Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,503 G→A 3.0% intergenic (+106/+42) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,5030G→A 3.0% 14.0 ‑2.5 ‑0.2302intergenic (+106/+42)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (8/1):  ref base (165/128):  total (173/129)
Fisher's exact test strand distribution p-value = 8.34e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.87e-01

Only 100 of 322 total aligned reads displayed.

GGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGCCCAAGCCCGG  >  NC_014034/432403‑432603
                                                                                                     |                                                                                                    
ggacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAtt                                                                                            >  2:2802041/5‑101 (MQ=2)
 gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttg                                                                                           >  2:5388246/4‑100 (MQ=2)
 gCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCg                                                                                                      <  2:1641303/101‑1 (MQ=255)
 gCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGACGATGCGGGCTTGGTTTCATCTTGCCCCAAAAATCCCg                                                                                                      >  1:5707742/1‑101 (MQ=18)
    ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgggg                                                                                                   >  1:1045262/1‑101 (MQ=255)
    ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgggg                                                                                                   <  2:3220893/101‑1 (MQ=255)
    ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgggg                                                                                                   >  1:3771712/1‑101 (MQ=255)
     gggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggggg                                                                                                  >  2:1792125/1‑101 (MQ=255)
      ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGt                                                                                                 >  1:3203875/1‑101 (MQ=255)
        ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGa                                                                                               <  2:7113470/101‑1 (MQ=255)
        ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGa                                                                                               >  1:2587183/1‑101 (MQ=255)
         gcgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGaa                                                                                              >  2:120454/1‑101 (MQ=255)
          cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAt                                                                                             <  1:5260477/101‑1 (MQ=255)
          cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAt                                                                                             <  1:5351614/101‑1 (MQ=255)
           gcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtt                                                                                            <  2:2190941/101‑1 (MQ=255)
            cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCAGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAttt                                                                                           <  2:4977474/101‑1 (MQ=255)
             tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                                                                                          <  1:6606134/101‑1 (MQ=255)
             tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                                                                                          <  2:4503924/101‑1 (MQ=255)
             tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                                                                                          <  1:2942537/101‑1 (MQ=255)
              gCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTg                                                                                         <  2:264389/101‑1 (MQ=255)
                cccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGgc                                                                                       <  2:6134470/101‑1 (MQ=255)
                cccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGGGAATTTTGgc                                                                                       >  1:362719/1‑101 (MQ=255)
                   ccgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCa                                                                                    >  2:3418033/6‑101 (MQ=255)
                    cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAg                                                                                   <  2:2983163/97‑1 (MQ=255)
                     gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                                                                  <  2:4692316/98‑1 (MQ=255)
                     gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                                                                  <  2:1079225/98‑1 (MQ=255)
                     gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                                                                  >  2:1447982/4‑101 (MQ=255)
                       ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGTCCCAAATATCCCGGGGGTGAGTTTTGGCGCAGCCa                                                                                >  1:1836175/2‑101 (MQ=255)
                       ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                                                <  2:5649765/100‑1 (MQ=255)
                          gCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaaa                                                                             <  2:3684352/101‑1 (MQ=255)
                             gACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                                                          <  2:5173786/101‑1 (MQ=255)
                               cTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAggg                                                                        >  2:2402280/1‑101 (MQ=255)
                               cTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAggg                                                                        <  1:271783/101‑1 (MQ=255)
                                  cccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGc                                                                     <  2:3233212/101‑1 (MQ=255)
                                     ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGTCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                                                                  <  2:3673764/101‑1 (MQ=255)
                                      atctatatTTGAGGCAAGATGAAACCCGATCCGGGCTTCCTTTCATCTTTCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                                                                 <  1:2289289/97‑1 (MQ=25)
                                       gggATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcgc                                                                >  1:3427447/1‑101 (MQ=255)
                                       gggATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcgc                                                                >  2:2521005/1‑101 (MQ=255)
                                          atatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcgagat                                                             >  2:2289194/1‑97 (MQ=255)
                                          atatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                                                             <  1:4655893/101‑1 (MQ=255)
                                          atatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                                                             <  2:1477262/101‑1 (MQ=255)
                                           tatTTGAGGCAAGATGAAAGCCGATCCGTGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTTAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                                                            <  2:635938/101‑1 (MQ=255)
                                            atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                                           >  2:394555/1‑101 (MQ=255)
                                            atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                                           <  1:1361751/101‑1 (MQ=255)
                                            atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                                           <  1:1245690/101‑1 (MQ=255)
                                             tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                                                          >  1:1439310/1‑101 (MQ=255)
                                             tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                                                          <  2:4095285/101‑1 (MQ=255)
                                               tGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATc                                                        >  1:1552361/1‑101 (MQ=255)
                                                  ggCAAGATGAAAGCCGATCCGGGCTTCGTTTGATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                                                     <  2:2004716/101‑1 (MQ=255)
                                                  ggCAAGATGAAAGCCGATCCGGGCTTCGTTTCCTCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                                                     <  2:5254150/101‑1 (MQ=255)
                                                  ggCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                                                     >  1:2515156/1‑101 (MQ=255)
                                                   gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                                                    >  2:345792/1‑101 (MQ=255)
                                                    cAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgc                                                   <  2:27076/101‑1 (MQ=255)
                                                     aaGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcg                                                  >  1:5477887/1‑101 (MQ=255)
                                                     aaGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcg                                                  >  2:7055532/1‑101 (MQ=255)
                                                      aGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcgg                                                 >  1:1162882/1‑101 (MQ=255)
                                                       gATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggc                                                >  2:3527845/1‑101 (MQ=255)
                                                       gATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggc                                                >  1:2763067/1‑101 (MQ=255)
                                                        aTGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcg                                               >  1:6095391/1‑101 (MQ=255)
                                                          gAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg                                             >  2:433789/1‑101 (MQ=255)
                                                          gAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg                                             >  2:3084889/1‑101 (MQ=255)
                                                          gAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg                                             >  1:2557988/1‑101 (MQ=255)
                                                            aaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc                                           <  1:2016046/101‑1 (MQ=255)
                                                            aaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc                                           >  2:678034/1‑101 (MQ=255)
                                                                cGATCCGGGCTTCGTTTCATCTTGCCCCAAATAGCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcg                                       >  1:5006487/1‑101 (MQ=255)
                                                                 gATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgc                                      >  1:2098716/1‑101 (MQ=255)
                                                                  aTCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgcg                                     >  2:5454451/1‑101 (MQ=255)
                                                                  aTCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgcg                                     <  1:80708/101‑1 (MQ=255)
                                                                   tCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGcggcgcggcggcgcgg                                    >  1:154095/1‑101 (MQ=255)
                                                                       ggCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCTGCGGCGCGGCGGCGCGGCGCa                                >  2:5721187/1‑101 (MQ=255)
                                                                       ggCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCa                                >  2:6498702/1‑101 (MQ=255)
                                                                        gCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCGGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAg                               <  1:3836408/101‑1 (MQ=255)
                                                                            cGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCgg                           >  1:6821145/1‑101 (MQ=255)
                                                                            cGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCgg                           >  1:209107/1‑101 (MQ=255)
                                                                            cGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCgg                           >  1:4827383/1‑101 (MQ=255)
                                                                            cGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCgg                           <  1:4011741/101‑1 (MQ=255)
                                                                             gTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGt                          >  1:5181578/1‑101 (MQ=255)
                                                                             gTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGt                          >  1:2381513/1‑101 (MQ=255)
                                                                               ttCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCg                        >  1:4460496/1‑101 (MQ=255)
                                                                                   tCTTGCCCCAAATATCCCGGGGGTGAATTTTGTCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGa                    <  1:3546995/101‑1 (MQ=255)
                                                                                   tCTTGCCCCAAAGATCCCGGGGGTGAATGTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGa                    >  1:6269950/1‑101 (MQ=255)
                                                                                   tCTGGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGCGGCGGCGCAGCCGGTCGTTGa                    >  1:5606955/1‑101 (MQ=255)
                                                                                     ttGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATc                  >  2:6862456/1‑101 (MQ=255)
                                                                                      tGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATcg                 <  1:928256/101‑1 (MQ=255)
                                                                                      tGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATcg                 >  2:1543110/1‑101 (MQ=255)
                                                                                        ccccAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATcgcg               >  1:6478452/1‑101 (MQ=255)
                                                                                         cccAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATcgcgc              >  1:2916111/1‑101 (MQ=255)
                                                                                         cccAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATcgcgc              >  1:1747554/1‑101 (MQ=255)
                                                                                            aaaTATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGcc           >  1:418668/1‑101 (MQ=255)
                                                                                            aaaTATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGcc           >  2:6008719/1‑101 (MQ=255)
                                                                                             aaTATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGccc          >  1:4343331/1‑101 (MQ=255)
                                                                                             aaTATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGccc          >  1:4906754/1‑101 (MQ=255)
                                                                                              atatCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGCCCa         >  2:593635/1‑101 (MQ=255)
                                                                                                atCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGCCCAAg       >  2:3827401/1‑101 (MQ=255)
                                                                                                 tCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCCGCCGGGCGTTGATCGCGCGCCCAAGc      >  1:3171778/1‑101 (MQ=255)
                                                                                                   ccGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGCCCAAGccc    <  1:5816718/101‑1 (MQ=255)
                                                                                                   ccGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCCGCGCAGCCGGTCGTTGATCGCGCGCCCAAGccc    <  1:6229891/101‑1 (MQ=255)
                                                                                                     gggggTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGCCCAAGCCCgg  <  1:5910500/101‑1 (MQ=255)
                                                                                                     gggggTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGCCCAAGCCCgg  >  2:442015/1‑101 (MQ=255)
                                                                                                     gggggTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGAGCGGCGCAGCCGGTCGTTGATCGCGCGCCCAAGCCCgg  >  1:6921939/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
GGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGCCCAAGCCCGG  >  NC_014034/432403‑432603

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG