Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,609,130 C→T 1.2% intergenic (+69/‑102) clpB → / → dinB chaperone ClpB/DNA polymerase IV

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,609,1300C→T 1.2% 3.9 ‑1.0 ‑0.0420intergenic (+69/‑102)clpB/dinBchaperone ClpB/DNA polymerase IV
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (213/202):  total (217/203)
Fisher's exact test strand distribution p-value = 3.73e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.16e-01

Only 100 of 444 total aligned reads displayed.

TCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGT.GGGGGCTCTG.CCCCC..GCTTCGCTCCCCCCGGGATATTT.G.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.GGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCA  >  NC_014034/3609033‑3609229
                                                                                                       |                                                                                                    
tCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGccc                                                                                                      >  2:5970030/1‑101 (MQ=37)
   aGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCttt                                                                                                   <  1:4134385/101‑1 (MQ=38)
   aGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCttt                                                                                                   <  2:459906/101‑1 (MQ=38)
   aGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCttt                                                                                                   <  2:573471/101‑1 (MQ=38)
    gACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTa                                                                                                  <  1:3731959/101‑1 (MQ=38)
    gACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTa                                                                                                  >  2:6580168/1‑101 (MQ=37)
    gACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTa                                                                                                  >  1:5933999/1‑101 (MQ=37)
       cGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCt                                                                                               <  1:6720885/101‑1 (MQ=38)
        gAACCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCtt                                                                                              >  2:5536399/1‑101 (MQ=37)
         aaCCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTg                                                                                             <  2:1921391/101‑1 (MQ=38)
         aaCCGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTg                                                                                             <  1:1236688/101‑1 (MQ=38)
           ccGCGCGTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGcc                                                                                           <  2:2954925/101‑1 (MQ=38)
            cgcgcgTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCt                                                                                          <  2:4076622/101‑1 (MQ=38)
               gcgTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAg                                                                                       <  1:5960285/101‑1 (MQ=38)
                 gTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGat                                                                                     <  1:6916960/101‑1 (MQ=38)
                 gTGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGat                                                                                     <  2:6312089/101‑1 (MQ=38)
                  tGCTGAACTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGata                                                                                    >  1:5206920/1‑101 (MQ=37)
                     tGAACTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGAATTGCCCTTTTTCTGTCCTCCGATATcc                                                                                 >  2:6948141/1‑101 (MQ=25)
                      gAACTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATccc                                                                                >  2:6163919/1‑101 (MQ=37)
                      gAACTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATccc                                                                                <  2:3979357/101‑1 (MQ=37)
                      gAACTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATccc                                                                                <  2:4203396/101‑1 (MQ=37)
                       aaCTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCg                                                                               <  2:63870/101‑1 (MQ=37)
                       aaCTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCg                                                                               >  1:1883687/1‑101 (MQ=37)
                       aaCTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCg                                                                               <  2:6490113/101‑1 (MQ=37)
                         cTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCggg                                                                             >  2:2041333/1‑101 (MQ=37)
                           gATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCGTGCCTCAGATATCCCggggg                                                                           >  1:4369180/1‑101 (MQ=37)
                            aTACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGt                                                                          <  2:2714473/101‑1 (MQ=37)
                            aTACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGt                                                                          <  2:2821528/101‑1 (MQ=38)
                               cGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGaa                                                                       <  1:1533653/101‑1 (MQ=37)
                                     tggggggCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTgg                                                                 >  1:947206/3‑101 (MQ=37)
                                     tggggggCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTgg                                                                 >  1:3164575/3‑101 (MQ=37)
                                       gggggCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGgcg                                                               >  1:4826564/1‑101 (MQ=37)
                                        gggtctgtgcccccc..GCTTCGCTCCCCCCGTGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGTATTTTGgcgc                                                              <  2:681704/91‑1 (MQ=37)
                                          ggCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAg                                                            >  2:248599/1‑101 (MQ=37)
                                          ggCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAg                                                            >  2:3736803/1‑101 (MQ=37)
                                           gCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGc                                                           <  1:2885509/101‑1 (MQ=37)
                                           gCTCTG.CCCCCCGGCTTCGCTCCCCCCGGGATATTT.GCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggccaaagg                                                            >  2:3115225/1‑92 (MQ=2)
                                            ctctGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGcc                                                          >  2:2363900/1‑101 (MQ=37)
                                             tctgcccccc..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                         >  1:1794536/6‑101 (MQ=255)
                                             tctgcccccc..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                         <  1:3995438/96‑1 (MQ=25)
                                              ctgcccccc..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCaa                                                        >  1:2001603/5‑101 (MQ=255)
                                                 cccccc..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag                                                     >  1:4197969/2‑101 (MQ=255)
                                                  ccccc..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga                                                    >  2:2237457/1‑101 (MQ=255)
                                                   cccc..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGTTGAATTTTGGCGCAGCCAAAaga.g                                                  >  1:735810/1‑101 (MQ=255)
                                                      c..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gggg                                               >  1:1449694/1‑101 (MQ=255)
                                                      c..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGCAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGAGAATTTTGCCGCAGCCAAAAGC.gggg                                               >  2:2653384/1‑101 (MQ=37)
                                                         gCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.ggggg                                              >  1:3419234/1‑100 (MQ=255)
                                                          cTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gggggc                                             >  2:5373264/1‑99 (MQ=255)
                                                          cTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gggggc                                             <  2:3437945/101‑3 (MQ=255)
                                                          cTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gggggc                                             >  1:3467647/1‑99 (MQ=255)
                                                           ttCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gggggca                                            <  1:2337481/101‑4 (MQ=255)
                                                            tCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gggggcag                                           >  2:1072397/1‑97 (MQ=255)
                                                             cGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gggggcagc                                          <  1:5418544/101‑6 (MQ=255)
                                                                 ccccccGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGTGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                       >  1:4600557/1‑101 (MQ=21)
                                                                 ccccccGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                       <  1:6506890/101‑1 (MQ=21)
                                                                 ccccccGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                       >  2:6720381/1‑101 (MQ=21)
                                                                 ccccccGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                       >  1:3132701/1‑101 (MQ=21)
                                                                 ccccccGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                       >  1:6245099/1‑101 (MQ=21)
                                                                 ccccccGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                       >  1:6128991/1‑101 (MQ=21)
                                                                   ccccGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                                     <  2:208535/101‑1 (MQ=21)
                                                                    cccGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                    <  2:1454125/101‑1 (MQ=21)
                                                                    cccGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                    <  2:21756/101‑1 (MQ=21)
                                                                     ccGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTc                                   <  2:4826321/101‑1 (MQ=21)
                                                                      cGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTcc                                  >  1:6372303/1‑101 (MQ=21)
                                                                      cGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTcc                                  >  1:3560701/1‑101 (MQ=21)
                                                                       gggATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTccc                                 >  1:5681065/1‑101 (MQ=21)
                                                                         gATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTccccc                               <  1:296230/101‑1 (MQ=18)
                                                                          atatTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCa                              >  1:1054010/1‑101 (MQ=21)
                                                                          atatTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCa                              >  1:3086442/1‑101 (MQ=21)
                                                                          atatTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCa                              >  1:5477109/1‑101 (MQ=18)
                                                                           tatTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCAc                             <  2:5455841/101‑1 (MQ=18)
                                                                            atttgg.ggCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACg                            >  1:1127044/6‑101 (MQ=255)
                                                                            atttgg.ggCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACg                            >  1:2187288/6‑101 (MQ=255)
                                                                             tttgg.ggCAAGGTAACAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACgg                           <  1:3278816/97‑1 (MQ=18)
                                                                              ttgg.ggCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGc                          >  1:1766733/4‑101 (MQ=255)
                                                                              ttgg.ggCAAGGTAAAAGCATCGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGc                          <  1:5595978/98‑1 (MQ=18)
                                                                               tgg.ggCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGcc                         >  1:2708988/3‑101 (MQ=255)
                                                                                 g.ggCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCgg                       <  2:1221088/101‑1 (MQ=255)
                                                                                 g.ggCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCgg                       <  2:4498958/101‑1 (MQ=255)
                                                                                     cAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTc                    >  2:880871/1‑101 (MQ=255)
                                                                                     cAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTc                    <  1:1677278/101‑1 (MQ=255)
                                                                                     cAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTc                    <  2:517692/101‑1 (MQ=255)
                                                                                     cAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTc                    >  1:4992827/1‑101 (MQ=255)
                                                                                       aGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCt                  >  1:2468526/1‑101 (MQ=255)
                                                                                           aaaaGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGt              >  1:3877919/1‑101 (MQ=255)
                                                                                            aaaGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGtt             >  2:3863565/1‑101 (MQ=255)
                                                                                            aaaGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGtt             >  1:2589692/1‑101 (MQ=255)
                                                                                            aaaGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGtt             >  2:1365348/1‑101 (MQ=255)
                                                                                            aaaGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGtt             <  2:325871/101‑1 (MQ=255)
                                                                                             aaGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTa            >  1:7117077/1‑101 (MQ=255)
                                                                                              aGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAg           >  2:5831939/1‑101 (MQ=255)
                                                                                                cagtGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGaa         <  2:1746671/98‑1 (MQ=255)
                                                                                                 aTTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAAt        >  1:5786794/1‑101 (MQ=255)
                                                                                                 aTTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAAt        <  2:3352453/101‑1 (MQ=255)
                                                                                                   tGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGTCCGGGTCCTGCGGTAGAATTg      >  1:1271265/1‑101 (MQ=18)
                                                                                                   tGCCCTTTATCTTGCCTCAGATAGCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTg      <  2:7033611/101‑1 (MQ=255)
                                                                                                    gCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGa     >  2:2758044/1‑101 (MQ=255)
                                                                                                    gCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGa     >  2:4761344/1‑101 (MQ=255)
                                                                                                      ccTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATc   <  2:5046025/101‑1 (MQ=255)
                                                                                                       cTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCa  >  2:1673421/1‑101 (MQ=255)
                                                                                                       |                                                                                                    
TCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGT.GGGGGCTCTG.CCCCC..GCTTCGCTCCCCCCGGGATATTT.G.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.GGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCA  >  NC_014034/3609033‑3609229

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG