Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 482,368 G→C 100% G423A (GGC→GCC)  RCAP_rcc00448 → sporulation domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034482,3680G→C100.0% 988.2 265G423A (GGC→GCC) RCAP_rcc00448sporulation domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (119/146):  ref base (0/0):  total (119/146)

Only 100 of 283 total aligned reads displayed.

GGCGTGATGTGGACGAACCAGGTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGGCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCGAGGCCGCAGCCCCCGCCGCG  >  NC_014034/482271‑482465
                                                                                                 |                                                                                                 
 gCGTGATGTGGACGAACCAGGTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCg                                                                                               <  2:5906311/101‑1 (MQ=255)
  cGTGATGTGGACGAACCAGGTGCCGTCGCGGCGGGGGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGa                                                                                              >  1:1602659/1‑101 (MQ=255)
   gatctgtgGACGAACCAGGTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGAc                                                                                             <  1:677511/97‑1 (MQ=255)
   gTGATGTGGACGAACCAGGTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGGGCCGGCGCATCGTTCCGGCCGAGCCGGCTTAAGTCCCCGCCCCCAc                                                                                             >  2:1215965/1‑101 (MQ=255)
   gTGATGTGGACGAACCAGGTGCCGTCGCGGCGGGGGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGAc                                                                                             >  2:81483/1‑101 (MQ=255)
    tGATGTGGACGAACCAGGTGCCGTCGCGGCGGGTGGCCCAACGCACCCCGCAGCCCCGGCGCACCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGGcc                                                                                            >  1:6475632/1‑101 (MQ=255)
    tGATGTGGACGAACCAGGTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGAcc                                                                                            >  1:2865940/1‑101 (MQ=255)
     gATGTGGACGAACCAGGTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCt                                                                                           <  1:2246058/101‑1 (MQ=255)
     gATGTGGACGAACCAGGTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCt                                                                                           >  2:442531/1‑101 (MQ=255)
     gATGTGGACGAACCAGGTGCCGTCGCGGCGGGGGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCt                                                                                           >  2:25720/1‑101 (MQ=255)
     gATGTGGACGAACCAGGTGCCGTCGCGGCGGGGGACCGAACGCACCCCCCAGGGCCCGCGCCTCGTTCCGGCCGAGGCGGCTTACGGCCCCGCCCCgcgcc                                                                                           >  2:3212056/1‑97 (MQ=255)
      aTGTGGACGAACCAGGTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTa                                                                                          <  1:2437185/101‑1 (MQ=255)
      aTGTGGACGAACCAGGTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGGCACCGCCCCGACCTa                                                                                          >  2:4953728/1‑101 (MQ=255)
       tgtgGACGAACCAGGTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTAt                                                                                         <  2:1733371/101‑1 (MQ=255)
       tgtgGACGAACCAGGTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTAt                                                                                         <  2:5808010/101‑1 (MQ=255)
       tgtgGACGAACCAGGTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTAt                                                                                         >  1:6108132/1‑101 (MQ=255)
        gtgGACGAACCAGGTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATc                                                                                        >  2:3062892/1‑101 (MQ=255)
         tgGACGAACCAGGTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCa                                                                                       >  1:1916720/1‑101 (MQ=255)
          ggACGAACCAGGTGCCGTCGCGGCGGGTGCCCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCAt                                                                                      <  1:2294185/101‑1 (MQ=255)
            aCGAACCAGGTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGc                                                                                    <  1:4143824/101‑1 (MQ=255)
              gAACCAGGTGCCGTCGCGGCGGGGGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCCTACGTCACCGCCCCGACCTATCATGccc                                                                                  >  1:5553763/1‑101 (MQ=255)
               aaCCAGGTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGcccc                                                                                 <  1:5997979/101‑1 (MQ=255)
                aCCAGGTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCg                                                                                <  1:4284822/101‑1 (MQ=255)
                aCCAGGTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCg                                                                                <  2:434055/101‑1 (MQ=255)
                  cAGGTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGc                                                                              <  1:2792567/101‑1 (MQ=255)
                   aGGTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGcc                                                                             >  2:6460807/1‑101 (MQ=255)
                    ggTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCt                                                                            <  2:52963/101‑1 (MQ=255)
                    ggTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCt                                                                            <  1:3368098/101‑1 (MQ=255)
                      tGCCGTCGCGGCGGGTGACCGAACGCCCCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTAt                                                                          <  2:6301205/101‑1 (MQ=255)
                      tGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTAt                                                                          <  1:2663384/101‑1 (MQ=255)
                      tGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTAt                                                                          <  1:2556390/101‑1 (MQ=255)
                        ccGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGc                                                                        <  2:6913089/101‑1 (MQ=255)
                         cGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGcc                                                                       <  2:2321742/101‑1 (MQ=255)
                            cgcgGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCcgcg                                                                    >  1:4228395/1‑101 (MQ=255)
                              cggcggGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGcc                                                                  <  2:6643581/101‑1 (MQ=255)
                               ggcggGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGccc                                                                 <  2:2222522/101‑1 (MQ=255)
                               ggcggGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGccc                                                                 >  1:119719/1‑101 (MQ=255)
                                gcggGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCg                                                                >  2:5439829/1‑101 (MQ=255)
                                 cggGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGa                                                               <  1:6976779/101‑1 (MQ=255)
                                   ggTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATg                                                             >  2:6155231/1‑101 (MQ=255)
                                   ggTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATg                                                             <  1:372538/101‑1 (MQ=255)
                                   ggTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCAGACCTATCATGCCCCGGCCTATGCCGCGCCCGATg                                                             >  2:3259278/1‑101 (MQ=255)
                                      gACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCgg                                                          >  2:4713146/1‑101 (MQ=255)
                                      gACCGAACGCAACCCGCAGCGCCGGCGCATCGTTCCGGCCAAACCGGCTTACGTCACCCCCCCGAACTATAATGCCCCGGCATTTGCAGCCTCCGAtacag                                                          >  2:2598729/1‑97 (MQ=255)
                                        ccGACCGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTc                                                        <  1:2963967/101‑1 (MQ=255)
                                          gAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGc                                                      >  2:1805466/1‑101 (MQ=255)
                                           aaCGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGcc                                                     <  1:4820003/101‑1 (MQ=255)
                                           aaCGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGcc                                                     >  2:951854/1‑101 (MQ=255)
                                             cGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGcccc                                                   >  2:274242/1‑101 (MQ=255)
                                             cGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGcccc                                                   <  2:2065418/101‑1 (MQ=255)
                                              gCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCg                                                  >  2:5559156/1‑101 (MQ=255)
                                               cACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGc                                                 >  2:1452767/1‑101 (MQ=255)
                                               cACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGc                                                 >  2:778461/1‑101 (MQ=255)
                                                cccccGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCt                                                <  1:3537812/100‑1 (MQ=255)
                                                  cccGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGc                                              >  2:1124954/1‑101 (MQ=255)
                                                   ccGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCg                                             <  1:6685498/101‑1 (MQ=255)
                                                    cGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCgg                                            >  2:4807408/1‑101 (MQ=255)
                                                      cAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGgt                                          >  1:4578892/1‑101 (MQ=255)
                                                      cAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCCTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGgt                                          <  2:3895606/101‑1 (MQ=255)
                                                      cAGCGCCGGCGCAACGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGgt                                          >  2:1352835/1‑101 (MQ=255)
                                                       aGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGgtg                                         >  1:6100118/1‑101 (MQ=255)
                                                        gcgcCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGgtgt                                        >  2:3597862/1‑101 (MQ=255)
                                                        gcgcCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGgtgc                                        >  2:4305083/1‑100 (MQ=255)
                                                        gcgcCGGCGCATCGTACCGGCCGAGCCGGCTTCCGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGgtgt                                        <  2:5486488/101‑1 (MQ=255)
                                                          gcCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTgtcg                                      <  1:1737183/101‑1 (MQ=255)
                                                           ccGGCGCATCGTTCGGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTgtcgt                                     <  1:5302741/101‑1 (MQ=255)
                                                            cGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTgtcgtc                                    <  1:7092195/101‑1 (MQ=255)
                                                            cGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTgtcgtc                                    <  2:6837010/101‑1 (MQ=255)
                                                             ggCTCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGtcgtcg                                   <  1:661870/101‑1 (MQ=255)
                                                             ggCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGtcgtcg                                   <  2:4151857/101‑1 (MQ=255)
                                                             ggCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCAGCGCCCGATGCGGTCGCCCCGCTGCGGGTGtcgtcg                                   >  2:3469652/1‑101 (MQ=255)
                                                              gcgcATCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGa                                  <  1:1145698/101‑1 (MQ=255)
                                                                  aTCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATc                              <  1:3844546/101‑1 (MQ=255)
                                                                  aTCGTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATc                              <  1:5480344/101‑1 (MQ=255)
                                                                     gTTCCGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCcgc                           >  2:4082357/1‑101 (MQ=255)
                                                                        ccGGCCGAGCCGGCTTACGTCCCCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGcc                        <  1:6751501/101‑1 (MQ=255)
                                                                        ccGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGcc                        >  2:5171473/1‑101 (MQ=255)
                                                                         cGGCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGccc                       >  2:2774023/1‑101 (MQ=255)
                                                                          ggCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCg                      <  2:6583683/101‑1 (MQ=255)
                                                                          ggCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCg                      <  2:1761858/101‑1 (MQ=255)
                                                                          ggCCGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGCCGAAATCCGCGCCCg                      <  1:2385454/101‑1 (MQ=255)
                                                                            ccGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCGAg                    >  1:397574/1‑101 (MQ=255)
                                                                             cGAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCGAgg                   <  1:4978937/101‑1 (MQ=255)
                                                                              gAGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCGAGGc                  >  2:6000947/1‑101 (MQ=255)
                                                                               aGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCGAGGcc                 <  2:1911736/101‑1 (MQ=255)
                                                                               aGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCGAGGcc                 <  2:2865831/101‑1 (MQ=255)
                                                                               aGCCGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCGAGGcc                 >  1:3096284/1‑101 (MQ=255)
                                                                                  cGGCTTACGTCCCCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCGAGGCCGCa              <  2:1532259/101‑1 (MQ=255)
                                                                                  cGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCGAGGCCGCa              <  1:81484/101‑1 (MQ=255)
                                                                                  cGGCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCGAGGCCGCa              >  1:3571609/1‑101 (MQ=255)
                                                                                    gCTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCGAGGCCGCAGc            >  1:5774213/1‑101 (MQ=255)
                                                                                     cTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCGAGGCCGCAGcc           >  1:4315967/1‑101 (MQ=255)
                                                                                     cTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCGAGGCCGCAGcc           <  2:7045091/101‑1 (MQ=255)
                                                                                     cTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCGAGGCCGCAGcc           >  2:6075378/1‑101 (MQ=255)
                                                                                     cTTACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCGAGGCCGCAGcc           <  1:5203435/101‑1 (MQ=255)
                                                                                       tACGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCGAGGCCGCAGcccc         >  2:330448/1‑101 (MQ=255)
                                                                                         cGTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCGAGGCCGCAGCCCccg       <  1:4803486/101‑1 (MQ=255)
                                                                                          gTCACCGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCGAGGCCGCAGCCCccgc      >  2:4566653/1‑101 (MQ=255)
                                                                                             cccGCCCCGACCTATCATGCCCCGGCCTATGCCGCGGCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCGAGGCCGCAGCCCCcgccgc   <  1:6371850/100‑1 (MQ=255)
                                                                                             cccGCCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCGAGGCCGCAGCCCCcgccgc   <  2:6107820/100‑1 (MQ=255)
                                                                                                 |                                                                                                 
GGCGTGATGTGGACGAACCAGGTGCCGTCGCGGCGGGTGACCGAACGCACCCCGCAGCGCCGGCGCATCGTTCCGGCCGAGCCGGCTTACGTCACCGGCCCGACCTATCATGCCCCGGCCTATGCCGCGCCCGATGCGGTCGCCCCGCTGCGGGTGTCGTCGAAATCCGCGCCCGAGGCCGCAGCCCCCGCCGCG  >  NC_014034/482271‑482465

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG