Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,799 G→C 6.4% intergenic (‑102/+61) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,7990G→C 6.4% 22.1 ‑1.8 ‑0.1219intergenic (‑102/+61)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (5/9):  ref base (114/89):  total (121/98)
Fisher's exact test strand distribution p-value = 1.69e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.83e-01

Only 100 of 312 total aligned reads displayed.

GCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATC  >  NC_014034/3439699‑3439901
                                                                                                     |                                                                                                           
  ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGgctttgct                                                                                                           <  1:6602745/101‑1 (MQ=255)
  ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGgctttgct                                                                                                           <  1:6255691/101‑1 (MQ=255)
   gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGgctttgctt                                                                                                          <  2:1039143/101‑1 (MQ=255)
   gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGgctttgctt                                                                                                          <  2:2128981/101‑1 (MQ=255)
    cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGAT.AAAGGGgctttgcttt                                                                                                         >  2:6556678/1‑101 (MQ=255)
    cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGgctttgcttt                                                                                                         <  2:4312888/101‑1 (MQ=255)
     gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGGAAGAT.AAAGGGGCTTTTCtttt                                                                                                        >  1:886000/1‑101 (MQ=255)
     gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCtttt                                                                                                        <  1:19595/101‑1 (MQ=255)
      aCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTa                                                                                                       <  1:6135412/101‑1 (MQ=255)
       cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGAAGAT.AAAAGGGCTTTGCtttttt                                                                                                      >  2:2841439/1‑99 (MQ=255)
       cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGCAAGATAAAAGGGGGTTTGTttttt                                                                                                       >  1:1618485/1‑100 (MQ=255)
       cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTAt                                                                                                      <  1:873667/101‑1 (MQ=255)
       cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCCCCGGCGGGGGTATTTGGGGGAAGAT.AAAGGGGGTTTGGtttttt                                                                                                      >  2:4405670/1‑99 (MQ=255)
       accTGCACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTAt                                                                                                      <  2:1858758/100‑1 (MQ=255)
         cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTagat                                                                                                    >  1:116295/1‑98 (MQ=255)
           acacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCt.tt                                                                                                 <  2:1168681/101‑1 (MQ=255)
            cacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..c                                                                                              <  2:5439453/101‑1 (MQ=255)
             acGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGGAAGAA.AAAAGGGGTTTTCTTTTTTTt.tttc                                                                                               >  1:2716896/1‑99 (MQ=37)
             acGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGCAAGAT.AAAGGGGCTTTGCTTTTTTTt.tttc                                                                                               >  2:6041504/1‑99 (MQ=255)
             acGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..cc                                                                                             <  2:3069714/101‑1 (MQ=255)
              cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..ccc                                                                                            <  1:316649/101‑1 (MQ=255)
               ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTGGGGCAAGAT.AAAGGGGCGTTGCTTTTTTTT.TT..tccc                                                                                           >  2:4556328/1‑101 (MQ=38)
               ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..cccc                                                                                           <  2:2773400/101‑1 (MQ=255)
                gcgcGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCa                                                                                          <  2:446423/101‑1 (MQ=255)
                  gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCaaa                                                                                        <  1:5407918/101‑1 (MQ=255)
                  gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCaaa                                                                                        <  1:4100347/101‑1 (MQ=255)
                  gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCaaa                                                                                        <  2:6427916/101‑1 (MQ=255)
                   cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAat                                                                                       <  1:2089794/101‑1 (MQ=255)
                   cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAat                                                                                       <  1:1796883/101‑1 (MQ=255)
                   cgACCAGCCTTCGAAAGACACGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAat                                                                                       <  1:4381797/101‑1 (MQ=255)
                    gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGCAGATAAAAGGGGTTTTGTTTTTTTTT.TT..CCCCaaaa                                                                                       >  1:418460/1‑100 (MQ=255)
                    gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTGGGGGAAGAT.AAAGGGGGGTTGGTTTTATCT.TT..CCCCacaaa                                                                                      >  1:6352635/1‑97 (MQ=255)
                    gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAata                                                                                      <  2:7098901/101‑1 (MQ=255)
                     aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAatat                                                                                     <  1:3729819/101‑1 (MQ=255)
                       cAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGCAAGAT.AAAGGGGGTTTGCTTTTTTTT.TT..TCCCCAATATcc                                                                                   >  1:1892807/1‑101 (MQ=38)
                        aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..TCCCAAATATccc                                                                                  >  2:4193104/1‑101 (MQ=255)
                        aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATccc                                                                                  <  2:6526557/101‑1 (MQ=255)
                           tttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGGAAGAT.AAAGGGGGGTTTCTTTTTTTT.TT..CCCCAAAAATTCCggg                                                                               >  2:5150565/1‑101 (MQ=37)
                           tttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGGTTTGCTTTTATCT.TT..CCCCACATATCCCggg                                                                               >  1:718392/1‑101 (MQ=255)
                            ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCgggg                                                                              <  2:2904432/101‑1 (MQ=255)
                               gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCGAGAT.AAAGGGGCTTTGCTTTTATCT.TT..TCCCCAATATCCCcgggggg                                                                           >  2:6340805/1‑99 (MQ=38)
                                aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTGGGGGAAGAA.AAAAGGGCGTTGTTTTTTTTT.TTTCCCCCAAATCCCCCgggggg                                                                            >  1:4763077/1‑100 (MQ=255)
                                  aGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGAATTTGGGGGAAAAT.AAAGGGGCTTTGGTTTTTTCT.TT..CCCCCCAAATCCCgggggggggg                                                                        >  1:1255659/1‑96 (MQ=255)
                                    aCAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGGAAGAT.AAAGGGGGTTTGCTTTTATTT.TT..TCCCAAATATCCCGGGGGTGC..gggg                                                                    >  2:5616182/1‑101 (MQ=37)
                                      aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTTTCT.TT..CCCCCAATATCCCCGGGGGGC..GGGGGc                                                                  >  1:1124096/1‑101 (MQ=37)
                                        gAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCCAATATCCCGGGGGTGC..GGGGGGag                                                                >  2:7004615/1‑101 (MQ=37)
                                             cGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTTGGGGAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGccc                                                           >  2:76352/1‑101 (MQ=255)
                                             cGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGccc                                                           <  1:259569/101‑1 (MQ=255)
                                              ggCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGcccc                                                          <  1:6850273/101‑1 (MQ=255)
                                              ggCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGcccc                                                          <  2:1296618/101‑1 (MQ=255)
                                               gCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGccccc                                                         <  1:2764485/101‑1 (MQ=255)
                                                gtgactttcGATGCCTCCGGCGGGGGTATTTTGGGCAAGAT.GAA.ACCCATCCCCTTCATCT.TT..CCCCCAAAATCCCGGGGGGCCCGGGGGGCAGCGccccc                                                         >  1:2695008/9‑101 (MQ=25)
                                                ccTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCcg                                                        >  1:3284537/1‑101 (MQ=255)
                                                 tgactttcGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.GAA.ACCCATCCCTTTCATCT.TG..CCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGcccccc                                                        <  1:2135005/94‑2 (MQ=25)
                                                 cTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCcgc                                                       >  2:4124060/1‑101 (MQ=255)
                                                   actttcGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.GAA.ACCCATCCCTTTCATCT.TG..CCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGccccccgc                                                      <  1:6051350/96‑4 (MQ=25)
                                                    cGGCCGATGCCTCCGGCGGGGATATTTGGGGCCAGAT.AAAGGGGCTTTGGTTTTATCT.TT..TCCCCAATATCCCGGGGGTGG..GGGGGCCGAGGCCCcgccgc                                                    >  2:1787442/1‑98 (MQ=37)
                                                     tttcGATGCCTCAGGCGGGGATATTTGGGGCAAGAT.GAA.ACCCATCCCTTTCATCT.TG..CCCCAAATATCCCGGGGGGCCCGGGGGGCAGCGccccccccca                                                    >  2:2718379/4‑96 (MQ=21)
                                                     ggCCGATGCCTCCGGCGGGGATATTTTGGGGAAGAT.AAAGGGGCTTTGCTTTTTTCT.TT..CCCCCAATATCCCGGGGGGGC..GGGGGCAGAGccccccccgcc                                                   >  1:5891818/1‑95 (MQ=21)
                                                     ggCCGATGCCTCCGGCGGGGATATTTGGGGCAAGTT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGGGC..GGGGGCAGAGCCCCcgccgcc                                                   >  1:4130178/1‑97 (MQ=255)
                                                      gCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGGGC..GGGGGCAGAGCCCCcgccgcca                                                  >  1:3075811/1‑96 (MQ=255)
                                                       ccGATGCCTCCGGCGGGGATATTTGGGGGAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGGGC..GGGGGCAGAGCCCCcgccgcccc                                                 >  1:2932390/1‑95 (MQ=21)
                                                          aTGCCTCCGGCGGGGATATTTGGGGCAAGAT.GAA.ACCCATCCCTTTCATCT.TG..CCCCAAATATCCCGGGGGGCCCGGGGGGCAGGGccccccgccacctcc                                               >  2:3815895/1‑91 (MQ=21)
                                                          aTGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACggg                                            >  1:387912/1‑100 (MQ=255)
                                                            gCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTc                                          >  2:5760463/1‑101 (MQ=255)
                                                                cggggggggTTTTTTGGGGAAAGAA.AAAGGGG.TTTTTTTTTTTCTCTT..CCCCAAA.ATCCCCGGGGGGG..GGGGGGGCAGCCCCCCCGCCGCCACGGTTCAcg                                       <  2:1708078/96‑1 (MQ=255)
                                                                  ggcggGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGGGC..GGGGGCAGAGCCCC..CGCCGCCACGGGTCAccccg                                    >  1:5523315/1‑101 (MQ=18)
                                                                       ggATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                               >  2:4834090/1‑101 (MQ=255)
                                                                          tatTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtc                            >  1:2877069/1‑101 (MQ=255)
                                                                          tatTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtc                            >  2:4689798/1‑101 (MQ=255)
                                                                           atTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                           >  2:5963222/1‑101 (MQ=255)
                                                                           atTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                           >  2:1452312/1‑101 (MQ=255)
                                                                             ttGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTcc                         >  1:5772179/1‑101 (MQ=255)
                                                                              tGGGGCAAGAT.ACAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                        >  1:6318477/1‑101 (MQ=255)
                                                                              tGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCCTCTCCg                        >  2:1943699/1‑101 (MQ=255)
                                                                                   cAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc                   >  1:3040399/1‑101 (MQ=255)
                                                                                   cAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc                   >  1:6720414/1‑101 (MQ=255)
                                                                                    aaGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                  >  1:4579647/1‑101 (MQ=255)
                                                                                    aaGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                  >  1:350862/1‑101 (MQ=255)
                                                                                      gAT.AAGGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc                >  2:3515738/1‑101 (MQ=255)
                                                                                      gAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc                >  1:1366587/1‑101 (MQ=255)
                                                                                      gAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc                >  2:1122311/1‑101 (MQ=255)
                                                                                      gAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc                >  2:1595707/1‑101 (MQ=255)
                                                                                       aT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa               >  1:1536312/1‑101 (MQ=255)
                                                                                       aT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGCCGccg               >  1:5200695/1‑100 (MQ=25)
                                                                                        t.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg              >  2:3723575/1‑101 (MQ=255)
                                                                                          aagggggTTTTTTTTTTTTCT.CTC.CCCCAAATTCCCCGGGGGGGG..GGGGGGCG.GCCCCCCCCCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa               <  1:4455073/98‑1 (MQ=255)
                                                                                          aaaGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc             >  2:3420106/1‑101 (MQ=255)
                                                                                           aaGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg            >  1:4933775/1‑101 (MQ=255)
                                                                                           aaGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg            >  2:1428373/1‑101 (MQ=255)
                                                                                            aGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc           >  1:4331871/1‑101 (MQ=255)
                                                                                             ggggCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGcgcg          >  2:2664079/1‑101 (MQ=255)
                                                                                              gggCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcga         >  2:681900/1‑101 (MQ=255)
                                                                                                 ctttgctttTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgacga      >  2:5372696/1‑101 (MQ=255)
                                                                                                  tttgctttTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGat     >  2:2439676/1‑101 (MQ=255)
                                                                                                   ttgctttTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGata    >  1:2329643/1‑101 (MQ=255)
                                                                                                   ttgctttTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGata    >  1:3197614/1‑101 (MQ=255)
                                                                                                     gctttTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATc  >  2:2584954/1‑101 (MQ=255)
                                                                                                     gctttTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATc  >  1:6395153/1‑101 (MQ=255)
                                                                                                     gctttTATCA.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATc  >  1:1040084/1‑101 (MQ=255)
                                                                                                     |                                                                                                           
GCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCT.TT..CCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATC  >  NC_014034/3439699‑3439901

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG