Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,804 T→C 5.8% intergenic (‑107/+56) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,8040T→C 5.8% 21.7 ‑2.4 0.0233intergenic (‑107/+56)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (5/9):  ref base (134/85):  total (139/94)
Fisher's exact test strand distribution p-value = 8.93e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 315 total aligned reads displayed.

GACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCA  >  NC_014034/3439704‑3439906
                                                                                                     |                                                                                                          
gACCCTACACGGCGCGGCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCtttt                                                                                                            <  2:1895917/101‑1 (MQ=255)
gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTGGGGGGAGAG.AAAAGGGGTTTGCtttt                                                                                                            >  2:4502665/1‑101 (MQ=255)
  cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGAAGAT.AAAAGGGCTTTGCtttttt                                                                                                          >  2:2841439/1‑99 (MQ=255)
  cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGCAAGATAAAAGGGGGTTTGTttttt                                                                                                           >  1:1618485/1‑100 (MQ=255)
  accTGCACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTAt                                                                                                          <  2:1858758/100‑1 (MQ=255)
   ccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGAT.AAAAGGGGTTTGGttttttt                                                                                                         >  1:3056068/1‑98 (MQ=255)
   ccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATc                                                                                                         <  1:2991409/101‑1 (MQ=255)
    cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTGGGGCAAGAG.AAAGGGGGTTTGCTTTTAttt                                                                                                        >  1:1909414/1‑99 (MQ=39)
    cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTagat                                                                                                        >  1:116295/1‑98 (MQ=255)
     tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGCAGAT.AAAAGGGCTTTGCTTTTTTCtt                                                                                                       >  1:4440987/1‑101 (MQ=255)
        ccGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.cc                                                                                                   <  2:5435566/100‑1 (MQ=255)
        acGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.cc                                                                                                   <  2:3069714/101‑1 (MQ=255)
         cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.ccc                                                                                                  <  1:316649/101‑1 (MQ=255)
          ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.cccc                                                                                                 <  2:3505286/101‑1 (MQ=255)
          ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.cccc                                                                                                 <  2:2773400/101‑1 (MQ=255)
          ggCGCAACCAGCTTGCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.cccc                                                                                                 <  2:2609785/101‑1 (MQ=255)
           gcgcGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGAATTTTGGGGAAGAT.AAAAGGGGTTTGCTTTTTTTTTT.ccccc                                                                                                >  1:2001622/1‑100 (MQ=255)
             gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCaaa                                                                                              <  1:4100347/101‑1 (MQ=255)
             gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCaaa                                                                                              <  1:372520/101‑1 (MQ=255)
             gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCaaa                                                                                              <  1:5407918/101‑1 (MQ=255)
              cgACCAGCTTTCGAAAGACATGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAat                                                                                             <  1:4359064/101‑1 (MQ=255)
               gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTGGGGGAAGAT.AAAGGGGGGTTGGTTTTATCTTT.CCCCacaaa                                                                                            >  1:6352635/1‑97 (MQ=255)
               gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAata                                                                                            <  2:7098901/101‑1 (MQ=255)
                aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAatat                                                                                           <  2:1019151/101‑1 (MQ=255)
                  cAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGCAAGAT.AAAGGGGCGTTGCTTTTTTTTTT.TCCCAAATAT.cc                                                                                        >  1:2877760/1‑101 (MQ=38)
                  cAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.cc                                                                                        <  2:4618377/101‑1 (MQ=255)
                   aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.TCCCAAATAT.ccc                                                                                       >  2:4193104/1‑101 (MQ=255)
                   aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.ccc                                                                                       <  2:6526557/101‑1 (MQ=255)
                       ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGAT.AAAGGGGGTTTGCTTTTTTTTTT.TCCCCAAAAT.CCCgggg                                                                                   >  1:6121268/1‑101 (MQ=255)
                       ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCgggg                                                                                   <  2:2904432/101‑1 (MQ=255)
                          gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGCAAGAT.AAAGGGGGTTTGCTTTTATTTTT.TCCCCAATAT.CCCggggggg                                                                                >  1:6885547/1‑99 (MQ=38)
                          gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCGAGAT.AAAGGGGCTTTGCTTTTATCTTT.TCCCCAATAT.CCCcgggggg                                                                                >  2:6340805/1‑99 (MQ=38)
                           aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGc                                                                               <  2:4368925/101‑1 (MQ=255)
                           aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCAGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTACTTTTATCTTT.CCCCAAATAT.CCCcggggggg                                                                               >  2:5247767/1‑98 (MQ=37)
                            aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..g                                                                            <  1:2841541/101‑1 (MQ=255)
                             aGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGAATTTGGGGGAAAAT.AAAGGGGCTTTGGTTTTTTCTTT.CCCCCCAAAT.CCCgggggggggg                                                                             >  1:1255659/1‑96 (MQ=255)
                                 aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTTTCTTT.CCCCCAATAT.CCCCGGGGGGC..GGGGGc                                                                       >  1:1124096/1‑101 (MQ=37)
                                 aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGc                                                                       <  1:6233208/101‑1 (MQ=255)
                                  ggAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGCAAGAT.AAAGGGGGTTTGCTTTTATCTTT.CCCCCAATAT.CCCGGGGGGGG..ggggggg                                                                      >  1:679386/1‑99 (MQ=37)
                                   gAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCCAATAT.CCCGGGGGTGC..GGGGGGag                                                                     >  2:7004615/1‑101 (MQ=37)
                                        cGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTTGGGGAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGccc                                                                >  2:76352/1‑101 (MQ=255)
                                        cGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGccc                                                                <  1:259569/101‑1 (MQ=255)
                                          gCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGccccc                                                              <  1:4556567/101‑1 (MQ=255)
                                          gCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGccccc                                                              <  1:882130/101‑1 (MQ=255)
                                           ccTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCCcg                                                             <  2:1258793/101‑1 (MQ=255)
                                            tgactttcGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.GAA.ACCCATCCCTTTCATCTTG.CCCCAAATAT.CCCGGGGGGCCCGGGGGGCAGCGcccccc                                                             <  1:2135005/94‑2 (MQ=25)
                                            tgactttcGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.GAA.ACCCATCCCTTTCATCTTG.CCCCAAATAT.CCCGGGGGGCCCGGGGGGCAGCGcccccc                                                             <  2:5759785/94‑2 (MQ=25)
                                            cTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCCcgc                                                            >  2:4124060/1‑101 (MQ=255)
                                               cGGCCGATGCCTCCGGCGGGGATATTTTGGGGAAGAT.AAAGGGGCTTTGCTTTTATCTTT.TCCCAAATAT.CCCCGGGGTGC..GGGGGCCGAGCCCCcgccgc                                                         >  2:1678654/1‑98 (MQ=37)
                                               cGGCCGATGCCTCCGGCGGGGATATTTGGGGCCAGAT.AAAGGGGCTTTGGTTTTATCTTT.TCCCCAATAT.CCCGGGGGTGG..GGGGGCCGAGGCCCcgccgc                                                         >  2:1787442/1‑98 (MQ=37)
                                                tttcGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.GAA.AACCATCCCTTTCATCTTG.CCCCAAATATCCCCGGGGGCCCG.GGGGGCAGCGccccccgccc                                                         >  2:5496352/4‑96 (MQ=21)
                                                tttcGATGCCTCAGGCGGGGATATTTGGGGCAAGAT.GAA.ACCCATCCCTTTCATCTTG.CCCCAAATAT.CCCGGGGGGCCCGGGGGGCAGCGccccccccca                                                         >  2:2718379/4‑96 (MQ=21)
                                                ggCCGATGCCTCCGGCGGGGATATTTTGGGGAAGAT.AAAGGGGCTTTGCTTTTTTCTTT.CCCCCAATAT.CCCGGGGGGGC..GGGGGCAGAGccccccccgcc                                                        >  1:5891818/1‑95 (MQ=21)
                                                ggCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGTGCAGAGCCCCcgccgcc                                                        <  2:5988698/101‑5 (MQ=255)
                                                 gCCGATGCCTCCGGCGGGGATATTTTGGGGAAAAT.AAAAGGGGTTTGCTTTTTTTTTTCCCCCAAAAAC.CCCGGGGGGGG..GGGGGCGGAGccccccccgcc                                                        >  2:4715367/1‑95 (MQ=255)
                                                     aTGCCTCCGGCGGGGATATTTGGGGCAAGAT.GAA.ACCCATCCCTTTCATCTTG.CCCCAAATAT.CCCGGGGGGCCCGGGGGGCAGGGccccccgccacctcc                                                    >  2:3815895/1‑91 (MQ=21)
                                                     aTGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACggg                                                 >  1:387912/1‑100 (MQ=255)
                                                           ccGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGGTCAcgc                                           >  2:5837980/1‑101 (MQ=255)
                                                             ggcggGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGGGC..GGGGGCAGAGCCCC..CGCCGCCACGGGTCAccccg                                         >  1:5523315/1‑101 (MQ=18)
                                                               cggGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCcgcg                                       >  1:271182/1‑101 (MQ=255)
                                                                ggggATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                                      >  1:2743845/1‑101 (MQ=255)
                                                                 gggATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAc                                     >  1:4134508/1‑101 (MQ=255)
                                                                  ggATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                                    >  2:4834090/1‑101 (MQ=255)
                                                                      atTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                                >  2:672834/1‑101 (MQ=255)
                                                                        ttGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTcc                              >  1:5772179/1‑101 (MQ=255)
                                                                         tGGGGCAAGAT.ACAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                             >  1:6318477/1‑101 (MQ=255)
                                                                         tGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                             >  1:799538/1‑101 (MQ=255)
                                                                              cAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc                        >  1:6595071/1‑101 (MQ=255)
                                                                                aGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGc                      >  2:1436274/1‑101 (MQ=255)
                                                                                 gAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc                     >  2:1122311/1‑101 (MQ=255)
                                                                                 gAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc                     >  2:1595707/1‑101 (MQ=255)
                                                                                 gAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGcc                     >  1:1366587/1‑101 (MQ=255)
                                                                                  aT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                    >  2:1473301/1‑101 (MQ=255)
                                                                                  aT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                    >  2:2121171/1‑101 (MQ=255)
                                                                                  aT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                    >  1:1536312/1‑101 (MQ=255)
                                                                                  aT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                    >  1:4204189/1‑101 (MQ=255)
                                                                                  aT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGCCGccg                    >  1:5200695/1‑100 (MQ=25)
                                                                                   t.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg                   >  2:3723575/1‑101 (MQ=255)
                                                                                     aaaGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc                  >  2:3420106/1‑101 (MQ=255)
                                                                                     aaaGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc                  >  1:3318609/1‑101 (MQ=255)
                                                                                      aaGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg                 >  1:4933775/1‑101 (MQ=255)
                                                                                       aGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc                >  1:1880328/1‑101 (MQ=255)
                                                                                       aGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc                >  1:4331871/1‑101 (MQ=255)
                                                                                       aGGGGCTCTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc                >  2:443185/1‑101 (MQ=255)
                                                                                        ggggCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGcgcg               >  1:4621381/1‑101 (MQ=255)
                                                                                         gggCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcga              >  2:681900/1‑101 (MQ=255)
                                                                                            ctttgctttTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgacga           >  2:2499272/1‑101 (MQ=255)
                                                                                              ttgctttTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGata         >  1:3197614/1‑101 (MQ=255)
                                                                                              ttgctttTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGata         >  1:2329643/1‑101 (MQ=255)
                                                                                                gctttTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATc       >  1:6395153/1‑101 (MQ=255)
                                                                                                gctttTATCTTT.CCCCAAATAT.CCCGGGGGCGC..GGGGGCCGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGCCGCCAGCGCGACGATATc       >  1:6052219/1‑101 (MQ=37)
                                                                                                gctttTATCATT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATc       >  1:1040084/1‑101 (MQ=255)
                                                                                                 ctttTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATcc      >  2:1363926/1‑101 (MQ=255)
                                                                                                  ttttATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCca     >  1:352518/1‑101 (MQ=255)
                                                                                                  ttttATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCca     >  1:2093509/1‑101 (MQ=255)
                                                                                                   tttATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcat    >  2:4859960/1‑101 (MQ=255)
                                                                                                    ttATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTNTCCGGGGTCGCCAGCGCGACGATATCcatc   >  1:706427/1‑101 (MQ=255)
                                                                                                    ttATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatc   >  2:6141186/1‑101 (MQ=255)
                                                                                                    ttATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatc   >  2:2239371/1‑101 (MQ=255)
                                                                                                     tATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatca  >  2:5084918/1‑101 (MQ=255)
                                                                                                     |                                                                                                          
GACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTT.CCCCAAATAT.CCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCA  >  NC_014034/3439704‑3439906

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 29 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG