Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,796 T→A 6.1% intergenic (‑99/+64) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,7960T→A 6.1% 23.8 ‑1.8 ‑0.0219intergenic (‑99/+64)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (5/9):  ref base (116/89):  total (121/98)
Fisher's exact test strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.40e-01

Only 100 of 311 total aligned reads displayed.

ATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGAT  >  NC_014034/3439696‑3439898
                                                                                                      |                                                                                                          
aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCGGGC.GGGGATATTTGGGGCAAGAT.AAAGGGgct                                                                                                            <  2:2189931/101‑1 (MQ=255)
aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGCGATTGGGGGCAA.AT.AAAGGGgct                                                                                                            >  1:1067935/1‑101 (MQ=255)
aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCCAGAT.AAAGGGgct                                                                                                            >  2:1796819/1‑101 (MQ=255)
  ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGgcttt                                                                                                          <  2:5284846/101‑1 (MQ=255)
   gcggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGgctttg                                                                                                         <  2:2692575/101‑1 (MQ=255)
     ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGGCAGAT.AAAGGGgctttgct                                                                                                       >  2:6385392/1‑101 (MQ=255)
     ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGAATTTGGGGAAGAA.AAAAGGgggttgct                                                                                                       >  2:6232889/1‑101 (MQ=255)
     ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGgctttgct                                                                                                       <  1:6255691/101‑1 (MQ=255)
     ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGgctttgct                                                                                                       >  2:1150217/1‑101 (MQ=255)
      gcgACCCTCCACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGgctttgctt                                                                                                      <  2:5663782/101‑1 (MQ=255)
       cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGgctttgcttt                                                                                                     <  1:4755182/101‑1 (MQ=255)
       cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGgctttgcttt                                                                                                     <  1:3860973/101‑1 (MQ=255)
        gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCtttt                                                                                                    <  2:1441188/101‑1 (MQ=255)
         aCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTa                                                                                                   <  1:6135412/101‑1 (MQ=255)
          cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTTGGGCAAGATAAAAGGGGGTTTGTttttt                                                                                                   >  1:1618485/1‑100 (MQ=255)
          cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTAt                                                                                                  <  1:873667/101‑1 (MQ=255)
            cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTagat                                                                                                >  1:116295/1‑98 (MQ=255)
             tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTTGGGGAAGAT.AAAGGGGCTTTGGTCTTTTCtt                                                                                               >  2:1872818/1‑101 (MQ=38)
             tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTaagat                                                                                               >  2:1624733/1‑97 (MQ=255)
             tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCtt                                                                                               <  2:284292/101‑1 (MQ=255)
                acGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGGAAGAA.AAAAGGGGTTTTCTTTTTTTttttc                                                                                            >  1:2716896/1‑99 (MQ=37)
                 cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.ccc                                                                                          <  1:316649/101‑1 (MQ=255)
                  ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.cccc                                                                                         <  2:3505286/101‑1 (MQ=255)
                  ggCGCCACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.cccc                                                                                         <  1:2271658/101‑1 (MQ=255)
                  ggCGCAACCAGCTTGCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.cccc                                                                                         <  2:2609785/101‑1 (MQ=255)
                     gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCaaa                                                                                      <  1:5407918/101‑1 (MQ=255)
                      cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAat                                                                                     <  2:4677933/101‑1 (MQ=255)
                      cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAat                                                                                     <  1:2089794/101‑1 (MQ=255)
                      cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAat                                                                                     <  1:1796883/101‑1 (MQ=255)
                      cgACCAGCCTTCGAAAGACACGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAat                                                                                     <  1:4381797/101‑1 (MQ=255)
                       gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGGCAGATAAAAGGGGTTTTGTTTTTTTTTTT.CCCCaaaa                                                                                     >  1:418460/1‑100 (MQ=255)
                       gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTGGGGGAAGAT.AAAGGGGGGTTGGTTTTATCTTT.CCCCacaaa                                                                                    >  1:6352635/1‑97 (MQ=255)
                       gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAata                                                                                    <  2:7098901/101‑1 (MQ=255)
                       gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAata                                                                                    <  2:4037015/101‑1 (MQ=255)
                        aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAatat                                                                                   <  1:3729819/101‑1 (MQ=255)
                         ccAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.c                                                                                 <  1:5117395/101‑1 (MQ=255)
                          cAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGCAAGAT.AAAGGGGGTTTGCTTTTTTTTTT.TCCCCAATAT.cc                                                                                >  1:1892807/1‑101 (MQ=38)
                              tttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGGAAGAT.AAAGGGGGGTTTCTTTTTTTTTT.CCCCAAAAAT.TCCggg                                                                            >  2:5150565/1‑101 (MQ=37)
                              tttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGGTTTGCTTTTATCTTT.CCCCACATAT.CCCggg                                                                            >  1:718392/1‑101 (MQ=255)
                                 cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGGAAGAT.AAAGGGGCTTTGCTTTTTTTTTT.TCCCAAATAT.CCCgggggg                                                                         >  1:6708010/1‑100 (MQ=255)
                                  gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGCAAGAT.AAAGGGGGTTTGCTTTTATTTTT.TCCCCAATAT.CCCggggggg                                                                        >  1:6885547/1‑99 (MQ=38)
                                  gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTg                                                                        <  1:4840420/101‑1 (MQ=255)
                                   aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGc                                                                       <  2:4368925/101‑1 (MQ=255)
                                     aGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGAATTTGGGGGAAAAT.AAAGGGGCTTTGGTTTTTTCTTT.CCCCCCAAAT.CCCgggggggggg                                                                     >  1:1255659/1‑96 (MQ=255)
                                       aCAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTTTCTTT.CCCCCAATAT.CCCGGGGGTGC..gggg                                                                 >  2:3646884/1‑101 (MQ=255)
                                        cAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..ggggg                                                                <  2:2064093/101‑1 (MQ=255)
                                         aGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTTTCTTT.CCCCCAATAT.CCCCGGGGGGC..GGGGGc                                                               >  1:1124096/1‑101 (MQ=37)
                                         aGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGc                                                               <  2:4240369/101‑1 (MQ=255)
                                          ggAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGCAAGAT.AAAGGGGGTTTGCTTTTATCTTT.CCCCCAATAT.CCCGGGGGGGG..ggggggg                                                              >  1:679386/1‑99 (MQ=37)
                                           gAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGGAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCCAATAT.CCCGGGGGTGC..GGGGGGag                                                             >  2:7004615/1‑101 (MQ=37)
                                               tCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGGAAGAT.AAAGGGGCTTTGCTTTTTTCTTT.CCCCAAATAT.CCCGGGGGGGC..GGGGGCagaggc                                                         >  2:4142159/1‑99 (MQ=38)
                                               tCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGGAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCCAATAT.CCCCGGGGGGC..GGGGGCGGGGcc                                                         >  1:1944941/1‑101 (MQ=37)
                                                cGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTTGGGGAAGAT.AAAGGGGGTTTGCTTTTATCTTT.CCCCAAATAT.TCCGGGGGGGC..GGGGGCAGAGgcc                                                        >  2:645105/1‑101 (MQ=37)
                                                cGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTTGGGGAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGccc                                                        >  2:76352/1‑101 (MQ=255)
                                                cGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.TCCCCAAAAT.CCCGGGGGGGG..GGGGGGAGGGccc                                                        >  1:3652114/1‑101 (MQ=25)
                                                cGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGccc                                                        <  1:259569/101‑1 (MQ=255)
                                                 ggCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGcccc                                                       >  1:990034/1‑101 (MQ=255)
                                                  gCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGccccc                                                      <  2:1481812/101‑1 (MQ=255)
                                                  gCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGccccc                                                      <  1:2764485/101‑1 (MQ=255)
                                                  gCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGccccc                                                      <  2:1067900/101‑1 (MQ=255)
                                                   gtgactttcGATGCCTCCGGC.GGGGGTATTTTGGGCAAGAT.GAA.ACCCATCCCCTTCATCTTT.CCCCCAAAAT.CCCGGGGGGCCCGGGGGGCAGCGccccc                                                      >  1:2695008/9‑101 (MQ=25)
                                                   ccTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGGCAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCCGGGGTGC..GGGGGCAGAGcccccc                                                     >  2:3131638/1‑100 (MQ=255)
                                                   ccTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCCcg                                                     <  2:1258793/101‑1 (MQ=255)
                                                    tgcctttcGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.GAA.ACCCATCCCTTTCATCTTG.CCCCAAATAT.CCCGGGGGGCCCGGGGGGCAGCGcccccc                                                     <  2:5429840/94‑2 (MQ=25)
                                                    tgactttcGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.GAA.ACCCATCCCTTTCATCTTG.CCCCAAATAT.CCCGGGGGGCCCGGGGGGCAGCGcccccc                                                     <  1:2135005/94‑2 (MQ=25)
                                                      actttcGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.GAA.ACCCATCCCTTTCATCTTG.CCCCAAATAT.CCCGGGGGGCCCGGGGGGCAGCGccccccgc                                                   <  1:6051350/96‑4 (MQ=25)
                                                        tttcGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.GAA.AACCATCCCTTTCATCTTG.CCCCAAATATCCCCGGGGGCCCG.GGGGGCAGCGccccccgccc                                                 >  2:5496352/4‑96 (MQ=21)
                                                        ggCCGATGCCTCCGGC.GGGGATATTTTGGGGAAGAT.AAAGGGGCTTTGCTTTTTTCTTT.CCCCCAATAT.CCCGGGGGGGC..GGGGGCAGAGccccccccgcc                                                >  1:5891818/1‑95 (MQ=21)
                                                        ggCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGTT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGGGC..GGGGGCAGAGCCCCcgccgcc                                                >  1:4130178/1‑97 (MQ=255)
                                                         gCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGGGC..GGGGGCAGAGCCCCcgccgcca                                               >  1:3075811/1‑96 (MQ=255)
                                                          ccGATGCCTCCGGC.GGGGATATTTGGGGGAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGGGC..GGGGGCAGAGCCCCcgccgcccc                                              >  1:2932390/1‑95 (MQ=21)
                                                          ccGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCCcgccgccac                                              >  2:2957496/1‑95 (MQ=255)
                                                           cGATGCCTCCGGC.GGGGATATTTTGGGCAAGAT.GAA.AACCCTCCCTTTTCTCTTG.CCCCAAAAAT.CCCGGGGGGCC..CGGGGGGGAGcgcccccccccccct                                            >  2:4841360/1‑91 (MQ=18)
                                                                 ccccggggggggTTGTTGGGGACAAGAT.AAGGGGGTTTTTTTTTTTTC.TT.CCCCAAAT.T.ACCGGGGGGGG..GGGGGGGCAGCCCCCCCGCCGCCACGGTTCa                                      <  2:6556221/93‑1 (MQ=255)
                                                                  tCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGGGC..GGGGGCAGAGCCCC..CGCCGCCACGGGTCAcc                                    >  2:2443791/1‑100 (MQ=18)
                                                                   ccGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGGTCAcgc                                   >  2:5837980/1‑101 (MQ=255)
                                                                     ggc.ggGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCAcgccg                                 >  2:3773995/1‑101 (MQ=255)
                                                                     ggc.ggGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGGGC..GGGGGCAGAGCCCC..CGCCGCCACGGGTCAccccg                                 >  1:5523315/1‑101 (MQ=18)
                                                                         ggggATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                              >  2:1709528/1‑101 (MQ=255)
                                                                           ggATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                            >  2:4777677/1‑101 (MQ=255)
                                                                             atatTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.ACCCAAATAT.CCCGGGGGAGG..GGGGGAAGGGNNCC..NGCCGCCAAGGTTCCCGCAGCGACCgc                          >  1:3977015/1‑100 (MQ=18)
                                                                                 ttGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTcc                      >  1:5772179/1‑101 (MQ=255)
                                                                                  tGGGGCAAGAT.ACAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                     >  1:6318477/1‑101 (MQ=255)
                                                                                  tGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                     >  2:4477372/1‑101 (MQ=255)
                                                                                       cAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc                >  2:279199/1‑101 (MQ=255)
                                                                                       cAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc                >  1:3040399/1‑101 (MQ=255)
                                                                                        aaGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg               >  1:4579647/1‑101 (MQ=255)
                                                                                          gAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc             >  1:3263183/1‑101 (MQ=255)
                                                                                          gAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc             >  1:1366587/1‑101 (MQ=255)
                                                                                           aT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa            >  2:2738773/1‑101 (MQ=255)
                                                                                           aT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa            >  1:1536312/1‑101 (MQ=255)
                                                                                              aagggggTTTTTTTTTTTTCTCTCCCCCAAATTC.CCCGGGGGGGG..GGGGGGCG.GCCCCCCCCCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa            <  1:4455073/98‑1 (MQ=255)
                                                                                              aaaGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc          >  1:3318609/1‑101 (MQ=255)
                                                                                              aaaGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGGCGCCAgc          >  1:6449584/1‑101 (MQ=255)
                                                                                               aaGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg         >  2:6022482/1‑101 (MQ=255)
                                                                                                aGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc        >  1:4331871/1‑101 (MQ=255)
                                                                                                aGGGGCTCTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc        >  2:443185/1‑101 (MQ=255)
                                                                                                  gggCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcga      >  2:681900/1‑101 (MQ=255)
                                                                                                     ctttgctttTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgacga   >  2:1426236/1‑101 (MQ=255)
                                                                                                     ctttgctttTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgacga   >  1:285474/1‑101 (MQ=255)
                                                                                                      |                                                                                                          
ATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGAT  >  NC_014034/3439696‑3439898

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG