Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 566,409 A→G 100% F80L (TTC→CTC)  RCAP_rcc00528 ← carboxymuconolactone decarboxylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034566,4090A→G100.0% 1187.9 323F80L (TTC→CTC) RCAP_rcc00528carboxymuconolactone decarboxylase
Reads supporting (aligned to +/- strand):  new base (172/150):  ref base (0/0):  total (173/150)

Only 100 of 332 total aligned reads displayed.

TCGGCGGCAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAATTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGATCCAT  >  NC_014034/566312‑566506
                                                                                                 |                                                                                                 
 cggcggCAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTcc                                                                                               >  1:1933409/1‑101 (MQ=255)
  ggcggcAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCt                                                                                              <  2:24691/101‑1 (MQ=255)
  ggcggcAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATAGCCACGGCGCAGATGTCGGCGAGTTCCt                                                                                              >  1:6180798/1‑101 (MQ=255)
    cggcAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGAGCGCCACGGCGCAGATGTCGGCGAGTTCCTcg                                                                                            >  1:4558871/1‑101 (MQ=255)
      gcAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTcgcn                                                                                          >  1:3651839/1‑100 (MQ=255)
       cAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTcgcgc                                                                                         >  2:1075215/1‑101 (MQ=255)
        agtttCGCCCCGGCAGGCCAGGGCGGGGCCCGCATACATCACCGCGGGCCCGCCGCCCATCTGAACCGCCCGGGCGCAGATGTCGGCGAGTTCCTcgcgcg                                                                                        <  2:3146052/98‑1 (MQ=255)
        aaGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTcgcgcg                                                                                        >  1:1227008/1‑101 (MQ=255)
        aaGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTcgcgcg                                                                                        >  1:1466271/1‑101 (MQ=255)
        aaGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTcgcgcc                                                                                        >  2:4042457/1‑100 (MQ=255)
           ttCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTgc                                                                                     >  1:5881286/1‑101 (MQ=255)
            tCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTgcc                                                                                    <  1:5363543/101‑1 (MQ=255)
            tCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTgcc                                                                                    <  2:6074548/101‑1 (MQ=255)
             cGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTgccg                                                                                   <  1:4842341/101‑1 (MQ=255)
               tCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTgccgcc                                                                                 <  1:5817015/101‑1 (MQ=255)
                cccAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGccgccc                                                                                <  2:3001390/101‑1 (MQ=255)
                cccAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGccgccc                                                                                <  2:1643322/101‑1 (MQ=255)
                cccAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGccgccc                                                                                <  2:4356749/101‑1 (MQ=255)
                 ccAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGccgcccg                                                                               <  1:3581689/101‑1 (MQ=255)
                 ccAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGccgcccg                                                                               <  2:660399/101‑1 (MQ=255)
                  cagcagGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGccgcccgc                                                                              >  2:2352897/1‑101 (MQ=255)
                    gcagGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCcgcgc                                                                            >  2:4105010/1‑101 (MQ=255)
                    gcagGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCcgcgc                                                                            <  2:718768/101‑1 (MQ=255)
                          cAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGcatca                                                                      <  2:3356360/101‑1 (MQ=255)
                          cAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGcatca                                                                      >  2:2718137/1‑101 (MQ=255)
                           aaGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGcatcat                                                                     >  1:3186922/1‑101 (MQ=255)
                           aaGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGcatcat                                                                     <  2:4716520/101‑1 (MQ=255)
                            aGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCatcatc                                                                    >  2:4973380/1‑101 (MQ=255)
                             ggCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATcg                                                                   >  1:4183184/1‑101 (MQ=255)
                              gCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATcgc                                                                  >  2:2179097/1‑101 (MQ=255)
                               cATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATcgcg                                                                 >  2:1233357/1‑101 (MQ=255)
                                aTGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCGCCGGCGCCTCATcgccc                                                                >  2:6937518/1‑99 (MQ=255)
                                 tGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGcc                                                               >  1:2697386/1‑101 (MQ=255)
                                 tGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGcc                                                               >  2:414484/1‑101 (MQ=255)
                                 tGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGcc                                                               <  2:346959/101‑1 (MQ=255)
                                   ggCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCGTCATCGCGCCGa                                                             >  2:1037838/1‑101 (MQ=255)
                                     ccGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGAtg                                                           <  2:303732/101‑1 (MQ=255)
                                      cGCATACATCATCGCCGGGCCGCCGCGCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGAtgt                                                          <  1:796208/101‑1 (MQ=255)
                                        cATCCATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGAGCATCATCGCGCCGATGTGa                                                        >  1:6848797/1‑101 (MQ=255)
                                        cATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCTCGCGCATCATCGCGCCGATGTGa                                                        <  1:3846570/101‑1 (MQ=255)
                                        cATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGa                                                        <  1:4896570/101‑1 (MQ=255)
                                        cATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGa                                                        <  2:5378099/101‑1 (MQ=255)
                                         aTACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGaa                                                       <  1:1193993/101‑1 (MQ=255)
                                         aTACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGaa                                                       >  2:906840/1‑101 (MQ=255)
                                         aTACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGaa                                                       <  2:6445139/101‑1 (MQ=255)
                                         aTACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGaa                                                       <  1:2640808/101‑1 (MQ=255)
                                         aTACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGaa                                                       >  2:595879/1‑101 (MQ=255)
                                          tACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGaaa                                                      <  1:2179192/101‑1 (MQ=255)
                                           aCATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAg                                                     >  2:3797625/1‑101 (MQ=255)
                                           aCATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAg                                                     >  1:137375/1‑101 (MQ=255)
                                             atcatcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGcc                                                   >  1:936366/1‑101 (MQ=255)
                                              tcatcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCg                                                  <  1:2373638/101‑1 (MQ=255)
                                              tcatcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCg                                                  >  2:1887073/1‑101 (MQ=255)
                                              ccatcGCCGGGCCTCCGCTCATCTGGAACGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCg                                                  <  1:2586284/100‑1 (MQ=255)
                                                atcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGAt                                                >  2:8088/1‑101 (MQ=255)
                                                atcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGAt                                                >  1:4628086/1‑101 (MQ=255)
                                                atcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCATGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGag                                                >  1:4011516/1‑100 (MQ=255)
                                                  cGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGc                                              <  2:5167370/101‑1 (MQ=255)
                                                   gCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCTCGCGCATCATCGCGCCGATGTGAAAGCCGATGCa                                             >  1:3022296/1‑101 (MQ=255)
                                                   gCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCa                                             >  2:636013/1‑101 (MQ=255)
                                                    ccGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAg                                            >  1:1034633/1‑101 (MQ=255)
                                                     cGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAgg                                           >  1:5040623/1‑101 (MQ=255)
                                                      gggCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAggg                                          >  2:5779334/1‑101 (MQ=255)
                                                       ggCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGc                                         >  1:4758894/1‑101 (MQ=255)
                                                         ccgccgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTc                                       <  2:2592787/101‑1 (MQ=255)
                                                         ccgccgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTc                                       <  2:5442273/101‑1 (MQ=255)
                                                         ccgccgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTc                                       >  2:6257284/1‑101 (MQ=255)
                                                          cgccgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCg                                      <  2:442035/101‑1 (MQ=255)
                                                          cgccgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCg                                      <  1:5737312/101‑1 (MQ=255)
                                                          cgccgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCACCGCGCCGATGTGAAAGCCGATGCAGGGCTCg                                      >  1:6345352/1‑101 (MQ=255)
                                                           gccgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGc                                     <  2:1934715/101‑1 (MQ=255)
                                                           gccgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGc                                     >  1:5203182/1‑101 (MQ=255)
                                                           gccgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGc                                     <  1:6022468/101‑1 (MQ=255)
                                                            ccgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCATGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCa                                    <  2:4011341/101‑1 (MQ=255)
                                                             cgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAg                                   <  1:2216016/101‑1 (MQ=255)
                                                               cccATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCaaga                                 >  1:6420365/1‑98 (MQ=255)
                                                                 cATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCt                               <  2:3866305/101‑1 (MQ=255)
                                                                    cTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTgcg                            >  1:2827736/1‑101 (MQ=255)
                                                                      ggATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGcgcg                          >  1:2074867/1‑101 (MQ=255)
                                                                      ggATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGcgcg                          <  2:2558696/101‑1 (MQ=255)
                                                                       gATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGa                         >  1:6674253/1‑101 (MQ=255)
                                                                       gATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGa                         >  1:4433183/1‑101 (MQ=255)
                                                                        aTCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGAt                        <  1:2982813/101‑1 (MQ=255)
                                                                           gCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGcc                     >  2:6109622/1‑101 (MQ=255)
                                                                             cACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCa                   >  2:6887964/1‑101 (MQ=255)
                                                                             cACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCa                   >  2:1344161/1‑101 (MQ=255)
                                                                             cACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCa                   >  2:2061510/1‑101 (MQ=255)
                                                                               cGGCGCAGATGTCCGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATg                 <  2:5544498/101‑1 (MQ=255)
                                                                                  cgcAGATGTCGGCTAGTTCCTCGCGCGAGACGCCCGCTCGAATCATCGCGACGATGTGAAAGCCGATGCAGGGCACGCAGCGCTGCGCGATGCCCATGGcc              <  2:1203101/101‑1 (MQ=255)
                                                                                     aGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCTATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAgc           <  2:281234/101‑1 (MQ=255)
                                                                                     aGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAgc           >  2:4561633/1‑101 (MQ=255)
                                                                                     aGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAgc           >  2:2906657/1‑101 (MQ=255)
                                                                                      gATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAgcg          <  2:1488801/101‑1 (MQ=255)
                                                                                       aTGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAgcgc         >  1:4402142/1‑101 (MQ=255)
                                                                                       aTGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAgcgc         >  2:3573949/1‑101 (MQ=255)
                                                                                       aTGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAgcgc         >  1:2035340/1‑101 (MQ=255)
                                                                                           cGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGATc     >  1:5633488/1‑101 (MQ=255)
                                                                                           cGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGATc     >  1:7099105/1‑101 (MQ=255)
                                                                                             gCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGATCCa   >  1:2453750/1‑101 (MQ=255)
                                                                                             gCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGATCCa   <  1:1750825/101‑1 (MQ=255)
                                                                                                 |                                                                                                 
TCGGCGGCAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAATTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGATCCAT  >  NC_014034/566312‑566506

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG