Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,798 T→C 6.1% intergenic (‑101/+62) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,7980T→C 6.1% 18.8 ‑1.8 0.0219intergenic (‑101/+62)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (5/9):  ref base (116/89):  total (121/98)
Fisher's exact test strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 309 total aligned reads displayed.

GGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATAT  >  NC_014034/3439698‑3439900
                                                                                                    |                                                                                                           
ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGCCAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGgcttt                                                                                                             <  1:6415768/101‑1 (MQ=255)
ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGgcttt                                                                                                             <  2:5284846/101‑1 (MQ=255)
 gcggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGgctttg                                                                                                            <  2:2692575/101‑1 (MQ=255)
   ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGGCAGATAAAGGGgctttgct                                                                                                          >  2:6385392/1‑101 (MQ=255)
   ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGAATTTGGGGAAGAAAAAAGGgggttgct                                                                                                          >  2:6232889/1‑101 (MQ=255)
   ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGgctttgct                                                                                                          <  1:6602745/101‑1 (MQ=255)
    gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGgctttgctt                                                                                                         <  2:1039143/101‑1 (MQ=255)
    gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGgctttgctt                                                                                                         <  2:2128981/101‑1 (MQ=255)
    gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGgctttgctt                                                                                                         <  1:2723337/101‑1 (MQ=255)
     cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGgctttgcttt                                                                                                        <  1:1870942/101‑1 (MQ=255)
     cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGgctttgcttt                                                                                                        <  1:4755182/101‑1 (MQ=255)
     cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGgctttgcttt                                                                                                        <  1:3860973/101‑1 (MQ=255)
      gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGGAAGATAAAGGGGCTTTTCtttt                                                                                                       >  1:886000/1‑101 (MQ=255)
      gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCtttt                                                                                                       <  1:19595/101‑1 (MQ=255)
       atcttACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTa                                                                                                      <  1:1624794/97‑1 (MQ=255)
        cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTAt                                                                                                     <  1:7036369/101‑1 (MQ=255)
        accTGCACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTAt                                                                                                     <  2:1858758/100‑1 (MQ=255)
         ccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGATAAAAGGGGTTTGGttttttt                                                                                                    >  1:3056068/1‑98 (MQ=255)
         ccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATc                                                                                                    <  1:2991409/101‑1 (MQ=255)
          cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGGAGATAAAGGGGGTTTGCtttttttt                                                                                                   >  2:259565/1‑97 (MQ=255)
          cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCt                                                                                                   <  2:3610812/101‑1 (MQ=255)
           tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGCAGATAAAAGGGCTTTGCTTTTTTCt.t                                                                                                 >  1:4440987/1‑101 (MQ=255)
           tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCt.t                                                                                                 <  2:284292/101‑1 (MQ=255)
            acacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCt.tt                                                                                                <  2:1168681/101‑1 (MQ=255)
            acacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCt.tt                                                                                                <  1:1274437/101‑1 (MQ=255)
                ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.cccc                                                                                           <  1:5483628/101‑1 (MQ=255)
                 gcgcGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCa                                                                                          <  1:1379627/101‑1 (MQ=255)
                   gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCaaa                                                                                        <  1:372520/101‑1 (MQ=255)
                    cgACCAGCTTTCGAAAGACATGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAat                                                                                       <  1:4359064/101‑1 (MQ=255)
                    cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAat                                                                                       <  1:2089794/101‑1 (MQ=255)
                     gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTGGGGGAAGATAAAGGGGGGTTGGTTTTATCT.TT.CCCCacaaa                                                                                      >  1:6352635/1‑97 (MQ=255)
                     gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAata                                                                                      <  2:7098901/101‑1 (MQ=255)
                       ccAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.c                                                                                   <  1:5117395/101‑1 (MQ=255)
                        cAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.cc                                                                                  <  2:4618377/101‑1 (MQ=255)
                           cTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGGAAGATAAAGGGGGTTTGCTTTTTTCT.TT.CCCCCAAAAT.CCCgg                                                                               >  1:4127071/1‑101 (MQ=38)
                            tttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGGAAGATAAAGGGGGGTTTCTTTTTTTT.TT.CCCCAAAAAT.TCCggg                                                                              >  2:5150565/1‑101 (MQ=37)
                             ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGATAAAGGGGGTTTGCTTTTTTTT.TT.TCCCCAAAAT.CCCgggg                                                                             >  1:6121268/1‑101 (MQ=255)
                             ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGCCAGATAAAAGGGCTTTGGTTTTTTTT.TT.TCCCCAATAT.TCCcggg                                                                             >  1:1097473/1‑101 (MQ=255)
                              tCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGATAAAAGGGGGTTGCTTTTATTT.TT.TCCCCAATAA.ACCggggg                                                                            >  1:5988167/1‑101 (MQ=255)
                                gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTg                                                                          <  1:4840420/101‑1 (MQ=255)
                                 aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGGAAGATAAAAGGGGTTTGCTTTTTTCT.TT.CCCCAAATAT.CCCGGGGGGGc                                                                         >  1:1536962/1‑101 (MQ=37)
                                 aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGc                                                                         <  2:4368925/101‑1 (MQ=255)
                                   aGACAGGAATTCGGCCTGCGGCCCATGCCTCCGGCGGGCGTATTTGGGGCGAGATAAAGGGGCTTTGCTTTTATCT.TT.CTCCACAAAT.ACCTGCGGTGC..gg                                                                     >  1:5876062/1‑101 (MQ=255)
                                    gACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..ggg                                                                    <  2:3435200/101‑1 (MQ=255)
                                       aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGc                                                                 <  1:6233208/101‑1 (MQ=255)
                                       aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGc                                                                 <  2:4240369/101‑1 (MQ=255)
                                       aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGc                                                                 <  1:3184716/101‑1 (MQ=255)
                                         gAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGATAAAGGGGGTTTGCTTTTATCT.TT.TCCCCAATAT.CCCCGGGGTGC..GGGGGGag                                                               >  2:5927015/1‑101 (MQ=255)
                                         gAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCCAATAT.CCCGGGGGTGC..GGGGGGag                                                               >  2:7004615/1‑101 (MQ=37)
                                         gAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCCAATAT.CCCGGGGGGGC..GGGGGGag                                                               >  1:5348686/1‑101 (MQ=37)
                                              cGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTTGGGGAAGATAAAGGGGGTTTGCTTTTATCT.TT.CCCCAAATAT.TCCGGGGGGGC..GGGGGCAGAGgcc                                                          >  2:645105/1‑101 (MQ=37)
                                              cGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTTGGGGAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGccc                                                          >  2:76352/1‑101 (MQ=255)
                                               ggCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGCAAGATAAAGGGGCTTTGCTTTTTTCT.TT.TCCCAAATAT.CCCCGGGGGGC..GGGGGGCGAGcccc                                                         >  1:2601482/1‑101 (MQ=25)
                                               ggCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGcccc                                                         <  1:6850273/101‑1 (MQ=255)
                                                gCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGccccc                                                        <  1:4556567/101‑1 (MQ=255)
                                                 ccTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGGCAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCCGGGGTGC..GGGGGCAGAGcccccc                                                       >  2:3131638/1‑100 (MQ=255)
                                                 ccTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCCcg                                                       >  1:3284537/1‑101 (MQ=255)
                                                 ccTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCCcg                                                       <  1:3106942/101‑1 (MQ=255)
                                                  tgcctttcGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAA.ACCCATCCCTTTCATCT.TG.CCCCAAATAT.CCCGGGGGGCCCGGGGGGCAGCGcccccc                                                       <  2:5429840/94‑2 (MQ=25)
                                                  tgactttcGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAA.ACCCATCCCTTTCATCT.TG.CCCCAAATAT.CCCGGGGGGCCCGGGGGGCAGCGcccccc                                                       <  2:5759785/94‑2 (MQ=25)
                                                  tgactttcGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAA.ACCCATCCCTTTCATCT.TG.CCCCAAATAT.CCCGGGGGGCCCGGGGGGCAGCGcccccc                                                       <  1:2135005/94‑2 (MQ=25)
                                                  cTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCCcgc                                                      >  2:4124060/1‑101 (MQ=255)
                                                     cGGCCGATGCCTCCGGCGGGGATATTTGGGGCCAGATAAAGGGGCTTTGGTTTTATCT.TT.TCCCCAATAT.CCCGGGGGTGG..GGGGGCCGAGGCCCcgccgc                                                   >  2:1787442/1‑98 (MQ=37)
                                                      tttcGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAA.AACCATCCCTTTCATCT.TG.CCCCAAATATCCCCGGGGGCCCG.GGGGGCAGCGccccccgccc                                                   >  2:5496352/4‑96 (MQ=21)
                                                       gCCGATGCCTCCGGCGGGGATATTTTGGGGAAAATAAAAGGGGTTTGCTTTTTTTT.TTCCCCCAAAAAC.CCCGGGGGGGG..GGGGGCGGAGccccccccgcc                                                  >  2:4715367/1‑95 (MQ=255)
                                                        ccGATGCCTCCGGCGGGGATATTTGGGGGAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGGGC..GGGGGCAGAGCCCCcgccgcccc                                                >  1:2932390/1‑95 (MQ=21)
                                                        ccGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCCcgccgccac                                                >  2:2957496/1‑95 (MQ=255)
                                                           aTGCCTCCGGCGGGGATATTTGGGGCAAGATGAA.ACCCATCCCTTTCATCT.TG.CCCCAAATAT.CCCGGGGGGCCCGGGGGGCAGGGccccccgccacctcc                                              >  2:3815895/1‑91 (MQ=21)
                                                              ccccggggggggTTGTTGGGGACAAGATAAGGGGGTTTTTTTTTTTTC..TT.CCCCAAAT.T.ACCGGGGGGGG..GGGGGGGCAGCCCCCCCGCCGCCACGGTTCa                                        <  2:6556221/93‑1 (MQ=255)
                                                                 cggggggggTTTTTTGGGGAAAGAAAAAGGGG.TTTTTTTTTTTCTCTT.CCCCAAA.AT.CCCCGGGGGGG..GGGGGGGCAGCCCCCCCGCCGCCACGGTTCAcg                                      <  2:1708078/96‑1 (MQ=255)
                                                                 ccGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCAcgc                                     >  2:1361743/1‑101 (MQ=255)
                                                                     cggGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCcgcg                                 >  1:271182/1‑101 (MQ=255)
                                                                      ggggATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                                >  1:5686738/1‑101 (MQ=255)
                                                                        ggATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                              >  2:4834090/1‑101 (MQ=255)
                                                                          atatTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.ACCCAAATAT.CCCGGGGGAGG..GGGGGAAGGGNNCC..NGCCGCCAAGGTTCCCGCAGCGACCgc                            >  1:3977015/1‑100 (MQ=18)
                                                                           tatTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtc                           >  1:2877069/1‑101 (MQ=255)
                                                                            atTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                          >  1:1142901/1‑101 (MQ=255)
                                                                               tGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                       >  1:4182845/1‑101 (MQ=255)
                                                                               tGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                       >  1:799538/1‑101 (MQ=255)
                                                                               tGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                       >  2:4477372/1‑101 (MQ=255)
                                                                               tGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCCTCTCCg                       >  2:1943699/1‑101 (MQ=255)
                                                                                    cAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc                  >  1:6720414/1‑101 (MQ=255)
                                                                                    cAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc                  >  2:279199/1‑101 (MQ=255)
                                                                                    cAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc                  >  1:4291677/1‑101 (MQ=255)
                                                                                    cAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc                  >  1:1895652/1‑101 (MQ=255)
                                                                                     aaGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                 >  1:350862/1‑101 (MQ=255)
                                                                                      aGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGc                >  2:1436274/1‑101 (MQ=255)
                                                                                      aGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGc                >  1:2785758/1‑101 (MQ=255)
                                                                                       gATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc               >  2:1122311/1‑101 (MQ=255)
                                                                                       gATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc               >  2:1595707/1‑101 (MQ=255)
                                                                                        aTAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa              >  2:2738773/1‑101 (MQ=255)
                                                                                        aTAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa              >  2:1473301/1‑101 (MQ=255)
                                                                                         tAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg             >  1:628374/1‑101 (MQ=255)
                                                                                         tAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg             >  2:3723575/1‑101 (MQ=255)
                                                                                          aaaGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc            >  1:250897/1‑101 (MQ=255)
                                                                                           aaGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg           >  2:6022482/1‑101 (MQ=255)
                                                                                            aGGGGCTCTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc          >  2:443185/1‑101 (MQ=255)
                                                                                             ggggCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGcgcg         >  1:1166166/1‑101 (MQ=255)
                                                                                              gggCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcga        >  2:681900/1‑101 (MQ=255)
                                                                                                  tttgctttTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGat    >  2:2439676/1‑101 (MQ=255)
                                                                                                    |                                                                                                           
GGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTATCT.TT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATAT  >  NC_014034/3439698‑3439900

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 29 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG