Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,609,153 G→A 1.2% intergenic (+92/‑79) clpB → / → dinB chaperone ClpB/DNA polymerase IV

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,609,1530G→A 1.2% 2.4 ‑1.0 ‑0.0410intergenic (+92/‑79)clpB/dinBchaperone ClpB/DNA polymerase IV
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (221/184):  total (225/185)
Fisher's exact test strand distribution p-value = 3.84e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.97e-01

Only 100 of 441 total aligned reads displayed.

AACTGATACGGGAGT.GGGGGCTCTG.CCCCC..GCTTCGCTCCCCCCGGGATATTT.G.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.GGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCACCATGCCCGCGCTTTGCCGTGAT  >  NC_014034/3609056‑3609252
                                                                                                       |                                                                                                    
aaCTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCg                                                                                                      <  2:6490113/101‑1 (MQ=37)
aaCTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCg                                                                                                      >  2:3417805/1‑101 (MQ=37)
 aCTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCgg                                                                                                     <  2:4276784/101‑1 (MQ=37)
 aCTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCgg                                                                                                     <  2:1865653/101‑1 (MQ=37)
  cTGATACGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCggg                                                                                                    >  1:6729935/1‑101 (MQ=37)
       aCGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGa                                                                                               >  1:6909269/1‑101 (MQ=25)
       aCGGGAGTGGGGGGCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGa                                                                                               <  1:5536689/101‑1 (MQ=37)
           gagtggggggCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAAttt                                                                                           <  2:6575510/96‑1 (MQ=37)
           gagtggggggCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAAttt                                                                                           <  1:4122835/96‑1 (MQ=37)
            agtggggggCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAAtttt                                                                                          <  1:3431142/97‑1 (MQ=37)
             gtggggggCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTg                                                                                         >  1:6441932/4‑101 (MQ=37)
              tggggggCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTgg                                                                                        >  1:3164575/3‑101 (MQ=37)
                 ggggCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGgcgc                                                                                     >  1:5884698/1‑101 (MQ=37)
                  gggCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCa                                                                                    >  2:4105621/1‑101 (MQ=37)
                   ggCTCTGCCCCCC..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAg                                                                                   <  2:33441/101‑1 (MQ=38)
                   ggCTCTG.CCCCCCGGCTTCGCTCCCCCCGGGATATTT.GCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggccaaag                                                                                    >  2:6064346/1‑93 (MQ=2)
                      tctgcccccc..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                                                <  2:5169106/96‑1 (MQ=255)
                      tctgcccccc..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                                                >  1:1794536/6‑101 (MQ=255)
                      tctgcccccc..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                                                <  1:3995438/96‑1 (MQ=25)
                       ctgcccccc..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCaa                                                                               <  1:1417652/97‑1 (MQ=255)
                        tgcccccc..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCaaa                                                                              <  2:3142860/98‑1 (MQ=255)
                        tgcccccc..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCaaa                                                                              >  2:5812703/4‑101 (MQ=255)
                         gcccccc..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCaaaa                                                                             <  1:6274761/99‑1 (MQ=255)
                         gcccccc..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCaaaa                                                                             >  1:5443994/3‑101 (MQ=255)
                          cccccc..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag                                                                            >  1:4197969/2‑101 (MQ=255)
                           ccccc..GCTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga                                                                           >  2:2237457/1‑101 (MQ=255)
                                   cTTCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gggggc                                                                    <  2:2008459/101‑3 (MQ=255)
                                    ttCGCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gggggca                                                                   <  2:5021704/101‑4 (MQ=255)
                                       gCTCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                                                                 <  1:482084/101‑1 (MQ=25)
                                         tCCCCCCGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcc                                                               >  2:2987206/1‑101 (MQ=21)
                                          ccccccGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                                              <  1:6506890/101‑1 (MQ=21)
                                          ccccccGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                                              >  1:4672587/1‑101 (MQ=21)
                                          ccccccGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                                              >  1:3132701/1‑101 (MQ=21)
                                            ccccGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                                                            <  1:126944/101‑1 (MQ=21)
                                             cccGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                                           <  2:21756/101‑1 (MQ=21)
                                             cccGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                                           <  2:1454125/101‑1 (MQ=21)
                                             cccGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                                           <  2:6247335/101‑1 (MQ=21)
                                              ccGGGATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTc                                                          <  1:1675362/101‑1 (MQ=21)
                                                gggATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTccc                                                        >  2:4811669/1‑101 (MQ=21)
                                                gggATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTccc                                                        <  1:7082524/101‑1 (MQ=21)
                                                gggATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTccc                                                        >  1:1520467/1‑101 (MQ=21)
                                                gggATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTccc                                                        <  1:3349793/101‑1 (MQ=21)
                                                  gATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTccccc                                                      >  1:2539407/1‑101 (MQ=21)
                                                  gATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTccccc                                                      <  1:296230/101‑1 (MQ=18)
                                                  gATATTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTccccc                                                      <  1:568616/101‑1 (MQ=18)
                                                   atatTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTcccccc                                                     >  2:6950741/1‑100 (MQ=18)
                                                   atatTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCa                                                     >  2:2061872/1‑101 (MQ=21)
                                                   atatTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCa                                                     >  1:965270/1‑101 (MQ=21)
                                                   atatTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCa                                                     >  1:1054010/1‑101 (MQ=21)
                                                   atatTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCa                                                     <  1:2363994/101‑1 (MQ=18)
                                                    tatTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCAc                                                    <  2:5455841/101‑1 (MQ=18)
                                                    tatTTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCAc                                                    >  2:4662338/1‑101 (MQ=21)
                                                     atttgg.ggCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTTAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACg                                                   <  1:725672/96‑1 (MQ=21)
                                                     atttgg.ggCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACg                                                   >  1:2187288/6‑101 (MQ=255)
                                                       ttgg.ggCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCTCCCACGGc                                                 >  2:3751944/4‑101 (MQ=18)
                                                       ttgg.ggCAAGGTAAAAGCATCGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGc                                                 <  1:5595978/98‑1 (MQ=18)
                                                        tgg.ggCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGcc                                                >  2:282453/3‑101 (MQ=255)
                                                             gCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGt                                            <  1:5373536/101‑1 (MQ=255)
                                                              cAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTc                                           >  2:880871/1‑101 (MQ=255)
                                                              cAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTc                                           >  1:4992827/1‑101 (MQ=255)
                                                              cAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTc                                           >  1:3563534/1‑101 (MQ=255)
                                                                aGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCt                                         >  1:1693383/1‑101 (MQ=255)
                                                                aGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCt                                         <  1:3061004/101‑1 (MQ=255)
                                                                    aaaaGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGt                                     >  1:3911022/1‑101 (MQ=255)
                                                                    aaaaGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGt                                     >  2:3573855/1‑101 (MQ=255)
                                                                     aaaGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGtt                                    >  1:1900563/1‑101 (MQ=255)
                                                                     aaaGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGtt                                    >  2:1365348/1‑101 (MQ=255)
                                                                     aaaGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGtt                                    >  2:3970555/1‑101 (MQ=255)
                                                                        gCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGa                                 >  2:5680004/1‑101 (MQ=255)
                                                                        gCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGa                                 >  1:2672149/1‑101 (MQ=255)
                                                                          aTTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAAt                               >  2:6755854/1‑101 (MQ=255)
                                                                          aTTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAAt                               >  1:5786794/1‑101 (MQ=255)
                                                                          aTTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAAt                               >  1:853153/1‑101 (MQ=255)
                                                                          aTTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAAt                               >  1:2328227/1‑101 (MQ=255)
                                                                           ttGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAAtt                              >  2:2643645/1‑101 (MQ=255)
                                                                           ttGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAAtt                              <  2:1107243/101‑1 (MQ=255)
                                                                            tGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTg                             >  1:6175733/1‑101 (MQ=255)
                                                                            tGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTg                             >  1:1203764/1‑101 (MQ=255)
                                                                             gCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGa                            >  1:2139708/1‑101 (MQ=255)
                                                                                cTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCa                         >  2:1673421/1‑101 (MQ=255)
                                                                                cTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCa                         >  2:2744786/1‑101 (MQ=255)
                                                                                 tttATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCAc                        >  1:113358/1‑101 (MQ=255)
                                                                                  ttATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCAcc                       <  1:3736953/101‑1 (MQ=255)
                                                                                   tATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCACCa                      <  2:6385867/101‑1 (MQ=255)
                                                                                   tATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCACCa                      >  1:4578365/1‑101 (MQ=255)
                                                                                   tATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCACCa                      >  1:51860/1‑101 (MQ=255)
                                                                                     tCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCACCATg                    <  1:6045325/101‑1 (MQ=255)
                                                                                     tCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCACCATg                    >  2:750490/1‑101 (MQ=255)
                                                                                      cTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCACCATGc                   >  2:5914216/1‑101 (MQ=255)
                                                                                       ttGCCTCAGATATCCCGGGTGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCACCATGcc                  <  1:2825871/101‑1 (MQ=255)
                                                                                       ttGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCACCATGcc                  >  2:4539171/1‑101 (MQ=255)
                                                                                          ccTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCACCATGCCcgc               >  2:6841402/1‑101 (MQ=255)
                                                                                          ccTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCACCATGCCcgc               >  1:6657809/1‑101 (MQ=255)
                                                                                           cTCAGATATCCCGGGGGTGAATTTTGGCACAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGCATTGATCACCATGCCcgcg              >  1:2750861/1‑101 (MQ=25)
                                                                                              aGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCACCATGCCCGCGCtt           <  2:2527899/101‑1 (MQ=255)
                                                                                               gATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCACCATGCCCGCGCttt          <  1:3523562/101‑1 (MQ=255)
                                                                                               gATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCACCATGCCCGCGCttt          <  2:2398314/101‑1 (MQ=255)
                                                                                               gATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGCCCTGCGTTAGAATTGATCACCATGCCCGCGCttt          >  2:2692942/1‑101 (MQ=255)
                                                                                                 tatCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGGTCACCATGCCCGCGCTTTGc        >  1:4364046/1‑101 (MQ=255)
                                                                                                 tatCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCACCATGCCCGCGCTTTGc        <  2:227264/101‑1 (MQ=255)
                                                                                                       |                                                                                                    
AACTGATACGGGAGT.GGGGGCTCTG.CCCCC..GCTTCGCTCCCCCCGGGATATTT.G.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.GGGGCAGCGCCCCCTCCCCCACGGCCGGGTCCTGCGTTAGAATTGATCACCATGCCCGCGCTTTGCCGTGAT  >  NC_014034/3609056‑3609252

Base quality scores:  ATCG  < 3 ≤  ATCG  < 26 ≤  ATCG  < 32 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG