Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,591,044 A→G 100% intergenic (‑140/‑120) glnB2 ← / → RCAP_rcc03388 nitrogen regulatory protein P‑II/1A family penicillin‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,591,0440A→G100.0% 1510.4 395intergenic (‑140/‑120)glnB2/RCAP_rcc03388nitrogen regulatory protein P‑II/1A family penicillin‑binding protein
Reads supporting (aligned to +/- strand):  new base (172/223):  ref base (0/0):  total (172/223)

Only 100 of 400 total aligned reads displayed.

CACCGTTATGAGCGTATCAAGGCGCGGGCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCACAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGTTTTCGGCGGTGTAAGTTGCGC  >  NC_014034/3590946‑3591139
                                                                                                  |                                                                                               
cACCGTTATGAGCGTATCAAGGCGCGGGCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGcgca                                                                                               >  2:1891359/1‑101 (MQ=255)
 tacGTTATGAGCGGATCAAGGCGCGGGCAAAGCGCTTCAAACAAGGAAAGCTGCTTAAACCCTCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCaa                                                                                              <  2:1023027/99‑1 (MQ=255)
 aCCGTTATGAGCGTAGCAAGGCGCGGGCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCaa                                                                                              <  2:847309/101‑1 (MQ=255)
   cGTTATGAGCGTATCAAGGCGCGGGCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCANCCGAAAAACGCGCAAAt                                                                                            >  2:174786/1‑101 (MQ=255)
   cGTTATGAGCGTATCAAGGCGCGGGCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAAt                                                                                            >  2:2086896/1‑101 (MQ=255)
     ttATGAGCGTATCAAGGCGCGGGCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATtg                                                                                          >  1:891939/1‑101 (MQ=255)
      tATGAGCGTATCAAGGCGCGGGCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATtgt                                                                                         >  2:6556602/1‑101 (MQ=255)
       aTGAGCGTATCAAGGCGCGGGCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATtgtg                                                                                        >  2:56959/1‑101 (MQ=255)
           gCGTATCAAGGCGCGGGCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCa                                                                                    <  2:1988552/101‑1 (MQ=255)
           gCGTATCAAGGCGCGGGCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCa                                                                                    <  1:6532244/101‑1 (MQ=255)
             gTATCAAGGCGCGGGCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCaga                                                                                  >  1:5544849/1‑101 (MQ=255)
               aTCAAGGCGCGGGCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCCAATTGTGCGCAGAGc                                                                                >  2:7121545/1‑101 (MQ=255)
               aTCAAGGCGCGGGCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGc                                                                                >  1:243523/1‑101 (MQ=255)
                 cAAGGCGCGGGCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCt                                                                              <  1:5232455/101‑1 (MQ=255)
                 cAAGGCGCGGGCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCt                                                                              <  2:42628/101‑1 (MQ=255)
                  aaGGCGCGGGCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCtt                                                                             >  1:6001916/1‑101 (MQ=255)
                   aGGCGCGGGCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTta                                                                            >  1:6703971/1‑101 (MQ=255)
                   aGGCGCGGGCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTta                                                                            <  1:3545699/101‑1 (MQ=255)
                    ggCGCGGGCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTtat                                                                           >  1:4892444/1‑101 (MQ=255)
                        cgGGCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATAttt                                                                       <  1:2372307/101‑1 (MQ=255)
                         gggCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTa                                                                      <  1:6231188/101‑1 (MQ=255)
                         gggCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTa                                                                      >  1:2038448/1‑101 (MQ=255)
                          ggCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGTGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTaa                                                                     <  2:3262540/101‑1 (MQ=255)
                           gCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAACCGCGCAAATTGTGCGCAGAGCCTTATATTTAAg                                                                    <  1:3697782/101‑1 (MQ=255)
                            cAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGc                                                                   <  2:5952770/101‑1 (MQ=255)
                             aaaGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCt                                                                  >  1:4026657/1‑101 (MQ=255)
                              cgatctttCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCtt                                                                 <  1:5936759/95‑1 (MQ=255)
                               aGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCttt                                                                <  2:2590468/101‑1 (MQ=255)
                                gcgcTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTc                                                               <  2:282677/101‑1 (MQ=255)
                                 cgcTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCg                                                              <  2:1181192/101‑1 (MQ=255)
                                   cTTCGCACAAGGAAAGCTGCTTAACCCCGGGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGTGCAGAGCCTTATATTTAAGcagcaata                                                            <  2:2649805/101‑8 (MQ=255)
                                   cTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGaa                                                            <  1:1963116/101‑1 (MQ=255)
                                    ttCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGaaa                                                           >  2:5434063/1‑101 (MQ=255)
                                    ttCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGaaa                                                           <  2:2708385/101‑1 (MQ=255)
                                     tCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGaaaa                                                          >  2:649006/1‑101 (MQ=255)
                                     tCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGaaaa                                                          <  2:3866622/101‑1 (MQ=255)
                                     tCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGaaaa                                                          <  1:3293031/101‑1 (MQ=255)
                                      cGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGaaaaa                                                         <  2:6292380/101‑1 (MQ=255)
                                       gCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAAc                                                        <  1:2555431/101‑1 (MQ=255)
                                        cacaAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAAcc                                                       <  1:3818034/101‑1 (MQ=255)
                                        cacaAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAAcc                                                       <  1:675059/101‑1 (MQ=255)
                                         acaAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCg                                                      <  2:4561520/101‑1 (MQ=255)
                                          caAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGa                                                     <  2:6224793/101‑1 (MQ=255)
                                           aaGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGaa                                                    >  1:6610398/1‑101 (MQ=255)
                                            aGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAAt                                                   >  2:224652/1‑101 (MQ=255)
                                            aGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAAt                                                   <  2:6637733/101‑1 (MQ=255)
                                            aGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAAt                                                   >  2:3027400/1‑101 (MQ=255)
                                            aGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAAt                                                   >  2:4186910/1‑101 (MQ=255)
                                             ggAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATc                                                  <  1:4603325/101‑1 (MQ=255)
                                             ggAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATc                                                  <  1:1845377/101‑1 (MQ=255)
                                              gAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCg                                                 >  1:3918468/1‑101 (MQ=255)
                                              gAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCg                                                 <  1:5361795/101‑1 (MQ=255)
                                               aaaGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGt                                                >  1:3964025/1‑101 (MQ=255)
                                               aaaGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGt                                                >  1:2139837/1‑101 (MQ=255)
                                                aaGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTg                                               <  1:3158625/101‑1 (MQ=255)
                                                aaGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTg                                               <  2:1216408/101‑1 (MQ=255)
                                                aaGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTg                                               >  2:5000828/1‑101 (MQ=255)
                                                aaGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTg                                               <  2:4059359/101‑1 (MQ=255)
                                                   ctgctTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGAcg                                            >  1:109396/1‑101 (MQ=255)
                                                    tgctTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGAcgc                                           >  1:1021942/1‑101 (MQ=255)
                                                    tgctTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGAcgc                                           >  1:2623548/1‑101 (MQ=255)
                                                     gctTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGACAAACCGAATCGTGAcgcg                                          >  2:6695936/1‑101 (MQ=255)
                                                     gctTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGAcgcg                                          >  2:4911452/1‑101 (MQ=255)
                                                      ctTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGa                                         <  1:4340424/101‑1 (MQ=255)
                                                       ttAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAg                                        >  2:3963581/1‑101 (MQ=255)
                                                         aaaCCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGc                                      <  1:1159692/101‑1 (MQ=255)
                                                           aCCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGt                                    >  1:1473362/1‑101 (MQ=255)
                                                              cgcgGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGtctc                                 <  2:4357367/101‑1 (MQ=255)
                                                               gcgGCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTcc                                <  1:4182837/101‑1 (MQ=255)
                                                                 tctaGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCcac                              <  2:6101019/98‑1 (MQ=255)
                                                                 ggCAGTCAAAACCCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCcac                              <  2:3496346/101‑1 (MQ=255)
                                                                 ggCAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCcac                              <  2:1588450/101‑1 (MQ=255)
                                                                   cAGTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACAt                            >  1:6454073/1‑101 (MQ=255)
                                                                     gTCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATcc                          <  2:1464157/101‑1 (MQ=255)
                                                                      tCAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATccc                         <  2:5175553/101‑1 (MQ=255)
                                                                       cAAAAACCGATTCCAGCCGAAAAACGCGCANATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCg                        <  1:2876481/101‑1 (MQ=255)
                                                                       cAAAAACCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCg                        >  1:1268285/1‑101 (MQ=255)
                                                                         aaaaCCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGtt                      <  2:6001611/101‑1 (MQ=255)
                                                                         aaaaCCGATTACAGCCGAAAAACGCGCAAATTGTGCTCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCGCCACATCCCGtt                      <  1:6292438/101‑1 (MQ=255)
                                                                          aaaCCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGttt                     <  2:5885962/101‑1 (MQ=255)
                                                                          aaaCCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGttt                     <  1:4743779/101‑1 (MQ=255)
                                                                          aaaCCGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGttt                     <  2:3519893/101‑1 (MQ=255)
                                                                              cGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGTTTTcgg                 >  2:2350687/1‑101 (MQ=255)
                                                                              cGATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGTTTTcgg                 >  2:3708650/1‑101 (MQ=255)
                                                                               gATTCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGTTTTcggc                >  2:6772356/1‑101 (MQ=255)
                                                                                  tCCAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGTTTTCGGCGgt             >  1:4961087/1‑101 (MQ=255)
                                                                                    cAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGTTTTCGGCGgtgt           <  2:6874980/101‑1 (MQ=255)
                                                                                    cAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGTTTTCGGCGgtgt           <  2:5459177/101‑1 (MQ=255)
                                                                                    cAGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGTTTTCGGCGgtgt           >  1:7107381/1‑101 (MQ=255)
                                                                                     aGCCGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGTTTTCGGCGGTGTa          <  2:5788653/101‑1 (MQ=255)
                                                                                       ccGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGTTTTCGGCGGTGTAAg        <  1:342323/101‑1 (MQ=255)
                                                                                       ccGAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGTTTTCGGCGGTGTAAg        <  2:5544559/101‑1 (MQ=255)
                                                                                         gAAAAACGCGCAAATTGTGCGCAGATCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGTTTTCGGCGGTGTAAGtt      >  1:82239/1‑101 (MQ=255)
                                                                                         gAAAAACGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGTTTTCGGCGGTGTAAGtt      >  2:4397157/1‑101 (MQ=255)
                                                                                          aaaaaCGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGTTTTCGGCGGTGTAAGTTg     >  2:4712819/1‑101 (MQ=255)
                                                                                           aaaaCGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCGACATCCCGTTTTCGGCGGTGTAAGTTgc    <  1:170859/101‑1 (MQ=255)
                                                                                           aaaaCGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGTTTTCGGCGGTGTAAGTTgc    <  1:2471153/101‑1 (MQ=255)
                                                                                           aaaaCGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGTTTTCGGCGGTGTAAGTTgc    >  2:2443727/1‑101 (MQ=255)
                                                                                            aaaCGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGTTTTCGGCGGTGTAAGTTgcg   >  1:5810664/1‑101 (MQ=255)
                                                                                             aaCGCGCAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGTTTTCGGCGGTGTAAGTTgcgc  <  2:3358709/101‑1 (MQ=255)
                                                                                                  |                                                                                               
CACCGTTATGAGCGTATCAAGGCGCGGGCAAAGCGCTTCGCACAAGGAAAGCTGCTTAAACCCGCGGCAGTCAAAAACCGATTCCAGCCGAAAAACGCACAAATTGTGCGCAGAGCCTTATATTTAAGCTTTCGAAAAACCGAATCGTGACGCGAGGCGTCTCCACATCCCGTTTTCGGCGGTGTAAGTTGCGC  >  NC_014034/3590946‑3591139

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG