Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,541,575 G→C 100% S53S (TCG→TCC prpB → methylisocitrate lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,541,5750G→C100.0% 1281.6 341S53S (TCG→TCCprpBmethylisocitrate lyase
Reads supporting (aligned to +/- strand):  new base (170/171):  ref base (0/0):  total (170/171)

Only 100 of 351 total aligned reads displayed.

GCCCTGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCGGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTCGG  >  NC_014034/3541477‑3541672
                                                                                                  |                                                                                                 
gccCTGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCcgg                                                                                                 >  1:6689883/1‑101 (MQ=255)
 cccTGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGgc                                                                                                >  1:7036340/1‑101 (MQ=255)
  ccTGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGgcg                                                                                               >  1:2380200/1‑101 (MQ=255)
   ccggcccggccgGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGgcgc                                                                                              <  2:6960780/99‑1 (MQ=255)
       cccggcccggCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCgcggcg                                                                                          <  2:6831084/101‑1 (MQ=255)
       cccggcccggCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCgcggcg                                                                                          <  1:3280001/101‑1 (MQ=255)
        ccggcccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGa                                                                                         <  1:3813202/101‑1 (MQ=255)
         cggcccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGAt                                                                                        <  2:1095434/101‑1 (MQ=255)
           gcccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGGTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGt                                                                                      >  1:6403012/1‑101 (MQ=255)
            cccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTc                                                                                     <  1:1279374/101‑1 (MQ=255)
             ccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCg                                                                                    <  1:1018749/101‑1 (MQ=255)
             ccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCg                                                                                    >  1:5980332/1‑101 (MQ=255)
              cggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGcgcgccaaggccgg                                                                                   <  2:6997541/101‑11 (MQ=255)
              cggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCgg                                                                                   >  1:3967798/1‑101 (MQ=255)
                gcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGcc                                                                                 <  1:5304511/101‑1 (MQ=255)
                gcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGcc                                                                                 >  1:1975470/1‑101 (MQ=255)
                  aTCCTGCGCCTGCCGGGCGCGCATAACTGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTc                                                                               >  1:1124709/1‑101 (MQ=255)
                  aTCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTc                                                                               <  2:2769046/101‑1 (MQ=255)
                  aTCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTc                                                                               <  2:3161501/101‑1 (MQ=255)
                  aTCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTc                                                                               <  1:2812018/101‑1 (MQ=255)
                  aTCCTGCGCCTGCCGGGCGCCCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTc                                                                               <  1:6845687/101‑1 (MQ=255)
                    ccTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGa                                                                             >  2:2951402/1‑101 (MQ=255)
                     cTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGAt                                                                            <  1:6155664/101‑1 (MQ=255)
                     cTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGAt                                                                            <  1:5745656/101‑1 (MQ=255)
                          ccTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGcc                                                                       >  2:4193829/1‑101 (MQ=255)
                            tGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTg                                                                     >  2:599129/1‑101 (MQ=255)
                            tGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTg                                                                     <  2:6649715/101‑1 (MQ=255)
                            tGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTg                                                                     >  1:6516419/1‑101 (MQ=255)
                              ccGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGcc                                                                   >  1:925287/1‑101 (MQ=255)
                              ccGAGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATATCGGCCTCGATGGGCCTGcc                                                                   >  1:5624482/1‑101 (MQ=255)
                               cGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCg                                                                  <  2:7062306/101‑1 (MQ=255)
                                gggCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCgg                                                                 <  1:3506973/101‑1 (MQ=255)
                                 ggCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGa                                                                >  2:385928/1‑101 (MQ=255)
                                   cgcgcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGGCTCGATGGGCCTGCCgggac                                                              >  2:3284209/1‑98 (MQ=255)
                                    gcgcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCt                                                             <  2:4684902/101‑1 (MQ=255)
                                     cgcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTc                                                            <  2:5183296/101‑1 (MQ=255)
                                       cATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCgg                                                          >  1:6221341/1‑101 (MQ=255)
                                        aTAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGc                                                         <  1:376493/101‑1 (MQ=255)
                                         tAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCa                                                        >  2:6724290/1‑101 (MQ=255)
                                         tAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCCGACCTCGGCa                                                        >  1:1464982/1‑101 (MQ=255)
                                           aCGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATc                                                      <  2:477860/101‑1 (MQ=255)
                                             ggCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCt                                                    >  2:5734602/1‑101 (MQ=255)
                                             ggCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCt                                                    >  1:3154144/1‑101 (MQ=255)
                                              gCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTg                                                   <  1:185695/101‑1 (MQ=255)
                                               cATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCNGGACCTCGGCATCCTGa                                                  >  2:2612176/1‑101 (MQ=255)
                                                aTGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCCGAc                                                 >  1:1698453/1‑101 (MQ=255)
                                                     cgctCTGCAGGCTAAAGCCGCGGGGTTTCAGGTGTTTTATTTGTCCGGCGCGGCGATGTCGGCCCCGATGGTCCTGCCGGACCTCGGCATCCTGACGGTgg                                            <  2:5765712/101‑1 (MQ=255)
                                                       cgcTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGAc                                          <  2:5227347/101‑1 (MQ=255)
                                                       cgcTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGAc                                          <  1:6064203/101‑1 (MQ=255)
                                                        gcTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACg                                         >  1:6729555/1‑101 (MQ=255)
                                                        gcTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACg                                         <  2:4462345/101‑1 (MQ=255)
                                                        gcTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACg                                         >  2:4095621/1‑101 (MQ=255)
                                                        gcTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACg                                         >  2:4029830/1‑101 (MQ=255)
                                                        gcTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACg                                         >  2:6391064/1‑101 (MQ=255)
                                                        gcTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACg                                         >  2:3027310/1‑101 (MQ=255)
                                                         cTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGa                                        >  1:6785903/1‑101 (MQ=255)
                                                          tGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAg                                       <  1:4897056/101‑1 (MQ=255)
                                                            cAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGt                                     >  2:1554894/1‑101 (MQ=255)
                                                            cAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGt                                     >  2:2887682/1‑101 (MQ=255)
                                                             aGGCGAAAGCCGCGGGGTTTCCGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTc                                    <  2:1344825/101‑1 (MQ=255)
                                                             aGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTc                                    <  1:5420557/101‑1 (MQ=255)
                                                             aGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTc                                    <  2:2007485/101‑1 (MQ=255)
                                                              cttccgatctgcgGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCg                                   <  2:1892500/91‑1 (MQ=255)
                                                                cGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGcc                                 >  1:5941025/1‑101 (MQ=255)
                                                                 gAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCt                                >  1:4981035/1‑101 (MQ=255)
                                                                 gAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCt                                >  2:1735284/1‑101 (MQ=255)
                                                                  aaaGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCtt                               >  2:6458315/1‑101 (MQ=255)
                                                                  aaaGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCtt                               >  1:2939578/1‑101 (MQ=255)
                                                                  aaaGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCtt                               <  1:2601022/101‑1 (MQ=255)
                                                                  aaaGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCtt                               <  2:3967623/101‑1 (MQ=255)
                                                                   aaGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCttt                              <  2:2429781/101‑1 (MQ=255)
                                                                     gCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCttttt                            <  2:6425874/101‑1 (MQ=255)
                                                                     gCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCttttt                            <  2:5357743/101‑1 (MQ=255)
                                                                      ccGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTc                           <  2:3628402/101‑1 (MQ=255)
                                                                       cgcgGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCa                          <  2:6569623/101‑1 (MQ=255)
                                                                         cgGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATc                        >  1:825698/1‑101 (MQ=255)
                                                                          ggggTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATcc                       >  2:2313359/1‑101 (MQ=255)
                                                                          ggggTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATcc                       <  2:3831819/101‑1 (MQ=255)
                                                                          ggggTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTAGATGGGGCTGGCGGCCCTCGGCATCCTGACGGTGGACGGGGGGGCGTTTTTCGTcc                       >  1:2293110/1‑101 (MQ=255)
                                                                           gggTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCg                      >  1:5504861/1‑101 (MQ=255)
                                                                           gggTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCg                      <  1:4041227/101‑1 (MQ=255)
                                                                           gggTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCg                      >  1:4361912/1‑101 (MQ=255)
                                                                            ggTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGc                     >  2:5848510/1‑101 (MQ=255)
                                                                              tttCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCa                   >  1:1608678/1‑101 (MQ=255)
                                                                               ttCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAg                  <  1:5239602/101‑1 (MQ=255)
                                                                                 cAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGAt                >  1:3399561/1‑101 (MQ=255)
                                                                                   ggCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCa              <  1:2887791/101‑1 (MQ=255)
                                                                                     cgcTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCAcg            >  1:2184461/1‑101 (MQ=255)
                                                                                      gcTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCAcgc           >  1:7074535/1‑101 (MQ=255)
                                                                                      gcTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCAcgc           >  2:631940/1‑101 (MQ=255)
                                                                                      gcTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCAcgc           <  1:6028130/101‑1 (MQ=255)
                                                                                       cTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCAcgcg          >  2:5531109/1‑101 (MQ=255)
                                                                                         ttATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCggg        <  2:3490868/101‑1 (MQ=255)
                                                                                         ttATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCggg        >  1:2992601/1‑101 (MQ=255)
                                                                                         ttATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCggg        >  2:5004925/1‑101 (MQ=255)
                                                                                          tATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGc       >  2:5395530/1‑101 (MQ=255)
                                                                                          tATCTGTCCGGCACGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTAGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGc       >  1:5094283/1‑101 (MQ=255)
                                                                                             cTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTc    <  2:6127508/101‑1 (MQ=255)
                                                                                              tGTCCGGCGGCGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTCg   <  1:6288424/101‑1 (MQ=255)
                                                                                               gTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTCgg  >  1:4675756/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GCCCTGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCGGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTCGG  >  NC_014034/3541477‑3541672

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG