Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,609,127 G→T 1.0% intergenic (+66/‑105) clpB → / → dinB chaperone ClpB/DNA polymerase IV

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,609,1270G→T 1.0% 2.4 ‑1.0 ‑0.0426intergenic (+66/‑105)clpB/dinBchaperone ClpB/DNA polymerase IV
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (214/206):  total (218/208)
Fisher's exact test strand distribution p-value = 3.73e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.89e-01

Only 100 of 442 total aligned reads displayed.

GCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGT.GGGGGCTCTG.CCCCCGCTTCGCTCCCCCCGGGATATTT.GGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.GGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGA  >  NC_014034/3609030‑3609226
                                                                                                    |                                                                                                    
gcgTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTg                                                                                                      >  1:4023152/1‑101 (MQ=37)
   tCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGccc                                                                                                   >  1:5650200/1‑101 (MQ=37)
   tCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGccc                                                                                                   <  2:2189342/101‑1 (MQ=38)
   tCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGccc                                                                                                   >  2:5970030/1‑101 (MQ=37)
     cAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCtt                                                                                                 >  1:3453518/1‑101 (MQ=37)
     cAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCtt                                                                                                 <  1:3692659/101‑1 (MQ=38)
      tgACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCttt                                                                                                <  1:3876800/100‑1 (MQ=38)
      aGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCttt                                                                                                <  2:122037/101‑1 (MQ=38)
      aGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCttt                                                                                                <  2:573471/101‑1 (MQ=38)
       gACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTa                                                                                               <  1:4134025/101‑1 (MQ=38)
       gACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTa                                                                                               >  2:6580168/1‑101 (MQ=37)
       gACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTa                                                                                               <  2:5133349/101‑1 (MQ=38)
           gAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATGGCCCTTTATCtt                                                                                           <  1:5124517/101‑1 (MQ=37)
            aaCCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTg                                                                                          <  2:1921391/101‑1 (MQ=38)
            aaCCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTg                                                                                          <  1:1236688/101‑1 (MQ=38)
            aaCCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGAATTGCCCTTTATCTTg                                                                                          >  2:2800067/1‑101 (MQ=37)
              ccGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGcc                                                                                        <  2:3603023/101‑1 (MQ=38)
                    gTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGat                                                                                  <  2:6312089/101‑1 (MQ=38)
                    gTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGat                                                                                  <  2:3907768/101‑1 (MQ=38)
                     tGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGata                                                                                 <  1:4462566/101‑1 (MQ=38)
                     tGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGata                                                                                 <  1:2928869/101‑1 (MQ=38)
                     tGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGata                                                                                 <  2:5335168/101‑1 (MQ=38)
                        tGAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGAATTGCCCTTTTTCTGTCCTCCGATATcc                                                                              >  2:6948141/1‑101 (MQ=25)
                         gAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATccc                                                                             >  2:6163919/1‑101 (MQ=37)
                         gAACTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATccc                                                                             <  1:5944782/101‑1 (MQ=37)
                          aaCTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCg                                                                            <  2:63870/101‑1 (MQ=37)
                           aCTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCgg                                                                           <  2:4276784/101‑1 (MQ=37)
                            cTGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCggg                                                                          <  2:3376235/101‑1 (MQ=37)
                             tGATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCgggg                                                                         >  1:21757/1‑101 (MQ=37)
                              gATACGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCggggg                                                                        >  2:1692590/1‑101 (MQ=37)
                                 aCGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGa                                                                     >  1:6909269/1‑101 (MQ=25)
                                  cGGGAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGaa                                                                    <  2:4762946/101‑1 (MQ=37)
                                   gggAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAAt                                                                   <  2:2372058/101‑1 (MQ=37)
                                    ggAGTGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAAtt                                                                  >  2:2825769/1‑101 (MQ=25)
                                       gtggggggCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTg                                                               >  1:4764543/4‑101 (MQ=37)
                                        tggggggCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTgg                                                              >  1:3164575/3‑101 (MQ=37)
                                          gggggCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCNGGGGGTGAATTTTGgcg                                                            >  2:2726294/1‑101 (MQ=37)
                                           gggtctgtgccccccGCTTCGCTCCCCCCGTGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGTATTTTGgcgc                                                           <  2:681704/91‑1 (MQ=37)
                                            gggCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCa                                                          >  2:4105621/1‑101 (MQ=37)
                                             ggCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAg                                                         <  1:6715915/101‑1 (MQ=37)
                                               ctctGCCCCCCGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGcc                                                       <  2:2056284/101‑1 (MQ=37)
                                                tctgccccccGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                      >  1:4956646/6‑101 (MQ=255)
                                                tctgccccccGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                      >  1:609605/6‑101 (MQ=255)
                                                 ctgccccccGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCaa                                                     >  1:5575702/5‑101 (MQ=255)
                                                 ctgccccccGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCaa                                                     >  1:326681/5‑101 (MQ=255)
                                                 ctgccccccGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCaa                                                     <  1:1417652/97‑1 (MQ=255)
                                                   gccccccGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCaaaa                                                   <  2:3343235/99‑1 (MQ=255)
                                                    ccccccGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag                                                  >  1:4197969/2‑101 (MQ=255)
                                                       cccGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gg                                              <  1:1880583/101‑1 (MQ=255)
                                                         cGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gggg                                            >  2:3989611/1‑101 (MQ=255)
                                                         cGCTTCGCTCCCCCCGGGATATTTGGGGCAAGGCAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGAGAATTTTGCCGCAGCCAAAAGC.gggg                                            >  2:2653384/1‑101 (MQ=37)
                                                          gCTTCGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.ggggg                                           >  1:3419234/1‑100 (MQ=255)
                                                              cGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gggggcagc                                       <  2:2577181/101‑6 (MQ=255)
                                                              cGCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gggggcagc                                       >  1:1969760/1‑96 (MQ=255)
                                                               gCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                                       >  2:2986459/1‑101 (MQ=25)
                                                               gCTCCCCCCGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                                       <  1:2330827/101‑1 (MQ=25)
                                                                  ccccccGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                    >  1:6245099/1‑101 (MQ=21)
                                                                  ccccccGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                    >  2:6720381/1‑101 (MQ=21)
                                                                    ccccGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                                  >  2:4921046/1‑101 (MQ=21)
                                                                    ccccGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                                  <  2:5414077/101‑1 (MQ=21)
                                                                    ccccGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                                  >  1:2439062/1‑101 (MQ=21)
                                                                     cccGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                 <  2:1454125/101‑1 (MQ=21)
                                                                      ccGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTc                                <  1:1675362/101‑1 (MQ=21)
                                                                       cGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTcc                               >  1:3560701/1‑101 (MQ=21)
                                                                        gggATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTccc                              >  1:1520467/1‑101 (MQ=21)
                                                                        gggATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTccc                              <  1:7082524/101‑1 (MQ=21)
                                                                          gATATTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTccccc                            >  1:2539407/1‑101 (MQ=21)
                                                                           atatTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCa                           >  1:1054010/1‑101 (MQ=21)
                                                                           atatTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCa                           >  1:3086442/1‑101 (MQ=21)
                                                                           atatTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCa                           <  2:5495741/101‑1 (MQ=18)
                                                                            tatgtggggCAGGGTAAAGGCATTTCACTTTGTCTTGCCTCGGATATCCCGGGGGTGAATTTTGGCGGAGCCAAAAGAGGGGGCAGCGCCCCCTCACGCAc                          <  2:224896/95‑1 (MQ=9)
                                                                            tatTTGGGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCAc                          <  2:3132588/101‑1 (MQ=18)
                                                                             atttggggCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTTAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACg                         <  1:725672/96‑1 (MQ=21)
                                                                             atttggggCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACg                         >  1:3125631/6‑101 (MQ=255)
                                                                             atttggggCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACg                         >  1:1127044/6‑101 (MQ=255)
                                                                              tttggggCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACgg                        <  1:544611/97‑1 (MQ=255)
                                                                               ttggggCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCTCCCACGGc                       >  2:3751944/4‑101 (MQ=18)
                                                                                tggggCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGcc                      >  1:6505339/3‑101 (MQ=255)
                                                                                  gggCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCgg                    <  2:4498958/101‑1 (MQ=255)
                                                                                    gCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCgggc                  >  1:2780733/1‑100 (MQ=255)
                                                                                    gCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGt                  <  1:5373536/101‑1 (MQ=255)
                                                                                     cAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTc                 >  2:880871/1‑101 (MQ=255)
                                                                                     cAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTc                 <  2:1782931/101‑1 (MQ=255)
                                                                                     cAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTc                 >  1:4303931/1‑101 (MQ=255)
                                                                                       aGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCt               >  1:1693383/1‑101 (MQ=255)
                                                                                       aGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCt               <  1:913788/101‑1 (MQ=255)
                                                                                         gTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGc             <  1:1787832/101‑1 (MQ=255)
                                                                                           aaaaGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGt           >  1:5853595/1‑101 (MQ=255)
                                                                                           aaaaGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGt           >  2:4620605/1‑101 (MQ=255)
                                                                                            aaaGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGtt          >  2:1365348/1‑101 (MQ=255)
                                                                                              aGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAg        >  2:5831939/1‑101 (MQ=255)
                                                                                               gCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGa       <  2:5641084/101‑1 (MQ=255)
                                                                                               gCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGa       >  1:2672149/1‑101 (MQ=255)
                                                                                               gCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGa       >  2:2095027/1‑101 (MQ=255)
                                                                                                cATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGGCCTGCGTTAGaa      <  1:6352093/101‑1 (MQ=255)
                                                                                                 aTTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAAt     <  2:6496053/101‑1 (MQ=255)
                                                                                                  ttGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGTCCGGGTCCTGCGTTAGAAtt    >  2:1060618/1‑101 (MQ=255)
                                                                                                   tGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGTCCGGGTCCTGCGGTAGAATTg   >  1:1271265/1‑101 (MQ=18)
                                                                                                   tGCCCTTTATCTTGCCTCAGATAGCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTg   <  2:7033611/101‑1 (MQ=255)
                                                                                                    gCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATGGa  >  1:5716939/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
GCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGT.GGGGGCTCTG.CCCCCGCTTCGCTCCCCCCGGGATATTT.GGGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.GGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGA  >  NC_014034/3609030‑3609226

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG