Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 580,953 A→G 1.4% intergenic (+74/‑297) RCAP_rcc00541 → / → RCAP_rcc00542 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034580,9530A→G 1.4% 2.3 ‑1.0 ‑0.5342intergenic (+74/‑297)RCAP_rcc00541/RCAP_rcc00542hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (167/170):  total (168/174)
Fisher's exact test strand distribution p-value = 3.72e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.43e-01

Only 100 of 376 total aligned reads displayed.

CGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.AACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAAC  >  NC_014034/580853‑581049
                                                                                                     |                                                                                                  
cgTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGa                                                                                                    <  2:3787152/101‑1 (MQ=255)
 gTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAt                                                                                                   <  1:5576174/101‑1 (MQ=255)
  tGACAAGGTGTTCCGTCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGAGCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.a                                                                                                 <  1:1207195/101‑1 (MQ=255)
  tGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGGTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGGTATTTGAGGCAAGAT.a                                                                                                 >  1:5990318/1‑101 (MQ=255)
  tGACAAGGTGGTCCGCCTCGATTGAGGGTGCGCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.a                                                                                                 <  1:5712572/101‑1 (MQ=21)
   gACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCGAGAT.aa                                                                                                <  2:1630989/101‑1 (MQ=255)
   gACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAACTCA.CCCCCCGGATATTTGAGGCAAGAT.aa                                                                                                >  2:4328333/1‑101 (MQ=255)
    aCAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.aaa                                                                                               >  2:3076493/1‑100 (MQ=255)
     cAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.aaac                                                                                              <  1:1063741/101‑3 (MQ=255)
     cAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.aaac                                                                                              <  1:218761/101‑3 (MQ=255)
      caGGTGTTCCGGCGCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.aaacg                                                                                             <  2:3314585/100‑4 (MQ=255)
      aaGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.aaacg                                                                                             >  2:622178/1‑98 (MQ=255)
        ggTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.aaacggg                                                                                           <  1:45868/101‑6 (MQ=255)
        ggTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.aaaaggg                                                                                           >  2:1329088/1‑96 (MQ=255)
         gtgtTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGgc                                                                                           >  2:5887325/1‑101 (MQ=255)
             tCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGGGGCGCCCCCCCTTTTTGCTTGGCCGGAAATTA.CCCCCCGGGTAATTGAAGGAAAATAAACGGGGGccc                                                                                       >  2:4505999/1‑100 (MQ=255)
             tCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCt                                                                                       >  2:3734754/1‑101 (MQ=255)
              ccGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTa                                                                                      >  2:4784664/1‑101 (MQ=255)
              ccGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTa                                                                                      >  1:979333/1‑101 (MQ=255)
              ccGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTa                                                                                      <  1:4877763/101‑1 (MQ=255)
               cGCCTCGATTGATGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAt                                                                                     <  1:7045306/101‑1 (MQ=255)
               cGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAt                                                                                     <  1:1722675/101‑1 (MQ=255)
                gCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.c                                                                                   <  1:3672500/101‑1 (MQ=255)
                 ccTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGGGCCTAT.Ca                                                                                  >  1:3899497/1‑101 (MQ=255)
                  cTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CAc                                                                                 <  1:424205/101‑1 (MQ=255)
                    cGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCt                                                                               <  1:5835862/101‑1 (MQ=255)
                    cGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCt                                                                               <  2:513213/101‑1 (MQ=255)
                      cttGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTg                                                                             <  1:5467036/100‑1 (MQ=255)
                         gAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGGAAGATAAACGGGCGCCTAT.CACCTTGGTg                                                                          >  2:6980977/1‑101 (MQ=255)
                         gAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTg                                                                          <  1:3156221/101‑1 (MQ=255)
                           ggcggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.GACCTTGGGGCa                                                                        >  2:2293310/1‑101 (MQ=21)
                           ggcggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCa                                                                        <  2:6442237/101‑1 (MQ=255)
                              ggcCCGGGCAGGGGGCGCTGCCCCCTCTTTTGGCCGCGCCGGAATTCA.CCCCCCGGGTATTTTAGGCAAGATAAACGGGCGCCGATCCACCTTGGGCCaaa                                                                      >  2:2390877/1‑101 (MQ=12)
                               gcCCGGGCAGGGGGCGCCTCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAata                                                                    <  1:6923490/101‑1 (MQ=25)
                                cccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.AACCTTGGTGCAAatat                                                                   >  1:6571676/1‑101 (MQ=255)
                                  cGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATcc                                                                 <  2:6014510/101‑1 (MQ=255)
                                  cGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATcc                                                                 >  2:5627213/1‑101 (MQ=255)
                                   gggCCGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCg                                                                <  1:4073559/101‑1 (MQ=255)
                                   gggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCg                                                                <  2:1832939/101‑1 (MQ=255)
                                    ggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCgg                                                               <  1:6086612/101‑1 (MQ=255)
                                    ggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCgg                                                               <  2:6859292/101‑1 (MQ=255)
                                      cAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCgggg                                                             >  2:6537501/1‑101 (MQ=255)
                                      cAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCgggg                                                             >  2:5778704/1‑101 (MQ=255)
                                       aGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTTA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCggggg                                                            >  2:2562381/1‑101 (MQ=255)
                                       aGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCggggg                                                            >  2:2361426/1‑101 (MQ=255)
                                       aGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCggggg                                                            >  1:2844342/1‑101 (MQ=255)
                                         ggggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCggggggg                                                          >  1:725369/1‑100 (MQ=255)
                                          gggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTg                                                         <  1:5738909/101‑1 (MQ=255)
                                           ggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGa                                                        <  1:152622/101‑1 (MQ=255)
                                                tGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCg                                                   <  1:6218052/101‑1 (MQ=255)
                                                tGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCg                                                   <  2:3544788/101‑1 (MQ=255)
                                                 gCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGa                                                  >  1:4790972/1‑101 (MQ=255)
                                                  cccccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGaa                                                 <  1:872895/101‑1 (MQ=255)
                                                  cccccTCTTTCGGCTGCGCCGAAATTCA.CCCCCCGGGTATTTGAGGCAAGATAAACGGGCGCCTAT.CATCTTGGTGCAAATATCCGGGGGGGGAGGCCGaa                                                 >  2:5256342/1‑101 (MQ=37)
                                                   ccccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CGCCTTGGTGCAAATATCCGGGGGGTGAGGCCGaaa                                                <  1:4506238/101‑1 (MQ=255)
                                                   ccccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGaaa                                                <  1:1696471/101‑1 (MQ=255)
                                                    cccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAg                                               >  2:533432/1‑101 (MQ=255)
                                                     ccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGc                                              >  1:2715585/1‑101 (MQ=255)
                                                      ctctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGcc                                             <  1:4925462/101‑1 (MQ=255)
                                                       tctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                            <  2:6617835/101‑1 (MQ=255)
                                                       tctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                            >  1:1321492/1‑101 (MQ=255)
                                                       tctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGGGAGGCCGAAAGCCg                                            >  2:2744974/1‑101 (MQ=255)
                                                         tttCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAg                                          <  2:4605267/101‑1 (MQ=255)
                                                          ttCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgg                                         <  1:767829/101‑1 (MQ=255)
                                                            cGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgggg                                       >  1:5642229/1‑101 (MQ=255)
                                                              gCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGCGGTGAGGCCGAAAGCCGAGGGGGc                                     <  2:3404812/101‑1 (MQ=255)
                                                               cTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCa                                    <  2:6669094/101‑1 (MQ=255)
                                                                cttcggccaaTTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGATGGGGTT.TATCTTGCCGCAAATATCCCGGGGGTGAGG.CG.AAGCCGAGGGGGCAgc                                  >  1:5097686/9‑101 (MQ=25)
                                                                 gcgcCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgc                                  <  1:3734904/101‑1 (MQ=255)
                                                                  tcggccaaTTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGATGGGGTT.TATCTTGCCCCAAATATCCCGGGGGTGAGG.CG.AAGCCGAGGGGGCAgcgc                                <  2:7075430/95‑1 (MQ=37)
                                                                  tcggccaaTTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGATGGGGTT.TATCTTGCCCCAAATATCCCGGGGGTGAGG.CG.AAGCCGAGGGGGCAgcgc                                <  2:6141742/95‑1 (MQ=37)
                                                                      gAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                             >  1:5348592/1‑101 (MQ=255)
                                                                      gAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                             >  2:2171349/1‑101 (MQ=255)
                                                                       aaaTTCC.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc                            <  1:350837/101‑1 (MQ=255)
                                                                       aaaTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc                            >  1:2130001/1‑101 (MQ=255)
                                                                        aaTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCt                           <  2:776984/101‑1 (MQ=255)
                                                                         aTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtt                          <  2:2628750/101‑1 (MQ=255)
                                                                          ttCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCttt                         >  2:1192852/1‑101 (MQ=255)
                                                                          ttCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCttt                         >  2:1759325/1‑101 (MQ=255)
                                                                           tCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                        >  2:6700321/1‑101 (MQ=255)
                                                                            cA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTc                       >  1:6455480/1‑101 (MQ=255)
                                                                             a.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTcc                      >  2:650343/1‑101 (MQ=255)
                                                                                   cGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcg                 >  2:1913376/1‑101 (MQ=255)
                                                                                    gggATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                >  2:3833162/1‑101 (MQ=255)
                                                                                    gggATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                >  2:2934947/1‑101 (MQ=255)
                                                                                     ggATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtg               >  1:2402861/1‑101 (MQ=255)
                                                                                     ggATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtg               >  2:2093089/1‑101 (MQ=255)
                                                                                      gATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtgt              <  1:6873609/101‑1 (MQ=255)
                                                                                       atatTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGtgtg             >  1:3823803/1‑101 (MQ=255)
                                                                                        tatTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg            <  2:5073985/101‑1 (MQ=255)
                                                                                        tatTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg            >  2:4216908/1‑101 (MQ=255)
                                                                                        tatTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg            >  1:1906260/1‑101 (MQ=255)
                                                                                         atTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGTGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa           >  1:3412900/1‑101 (MQ=255)
                                                                                         atTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa           >  2:149602/1‑101 (MQ=255)
                                                                                         atTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa           >  1:5255085/1‑101 (MQ=255)
                                                                                           ttGAGGCAATATAAACGGGCGCCTAT.CGCCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc         <  2:866112/101‑1 (MQ=37)
                                                                                            tGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg        >  2:5481420/1‑101 (MQ=255)
                                                                                            tGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg        >  2:3954175/1‑101 (MQ=255)
                                                                                            tGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg        <  2:2706469/101‑1 (MQ=255)
                                                                                                 cAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCaa   >  1:3251101/1‑101 (MQ=255)
                                                                                                     |                                                                                                  
CGTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.AACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAAC  >  NC_014034/580853‑581049

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG