Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 580,946 A→C 1.4% intergenic (+67/‑304) RCAP_rcc00541 → / → RCAP_rcc00542 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034580,9460A→C 1.4% 3.6 ‑1.0 ‑0.4350intergenic (+67/‑304)RCAP_rcc00541/RCAP_rcc00542hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (164/181):  total (165/185)
Fisher's exact test strand distribution p-value = 3.76e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.22e-01

Only 100 of 375 total aligned reads displayed.

ACCGCGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.AACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCG  >  NC_014034/580847‑581045
                                                                                                    |                                                                                                    
  cgcgcgTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGc                                                                                                   <  1:2760270/101‑1 (MQ=255)
   gcgcgTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGGTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCa                                                                                                  >  1:1182440/1‑101 (MQ=255)
   gcgcgTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCa                                                                                                  <  1:1815667/101‑1 (MQ=255)
    cgcgTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCaa                                                                                                 <  2:3439424/101‑1 (MQ=255)
     gcgTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAg                                                                                                <  1:4243323/101‑1 (MQ=255)
      cgTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGa                                                                                               <  2:3787152/101‑1 (MQ=255)
       gTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGGTATTTGAGGCCAGaa                                                                                              >  2:816490/1‑100 (MQ=255)
       gTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGAGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAt                                                                                              >  2:6521749/1‑101 (MQ=255)
        tGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGGTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGGTATTTGAGGCAAGAT.a                                                                                            >  1:5990318/1‑101 (MQ=255)
        tGACAAGGTGGTCCGCCTCGATTGAGGGTGCGCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.a                                                                                            <  1:5712572/101‑1 (MQ=21)
         gACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCGAGAT.aa                                                                                           <  2:1630989/101‑1 (MQ=255)
         gACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.aa                                                                                           <  2:5869440/101‑1 (MQ=255)
           cAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.aaac                                                                                         <  1:1063741/101‑3 (MQ=255)
             aGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCCAGAT.aaacgg                                                                                       >  2:4111904/1‑97 (MQ=255)
             aGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.aaacgg                                                                                       >  2:2401567/1‑97 (MQ=255)
              ggTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.aaacggg                                                                                      <  1:45868/101‑6 (MQ=255)
               atgtTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGgc                                                                                      <  1:3869588/100‑1 (MQ=255)
                   tCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCt                                                                                  >  2:3734754/1‑101 (MQ=255)
                   tCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGACCCCTCTTTCGGCTGCGCCGAAATTCT.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCt                                                                                  <  1:2881753/101‑1 (MQ=25)
                    ccGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCG.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTa                                                                                 <  2:6997751/101‑1 (MQ=255)
                    ccGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTa                                                                                 >  2:4784664/1‑101 (MQ=255)
                      gCCTCGATTGAGGCGGCCCGGGCAGGGGGCGGTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATc                                                                               >  1:6951334/1‑101 (MQ=255)
                      gCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATc                                                                               <  1:3693634/101‑1 (MQ=255)
                      gCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATc                                                                               <  2:6499998/101‑1 (MQ=255)
                         tCGATTGGGGCGGCCCGGGCAGGGGGCGGTGCCCCCCCTTTCGGCTGCGCCGAAATTCC.CCCCCGGGATATTTGAGGCAAGATAAACGGGGGCCTATCAcc                                                                            >  1:3905906/1‑101 (MQ=37)
                          cGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCt                                                                           <  2:513213/101‑1 (MQ=255)
                          cGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCt                                                                           >  1:7057443/1‑101 (MQ=255)
                           gATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCtt                                                                          >  1:1973630/1‑101 (MQ=255)
                            aTTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTg                                                                         <  2:1831758/101‑1 (MQ=255)
                               gAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTg                                                                      <  1:3156221/101‑1 (MQ=255)
                               gAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTg                                                                      <  2:4730537/101‑1 (MQ=255)
                                 ggcggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATGACCTTGGGGCa                                                                    >  2:2293310/1‑101 (MQ=21)
                                 ggcggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCa                                                                    <  1:549206/101‑1 (MQ=255)
                                      cccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAatat                                                               <  2:446895/101‑1 (MQ=255)
                                      cccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAatat                                                               <  1:1492010/101‑1 (MQ=255)
                                      cccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATAACCTTGGTGCAAatat                                                               >  1:6571676/1‑101 (MQ=255)
                                       ccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATc                                                              <  2:3031470/101‑1 (MQ=255)
                                        cGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATcc                                                             >  2:5627213/1‑101 (MQ=255)
                                          ggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCgg                                                           <  1:6086612/101‑1 (MQ=255)
                                          ggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCgg                                                           <  2:6859292/101‑1 (MQ=255)
                                          ggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCgg                                                           >  1:5153076/1‑101 (MQ=255)
                                           gCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCggg                                                          >  2:3220216/1‑101 (MQ=255)
                                             aGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTTA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCggggg                                                        >  2:2562381/1‑101 (MQ=255)
                                             aGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCggggg                                                        >  2:2361426/1‑101 (MQ=255)
                                              gggggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCgggggg                                                       >  2:5760757/1‑101 (MQ=255)
                                               ggggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCggggggg                                                      >  1:725369/1‑100 (MQ=255)
                                                    gcTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGc                                                 >  1:2706571/1‑101 (MQ=255)
                                                      tGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCg                                               >  1:4175412/1‑101 (MQ=255)
                                                         ccccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCGCCTTGGTGCAAATATCCGGGGGGTGAGGCCGaaa                                            <  1:4506238/101‑1 (MQ=255)
                                                          cccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAg                                           >  2:533432/1‑101 (MQ=255)
                                                            ctctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGcc                                         <  1:4925462/101‑1 (MQ=255)
                                                            ctctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGcc                                         <  2:4279595/101‑1 (MQ=255)
                                                             tctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                        <  2:6617835/101‑1 (MQ=255)
                                                             tctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                        <  2:1911457/101‑1 (MQ=255)
                                                              ctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGa                                       >  2:5948744/1‑101 (MQ=255)
                                                                ttCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgg                                     <  1:767829/101‑1 (MQ=255)
                                                                ttCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgg                                     >  2:6659112/1‑101 (MQ=255)
                                                                  cGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgggg                                   >  1:5642229/1‑101 (MQ=255)
                                                                     cTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCa                                <  1:3432547/101‑1 (MQ=255)
                                                                       ttcggccaaTTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGATGGGGTTTATCTTGCCCCAAATATCCCGGGGGTGAGG.CG.AAGCCGAGGGGGCAgcg                             <  1:2202565/94‑1 (MQ=37)
                                                                       gcgcCGAAATTCC.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCCCCTTGGTGCAAATACCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgc                              <  2:6060511/101‑1 (MQ=38)
                                                                       gcgcCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgc                              >  2:1071219/1‑101 (MQ=255)
                                                                        tcggccaaTTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGATGGGGTTTATCTTGCCCCAAATATCCCGGGGGTGAGG.CG.AAGCCGAGGGGGCAgcgc                            <  2:7075430/95‑1 (MQ=37)
                                                                        cgcCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgcg                             <  1:622198/101‑1 (MQ=255)
                                                                           tgAAATTCA.CCTCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccc                          <  2:3332408/100‑1 (MQ=255)
                                                                           cGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccc                          <  2:2884903/101‑1 (MQ=255)
                                                                            gAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                         >  1:5348592/1‑101 (MQ=255)
                                                                            gAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                         <  2:1452236/101‑1 (MQ=255)
                                                                            gAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                         <  2:3833563/101‑1 (MQ=255)
                                                                             aaaTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc                        >  1:4747355/1‑101 (MQ=255)
                                                                              aaTTCC.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCt                       <  1:5779006/101‑1 (MQ=255)
                                                                              aaTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCt                       <  2:776984/101‑1 (MQ=255)
                                                                               aTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtt                      <  2:2628750/101‑1 (MQ=255)
                                                                                ttCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCttt                     >  2:1759325/1‑101 (MQ=255)
                                                                                 tCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccgcttc                    >  1:3313909/1‑96 (MQ=255)
                                                                                 tCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                    <  1:1656683/101‑1 (MQ=255)
                                                                                 tCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                    >  2:6700321/1‑101 (MQ=255)
                                                                                 tCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                    >  2:2707773/1‑101 (MQ=255)
                                                                                  cA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTc                   >  2:1280410/1‑101 (MQ=255)
                                                                                  cA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATACGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTc                   >  2:5256997/1‑101 (MQ=255)
                                                                                    ccccccGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTcc                  <  2:6412295/100‑1 (MQ=255)
                                                                                      cccgGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcg                <  1:2153540/101‑1 (MQ=255)
                                                                                        ccGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtc              <  1:3565214/101‑1 (MQ=255)
                                                                                        ccGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtc              <  1:3777183/101‑1 (MQ=255)
                                                                                        ccGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtc              >  1:6460898/1‑101 (MQ=255)
                                                                                          gggATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt            >  2:6221625/1‑101 (MQ=255)
                                                                                          gggATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt            >  1:96897/1‑101 (MQ=255)
                                                                                          gggATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt            >  2:3569573/1‑101 (MQ=255)
                                                                                           ggATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtg           >  1:1301125/1‑101 (MQ=255)
                                                                                           ggATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtg           >  2:2093089/1‑101 (MQ=255)
                                                                                           ggATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtg           <  2:22357/101‑1 (MQ=255)
                                                                                            gATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtgt          <  1:6873609/101‑1 (MQ=255)
                                                                                             atatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGtgtg         >  1:5576897/1‑101 (MQ=255)
                                                                                              tatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg        <  2:5073985/101‑1 (MQ=255)
                                                                                              tatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg        >  2:4216908/1‑101 (MQ=255)
                                                                                               atTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGTGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa       >  1:4858302/1‑101 (MQ=255)
                                                                                               atTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa       >  1:5255085/1‑101 (MQ=255)
                                                                                                tttGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGAt      >  1:583624/1‑101 (MQ=255)
                                                                                                tttGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGAt      <  1:171893/101‑1 (MQ=255)
                                                                                                  tGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg    >  2:5481420/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
ACCGCGCGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.AACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCG  >  NC_014034/580847‑581045

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG