Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,461,382 C→A 95.4% intergenic (‑92/+38) RCAP_rcc03260 ← / ← RCAP_rcc03261 NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,461,3820C→A95.4% 13.9 ‑3.8 ‑0.1172intergenic (‑92/+38)RCAP_rcc03260/RCAP_rcc03261NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (70/94):  ref base (7/1):  total (77/95)
Fisher's exact test strand distribution p-value = 2.30e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.73e-01

Only 100 of 230 total aligned reads displayed.

TCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCG  >  NC_014034/3461283‑3461462
                                                                                                     |                                                                                 
tCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGcc                                                                                   <  2:6015553/101‑1 (MQ=255)
 cTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGcca                                                                                  >  2:791956/1‑100 (MQ=255)
 cTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGcca                                                                                  >  2:2910379/1‑100 (MQ=255)
 cTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGcca                                                                                  >  1:2361536/1‑100 (MQ=255)
  tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccac                                                                                 >  1:5528762/1‑99 (MQ=255)
  tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccac                                                                                 >  1:674834/1‑99 (MQ=255)
  tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccac                                                                                 <  2:2629072/101‑3 (MQ=255)
  tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccac                                                                                 <  2:4148683/101‑3 (MQ=255)
  tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccac                                                                                 >  2:5043340/1‑99 (MQ=255)
  tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccac                                                                                 >  2:69823/1‑99 (MQ=255)
   ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAG.GGGGGCGCAGCCgcc                                                                                >  2:5719136/1‑99 (MQ=255)
   ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacc                                                                                >  1:3946346/1‑98 (MQ=255)
   ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacc                                                                                >  1:3860544/1‑98 (MQ=255)
   ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacc                                                                                >  2:1763409/1‑98 (MQ=255)
   ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacc                                                                                <  2:4038575/101‑4 (MQ=255)
   ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacc                                                                                >  2:3589900/1‑98 (MQ=255)
   ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacc                                                                                >  1:3127714/1‑98 (MQ=255)
    tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccaccc                                                                               >  2:6072025/1‑97 (MQ=255)
    tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccaccc                                                                               >  2:4333917/1‑97 (MQ=255)
    tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccaccc                                                                               >  1:1173602/1‑97 (MQ=255)
    tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTCGCGCAGGGCCAAAGGAA.GGGGGCGCAGccaccc                                                                               <  1:5015684/101‑5 (MQ=255)
     cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacccg                                                                              <  2:318453/101‑6 (MQ=255)
      aTCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacccgg                                                                             >  2:5586517/1‑95 (MQ=255)
        cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacccggcc                                                                           >  1:2851609/1‑93 (MQ=255)
         gCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacccggccg                                                                          <  1:791980/101‑10 (MQ=255)
          cAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacccggccgc                                                                         >  1:4359736/1‑91 (MQ=255)
          cAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacccggccgc                                                                         >  1:4033322/1‑91 (MQ=255)
          cAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacccggccgc                                                                         >  1:1565096/1‑91 (MQ=255)
          cAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacccggccgc                                                                         >  2:2941067/1‑91 (MQ=255)
          cAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGCGCAGccacccggccgc                                                                         >  2:5286376/1‑91 (MQ=255)
                           aCCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCg                                                  >  2:1148531/1‑101 (MQ=255)
                            cccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGt                                                 >  2:3447805/1‑101 (MQ=255)
                             ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                                <  2:5360239/101‑1 (MQ=255)
                              cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                               <  1:1006535/101‑1 (MQ=255)
                              cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                               >  1:2950459/1‑101 (MQ=255)
                               gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                              <  1:6072337/101‑1 (MQ=255)
                                cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                             <  2:4933588/101‑1 (MQ=255)
                                 ggCTTAGCAGGCTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG.CCCC.CC.GGCCGCGCCCCCAACCCCGGTCGTTCGAt                                            <  1:4503283/100‑1 (MQ=255)
                                  gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                           >  2:615219/1‑101 (MQ=255)
                                   cTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAATGGGGC.GCAGCC.ACCCGGCCG...CGC.CCCCC.ACCCCGGCCGTTCGATCa                                          >  2:5835327/1‑101 (MQ=255)
                                   cTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCa                                          <  1:2536688/101‑1 (MQ=255)
                                    ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                         <  2:2873463/101‑1 (MQ=255)
                                     tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTTGATCAGt                                        <  2:674081/101‑1 (MQ=255)
                                      aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                       >  2:7131720/1‑101 (MQ=255)
                                      aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                       >  2:3375971/1‑101 (MQ=255)
                                      aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                       >  1:352481/1‑101 (MQ=255)
                                       gCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTc                                      >  2:2062869/1‑101 (MQ=255)
                                       gCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTc                                      >  1:540099/1‑101 (MQ=255)
                                       gCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTc                                      >  1:929777/1‑101 (MQ=255)
                                         aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGGTGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCtt                                    <  2:2071850/101‑1 (MQ=255)
                                         aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                    >  1:1607054/1‑101 (MQ=255)
                                         aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                    <  2:5118608/101‑1 (MQ=255)
                                         aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                    >  2:3856584/1‑101 (MQ=255)
                                          ggATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTg                                   >  1:1439848/1‑101 (MQ=255)
                                          ggATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTg                                   <  1:5843829/101‑1 (MQ=255)
                                          ggATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTg                                   >  2:582465/1‑101 (MQ=255)
                                           gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                  >  2:5862634/1‑101 (MQ=255)
                                           gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                  >  2:2195819/1‑101 (MQ=255)
                                           gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                  <  2:3946171/101‑1 (MQ=255)
                                            aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                 <  2:1732466/101‑1 (MQ=255)
                                             tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                <  2:778454/101‑1 (MQ=255)
                                             tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                >  1:5552397/1‑101 (MQ=255)
                                             tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                <  2:266706/101‑1 (MQ=255)
                                             gatctgggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                <  2:1208345/96‑1 (MQ=255)
                                               cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                              <  1:756845/101‑1 (MQ=255)
                                               cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                              <  1:5879924/101‑1 (MQ=255)
                                                  gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgagatc                           >  1:1208393/1‑97 (MQ=255)
                                                  gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                           <  2:1852444/101‑1 (MQ=255)
                                                   ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                          <  1:6702441/101‑1 (MQ=255)
                                                   ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                          <  1:2113717/101‑1 (MQ=255)
                                                    gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                         <  1:2730339/101‑1 (MQ=255)
                                                     cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                        <  1:6340557/101‑1 (MQ=255)
                                                     cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                        <  2:1898078/101‑1 (MQ=255)
                                                     cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                        >  2:2541523/1‑101 (MQ=255)
                                                     cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                        <  2:4677862/101‑1 (MQ=255)
                                                       cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGt                      >  1:5653880/1‑101 (MQ=255)
                                                        ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                     >  2:6270836/1‑101 (MQ=255)
                                                        ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                     <  1:2125375/101‑1 (MQ=255)
                                                        ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                     <  2:3697413/101‑1 (MQ=255)
                                                        ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                     >  1:1762156/1‑101 (MQ=255)
                                                        ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                     <  1:6622364/101‑1 (MQ=255)
                                                          ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                   >  1:1657789/1‑101 (MQ=255)
                                                           cAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                  >  1:3553844/1‑101 (MQ=255)
                                                            aGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                 >  1:263801/1‑101 (MQ=255)
                                                             gACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGCTGACGTCAGAgg                >  2:1484072/1‑101 (MQ=255)
                                                              aCGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAGCCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGc               <  1:3601981/101‑1 (MQ=255)
                                                               cGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGcc              <  1:1523597/101‑1 (MQ=255)
                                                                gCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt             >  2:6556905/1‑101 (MQ=255)
                                                                  aGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg           >  2:4648476/1‑101 (MQ=255)
                                                                  aGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg           <  1:5265569/101‑1 (MQ=255)
                                                                    tttGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat         <  1:2013702/101‑1 (MQ=255)
                                                                    tttGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat         >  1:5341571/1‑101 (MQ=255)
                                                                      tGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga       <  1:1913196/101‑1 (MQ=255)
                                                                       ggCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat      <  2:4219351/101‑1 (MQ=255)
                                                                        gcgcAGGGCCAAAGGAA.GGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATc     >  2:3422676/1‑101 (MQ=255)
                                                                         cgcAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg    <  2:3612870/101‑1 (MQ=255)
                                                                          gcAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCCGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgc   >  1:4719619/1‑101 (MQ=255)
                                                                           cAGGGCCAAAGGAA.GGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgcg  >  2:3879410/1‑101 (MQ=255)
                                                                           cAGGGCCAAAGGAA.GGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgcg  >  2:5526474/1‑101 (MQ=255)
                                                                           cAGGGCCAAAGGAA.GGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgcg  >  1:3486827/1‑101 (MQ=255)
                                                                                                     |                                                                                 
TCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCG  >  NC_014034/3461283‑3461462

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG