Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,609,116 G→A 1.2% intergenic (+55/‑116) clpB → / → dinB chaperone ClpB/DNA polymerase IV

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,609,1160G→A 1.2% 2.3 ‑1.7 ‑0.0403intergenic (+55/‑116)clpB/dinBchaperone ClpB/DNA polymerase IV
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (180/218):  total (184/219)
Fisher's exact test strand distribution p-value = 1.83e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 422 total aligned reads displayed.

GGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGT.GGGGGCTCTG.CC.CCCGCTTCGCTCCCCCCGGGATATTT.G.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.GGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGC  >  NC_014034/3609019‑3609215
                                                                                                       |                                                                                                    
ggCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAgg                                                                                                      <  2:6581432/101‑1 (MQ=38)
 gccagccaGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGt                                                                                                     >  2:5595688/1‑101 (MQ=38)
 gccagccaGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGt                                                                                                     <  1:6684025/101‑1 (MQ=38)
  ccagccagCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTa                                                                                                    <  1:4687952/101‑1 (MQ=38)
    agccagcGCGTCCAGACCGAACCGCGCGTGCTGAACTGCTACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTAG.G.GCAAGGTaaa                                                                                                  <  1:5093489/101‑1 (MQ=37)
     gccagcGCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTaaaa                                                                                                 >  2:6231264/1‑101 (MQ=38)
     gccagcGCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTaaaa                                                                                                 <  2:6714332/101‑1 (MQ=38)
     gccagcGCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTaaaa                                                                                                 <  1:807040/101‑1 (MQ=38)
             gTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGcc                                                                                         <  2:5506765/101‑1 (MQ=38)
              tCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGccc                                                                                        >  2:5970030/1‑101 (MQ=37)
              tCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGccc                                                                                        <  2:2189342/101‑1 (MQ=38)
              tCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTCccc                                                                                        <  1:5597096/101‑1 (MQ=37)
                cAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCtt                                                                                      <  2:1976320/101‑1 (MQ=38)
                cAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCtt                                                                                      >  1:3438069/1‑101 (MQ=37)
                 tgACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCttt                                                                                     <  1:3876800/100‑1 (MQ=38)
                 aGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCttt                                                                                     <  2:212721/101‑1 (MQ=38)
                 aGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCttt                                                                                     <  1:4338362/101‑1 (MQ=38)
                 aGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCttt                                                                                     <  1:4134385/101‑1 (MQ=38)
                      gAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCtt                                                                                >  2:6912648/1‑101 (MQ=37)
                      gAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCtt                                                                                <  2:2733003/101‑1 (MQ=38)
                        aCCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGc                                                                              <  1:1382435/101‑1 (MQ=38)
                           gcgcgTGCCGAACTGATGCGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTT.G.GAGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTc                                                                           <  2:2914273/101‑1 (MQ=37)
                            cgcgTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCa                                                                          <  2:5898947/101‑1 (MQ=38)
                             gcgTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAg                                                                         <  1:4034858/101‑1 (MQ=38)
                              cgTGCTGAACTGATACGGGAGTGGGGGGCTTTGCCC.CCCGCTTCGCTCCCCCCCGGATATTTGG.G.GCAAGGGAAAAGCGTTGCCCTTTATCTTGCCTCTGa                                                                        >  1:3376358/1‑101 (MQ=255)
                               gTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGat                                                                       >  2:5418272/1‑101 (MQ=37)
                               gTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGat                                                                       >  2:1356175/1‑101 (MQ=37)
                                tGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGata                                                                      >  1:2527993/1‑101 (MQ=37)
                                tGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGata                                                                      <  1:4960106/101‑1 (MQ=38)
                                tGCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGata                                                                      <  1:4462566/101‑1 (MQ=38)
                                 gCTGAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGatat                                                                     >  1:7017510/1‑101 (MQ=37)
                                    gAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATccc                                                                  <  1:5944782/101‑1 (MQ=37)
                                    gAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATccc                                                                  >  2:6163919/1‑101 (MQ=37)
                                    gAACTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATccc                                                                  >  1:5338381/1‑101 (MQ=37)
                                     aaCTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCg                                                                 >  1:1883687/1‑101 (MQ=37)
                                     aaCTGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCg                                                                 <  1:1467418/101‑1 (MQ=37)
                                        tGATACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCgggg                                                              >  1:3190135/1‑101 (MQ=37)
                                          aTACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGt                                                            <  2:2821528/101‑1 (MQ=38)
                                           tACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTg                                                           <  1:3756773/101‑1 (MQ=37)
                                           tACGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTg                                                           >  2:3349670/1‑101 (MQ=37)
                                            aCGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGa                                                          <  1:5536689/101‑1 (MQ=37)
                                            aCGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGa                                                          >  1:6909269/1‑101 (MQ=25)
                                            aCGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCCGGATATTTGG.G.GCAAGGTAAACGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGa                                                          >  1:299732/1‑101 (MQ=37)
                                             cGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGaa                                                         >  2:1058802/1‑101 (MQ=25)
                                             cGGGAGTGGGGGGCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGaa                                                         <  2:4762946/101‑1 (MQ=37)
                                              gggAGTGGGGGGCTCTG.CCGCCGGCTTCGCGCCCCCCGGGGGATTTGG.G.GCAAGGTAAAAGCATTGCGCTTTAGCTTGCCTCAGAGATCCCgggggcgtgt                                                        >  2:2254919/1‑96 (MQ=255)
                                                 agtggggggCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAAtttt                                                     <  2:6392426/97‑1 (MQ=37)
                                                  gtggggggCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTg                                                    <  2:6813769/98‑1 (MQ=37)
                                                     gggggCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGgcg                                                 <  1:3138438/101‑1 (MQ=37)
                                                      ggggCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGgcgc                                                >  2:6506560/1‑101 (MQ=37)
                                                      ggggCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGgcgc                                                >  1:5884698/1‑101 (MQ=37)
                                                       gggCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCa                                               >  2:4105621/1‑101 (MQ=37)
                                                         gCTCTGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGc                                             <  1:2885509/101‑1 (MQ=37)
                                                          ctctGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGcc                                            >  2:2652525/1‑101 (MQ=37)
                                                          ctctGCCC.CCCGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGcc                                            <  2:2056284/101‑1 (MQ=37)
                                                           tctgccc.cccGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCa                                           <  1:715498/96‑1 (MQ=255)
                                                            ctgccc.cccGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCaa                                          >  1:348309/5‑101 (MQ=255)
                                                            ctgccc.cccGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCaa                                          >  1:2001603/5‑101 (MQ=255)
                                                              gccc.cccGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCaaaa                                        >  1:5443994/3‑101 (MQ=255)
                                                                 c.cccGCTTCGCTCCCCCCGGGATATTT.GAG.GCGAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga.g                                    >  1:6272088/1‑101 (MQ=255)
                                                                     cGCTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gggg                                 >  2:3989611/1‑101 (MQ=255)
                                                                       cTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gggggc                               <  2:3437945/101‑3 (MQ=255)
                                                                       cTTCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gggggc                               >  2:5373264/1‑99 (MQ=255)
                                                                        ttCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gggggca                              <  2:5021704/101‑4 (MQ=255)
                                                                        ttCGCTCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gggggca                              <  2:3700948/101‑4 (MQ=255)
                                                                             tCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcc                          >  1:3099844/1‑101 (MQ=21)
                                                                             tCCCCCCGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcc                          >  2:2987206/1‑101 (MQ=21)
                                                                              ccccgcGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                         <  1:2255014/101‑1 (MQ=21)
                                                                              ccccccGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                         <  1:6506890/101‑1 (MQ=21)
                                                                              ccccccGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                         >  1:6128991/1‑101 (MQ=21)
                                                                                ccccGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                       <  1:126944/101‑1 (MQ=21)
                                                                                ccccGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                       >  2:4921046/1‑101 (MQ=21)
                                                                                 cccGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                      <  2:1454125/101‑1 (MQ=21)
                                                                                   cGGGATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTcc                    >  1:6372303/1‑101 (MQ=21)
                                                                                    gggATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTccc                   >  2:6996182/1‑101 (MQ=21)
                                                                                    gggATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTccc                   >  2:2681984/1‑101 (MQ=21)
                                                                                    gggATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTccc                   >  1:5681065/1‑101 (MQ=21)
                                                                                      gATATTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTccccc                 >  2:4208961/1‑101 (MQ=21)
                                                                                       atatTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCa                >  1:1054010/1‑101 (MQ=21)
                                                                                       atatTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCa                <  2:157509/101‑1 (MQ=18)
                                                                                       atatTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCa                <  1:2363994/101‑1 (MQ=18)
                                                                                        tatTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCAc               <  2:4591097/101‑1 (MQ=18)
                                                                                        tatTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCAc               <  2:437404/101‑1 (MQ=18)
                                                                                        tatTTGG.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCAc               <  2:3132588/101‑1 (MQ=18)
                                                                                         atttgg.g.gCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACg              >  2:2459182/6‑101 (MQ=255)
                                                                                         atttgg.g.gCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACg              >  1:1127044/6‑101 (MQ=255)
                                                                                         atttgg.g.gCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACg              <  1:1669874/96‑1 (MQ=255)
                                                                                          tttgg.g.gCAAGGTAACAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACgg             <  1:3278816/97‑1 (MQ=18)
                                                                                            tgg.g.gCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGcc           >  2:3857056/3‑101 (MQ=255)
                                                                                            tgg.g.gCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGcc           >  1:2708988/3‑101 (MQ=255)
                                                                                             gg.g.gCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCg          >  2:6348085/2‑101 (MQ=255)
                                                                                              g.g.gCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCgg         <  2:1221088/101‑1 (MQ=255)
                                                                                                   cAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTc      >  2:880871/1‑101 (MQ=255)
                                                                                                   cAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTc      >  1:3563534/1‑101 (MQ=255)
                                                                                                   cAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTc      >  1:4202569/1‑101 (MQ=255)
                                                                                                   cAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTc      <  2:1782931/101‑1 (MQ=255)
                                                                                                   cAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTc      <  2:517692/101‑1 (MQ=255)
                                                                                                     aGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCt    >  1:1693383/1‑101 (MQ=255)
                                                                                                       gTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGc  <  1:1787832/101‑1 (MQ=255)
                                                                                                       gTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGc  >  2:5737793/1‑101 (MQ=255)
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GGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGT.GGGGGCTCTG.CC.CCCGCTTCGCTCCCCCCGGGATATTT.G.G.GCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.GGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGC  >  NC_014034/3609019‑3609215

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG