Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 580,954 T→G 1.6% intergenic (+75/‑296) RCAP_rcc00541 → / → RCAP_rcc00542 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034580,9541A→G 1.6% 2.6 ‑1.6 ‑0.1313intergenic (+75/‑296)RCAP_rcc00541/RCAP_rcc00542hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (160/147):  total (161/152)
Fisher's exact test strand distribution p-value = 2.02e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.17e-01

Only 100 of 372 total aligned reads displayed.

GTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGAT.AA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAAC  >  NC_014034/580854‑581049
                                                                                                    |                                                                                                 
gTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGAGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGAt                                                                                                   >  2:6521749/1‑101 (MQ=255)
 tGACAAGGTGTTCCGTCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGAGCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGAT.a                                                                                                 <  1:1207195/101‑1 (MQ=255)
  gACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGAT.aa                                                                                                <  1:6911398/101‑1 (MQ=255)
  gACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAACTCACCCCCCGGATATTTGAGGCAAGAT.aa                                                                                                >  2:4328333/1‑101 (MQ=255)
    cAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGAT.aaac                                                                                              <  1:1063741/101‑3 (MQ=255)
     aaGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGAT.aaacg                                                                                             >  2:622178/1‑98 (MQ=255)
     aaGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGAT.aaacg                                                                                             <  1:5828575/101‑4 (MQ=255)
        gtgtTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGgc                                                                                          >  2:5887325/1‑101 (MQ=255)
         tgtTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGGGCGGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCCGGATATTTGAGGCAAGAT.aaacggggg                                                                                         >  1:5246840/1‑94 (MQ=38)
          gtTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGgcgc                                                                                        >  1:458192/1‑101 (MQ=255)
            tCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGGGGCGCCCCCCCTTTTTGCTTGGCCGGAAATTACCCCCCGGGTAATTGAAGGAAAATAAA.CGGGGGccc                                                                                      >  2:4505999/1‑100 (MQ=255)
            tCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCt                                                                                      <  2:1907466/101‑1 (MQ=255)
             ccGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTa                                                                                     <  2:6818528/101‑1 (MQ=255)
             ccGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTa                                                                                     >  2:4784664/1‑101 (MQ=255)
              cGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTAt                                                                                    <  2:5952217/101‑1 (MQ=255)
              cGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTAt                                                                                    <  1:1722675/101‑1 (MQ=255)
              cGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTAt                                                                                    <  2:2554565/101‑1 (MQ=255)
               gCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATc                                                                                   <  2:6499998/101‑1 (MQ=255)
                 cTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCAc                                                                                 <  1:424205/101‑1 (MQ=255)
                   cGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCt                                                                               >  2:1654922/1‑101 (MQ=255)
                   cGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCt                                                                               <  1:4047102/101‑1 (MQ=255)
                    gATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCtt                                                                              >  1:2771500/1‑101 (MQ=255)
                     aTTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTTGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.AGGGCGCCTATCACCTTg                                                                             >  2:150335/1‑101 (MQ=21)
                     aTTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTg                                                                             <  2:1831758/101‑1 (MQ=255)
                     aTTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTg                                                                             >  1:7067734/1‑101 (MQ=255)
                        gAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGGAAGATAAA.CGGGCGCCTATCACCTTGGTg                                                                          >  2:6980977/1‑101 (MQ=255)
                          ggcggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCa                                                                        <  2:6442237/101‑1 (MQ=255)
                           gcggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCaa                                                                       <  1:6656496/101‑1 (MQ=255)
                            cggcCCGGGCATGTGGCGCTGCCCCCTCTTTCGGCTGCTCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCaaa                                                                      <  1:3647470/101‑1 (MQ=25)
                             ggcCCGGGCAGGGGGCGGTGCCCCCTCTTTCGGCTGCGCCGAAATTTACCCCCGGGATATTTGAGGCAAGATAAA.CGGGGGCCTATGACCTTTGTGCaaaa                                                                     >  2:5606578/1‑100 (MQ=21)
                               cccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAatat                                                                   <  2:5124827/101‑1 (MQ=255)
                                ccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATc                                                                  <  2:3031470/101‑1 (MQ=255)
                                ccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATc                                                                  >  1:6270301/1‑101 (MQ=255)
                                  gggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCg                                                                <  1:852751/101‑1 (MQ=255)
                                    gCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCggg                                                              >  2:3220216/1‑101 (MQ=255)
                                     cAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCgggg                                                             >  2:5778704/1‑101 (MQ=255)
                                          ggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGa                                                        >  2:2383322/1‑101 (MQ=255)
                                          ggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGa                                                        <  1:152622/101‑1 (MQ=255)
                                           gcgcTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAg                                                       <  1:2161608/101‑1 (MQ=255)
                                               tGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCg                                                   >  2:6698932/1‑101 (MQ=255)
                                                gCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGa                                                  <  1:6053294/101‑1 (MQ=255)
                                                 cccccTCTTTCGGCTGCGCCGAAATTCACCCCCCGGGTATTTGAGGCAAGATAAA.CGGGCGCCTATCATCTTGGTGCAAATATCCGGGGGGGGAGGCCGaa                                                 >  2:5256342/1‑101 (MQ=37)
                                                  ccccTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGGGAGGCCGaaa                                                >  1:3790965/1‑101 (MQ=255)
                                                   cccTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAg                                               >  1:5525322/1‑101 (MQ=255)
                                                   cccTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAg                                               >  1:737834/1‑101 (MQ=255)
                                                   cccTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAg                                               >  2:533432/1‑101 (MQ=255)
                                                    ccTCTTTCGGCTGCGCCGAACTTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGc                                              <  1:5626979/101‑1 (MQ=255)
                                                    ccTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGc                                              >  1:2715585/1‑101 (MQ=255)
                                                      tctTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                            >  1:1321492/1‑101 (MQ=255)
                                                      tctTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                            >  2:6104504/1‑101 (MQ=255)
                                                      tctTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                            <  2:6617835/101‑1 (MQ=255)
                                                      tctTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                            >  1:7000410/1‑101 (MQ=255)
                                                        tttCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAg                                          <  2:4605267/101‑1 (MQ=255)
                                                         ttCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATTACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgg                                         <  2:3461487/101‑1 (MQ=255)
                                                         ttCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgg                                         >  2:6659112/1‑101 (MQ=255)
                                                              cTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCa                                    >  2:2332169/1‑101 (MQ=255)
                                                                gcgcCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgc                                  <  1:3734904/101‑1 (MQ=255)
                                                                gcgcCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGACGGGGCAgc                                  >  1:6212801/1‑101 (MQ=255)
                                                                 cgcCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgcg                                 >  2:2697472/1‑101 (MQ=255)
                                                                      aaaTTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc                            >  1:4747355/1‑101 (MQ=255)
                                                                      aaaTTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc                            <  2:1973558/101‑1 (MQ=255)
                                                                      aaaTTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc                            >  1:2130001/1‑101 (MQ=255)
                                                                      aaaTTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc                            <  1:4907135/101‑1 (MQ=255)
                                                                        aTTCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtt                          >  1:4498828/1‑101 (MQ=255)
                                                                          tCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                        <  2:2744305/101‑1 (MQ=255)
                                                                          tCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                        <  1:3154273/101‑1 (MQ=255)
                                                                          tCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                        >  1:5688527/1‑101 (MQ=255)
                                                                          tCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                        >  1:5718761/1‑101 (MQ=255)
                                                                          tCACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                        <  1:1656683/101‑1 (MQ=255)
                                                                           cACCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGGGAGGCCGAAAGCCGGGGGGCCCGCGCCCCCTTTTc                       >  2:2291003/1‑101 (MQ=37)
                                                                            aCCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTcc                      <  1:5429932/101‑1 (MQ=255)
                                                                             cccccGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTccc                     <  2:2569389/101‑1 (MQ=255)
                                                                               cccGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgt                   <  1:533444/101‑1 (MQ=255)
                                                                                  gggATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                >  2:3833162/1‑101 (MQ=255)
                                                                                  gggATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                >  1:5841033/1‑101 (MQ=255)
                                                                                  gggATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                >  1:96897/1‑101 (MQ=255)
                                                                                   ggATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCTCGTCgtg               >  2:2894084/1‑101 (MQ=255)
                                                                                   ggATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtg               >  1:2402861/1‑101 (MQ=255)
                                                                                   ggATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtg               >  1:6477535/1‑101 (MQ=255)
                                                                                   ggATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtg               <  2:1428394/101‑1 (MQ=255)
                                                                                    gagatTTGAGGCAAGAA.AATCGGGCGCCTATCCCCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtgt              <  2:7074270/98‑1 (MQ=37)
                                                                                    gATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtgg              >  2:1040747/1‑100 (MQ=255)
                                                                                     atatTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGtgtg             <  2:760988/101‑1 (MQ=255)
                                                                                      tatTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg            >  1:1906260/1‑101 (MQ=255)
                                                                                      tatTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg            <  2:5073985/101‑1 (MQ=255)
                                                                                      tatTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg            >  2:4216908/1‑101 (MQ=255)
                                                                                       atTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGTGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa           >  1:4858302/1‑101 (MQ=255)
                                                                                       atTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGTGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa           >  1:3412900/1‑101 (MQ=255)
                                                                                       atTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTggt           >  1:3869592/1‑100 (MQ=255)
                                                                                       atTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa           >  2:3360638/1‑101 (MQ=255)
                                                                                       atTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa           >  2:3570718/1‑101 (MQ=255)
                                                                                         ttGAGGCAATATAAA.CGGGCGCCTATCGCCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc         <  2:866112/101‑1 (MQ=37)
                                                                                         ttGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc         >  1:3437289/1‑101 (MQ=255)
                                                                                          tGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg        >  2:5481420/1‑101 (MQ=255)
                                                                                          tGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg        >  2:3778951/1‑101 (MQ=255)
                                                                                          tGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg        >  2:3954175/1‑101 (MQ=255)
                                                                                              gCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCa    >  2:1255616/1‑101 (MQ=255)
                                                                                               cAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCaa   <  2:2151890/101‑1 (MQ=255)
                                                                                               cAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCaa   >  1:3251101/1‑101 (MQ=255)
                                                                                                aaGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGGGGATCGCGCAAc  <  1:5052555/101‑1 (MQ=255)
                                                                                                    |                                                                                                 
GTGACAAGGTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGAT.AA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAAC  >  NC_014034/580854‑581049

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG