Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,479 G→A 2.8% intergenic (+82/+66) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4790G→A 2.8% 13.9 ‑4.2 ‑0.1316intergenic (+82/+66)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (8/1):  ref base (140/166):  total (148/168)
Fisher's exact test strand distribution p-value = 1.44e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.45e-01

Only 100 of 338 total aligned reads displayed.

GCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGT  >  NC_014034/432380‑432579
                                                                                                    |                                                                                                    
gcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCg                                                                                                      <  1:2083837/101‑1 (MQ=255)
  ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGtt                                                                                                    <  2:1473098/101‑1 (MQ=255)
  ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGtt                                                                                                    <  2:2766922/101‑1 (MQ=255)
  ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGtt                                                                                                    <  1:2253859/101‑1 (MQ=255)
   gcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                                                                   <  2:5222656/101‑1 (MQ=255)
    cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTc                                                                                                  <  2:4234312/101‑1 (MQ=255)
    cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTCCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCAGGGCTTCGTTTc                                                                                                  >  1:7065343/1‑101 (MQ=21)
     gggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCa                                                                                                 >  2:51209/1‑101 (MQ=255)
     gggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCa                                                                                                 >  2:4075316/1‑101 (MQ=255)
      ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                                >  1:385934/1‑101 (MQ=255)
       gCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATc                                                                                               <  1:4654838/101‑1 (MQ=255)
        ccACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCt                                                                                              >  1:6420532/1‑101 (MQ=255)
         cACGCCTGGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                             <  1:3305533/101‑1 (MQ=255)
         cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                             <  2:5793995/101‑1 (MQ=255)
         cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                             <  1:1133999/101‑1 (MQ=255)
         cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACGCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCTTTTCATCtt                                                                                             <  2:4313309/101‑1 (MQ=21)
          ccGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTg                                                                                            <  2:4717329/100‑1 (MQ=255)
          aCGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTg                                                                                            >  1:4343428/1‑101 (MQ=255)
              cgggggCGGGGCGGGGGGGCCCTGCCCCCGTC.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCa                                                                                       <  2:1244672/97‑1 (MQ=18)
              cTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                                                                        <  2:4375373/101‑1 (MQ=255)
              cTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                                                                        >  1:1034103/1‑101 (MQ=255)
               tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCa                                                                                       <  2:1339180/101‑1 (MQ=255)
                 aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGAAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCaaa                                                                                     >  2:842437/1‑101 (MQ=255)
                  ggCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAat                                                                                    <  1:6193763/101‑1 (MQ=255)
                  ggCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAat                                                                                    <  2:1962279/101‑1 (MQ=255)
                      gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATcc                                                                                <  1:6238391/101‑1 (MQ=255)
                      gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATcc                                                                                >  1:4457946/1‑101 (MQ=255)
                       ggacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAtt                                                                    >  1:1777315/5‑101 (MQ=2)
                        gCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCg                                                                              <  2:1641303/101‑1 (MQ=255)
                        gCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGACGATGCGGGCTTGGTTTCATCTTGCCCCAAAAATCCCg                                                                              >  1:5707742/1‑101 (MQ=18)
                                gcgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGaa                                                                      <  2:2432212/101‑1 (MQ=255)
                                 cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAt                                                                     <  2:2062023/101‑1 (MQ=255)
                                 cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAt                                                                     <  1:5260477/101‑1 (MQ=255)
                                  gcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtt                                                                    <  1:6680936/101‑1 (MQ=255)
                                   cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAttt                                                                   <  2:268805/101‑1 (MQ=255)
                                   cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCAGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAttt                                                                   <  2:4977474/101‑1 (MQ=255)
                                    tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                                                                  <  1:2942537/101‑1 (MQ=255)
                                    tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                                                                  >  1:58592/1‑101 (MQ=255)
                                       cccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGGGAATTTTGgc                                                               >  1:362719/1‑101 (MQ=255)
                                        ccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGgcg                                                              <  2:1509722/101‑1 (MQ=255)
                                         cccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGgcgc                                                             <  1:3902278/101‑1 (MQ=255)
                                          ccgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCa                                                            >  2:3418033/6‑101 (MQ=255)
                                           cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAg                                                           <  2:3607320/97‑1 (MQ=255)
                                           cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAg                                                           <  1:1256453/97‑1 (MQ=255)
                                           cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAg                                                           <  1:1187970/97‑1 (MQ=255)
                                            gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                                          >  2:6988643/4‑101 (MQ=255)
                                            gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                                          >  1:2350641/4‑101 (MQ=255)
                                             tccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGcc                                                         <  2:5988970/99‑1 (MQ=255)
                                              ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                        <  2:5649765/100‑1 (MQ=255)
                                                 gCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaaa                                                     <  1:3184810/101‑1 (MQ=255)
                                                   ggagtgcccccGGGAGATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGGATTTTGGCGCAGCCAAAaga                                                   >  1:6855924/7‑101 (MQ=37)
                                                   ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga                                                   >  2:6229572/1‑101 (MQ=255)
                                                     aCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                                                 >  1:3493715/1‑101 (MQ=255)
                                                      cTCCCCCCGGGATTTTGGAGGCAAGATGAAAGCCGCCCCGGCCTTGGTTTCATCTGGCCCCAAATACCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAggg                                                <  1:3425488/101‑1 (MQ=21)
                                                      cTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAggg                                                <  1:271783/101‑1 (MQ=255)
                                                       tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGNCGCAGCCAAAAGAgggg                                               >  2:1025565/1‑101 (MQ=255)
                                                         cccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGc                                             <  2:5328070/101‑1 (MQ=255)
                                                         cccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGc                                             <  2:3233212/101‑1 (MQ=255)
                                                         cccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGc                                             <  2:3938099/101‑1 (MQ=255)
                                                         cccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGc                                             <  1:6564148/101‑1 (MQ=255)
                                                             cgtgATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                                         <  1:488079/98‑1 (MQ=255)
                                                             cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                                         <  1:603128/101‑1 (MQ=255)
                                                                gATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                      >  2:3199359/1‑101 (MQ=255)
                                                                 atatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                                     >  1:136947/1‑101 (MQ=255)
                                                                 atatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                                     <  2:3744677/101‑1 (MQ=255)
                                                                 atatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                                     <  2:6435225/101‑1 (MQ=255)
                                                                  tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                                    <  1:3724660/101‑1 (MQ=255)
                                                                   atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                   >  2:258109/1‑101 (MQ=255)
                                                                   atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                   <  2:1812138/101‑1 (MQ=255)
                                                                    tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                                  >  1:1439310/1‑101 (MQ=255)
                                                                       gAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcc                               <  2:1202269/101‑1 (MQ=255)
                                                                        agtcAAGATGAAAGCCGATCCGTGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc                              <  2:7088305/98‑1 (MQ=255)
                                                                        aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc                              <  2:1423097/101‑1 (MQ=255)
                                                                         ggCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                             <  1:4984658/101‑1 (MQ=255)
                                                                         ggCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                             >  1:2515156/1‑101 (MQ=255)
                                                                          gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                            >  2:345792/1‑101 (MQ=255)
                                                                            aaGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcg                          >  1:5477887/1‑101 (MQ=255)
                                                                              gATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggc                        >  1:2763067/1‑101 (MQ=255)
                                                                               aTGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcg                       >  1:2684010/1‑101 (MQ=255)
                                                                                 gAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg                     >  2:3084889/1‑101 (MQ=255)
                                                                                   aaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc                   <  1:2016046/101‑1 (MQ=255)
                                                                                   aaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc                   <  2:2938905/101‑1 (MQ=255)
                                                                                    aGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcg                  >  1:1599108/1‑101 (MQ=255)
                                                                                        gATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgc              >  1:2098716/1‑101 (MQ=255)
                                                                                        gATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgc              >  1:4207424/1‑101 (MQ=255)
                                                                                        gATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgc              >  2:4498270/1‑101 (MQ=255)
                                                                                         aTCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgcg             <  1:80708/101‑1 (MQ=255)
                                                                                          tCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGcggcgcggcggcgcgg            >  1:154095/1‑101 (MQ=255)
                                                                                            cGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGgcgcggcggcgcggcg          >  2:2194894/1‑101 (MQ=255)
                                                                                              ggCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCTGCGGCGCGGCGGCGCGGCGCa        >  2:5721187/1‑101 (MQ=255)
                                                                                              ggCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCa        >  2:6498702/1‑101 (MQ=255)
                                                                                                cTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGc      <  2:3677184/101‑1 (MQ=255)
                                                                                                 ttCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGcc     <  2:2332418/101‑1 (MQ=255)
                                                                                                   cGTTTCATCTTGCCCGAAATACCCGGGGGGGGAATGTTGGCGCAGCC.AAAGGCGGGGCACCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGt  <  1:258113/101‑1 (MQ=37)
                                                                                                   cGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCgg   >  1:4827383/1‑101 (MQ=255)
                                                                                                   cGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGAAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGTCCAGCCgg   >  2:5446649/1‑101 (MQ=255)
                                                                                                    gTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGt  >  1:5181578/1‑101 (MQ=255)
                                                                                                    gTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGt  >  1:5342047/1‑101 (MQ=255)
                                                                                                    gTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGt  >  2:6688058/1‑101 (MQ=255)
                                                                                                    gTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGt  >  1:2862886/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
GCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGT  >  NC_014034/432380‑432579

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG