ=37)
                                          aGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCATATATCCCGGGGGTGC..GGGGGc                                                           >  2:1546605/1‑101 (MQ=255)
                                          aGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGc                                                           <  2:4240369/101‑1 (MQ=255)
                                          aGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGc                                                           <  1:6233208/101‑1 (MQ=255)
                                          aGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGc                                                           <  1:3184716/101‑1 (MQ=255)
                                           ggAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGCAAGAT.AAAGGGGGTTTGC.TTTTATC.T.TTCCCCCAATATCCCGGGGGGGG..ggggggg                                                          >  1:679386/1‑99 (MQ=37)
                                            gAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGGAAGAT.AAAGGGGGTTTGC.TTTTATC.T.TTTCCCCAATATCCCCGGGGTGC..GGGGGGag                                                         >  2:5927015/1‑101 (MQ=255)
                                             aaTTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCaga                                                        <  1:2122945/101‑1 (MQ=255)
                                                tCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGGAAGAT.AAAGGGGCTTTGC.TTTTTTC.T.TTCCCCAAATATCCCGGGGGGGC..GGGGGCagaggc                                                     >  2:4142159/1‑99 (MQ=38)
                                                 cGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTTGGGGAAGAT.AAAGGGGGTTTGC.TTTTATC.T.TTCCCCAAATATTCCGGGGGGGC..GGGGGCAGAGgcc                                                    >  2:645105/1‑101 (MQ=37)
                                                 cGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTTGGGGAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGccc                                                    >  2:76352/1‑101 (MQ=255)
                                                  ggCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGCAAGAT.AAAGGGGCTTTGC.TTTTTTC.T.TTTCCCAAATATCCCCGGGGGGC..GGGGGGCGAGcccc                                                   >  1:2601482/1‑101 (MQ=25)
                                                  ggCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGcccc                                                   <  2:1296618/101‑1 (MQ=255)
                                                   gCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGccccc                                                  <  1:4556567/101‑1 (MQ=255)
                                                    ccTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCcg                                                 >  1:3284537/1‑101 (MQ=255)
                                                    ccTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCcg                                                 <  1:3106942/101‑1 (MQ=255)
                                                        ctttcGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.GAA.ACCCATCCC.TTTCATC.T.TGCCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGccccccgcc                                              <  1:5462351/97‑5 (MQ=25)
                                                        ctttcGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.GAA.ACCCATCCC.TTTCATC.T.TGCCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGccccccgcc                                              <  1:2045940/97‑5 (MQ=25)
                                                        cGGCCGATGCCTCCGGC.GGGGATATTTGGGGCCAGAT.AAAGGGGCTTTGG.TTTTATC.T.TTTCCCCAATATCCCGGGGGTGG..GGGGGCCGAGGCCCcgccgc                                             >  2:1787442/1‑98 (MQ=37)
                                                            cGATGCCTCCGGC.GGGGATATTTTGGGCAAGAT.GAA.AACCCTCCC.TTTTCTC.T.TGCCCCAAAAATCCCGGGGGGCC..CGGGGGGGAGcgcccccccccccct                                        >  2:4841360/1‑91 (MQ=18)
                                                               tGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGGGC..GGGGGCAGAGGCCC..CGCCGCCACGGtt                                    >  2:2214242/1‑101 (MQ=18)
                                                                  ccccggggggggTTGTTGGGGACAAGAT.AAGGGGGTTTTTT.TTTTTTC...TTCCCCAAAT.TACCGGGGGGGG..GGGGGGGCAGCCCCCCCGCCGCCACGGTTCa                                  <  2:6556221/93‑1 (MQ=255)
                                                                     cggggggggTTTTTTGGGGAAAGAA.AAAGGGG.TTTTT.TTTTTTC.TCTTCCCCAAA.ATCCCCGGGGGGG..GGGGGGGCAGCCCCCCCGCCGCCACGGTTCAcg                                <  2:1708078/96‑1 (MQ=255)
                                                                      ggc.ggGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCAcgccg                             >  2:3773995/1‑101 (MQ=255)
                                                                        c.ggGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCcgcg                           >  1:271182/1‑101 (MQ=255)
                                                                           gggATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAc                         >  1:4134508/1‑101 (MQ=255)
                                                                            ggATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                        >  2:4777677/1‑101 (MQ=255)
                                                                             gATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCg                       >  2:3332655/1‑101 (MQ=255)
                                                                               tatTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtc                     >  2:7077351/1‑101 (MQ=255)
                                                                                atTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                    >  2:1452312/1‑101 (MQ=255)
                                                                                   tGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                 >  1:799538/1‑101 (MQ=255)
                                                                                   tGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                 >  2:2804644/1‑101 (MQ=255)
                                                                                   tGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCCTCTCCg                 >  2:1943699/1‑101 (MQ=255)
                                                                                        cAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc            >  1:255660/1‑101 (MQ=255)
                                                                                        cAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc            >  1:4291677/1‑101 (MQ=255)
                                                                                         aaGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg           >  1:350862/1‑101 (MQ=255)
                                                                                         aaGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg           >  2:5572224/1‑101 (MQ=255)
                                                                                          aGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGc          >  1:2785758/1‑101 (MQ=255)
                                                                                           gAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc         >  1:1366587/1‑101 (MQ=255)
                                                                                           gAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc         >  2:1122311/1‑101 (MQ=255)
                                                                                            aT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa        >  1:4796971/1‑101 (MQ=255)
                                                                                            aT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa        >  2:2738773/1‑101 (MQ=255)
                                                                                            aT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa        >  1:1536312/1‑101 (MQ=255)
                                                                                             t.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg       >  1:628374/1‑101 (MQ=255)
                                                                                                aaGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg     >  2:6022482/1‑101 (MQ=255)
                                                                                                aaGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg     >  2:1428373/1‑101 (MQ=255)
                                                                                                 aGGTGCTTTGC.TTTTATC.T.TTCCCCAAATATCCGGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc    >  1:5020564/1‑101 (MQ=37)
                                                                                                 aGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc    >  1:1880328/1‑101 (MQ=255)
                                                                                                  ggggCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGcgcg   >  1:4621381/1‑101 (MQ=255)
                                                                                                   gggCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcga  >  2:681900/1‑101 (MQ=255)
                                                                                                     |                                                                                                         
CATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGC.TTTTATC.T.TTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGA  >  NC_014034/3439695‑3439894

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG