Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,531,673 G→C 100% A433A (GCG→GCC aceA → isocitrate lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,531,6730G→C100.0% 1469.2 389A433A (GCG→GCCaceAisocitrate lyase
Reads supporting (aligned to +/- strand):  new base (185/203):  ref base (0/0):  total (186/203)

Only 100 of 396 total aligned reads displayed.

CTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCGATGG  >  NC_014034/3531575‑3531770
                                                                                                  |                                                                                                 
ctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTg                                                                                                 >  1:6257027/1‑101 (MQ=255)
ctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTg                                                                                                 <  1:2632106/101‑1 (MQ=255)
  ggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGc                                                                                               >  1:755669/1‑101 (MQ=255)
     cgggggCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCa                                                                                            <  1:1333014/98‑1 (MQ=255)
      gAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCaa                                                                                           >  2:3811207/1‑101 (MQ=255)
      gAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCaa                                                                                           >  2:5500191/1‑101 (MQ=255)
      gAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCaa                                                                                           >  2:647145/1‑101 (MQ=255)
       aGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAg                                                                                          >  1:6107059/1‑101 (MQ=255)
          gCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGCcc                                                                                       >  2:521039/1‑101 (MQ=255)
           cACGATCCCCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCt                                                                                      <  1:3753006/101‑1 (MQ=255)
           cACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCt                                                                                      >  2:773553/1‑101 (MQ=255)
              gATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc                                                                                   >  2:4787060/1‑101 (MQ=255)
               aTCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCa                                                                                  >  1:1597031/1‑101 (MQ=255)
                tCACGGAAGCCGCCTACCCGAACGGCCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAg                                                                                 <  2:219926/101‑1 (MQ=255)
                tCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAg                                                                                 <  1:5507716/101‑1 (MQ=255)
                tCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAg                                                                                 >  1:3200894/1‑101 (MQ=255)
                tCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAg                                                                                 <  1:2376951/101‑1 (MQ=255)
                 cACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTGCAGa                                                                                >  2:4525134/1‑101 (MQ=255)
                  aCCGAAGACGCCTACCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAc                                                                               <  2:1248286/101‑1 (MQ=255)
                   ccGAAGCCGCCTATCCGACCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAcc                                                                              <  2:2039470/101‑1 (MQ=255)
                   ccGAAGACGCCTATCCGACGGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAcc                                                                              <  2:134480/101‑1 (MQ=255)
                    cGAAGACGCCTATCGGAACGGGCCCGGGCTGATGAAGCCCGGCTATGACGACGCGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCg                                                                             <  2:5858811/101‑1 (MQ=255)
                    cGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCg                                                                             <  1:5420533/101‑1 (MQ=255)
                     gAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCga                                                                            >  2:4560812/1‑101 (MQ=255)
                      aaGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgag                                                                           >  1:2481977/1‑101 (MQ=255)
                       aGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgaga                                                                          <  2:6010898/101‑1 (MQ=255)
                       aGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCCGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgaga                                                                          <  2:3456528/101‑1 (MQ=255)
                        gACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGAt                                                                         <  1:6897466/101‑1 (MQ=255)
                         aCGCCTACCCGACCGGGCCGGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc                                                                        <  2:371554/101‑1 (MQ=255)
                          cGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcg                                                                       <  1:1079460/101‑1 (MQ=255)
                          cGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcg                                                                       >  1:5308565/1‑101 (MQ=255)
                            cctATCCGAACGGGCCCGGGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcgcg                                                                     <  1:4563940/99‑1 (MQ=255)
                            cctATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcgcg                                                                     <  1:2924386/99‑1 (MQ=255)
                              tATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAc                                                                   >  2:1007233/1‑101 (MQ=255)
                              tATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAc                                                                   <  1:5645181/101‑1 (MQ=255)
                               aTCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAcg                                                                  <  2:3905887/101‑1 (MQ=255)
                               aTCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAcg                                                                  >  1:2146128/1‑101 (MQ=255)
                                 ccGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAcgcg                                                                <  1:1893629/101‑1 (MQ=255)
                                 ccGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAcgcg                                                                >  2:556083/1‑101 (MQ=255)
                                  cGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgg                                                               >  1:2594405/1‑101 (MQ=255)
                                  cGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgg                                                               >  1:4128169/1‑101 (MQ=255)
                                   gAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcggg                                                              >  2:5807420/1‑101 (MQ=255)
                                       gggCCGGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGcgggcggg                                                          <  1:5862866/101‑1 (MQ=255)
                                       gggCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGcgggcggg                                                          <  2:6592293/101‑1 (MQ=255)
                                       gggCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGcgggcggg                                                          >  2:1984236/1‑101 (MQ=255)
                                       gggCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGcgggcggg                                                          <  2:5328348/101‑1 (MQ=255)
                                        ggCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCgggg                                                         >  1:5158714/1‑101 (MQ=255)
                                          ccggAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGgt                                                       <  1:6682823/98‑1 (MQ=255)
                                          cccGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGgt                                                       <  1:4019083/101‑1 (MQ=255)
                                            cGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGgtgt                                                     <  2:3026597/101‑1 (MQ=255)
                                             gAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGtt                                                    <  2:5066178/101‑1 (MQ=255)
                                              aGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTc                                                   <  1:4357424/101‑1 (MQ=255)
                                              aGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTc                                                   <  2:6168785/101‑1 (MQ=255)
                                                cTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCca                                                 >  2:5605141/1‑101 (MQ=255)
                                                cTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCca                                                 <  1:3863807/101‑1 (MQ=255)
                                                cTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCca                                                 >  1:3532604/1‑101 (MQ=255)
                                                 tgatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCcac                                                >  1:4192398/1‑101 (MQ=255)
                                                 tgatgaAGCCCGACTATGACGAAACGCCCCTCGGCCGCGCAGCAGATGCCAGGCTAACGGCCTTCCACGCCGAGAACGCGACACGGACggggggggtgaca                                                >  1:5956578/1‑93 (MQ=255)
                                                  gatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCcaca                                               <  2:1752271/101‑1 (MQ=255)
                                                  gatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCcaca                                               >  1:840467/1‑101 (MQ=255)
                                                   atgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACaa                                              <  2:2966042/101‑1 (MQ=255)
                                                    tgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGGTCAAGGCCTTCCAGACCGAGCTCACGACGCGGGCGGGGGTGCTCCACAAc                                             >  2:3293516/1‑101 (MQ=255)
                                                      acgCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATTCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCt                                           <  2:6737370/99‑1 (MQ=255)
                                                         cccGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGAt                                        >  1:4902307/1‑101 (MQ=255)
                                                         cccGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGAt                                        <  1:2228221/101‑1 (MQ=255)
                                                          ccGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATc                                       <  2:102272/101‑1 (MQ=255)
                                                          ccGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATc                                       <  1:4776710/101‑1 (MQ=255)
                                                              cTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCAccc                                   >  1:3393256/1‑101 (MQ=255)
                                                               tATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCt                                  >  2:6519299/1‑101 (MQ=255)
                                                                aTGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGAACACCCtc                                 >  2:4660044/1‑100 (MQ=255)
                                                                 tGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGc                                <  2:1476524/101‑1 (MQ=255)
                                                                  gacgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGcc                               <  2:6302796/101‑1 (MQ=255)
                                                                  gacgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATAACCCTGcc                               >  2:1134658/1‑101 (MQ=255)
                                                                    cgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCa                             <  1:1007268/101‑1 (MQ=255)
                                                                    cgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACCCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCa                             <  1:6323079/101‑1 (MQ=255)
                                                                     gacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCAc                            <  2:2382577/101‑1 (MQ=255)
                                                                      acacGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCCGCCCAcc                           >  1:4657077/1‑101 (MQ=255)
                                                                       cacGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCt                          >  1:4987946/1‑101 (MQ=255)
                                                                       cacGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGAGATCCACACCCTGAACACACCGCCCAGCt                          >  2:5686377/1‑101 (MQ=255)
                                                                        acGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCtt                         <  2:4300498/101‑1 (MQ=255)
                                                                        acGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCtt                         <  1:3084027/101‑1 (MQ=255)
                                                                          gCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTcc                       <  1:2844821/101‑1 (MQ=255)
                                                                           cccCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCa                      <  2:6856231/101‑1 (MQ=255)
                                                                             cctCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATc                    >  1:4524343/3‑101 (MQ=255)
                                                                              ctCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCt                   <  2:41532/100‑1 (MQ=255)
                                                                              ctCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCt                   <  2:3552084/100‑1 (MQ=255)
                                                                              ctCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCt                   >  2:2841850/2‑101 (MQ=255)
                                                                               tCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTg                  <  1:572694/101‑1 (MQ=255)
                                                                                 ggCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGAc                <  1:6401632/101‑1 (MQ=255)
                                                                                 ggCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGAc                >  1:1684607/1‑101 (MQ=255)
                                                                                   ccGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACcg              <  1:1256951/101‑1 (MQ=255)
                                                                                      cgAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGa           >  2:5923734/1‑101 (MQ=255)
                                                                                      cgAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGa           >  2:1833419/1‑101 (MQ=255)
                                                                                       gAAGCCGCTGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGCCCGCGaa          >  2:2594304/1‑101 (MQ=255)
                                                                                       gAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGaa          >  2:913837/1‑101 (MQ=255)
                                                                                        aaGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGaaa         <  2:1402452/101‑1 (MQ=255)
                                                                                           ccGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCg      >  1:3022927/1‑101 (MQ=255)
                                                                                             gATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCgat    >  1:6748668/1‑101 (MQ=255)
                                                                                               tGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCgatgg  <  1:6544043/101‑1 (MQ=255)
                                                                                               tGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCgatgg  >  2:7044672/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCGATGG  >  NC_014034/3531575‑3531770

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG