Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,498,612 C→T 100% A142T (GCC→ACC)  dapE ← succinyl‑diaminopimelate desuccinylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,498,6120C→T100.0% 1274.1 356A142T (GCC→ACC) dapEsuccinyl‑diaminopimelate desuccinylase
Reads supporting (aligned to +/- strand):  new base (176/172):  ref base (2/0):  total (184/172)

Only 100 of 374 total aligned reads displayed.

TTCATCATGTCTCCCAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGCGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGGGTGGTGGTGACGAAATCGACGGCGGCGGCGACA  >  NC_014034/3498512‑3498709
                                                                                                    |                                                                                                 
ttCATCATGTCTCCCAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGgc                                                                                                   >  2:183905/1‑101 (MQ=255)
  catcatGTCTCCCAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGgtgc                                                                                                 >  1:3148229/1‑101 (MQ=255)
  catcatGTCTCCCAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGgtgc                                                                                                 <  1:2608664/101‑1 (MQ=255)
    tcatGTCTCCCAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCa                                                                                               >  1:6111259/1‑101 (MQ=255)
    tcatGTCTCCCAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGGGCCa                                                                                               >  2:6605365/1‑101 (MQ=255)
    tcatGTCTCCCAAGGTCTCGGGACAGGTCGGCTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCa                                                                                               <  2:6309970/101‑1 (MQ=255)
     catGTCTCCCAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCAt                                                                                              <  1:6297356/101‑1 (MQ=255)
     catGTCTCCCAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCAt                                                                                              >  2:5296646/1‑101 (MQ=255)
      atGTCTCCCAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATc                                                                                             >  2:4018547/1‑101 (MQ=255)
      atGTCTCCCAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATc                                                                                             <  1:1565717/101‑1 (MQ=255)
       tGTCTCCCAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATcc                                                                                            <  1:5789949/101‑1 (MQ=255)
       tGTCTCCCAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATcc                                                                                            <  2:6456074/101‑1 (MQ=255)
        gTCTCCCAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCt                                                                                           <  2:4579280/101‑1 (MQ=255)
        gTCTCCCAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCt                                                                                           <  2:6961961/101‑1 (MQ=255)
          ctcCCAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTg                                                                                         <  2:815808/101‑1 (MQ=255)
           tcCCAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCCGCGCCAGGGTGCCATCCTTGc                                                                                        >  2:2484520/1‑101 (MQ=255)
           tcCCAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGc                                                                                        <  2:4400542/101‑1 (MQ=255)
           tcCCAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGc                                                                                        <  1:4995731/101‑1 (MQ=255)
           tcCCAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGc                                                                                        >  2:1139/1‑101 (MQ=255)
            cccAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTTGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGcc                                                                                       <  1:4193044/101‑1 (MQ=255)
            cccAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGcc                                                                                       <  1:4100/101‑1 (MQ=255)
              cAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCg                                                                                     <  1:1907785/101‑1 (MQ=255)
              cAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGGGCCATCCTTGCCCg                                                                                     >  1:902853/1‑101 (MQ=255)
               aaGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCgg                                                                                    <  1:1443730/101‑1 (MQ=255)
               aaGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCgg                                                                                    <  1:2361710/101‑1 (MQ=255)
                aGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCggg                                                                                   <  2:3701557/101‑1 (MQ=255)
                 ggTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGc                                                                                  <  1:1297400/101‑1 (MQ=255)
                  gTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGcc                                                                                 >  2:3931804/1‑101 (MQ=255)
                    ctcGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCtt                                                                               >  1:3618212/1‑101 (MQ=255)
                    ctcGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCtt                                                                               <  2:1030826/101‑1 (MQ=255)
                    ctcGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCtt                                                                               >  1:6825046/1‑101 (MQ=255)
                    ctcGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCtt                                                                               <  1:1752311/101‑1 (MQ=255)
                      cGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTcc                                                                             >  1:5983628/1‑101 (MQ=255)
                      cGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTcc                                                                             <  1:5342714/101‑1 (MQ=255)
                        ggACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCtc                                                                           <  2:6194786/101‑1 (MQ=255)
                        ggACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCtc                                                                           <  1:2180348/101‑1 (MQ=255)
                             ggTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGc                                                                      <  2:3762038/101‑1 (MQ=255)
                             ggTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGc                                                                      <  2:3009280/101‑1 (MQ=255)
                                cGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCgg                                                                   >  1:5665231/1‑101 (MQ=255)
                                 ggTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCggg                                                                  >  1:2774812/1‑100 (MQ=255)
                                 ggTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGt                                                                  <  2:4678160/101‑1 (MQ=255)
                                 ggTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGt                                                                  >  2:1409566/1‑101 (MQ=255)
                                  gTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTg                                                                 <  1:3701924/101‑1 (MQ=255)
                                    tCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGAt                                                               >  1:2088252/1‑101 (MQ=255)
                                     cGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATc                                                              <  1:6411903/101‑1 (MQ=255)
                                     cGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATc                                                              >  2:4771639/1‑101 (MQ=255)
                                     cGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATc                                                              <  2:1925622/101‑1 (MQ=255)
                                     cGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATc                                                              <  2:5439510/101‑1 (MQ=255)
                                       cccACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGt                                                            <  2:2197842/101‑1 (MQ=255)
                                          accaGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAg                                                         >  1:3385202/1‑101 (MQ=255)
                                            cagacagaCCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGga                                                       >  2:4199138/1‑101 (MQ=255)
                                            cagacagaCCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGga                                                       >  2:2357451/1‑101 (MQ=255)
                                             agacagacCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGgat                                                      >  1:102008/1‑101 (MQ=255)
                                             agacagacCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGgag                                                      >  2:2268131/1‑100 (MQ=255)
                                              gacagacCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGgatg                                                     <  1:1371287/101‑1 (MQ=255)
                                              gacagacCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGgatg                                                     >  1:4617956/1‑101 (MQ=255)
                                                cagacCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGgatgat                                                   <  2:3618063/101‑1 (MQ=255)
                                                 agcccGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATc                                                  <  2:6852299/98‑1 (MQ=255)
                                                 agacCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCGGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATc                                                  >  2:5568029/1‑101 (MQ=255)
                                                 agacCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATc                                                  >  2:4478774/1‑101 (MQ=255)
                                                 agacCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATc                                                  <  1:505964/101‑1 (MQ=255)
                                                  gacCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATcg                                                 <  2:5838366/101‑1 (MQ=255)
                                                   acCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATcgc                                                >  1:734350/1‑101 (MQ=255)
                                                   acCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATcgc                                                >  1:6562233/1‑101 (MQ=255)
                                                       aCATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGccc                                            >  2:3232639/1‑101 (MQ=255)
                                                        cATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCt                                           >  1:2495376/1‑101 (MQ=255)
                                                         ctcgcctcgccCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTc                                          <  1:3045846/100‑1 (MQ=255)
                                                         aTCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTc                                          <  1:5513079/101‑1 (MQ=255)
                                                             cctcgccCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCGATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTcgggc                                      >  2:3450334/1‑101 (MQ=255)
                                                             cctcgccCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTcgggc                                      >  1:3834531/1‑101 (MQ=255)
                                                             ccgcttccTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTcgggc                                      >  2:3523763/7‑101 (MQ=255)
                                                                cgccCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTcgggcggg                                   >  1:4304271/1‑101 (MQ=255)
                                                                cgccCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTcgggcggg                                   >  2:498116/1‑101 (MQ=255)
                                                                 gccCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCgggg                                  <  2:1316464/101‑1 (MQ=255)
                                                                  cccTCGGCCTTCACCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGggt                                 <  2:903070/101‑1 (MQ=255)
                                                                    cTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGggggg                               >  1:4913273/1‑101 (MQ=255)
                                                                     tCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGggtggt                              >  2:5112237/1‑101 (MQ=255)
                                                                     tCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGggtggt                              >  1:6786770/1‑101 (MQ=255)
                                                                     tCGGCCTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGggcggt                              >  1:5433578/1‑101 (MQ=255)
                                                                         ccTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGGgtggtggtg                          <  1:4479013/101‑1 (MQ=255)
                                                                          cTTCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGGGTGGTGGTGa                         >  2:4126591/1‑101 (MQ=255)
                                                                            tCATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGGGTGGTGGTGACg                       <  2:7079029/101‑1 (MQ=255)
                                                                             cATCCAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGGGGGGTGGTGACGa                      >  2:3837066/1‑101 (MQ=255)
                                                                                 cAGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGGGTGGTGGTGACGAAATc                  <  2:59929/101‑1 (MQ=255)
                                                                                  aGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGGGTGGTGGTGACGAAATCg                 <  1:1093361/101‑1 (MQ=255)
                                                                                  aGTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGGGTGGTGGTGACGAAATCg                 >  2:1727750/1‑101 (MQ=255)
                                                                                   gTCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGGGTGGTGGTGACGAAATCGa                >  1:4390657/1‑101 (MQ=255)
                                                                                    tCGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGGGTGGTGGTGACGAACTCGAc               <  2:4843675/101‑1 (MQ=255)
                                                                                     cGAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGGGTGGTGGTGACGAAATCGAcg              <  2:568849/101‑1 (MQ=255)
                                                                                      gAGCAGCGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGGGTGGTGGTGACGAAATCGAcgg             <  1:3299207/101‑1 (MQ=255)
                                                                                       agcagcGCCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGGGTGGTGGTGACGAAATCGAcggc            >  2:1597945/1‑101 (MQ=255)
                                                                                           gcgcCAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGGGTGGTGGTGACGAAATCGAcggcggcg        <  2:6110947/101‑1 (MQ=255)
                                                                                             gcCAGGGTGCCATCCTTGCCCGGGCC.TCCTCGTCGCCGGTGATCGTCAGGATGATCCCGCCCCCGGGCGGGGGGGGGGGTGCGAAAACGAagggggggggc     >  1:2287910/1‑99 (MQ=255)
                                                                                              ccAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGGGTGGTGGTGACGAAATCGACGgcggcggcg     <  2:2674322/101‑1 (MQ=255)
                                                                                               cAGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGGGTGGTGGTGACGAAATCGACGGCGGCGGCGa    >  2:6409472/1‑101 (MQ=255)
                                                                                                aGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGGGTGGTGGTGCCGAAATCGACGGCGGCGGCGAc   >  2:2264015/1‑101 (MQ=255)
                                                                                                aGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGGGTGGTGGTGACGAAATCGACGGCGGCGGCGAc   <  1:4149806/101‑1 (MQ=255)
                                                                                                aGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGGGTGGTGGTGACGAAATCGACGGCGGCGGCGAc   <  2:2857014/101‑1 (MQ=255)
                                                                                                aGGGTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGGGTGGTGGTGACGAAATCGACGGCGGCGGCGAc   <  2:4331862/101‑1 (MQ=255)
                                                                                                 gggTGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGGGTGGTGGTGACGAAATCGACGGCGGCGGCGACa  <  2:5038037/101‑1 (MQ=255)
                                                                                                    |                                                                                                 
TTCATCATGTCTCCCAAGGTCTCGGGACAGGTCGGTTCGCCCACCAGACAGACCGACATCGCCTCGCCCTCGGCCTTCATCCAGTCGAGCAGCGCCAGGGCGCCATCCTTGCCCGGGCCTTCCTCGTCGCCGGTGATCGTCAGGATGATCGCGCCCTCGGGCGGGGTGGTGGTGACGAAATCGACGGCGGCGGCGACA  >  NC_014034/3498512‑3498709

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG