Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 367,286 G→A 100% A214T (GCC→ACC)  bztD → glutamate/aspartate ABC transporter ATP‑binding protein BztD

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034367,2860G→A100.0% 1462.1 390A214T (GCC→ACC) bztDglutamate/aspartate ABC transporter ATP‑binding protein BztD
Reads supporting (aligned to +/- strand):  new base (202/187):  ref base (0/0):  total (202/188)

Only 100 of 398 total aligned reads displayed.

TCGACGAACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCGCCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCG  >  NC_014034/367188‑367383
                                                                                                  |                                                                                                 
 cgacgaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCAccc                                                                                                >  2:167108/1‑101 (MQ=255)
  gacgaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCa                                                                                               >  1:3518479/1‑101 (MQ=255)
     gaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATga                                                                                            >  2:154446/1‑101 (MQ=255)
     gaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATga                                                                                            >  2:3081244/1‑101 (MQ=255)
     gaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATga                                                                                            >  1:3119127/1‑101 (MQ=255)
       aCCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATgaga                                                                                          <  1:1025563/101‑1 (MQ=255)
         ccACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATg                                                                                        <  2:7041047/101‑1 (MQ=255)
         ccACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATg                                                                                        <  1:4311433/101‑1 (MQ=255)
          cACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATgg                                                                                       <  2:1237710/101‑1 (MQ=255)
          cACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATgg                                                                                       <  2:3659386/101‑1 (MQ=255)
           aCCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATggg                                                                                      >  2:832349/1‑101 (MQ=255)
             cTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCt                                                                                    <  1:3361829/101‑1 (MQ=255)
               cGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCttt                                                                                  <  2:6551036/101‑1 (MQ=255)
               cGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCttt                                                                                  <  1:3972512/101‑1 (MQ=255)
                ggCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTg                                                                                 <  2:1842366/101‑1 (MQ=255)
                 gcgcTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGc                                                                                >  2:5793248/1‑101 (MQ=255)
                  cgcTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGcc                                                                               >  1:1112681/1‑101 (MQ=255)
                   gcTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGccc                                                                              <  2:3210483/101‑1 (MQ=255)
                   gcTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGccc                                                                              <  1:4789917/101‑1 (MQ=255)
                   gcTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGccc                                                                              <  1:3685886/101‑1 (MQ=255)
                    cTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCa                                                                             <  1:1812376/101‑1 (MQ=255)
                       gACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGCCGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGc                                                                          <  2:1951177/101‑1 (MQ=255)
                       gACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGc                                                                          >  2:5861092/1‑101 (MQ=255)
                           ccGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTg                                                                      <  2:6778183/101‑1 (MQ=255)
                           ccGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTg                                                                      >  2:5957499/1‑101 (MQ=255)
                             gagaTGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGc                                                                    >  2:2597961/1‑101 (MQ=255)
                             gagaTGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGc                                                                    <  2:4192851/101‑1 (MQ=255)
                              agaTGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCg                                                                   >  1:679822/1‑101 (MQ=255)
                               gatgatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGa                                                                  >  2:5531815/1‑101 (MQ=255)
                                atgatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGaa                                                                 >  2:6278407/1‑101 (MQ=255)
                                  gcgcAAGGGAGTGCTGGGTGGCATCAACCAGCTCCCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAAcc                                                               <  2:5696815/98‑1 (MQ=21)
                                  gatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGCAcc                                                               >  2:3310961/1‑101 (MQ=255)
                                  gatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAAcc                                                               <  1:2196706/101‑1 (MQ=255)
                                    tCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCgg                                                             >  1:2072153/1‑101 (MQ=255)
                                    tCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCgg                                                             >  2:2851141/1‑101 (MQ=255)
                                    tCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCgg                                                             <  1:2361432/101‑1 (MQ=255)
                                    tCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCgg                                                             >  2:22459/1‑101 (MQ=255)
                                     cAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCggg                                                            <  2:6479888/101‑1 (MQ=255)
                                       aGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTg                                                          >  1:4626865/1‑101 (MQ=255)
                                         gAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGCCGCACCGGGTGAt                                                        >  2:5386870/1‑101 (MQ=255)
                                          aaGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATc                                                       >  1:4866927/1‑101 (MQ=255)
                                          aaGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATc                                                       >  2:1492151/1‑101 (MQ=255)
                                           aGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCt                                                      <  1:5478098/101‑1 (MQ=255)
                                            gTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCtt                                                     >  1:7011543/1‑101 (MQ=255)
                                              gCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCa                                                   <  2:6078778/101‑1 (MQ=255)
                                              gCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCa                                                   >  1:913748/1‑101 (MQ=255)
                                               cTGGATACGATGCTCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCAt                                                  >  1:6406051/1‑101 (MQ=255)
                                               cTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCAt                                                  <  1:3292799/101‑1 (MQ=255)
                                               cTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCAt                                                  <  1:6576132/101‑1 (MQ=255)
                                               cTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCAt                                                  <  2:3925907/101‑1 (MQ=255)
                                                tGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATg                                                 <  2:6930967/101‑1 (MQ=255)
                                                 ggATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATgg                                                >  1:1510377/1‑101 (MQ=255)
                                                  gATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGc                                               <  2:1058683/101‑1 (MQ=255)
                                                   aTACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGcc                                              <  1:791573/101‑1 (MQ=255)
                                                      cGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGAc                                           <  1:5327227/101‑1 (MQ=255)
                                                      cGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGAc                                           <  1:6119119/101‑1 (MQ=255)
                                                       gatgatCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACg                                          >  2:472464/1‑101 (MQ=255)
                                                        atgatCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACgg                                         <  1:4483602/101‑1 (MQ=255)
                                                          gatCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGc                                       <  1:667383/101‑1 (MQ=255)
                                                            tCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTAACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGGAg                                     >  2:4286738/1‑101 (MQ=255)
                                                               aGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtc                                  <  1:265564/101‑1 (MQ=255)
                                                               aGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtc                                  <  1:3428643/101‑1 (MQ=255)
                                                               aGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtc                                  >  1:4986234/1‑101 (MQ=255)
                                                                  tCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcgtc                               <  1:6020668/101‑1 (MQ=255)
                                                                   cGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAGCCGGGTGATCTTCATGGCCGACGGGCAGAtcgtcg                              >  2:3371631/1‑101 (MQ=255)
                                                                   cGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcgtcg                              <  2:929071/101‑1 (MQ=255)
                                                                   cGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcgtcg                              <  2:4011572/101‑1 (MQ=255)
                                                                       gAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCa                          >  2:4609863/1‑101 (MQ=255)
                                                                        aGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAg                         <  1:1280167/101‑1 (MQ=255)
                                                                         ggAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGa                        >  1:3465986/1‑101 (MQ=255)
                                                                           aaGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaac                      >  1:5643684/1‑101 (MQ=255)
                                                                           aaGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAAACGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaac                      >  2:6625395/1‑101 (MQ=255)
                                                                            aGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaaca                     >  2:2054335/1‑101 (MQ=255)
                                                                            aGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaaca                     <  1:6255804/101‑1 (MQ=255)
                                                                            aGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaaca                     >  2:1771393/1‑101 (MQ=255)
                                                                              gCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaacaac                   >  1:4533246/1‑101 (MQ=255)
                                                                               cATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAAcc                  >  2:6177436/1‑101 (MQ=255)
                                                                                aTGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAAccc                 >  1:3031185/1‑101 (MQ=255)
                                                                                aTGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAAccc                 >  1:854463/1‑101 (MQ=255)
                                                                                 tGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCg                >  2:4891692/1‑101 (MQ=255)
                                                                                    cGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCAt             <  2:4381759/101‑1 (MQ=255)
                                                                                     gATGCTCTGTGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATg            <  2:2403940/101‑1 (MQ=255)
                                                                                     gATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATg            >  2:1302952/1‑101 (MQ=255)
                                                                                     gATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATg            >  2:6656106/1‑101 (MQ=255)
                                                                                     gATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAAAACAAGCCGCATg            <  1:5532105/101‑1 (MQ=255)
                                                                                      aTGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGa           >  2:6444332/1‑101 (MQ=255)
                                                                                      aTGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGa           >  1:810280/1‑101 (MQ=255)
                                                                                       tGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGAc          <  1:167111/101‑1 (MQ=255)
                                                                                       tGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGAc          >  1:6336280/1‑101 (MQ=255)
                                                                                        gCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGAct         >  2:1156151/1‑101 (MQ=255)
                                                                                        gCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGAct         <  2:888070/101‑1 (MQ=255)
                                                                                         ctctGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGActt        >  1:4037483/1‑101 (MQ=255)
                                                                                          tctGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGActtc       >  1:2820460/1‑101 (MQ=255)
                                                                                           ctGCGTCACCCACGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACGACCCGCATGActtct      >  2:5203155/1‑101 (MQ=255)
                                                                                            tGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGActtctt     >  1:3877219/1‑101 (MQ=255)
                                                                                             gCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACttcttc    <  1:5793550/101‑1 (MQ=255)
                                                                                              cGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTcc   <  1:4156446/101‑1 (MQ=255)
                                                                                              cGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTcc   <  1:5185776/101‑1 (MQ=255)
                                                                                              cGTCACCCATGAGATGGGCTTTGCCCAGGCGCAAGCGAACCGGGTGATCTTCATGGCCGACGCGCAGATCGTCGAGCAGAACAACCCCCACGACCTCTTcc   <  1:2570223/101‑1 (MQ=37)
                                                                                               gTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTcca  >  2:6709875/1‑100 (MQ=255)
                                                                                                  |                                                                                                 
TCGACGAACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCGCCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCG  >  NC_014034/367188‑367383

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 32 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG