Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,461,388 C→G 4.1% intergenic (‑98/+32) RCAP_rcc03260 ← / ← RCAP_rcc03261 NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,461,3880C→G 4.1% 8.7 ‑3.1 ‑1.6172intergenic (‑98/+32)RCAP_rcc03260/RCAP_rcc03261NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (6/1):  ref base (70/94):  total (76/96)
Fisher's exact test strand distribution p-value = 4.54e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.34e-02

Only 100 of 218 total aligned reads displayed.

ACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGC  >  NC_014034/3461310‑3461486
                                                                                |                                                                                                   
aCCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCg                                                                          >  2:1148531/1‑101 (MQ=255)
 cccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGt                                                                         >  2:3447805/1‑101 (MQ=255)
  ccGCAAGTTAGCAGGATGCACGGGCGCCGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                                                        <  2:5060634/101‑1 (MQ=255)
   cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                                                       <  1:1006535/101‑1 (MQ=255)
    gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                                                      <  1:6072337/101‑1 (MQ=255)
    gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                                                      >  2:4954240/1‑101 (MQ=255)
    gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                                                      <  1:3390608/101‑1 (MQ=255)
     cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                                                     >  2:4379693/1‑101 (MQ=255)
      ggCTTAGCAGGCTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG.CCCC.CC.GGCCGCGCCCCCAACCCCGGTCGTTCGAt                                                                    <  1:4503283/100‑1 (MQ=255)
       gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                                                   >  2:615219/1‑101 (MQ=255)
       gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                                                   <  2:1623832/101‑1 (MQ=255)
        cTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAATGGGGC.GCAGCC.ACCCGGCCG...CGC.CCCCC.ACCCCGGCCGTTCGATCa                                                                  >  2:5835327/1‑101 (MQ=255)
        cTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCa                                                                  <  1:2536688/101‑1 (MQ=255)
         ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                                                 <  1:5365133/101‑1 (MQ=255)
         ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                                                 <  2:2873463/101‑1 (MQ=255)
         ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                                                 >  1:1195299/1‑101 (MQ=255)
           aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                                               >  2:7131720/1‑101 (MQ=255)
            gCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTc                                                              >  1:929777/1‑101 (MQ=255)
             cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                                             <  1:3120489/101‑1 (MQ=255)
              aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                                            >  2:3856584/1‑101 (MQ=255)
              aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                                            <  1:3516486/101‑1 (MQ=255)
               ggATGCACGGGCGCGGCCAGACGCAGTTTGGCGTAGGGCCAAAGGAA..GGGG.GC.GCAG...CTACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTg                                                           <  2:3399755/101‑1 (MQ=255)
               ggATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTg                                                           >  2:6182803/1‑101 (MQ=255)
               ggATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTg                                                           <  1:5843829/101‑1 (MQ=255)
                gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                                          <  2:3946171/101‑1 (MQ=255)
                gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                                          >  2:4072786/1‑101 (MQ=255)
                gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                                          <  2:4654047/101‑1 (MQ=255)
                gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                                          >  2:5862634/1‑101 (MQ=255)
                 aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                                         <  1:6632138/101‑1 (MQ=255)
                  tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                                        <  2:266706/101‑1 (MQ=255)
                  tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                                        <  2:2071835/101‑1 (MQ=255)
                  tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                                        <  2:1903020/101‑1 (MQ=255)
                  tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                                        <  2:778454/101‑1 (MQ=255)
                    cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                                                      <  1:756845/101‑1 (MQ=255)
                    cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                                                      <  1:5879924/101‑1 (MQ=255)
                    cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                                                      >  1:1459555/1‑101 (MQ=255)
                    cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                                                      <  1:5455777/101‑1 (MQ=255)
                     aCGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacg                                                     >  2:3364711/1‑101 (MQ=255)
                       gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                                                   <  2:1852444/101‑1 (MQ=255)
                        ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                                                  <  1:6702441/101‑1 (MQ=255)
                        ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                                                  <  1:1957617/101‑1 (MQ=255)
                         gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                                                 <  1:2730339/101‑1 (MQ=255)
                          cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                                                >  2:2541523/1‑101 (MQ=255)
                          cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                                                <  2:674814/101‑1 (MQ=255)
                          cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                                                <  1:6340557/101‑1 (MQ=255)
                          cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                                                <  2:4306520/101‑1 (MQ=255)
                           gcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACg                                               <  2:5309180/101‑1 (MQ=255)
                            cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacggcgttct                                              <  2:3772661/101‑8 (MQ=255)
                            cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGt                                              >  1:5653880/1‑101 (MQ=255)
                             ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                                             <  1:2125375/101‑1 (MQ=255)
                             ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                                             <  1:2682456/101‑1 (MQ=255)
                              gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                                            >  1:261360/1‑101 (MQ=255)
                               ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                                           >  1:1657789/1‑101 (MQ=255)
                                cAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                                          <  1:2416446/101‑1 (MQ=255)
                                cAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                                          <  2:5682493/101‑1 (MQ=255)
                                cAGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                                          >  1:3553844/1‑101 (MQ=255)
                                 aGACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                                         >  1:4031206/1‑101 (MQ=255)
                                  gACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGCTGACGTCAGAgg                                        >  2:1484072/1‑101 (MQ=255)
                                  gACGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAgg                                        <  1:2848877/101‑1 (MQ=255)
                                   aCGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAGCCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGc                                       <  1:3601981/101‑1 (MQ=255)
                                   aCGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGc                                       <  2:6481990/101‑1 (MQ=255)
                                    cGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGcc                                      <  2:4732795/101‑1 (MQ=255)
                                    cGCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGcc                                      <  1:1523597/101‑1 (MQ=255)
                                     gCAGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt                                     >  1:2931461/1‑101 (MQ=255)
                                       aGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg                                   <  1:5532663/101‑1 (MQ=255)
                                       aGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg                                   <  1:5265569/101‑1 (MQ=255)
                                       aGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg                                   >  2:662840/1‑101 (MQ=255)
                                       aGTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg                                   <  1:1609073/101‑1 (MQ=255)
                                        gTTTGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga                                  >  1:4319811/1‑101 (MQ=255)
                                          ttGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatg                                >  2:6017651/1‑101 (MQ=255)
                                          ttGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatg                                >  2:5407191/1‑101 (MQ=255)
                                          ttGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatg                                <  2:1352180/101‑1 (MQ=255)
                                           tGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga                               >  2:2299258/1‑101 (MQ=255)
                                           tGGCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga                               <  1:1913196/101‑1 (MQ=255)
                                            ggCGCAGGGCCAAAGGAA.GGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat                              >  2:5890783/1‑101 (MQ=255)
                                            ggCGCAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat                              <  1:2058574/101‑1 (MQ=255)
                                             gcgcAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATc                             <  2:4642029/101‑1 (MQ=255)
                                              cgcAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg                            <  2:3612870/101‑1 (MQ=255)
                                               gcAGGGCCAAAGGAA..GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCCGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgc                           >  1:4719619/1‑101 (MQ=255)
                                                cAGGGCCAAAGGAA.GGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgcg                          >  1:3486827/1‑101 (MQ=255)
                                                cAGGGCCAAAGGAA.GGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgcg                          >  2:3879410/1‑101 (MQ=255)
                                                                     gggggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGcc            >  1:1540280/11‑101 (MQ=255)
                                                                     gggggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGcc            >  2:3588999/11‑101 (MQ=255)
                                                                     gggggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGcc            >  1:811985/11‑101 (MQ=255)
                                                                      ggggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCg           <  1:5558266/92‑1 (MQ=255)
                                                                      ggggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCg           >  1:1273400/10‑101 (MQ=255)
                                                                       gggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGa          <  1:5031604/93‑1 (MQ=255)
                                                                          cgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCt       <  2:1273352/96‑1 (MQ=255)
                                                                          cgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCt       >  1:3931492/6‑101 (MQ=255)
                                                                           gcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCtt      >  2:4239969/5‑101 (MQ=255)
                                                                           gcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCtt      >  2:2117646/5‑101 (MQ=255)
                                                                            cagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTGGCCACCCTTc     >  2:3303748/4‑101 (MQ=255)
                                                                            cagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTc     <  2:1195251/98‑1 (MQ=255)
                                                                            cagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTc     >  2:3546453/4‑101 (MQ=255)
                                                                            cagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTc     >  1:4811929/4‑101 (MQ=255)
                                                                            cagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTc     >  1:7086115/4‑101 (MQ=255)
                                                                             cgccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCa    <  2:3175621/99‑1 (MQ=255)
                                                                             agccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCa    <  2:2850792/99‑1 (MQ=255)
                                                                             agccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCa    >  1:998982/3‑101 (MQ=255)
                                                                               ccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAgc  >  1:369507/1‑101 (MQ=255)
                                                                                |                                                                                                   
ACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGGG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGC  >  NC_014034/3461310‑3461486

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG