Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 441,868 T→G 100% V329G (GTC→GGC)  gabD → succinate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034441,8680T→G100.0% 1313.3 351V329G (GTC→GGC) gabDsuccinate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (169/182):  ref base (0/0):  total (169/182)

Only 100 of 376 total aligned reads displayed.

CCAGACCTGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGTCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTGGCGGGCGGC  >  NC_014034/441770‑441965
                                                                                                  |                                                                                                 
 cagccgtGCGTCTGCACGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTACGCAAGCTCAATGTCGGCGa                                                                                                >  1:6854589/7‑101 (MQ=255)
 cAGACCTGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGa                                                                                                >  2:5531377/1‑101 (MQ=255)
        gCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTg                                                                                         <  1:2691498/101‑1 (MQ=255)
              gcgcGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGAc                                                                                   <  2:6625502/101‑1 (MQ=255)
               gccgAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACg                                                                                  <  2:417180/99‑1 (MQ=255)
               cgcgAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACg                                                                                  <  1:3764320/101‑1 (MQ=255)
                 cgAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGc                                                                                >  1:3967985/1‑101 (MQ=255)
                  gAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGAAGGCg                                                                               >  2:3499049/1‑101 (MQ=255)
                   aaCCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGGCGGCgg                                                                              >  1:4777741/1‑100 (MQ=255)
                    aCCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTg                                                                             <  2:2610704/101‑1 (MQ=255)
                     ccGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGa                                                                            <  2:841519/101‑1 (MQ=255)
                     ccGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGa                                                                            <  2:722608/101‑1 (MQ=255)
                       ggATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGacc                                                                          >  1:3464603/1‑101 (MQ=255)
                       ggATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGacc                                                                          >  1:1930420/1‑101 (MQ=255)
                       ggATCTAGGTGC.GGGGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGacca                                                                         <  1:6421125/101‑1 (MQ=255)
                         aTCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGcccac                                                                        >  1:3080811/1‑101 (MQ=255)
                          tCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGaccacc                                                                       <  2:4495368/101‑1 (MQ=255)
                          tCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGaccacc                                                                       <  1:2944999/101‑1 (MQ=255)
                          tCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGaccacc                                                                       <  1:1728184/101‑1 (MQ=255)
                           cTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGGGACCACCg                                                                      >  1:1802471/1‑101 (MQ=255)
                             aTGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGGCGGCGTGACCACCcgg                                                                    >  2:7056501/1‑101 (MQ=255)
                             aTGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCggg                                                                    <  1:4080478/101‑1 (MQ=255)
                             aTGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGGGACCACCggg                                                                    >  1:509140/1‑101 (MQ=255)
                              tgtgCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGc                                                                   >  1:705495/1‑101 (MQ=255)
                               gtgCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGcc                                                                  <  1:6585538/101‑1 (MQ=255)
                               gtgCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGGGACCACCGGGcc                                                                  >  2:1320448/1‑101 (MQ=255)
                                tgCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGccc                                                                 <  2:3365244/101‑1 (MQ=255)
                                tgCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGccc                                                                 >  2:2048295/1‑101 (MQ=255)
                                  cAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCtt                                                               <  2:1075740/101‑1 (MQ=255)
                                  cAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCtt                                                               <  2:1662203/101‑1 (MQ=255)
                                    ggcgggcgTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGa                                                             <  1:1043157/101‑1 (MQ=255)
                                     gcgggcgTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGAt                                                            <  2:4371465/101‑1 (MQ=255)
                                     gcgggcgTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGAt                                                            <  1:1966856/101‑1 (MQ=255)
                                     gcgggcgTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGAt                                                            <  2:5377880/101‑1 (MQ=255)
                                       gggcgTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGAtct                                                          >  1:5893734/1‑101 (MQ=255)
                                        ggcgTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGAtctc                                                         <  1:1506761/101‑1 (MQ=255)
                                        ggcgTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGGGACCACCGGGCCCTTGAtctg                                                         >  1:5373261/1‑100 (MQ=255)
                                         gcgTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCg                                                        <  1:1245823/101‑1 (MQ=255)
                                         gcgTCTATGACACGTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCg                                                        <  2:3082868/101‑1 (MQ=255)
                                         gacgctctGACACTGTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCg                                                        <  2:2296406/94‑1 (MQ=255)
                                          cgTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCgg                                                       <  2:5938856/101‑1 (MQ=255)
                                            tCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGcc                                                     >  2:6988013/1‑101 (MQ=255)
                                              tATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGa                                                   >  2:3790506/1‑101 (MQ=255)
                                              tATGACACTTTCGCGGAAAAGCTGGAGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGa                                                   >  2:2790461/1‑101 (MQ=255)
                                               aTGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGAt                                                  <  1:239589/101‑1 (MQ=255)
                                                tGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATg                                                 <  2:6733748/101‑1 (MQ=255)
                                                tGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATg                                                 <  2:5830670/101‑1 (MQ=255)
                                                 gACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGc                                                >  1:6149175/1‑101 (MQ=255)
                                                 gACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGc                                                >  1:826879/1‑101 (MQ=255)
                                                  acacTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCg                                               >  2:5647474/1‑101 (MQ=255)
                                                      tttCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCg                                           <  2:2553385/101‑1 (MQ=255)
                                                         cgcgGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCgaga                                        <  2:1518163/101‑1 (MQ=255)
                                                          gcgGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGACCTTGATCTCGGCCGATGCGGTCGAGaa                                       >  2:4923965/1‑101 (MQ=255)
                                                           cgGAAAGGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAg                                      <  1:3302041/101‑1 (MQ=255)
                                                           cgGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAg                                      >  1:6766122/1‑101 (MQ=255)
                                                           cgGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAg                                      <  2:4827968/101‑1 (MQ=255)
                                                            ggAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAgg                                     <  1:6719503/101‑1 (MQ=255)
                                                             gAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGt                                    <  2:4778098/101‑1 (MQ=255)
                                                             gAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGt                                    <  2:3721491/101‑1 (MQ=255)
                                                             gAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGAGACCACCGGGCCCTTGATCTCGCCCGACGGGGGCgaagagcg                                    >  1:4416895/1‑95 (MQ=255)
                                                               aaaGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCg                                  <  1:5504502/101‑1 (MQ=255)
                                                                aaGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCgg                                 >  1:3492868/1‑100 (MQ=255)
                                                                  gCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAgg                               >  1:95075/1‑101 (MQ=255)
                                                                  gCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAgg                               >  2:5322286/1‑101 (MQ=255)
                                                                   cTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGa                              >  1:3263054/1‑101 (MQ=255)
                                                                   cTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGa                              >  1:4195176/1‑101 (MQ=255)
                                                                    cgggggcgtcggTTCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGACCTTGATCTAGGCCGATGCGGTCGAGAAGGTCGAGGaa                             <  1:5721178/97‑1 (MQ=255)
                                                                      gcggcggcgGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACa                           <  2:6148856/101‑1 (MQ=255)
                                                                      gcggcggcgGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACa                           >  2:781632/1‑101 (MQ=255)
                                                                      gcggcggcgGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACa                           >  1:4669340/1‑101 (MQ=255)
                                                                      gcggcggcgGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACa                           <  1:3031100/101‑1 (MQ=255)
                                                                       cggcggcggTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACat                          <  1:4558225/101‑1 (MQ=255)
                                                                       cggcggcggTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACat                          <  2:2384057/101‑1 (MQ=255)
                                                                        ggcggcggTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACata                         <  2:509128/101‑1 (MQ=255)
                                                                         gcggcggTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACatat                        <  1:4345791/101‑1 (MQ=255)
                                                                          cggcggTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATc                       <  1:4924217/101‑1 (MQ=255)
                                                                            gcggTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGc                     <  2:3251136/101‑1 (MQ=255)
                                                                            gcggTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGc                     <  2:6488867/101‑1 (MQ=255)
                                                                              ggTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCg                   <  1:2149592/101‑1 (MQ=255)
                                                                               gTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGa                  >  1:1344773/1‑101 (MQ=255)
                                                                               gTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGGCGAGGAACATATCGGCGa                  >  2:6920114/1‑101 (MQ=255)
                                                                                  cgcAAGCTCAATGTCGGCGGCGGGCTGAAGGGCGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGAtgt               <  1:6017534/101‑1 (MQ=255)
                                                                                   gcAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGAtgtg              >  1:2557820/1‑101 (MQ=255)
                                                                                   gcAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGAtgtg              >  2:4707215/1‑101 (MQ=255)
                                                                                   gaaaGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGAtgtg              <  1:2820841/99‑1 (MQ=255)
                                                                                     tctcTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATgtggt            <  1:6917878/98‑1 (MQ=255)
                                                                                     aaGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGaagaggg            >  2:3278852/1‑95 (MQ=255)
                                                                                     aaGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATgtggt            >  2:1940508/1‑101 (MQ=255)
                                                                                     aaGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATgtggt            >  1:6518596/1‑101 (MQ=255)
                                                                                     aaGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATgtggg            >  2:2942507/1‑100 (MQ=255)
                                                                                     aaGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGCGCCCTTGATCTCCGCCGATGCGGTCGAGAAGGTCGAGGCACATATCGCCGATggggt            >  2:1165778/1‑101 (MQ=255)
                                                                                      aGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATgtggtg           >  2:1796760/1‑101 (MQ=255)
                                                                                       gCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGtggtgg          <  2:6894873/101‑1 (MQ=255)
                                                                                        cTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTggc         <  1:1602857/101‑1 (MQ=255)
                                                                                          cAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTggcgg       >  2:6097577/1‑101 (MQ=255)
                                                                                          cAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTggcgg       >  1:3137147/1‑101 (MQ=255)
                                                                                          cAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTggcgg       >  2:4020971/1‑101 (MQ=255)
                                                                                           aaTGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTggcggg      <  2:6688460/101‑1 (MQ=255)
                                                                                            aTGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTggcgggc     <  2:3226238/101‑1 (MQ=255)
                                                                                               tcggcgACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTGGCGggcggc  <  1:5313966/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CCAGACCTGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGTCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTGGCGGGCGGC  >  NC_014034/441770‑441965

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG