Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 580,960 G→A 1.4% intergenic (+81/‑290) RCAP_rcc00541 → / → RCAP_rcc00542 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034580,9600G→A 1.4% 3.9 ‑1.6 ‑0.2345intergenic (+81/‑290)RCAP_rcc00541/RCAP_rcc00542hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (177/163):  total (178/167)
Fisher's exact test strand distribution p-value = 2.02e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.74e-01

Only 100 of 377 total aligned reads displayed.

GTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGAT.AACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGA  >  NC_014034/580862‑581060
                                                                                                   |                                                                                                    
atgtTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGgc                                                                                                     <  1:3869588/100‑1 (MQ=255)
     ccGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCGCCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTa                                                                                                <  2:6997751/101‑1 (MQ=255)
     ccGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTa                                                                                                <  2:6818528/101‑1 (MQ=255)
      cGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAt                                                                                               <  2:5952217/101‑1 (MQ=255)
         cTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCAc                                                                                            <  1:424205/101‑1 (MQ=255)
          tCGATTGGGGCGGCCCGGGCAGGGGGCGGTGCCCCCCCTTTCGGCTGCGCCGAAATTCCCCCCCGGGATATTTGAGGCAAGATAAACGGGGGCCTATCAcc                                                                                           >  1:3905906/1‑101 (MQ=37)
           cGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCt                                                                                          <  1:5835862/101‑1 (MQ=255)
            gATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCtt                                                                                         >  1:1973630/1‑101 (MQ=255)
            gATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCtt                                                                                         >  1:2771500/1‑101 (MQ=255)
             aTTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTg                                                                                        >  1:7067734/1‑101 (MQ=255)
               tGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGt                                                                                      >  1:1233927/1‑101 (MQ=255)
               tGAGGCGGCCCGGGCAGGGGGCGACGCCCCCTCTTTCGGCTGAGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGt                                                                                      >  2:1642934/1‑101 (MQ=25)
                  ggcggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCa                                                                                   <  1:549206/101‑1 (MQ=255)
                  ggcggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCa                                                                                   <  2:6442237/101‑1 (MQ=255)
                    cggcCCGGGCATGTGGCGCTGCCCCCTCTTTCGGCTGCTCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCaaa                                                                                 <  1:3647470/101‑1 (MQ=25)
                    cggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCaaa                                                                                 <  1:5293613/101‑1 (MQ=255)
                     ggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAat                                                                                >  1:4642581/1‑101 (MQ=255)
                     ggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAat                                                                                <  1:6883253/101‑1 (MQ=255)
                       cccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAatat                                                                              <  2:5124827/101‑1 (MQ=255)
                       cccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAatat                                                                              <  1:1492010/101‑1 (MQ=255)
                        ccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATc                                                                             <  1:239339/101‑1 (MQ=255)
                         cgtgcggggggCGCTTCCCCCTCTTTCGGCTTCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATcc                                                                            <  2:5401593/95‑1 (MQ=21)
                         cGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATcc                                                                            <  1:5923550/101‑1 (MQ=255)
                          gggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCg                                                                           <  1:852751/101‑1 (MQ=255)
                           ggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCgg                                                                          >  1:5153076/1‑101 (MQ=255)
                           ggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCgg                                                                          <  1:6086612/101‑1 (MQ=255)
                             cAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCgggg                                                                        >  2:6537501/1‑101 (MQ=255)
                             cAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCgggg                                                                        >  2:5580496/1‑101 (MQ=255)
                               gggggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCgggggg                                                                      >  1:4822284/1‑101 (MQ=255)
                               gggggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCgggggg                                                                      >  2:5760757/1‑101 (MQ=255)
                                 gggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTg                                                                    <  1:5738909/101‑1 (MQ=255)
                                 gggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTg                                                                    <  2:7088240/101‑1 (MQ=255)
                                  ggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGa                                                                   >  2:2383322/1‑101 (MQ=255)
                                   gcgcTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAg                                                                  >  2:5879302/1‑101 (MQ=255)
                                       tGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCg                                                              <  2:3544788/101‑1 (MQ=255)
                                          ccccTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGaaa                                                           <  1:6522080/101‑1 (MQ=255)
                                           cccTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAg                                                          >  1:737834/1‑101 (MQ=255)
                                              tctTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                                       <  2:3722473/101‑1 (MQ=255)
                                              tctTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                                       <  1:5417353/101‑1 (MQ=255)
                                              tctTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                                       >  2:6626774/1‑101 (MQ=255)
                                                tttCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAg                                                     >  2:1947988/1‑101 (MQ=255)
                                                     gCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGc                                                >  1:7018627/1‑101 (MQ=255)
                                                     gCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGCGGTGAGGCCGAAAGCCGAGGGGGc                                                <  2:3404812/101‑1 (MQ=255)
                                                      cTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCa                                               >  2:2332169/1‑101 (MQ=255)
                                                        gcgcCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgc                                             <  1:4983113/101‑1 (MQ=255)
                                                        gcgcCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgc                                             <  1:3734904/101‑1 (MQ=255)
                                                         cgcCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgcg                                            <  1:622198/101‑1 (MQ=255)
                                                             gAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                                        >  1:4880069/1‑101 (MQ=255)
                                                             gAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                                        <  2:3833563/101‑1 (MQ=255)
                                                             gAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                                        <  2:1452236/101‑1 (MQ=255)
                                                             gAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                                        >  2:181340/1‑101 (MQ=255)
                                                               aaTTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCt                                      >  2:5268371/1‑101 (MQ=255)
                                                                aTTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGGCGAGGGGGCAGCGCCCCCtt                                     >  1:5593648/1‑101 (MQ=255)
                                                                aTTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtt                                     >  2:4433174/1‑101 (MQ=255)
                                                                  tCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                                   >  2:2707773/1‑101 (MQ=255)
                                                                  tCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                                   <  1:3154273/101‑1 (MQ=255)
                                                                  tCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                                   >  1:5688527/1‑101 (MQ=255)
                                                                   cACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTc                                  >  2:1280410/1‑101 (MQ=255)
                                                                   cACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTc                                  >  1:6455480/1‑101 (MQ=255)
                                                                    aCCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTcc                                 >  2:1844263/1‑101 (MQ=255)
                                                                    aCCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTcc                                 >  2:650343/1‑101 (MQ=255)
                                                                      cccgGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcg                               <  1:2153540/101‑1 (MQ=255)
                                                                        ccGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACTTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtc                             <  2:6950961/101‑1 (MQ=255)
                                                                          gggATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                           >  2:2934947/1‑101 (MQ=255)
                                                                            gATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCGCCCTTTTCCcggcgggg                         >  2:2268743/1‑98 (MQ=38)
                                                                            gATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtgg                         >  2:1040747/1‑100 (MQ=255)
                                                                             atatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGtgtg                        >  1:3823803/1‑101 (MQ=255)
                                                                             atatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGtgtg                        <  2:760988/101‑1 (MQ=255)
                                                                             atatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGtgtg                        >  2:3231133/1‑101 (MQ=255)
                                                                              tatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg                       >  2:4216908/1‑101 (MQ=255)
                                                                              tatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg                       <  2:6186756/101‑1 (MQ=255)
                                                                              tatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg                       <  1:1763221/101‑1 (MQ=255)
                                                                                tttGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGAt                     <  1:171893/101‑1 (MQ=255)
                                                                                 ttGAGGCAATATAAACGGGCGCCTATCGCCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                    <  2:866112/101‑1 (MQ=37)
                                                                                 ttGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                    >  1:3437289/1‑101 (MQ=255)
                                                                                 ttGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                    <  2:4781184/101‑1 (MQ=255)
                                                                                  tGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg                   >  1:1137151/1‑101 (MQ=255)
                                                                                  tGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg                   >  2:3954175/1‑101 (MQ=255)
                                                                                     ggCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCgcgc                >  2:668808/1‑101 (MQ=255)
                                                                                      gCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCa               >  2:3052072/1‑101 (MQ=255)
                                                                                      gCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCa               >  2:2562792/1‑101 (MQ=255)
                                                                                       cAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCaa              <  1:974382/101‑1 (MQ=255)
                                                                                       cAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCaa              <  2:2151890/101‑1 (MQ=255)
                                                                                       cAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCaa              >  2:2629670/1‑101 (MQ=255)
                                                                                       cAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCCCGCaa              >  2:378207/1‑101 (MQ=255)
                                                                                        aaGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAAc             >  2:4519103/1‑101 (MQ=255)
                                                                                         agataaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACg            <  1:6700663/96‑1 (MQ=255)
                                                                                         agataaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACg            >  1:2661117/6‑101 (MQ=255)
                                                                                          gataaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGt           <  1:3419831/97‑1 (MQ=255)
                                                                                           ataaccGGGCGCCTATCACCTTGGTGCAAATATCCGGGGCGTGATGGCGAAAGCCGAGGGGGCAGCTCCCTCTTTTCCCGTCGTGTGGATCGCGCAACGtt          <  2:5043648/96‑1 (MQ=255)
                                                                                           ataaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGtt          >  1:1574792/4‑101 (MQ=255)
                                                                                            taaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTc         <  1:331282/99‑1 (MQ=255)
                                                                                             aaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCa        <  1:4467587/100‑1 (MQ=255)
                                                                                               aCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAgg      >  2:6029132/1‑101 (MQ=255)
                                                                                               aCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAgg      <  1:4112905/101‑1 (MQ=255)
                                                                                               aCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAgg      >  2:2831152/1‑101 (MQ=255)
                                                                                                cGGGCGCCTATCGCCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATAGCGCAACGTTCAGGc     >  2:5198084/1‑101 (MQ=255)
                                                                                                 gggCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCg    >  2:2531015/1‑101 (MQ=255)
                                                                                                  ggCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCgg   >  1:4512862/1‑101 (MQ=255)
                                                                                                   gcgcCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGa  >  2:2104987/1‑101 (MQ=255)
                                                                                                   |                                                                                                    
GTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGAT.AACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGA  >  NC_014034/580862‑581060

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG