Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 580,966 A→T 1.4% intergenic (+87/‑284) RCAP_rcc00541 → / → RCAP_rcc00542 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034580,9660A→T 1.4% 4.2 ‑1.7 ‑0.0367intergenic (+87/‑284)RCAP_rcc00541/RCAP_rcc00542hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (194/167):  total (195/172)
Fisher's exact test strand distribution p-value = 1.89e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.25e-01

Only 100 of 400 total aligned reads displayed.

CCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.AACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCT  >  NC_014034/580867‑581066
                                                                                                     |                                                                                                     
 cGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAt                                                                                                      <  1:1722675/101‑1 (MQ=255)
  gCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.c                                                                                                    <  2:651121/101‑1 (MQ=255)
  gCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.c                                                                                                    <  1:3672500/101‑1 (MQ=255)
   ccTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.Ca                                                                                                   <  2:585674/101‑1 (MQ=255)
      cGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCt                                                                                                >  1:7057443/1‑101 (MQ=255)
      cGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCt                                                                                                <  1:4047102/101‑1 (MQ=255)
       gATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCtt                                                                                               >  1:1973630/1‑101 (MQ=255)
        cttGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTg                                                                                              <  1:5467036/100‑1 (MQ=255)
        aTTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTTGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAAAGGGCGCCTAT.CACCTTg                                                                                              >  2:150335/1‑101 (MQ=21)
         ttGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTgg                                                                                             >  2:5538234/1‑101 (MQ=255)
                ggcCCGGGCAGGGGGCGCTGCCCCCTCTTTTGGCCGCGCCGGAATTCA.CCCCCCGGGTATTTTAGGCAAGATAAACGGGCGCCGATCCACCTTGGGCCaaa                                                                                       >  2:2390877/1‑101 (MQ=12)
                  cccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAatat                                                                                    <  2:446895/101‑1 (MQ=255)
                   ccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATc                                                                                   <  2:3031470/101‑1 (MQ=255)
                       gCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCggg                                                                               >  2:3220216/1‑101 (MQ=255)
                         aGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCggggg                                                                             <  2:7001257/101‑1 (MQ=255)
                          gggggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCgggggg                                                                            >  2:4257454/1‑101 (MQ=255)
                           ggggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCggggggg                                                                           >  2:6021891/1‑100 (MQ=255)
                            gggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTg                                                                          <  1:5807641/101‑1 (MQ=255)
                            gggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTg                                                                          <  2:7088240/101‑1 (MQ=255)
                            gggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGGGCAAATATACGGGGGGTg                                                                          >  1:3833726/1‑101 (MQ=25)
                             ggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGa                                                                         <  1:152622/101‑1 (MQ=255)
                                  tGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCg                                                                    >  1:4175412/1‑101 (MQ=255)
                                   gCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGa                                                                   <  1:6053294/101‑1 (MQ=255)
                                    cccccTCTTTCGGCTGCGCCGAAATTCA.CCCCCCGGGTATTTGAGGCAAGATAAACGGGCGCCTAT.CATCTTGGTGCAAATATCCGGGGGGGGAGGCCGaa                                                                  >  2:5256342/1‑101 (MQ=37)
                                     ccccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CGCCTTGGTGCAAATATCCGGGGGGTGAGGCCGaaa                                                                 <  1:4506238/101‑1 (MQ=255)
                                     ccccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGaaa                                                                 <  1:6522080/101‑1 (MQ=255)
                                       ccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGc                                                               >  1:2715585/1‑101 (MQ=255)
                                        ctctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGcc                                                              <  1:4925462/101‑1 (MQ=255)
                                         tctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                                             >  1:725394/1‑101 (MQ=255)
                                          ctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGa                                                            >  2:5948744/1‑101 (MQ=255)
                                           tttCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAg                                                           <  2:4605267/101‑1 (MQ=255)
                                            ttCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.TACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgg                                                          <  2:3461487/101‑1 (MQ=255)
                                              cGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgggg                                                        >  1:5642229/1‑101 (MQ=255)
                                                gCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGc                                                      <  1:4437119/101‑1 (MQ=255)
                                                gCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGc                                                      <  1:3765362/101‑1 (MQ=255)
                                                 cTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCa                                                     <  2:6669094/101‑1 (MQ=255)
                                                 cTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCa                                                     >  2:2332169/1‑101 (MQ=255)
                                                  tGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAg                                                    >  2:1011600/1‑101 (MQ=255)
                                                  tGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAg                                                    <  1:3904287/101‑1 (MQ=255)
                                                    tcggccaaTTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGATGGGGTT.TATCTTGCCCCAAATATCCCGGGGGTGAGG.CG.AAGCCGAGGGGGCAgcgc                                                 <  2:6141742/95‑1 (MQ=37)
                                                    cgcCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgcg                                                  >  2:2697472/1‑101 (MQ=255)
                                                       cGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccc                                               <  2:2884903/101‑1 (MQ=255)
                                                        gAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                                              >  2:2171349/1‑101 (MQ=255)
                                                        gAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                                              <  1:571482/101‑1 (MQ=255)
                                                        gAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                                              >  1:5348592/1‑101 (MQ=255)
                                                         aaaTTCC.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc                                             <  1:350837/101‑1 (MQ=255)
                                                         aaaTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc                                             >  1:4747355/1‑101 (MQ=255)
                                                         aaaTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc                                             <  2:1973558/101‑1 (MQ=255)
                                                           aTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtt                                           >  2:6333016/1‑101 (MQ=255)
                                                           aTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtt                                           >  2:5351818/1‑101 (MQ=255)
                                                            ttCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCttt                                          >  2:1192852/1‑101 (MQ=255)
                                                             tCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                                         <  1:1656683/101‑1 (MQ=255)
                                                             tCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                                         <  2:2744305/101‑1 (MQ=255)
                                                             tCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                                         >  2:6700321/1‑101 (MQ=255)
                                                             tCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                                         <  1:3154273/101‑1 (MQ=255)
                                                              cA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTc                                        >  2:484115/1‑101 (MQ=255)
                                                              cA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTc                                        <  1:6231219/101‑1 (MQ=255)
                                                              cA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTc                                        >  1:6455480/1‑101 (MQ=255)
                                                               a.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTcc                                       >  2:1844263/1‑101 (MQ=255)
                                                                 cccccGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTccc                                      <  2:2569389/101‑1 (MQ=255)
                                                                  cccgGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcg                                     <  1:2153540/101‑1 (MQ=255)
                                                                    ccGGGATATTTGAGGCAAGATAAACGGGCGCCTAT.CACTTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtc                                   <  2:6950961/101‑1 (MQ=255)
                                                                      gggATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                                 >  2:3833162/1‑101 (MQ=255)
                                                                       ggATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtg                                >  1:1301125/1‑101 (MQ=255)
                                                                        gATATTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtgt                               <  1:6873609/101‑1 (MQ=255)
                                                                         atatTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGtgtg                              >  1:5576897/1‑101 (MQ=255)
                                                                         atatTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGtgtg                              <  2:760988/101‑1 (MQ=255)
                                                                          tatTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg                             <  2:5073985/101‑1 (MQ=255)
                                                                           atTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGTGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa                            >  1:4858302/1‑101 (MQ=255)
                                                                           atTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa                            >  2:3360638/1‑101 (MQ=255)
                                                                           atTTGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa                            >  2:3570718/1‑101 (MQ=255)
                                                                            tttGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGAt                           <  1:1841456/101‑1 (MQ=255)
                                                                             ttGAGGCAATATAAACGGGCGCCTAT.CGCCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                          <  2:866112/101‑1 (MQ=37)
                                                                             ttGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                          <  2:5641939/101‑1 (MQ=255)
                                                                             ttGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                          <  2:4175217/101‑1 (MQ=255)
                                                                              tGAGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg                         >  1:1137151/1‑101 (MQ=255)
                                                                                aGGCAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcgcg                       >  1:788099/1‑101 (MQ=255)
                                                                                   cAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCaa                    >  2:6445807/1‑101 (MQ=255)
                                                                                   cAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCaa                    >  2:1768587/1‑101 (MQ=255)
                                                                                   cAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCaa                    >  1:931488/1‑101 (MQ=255)
                                                                                   cAAGATAAACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCCCGCaa                    >  2:378207/1‑101 (MQ=255)
                                                                                     agataaaCGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACg                  <  1:6700663/96‑1 (MQ=255)
                                                                                      gataaaCGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGt                 <  1:3419831/97‑1 (MQ=255)
                                                                                        taaaCGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTc               <  1:331282/99‑1 (MQ=255)
                                                                                        taaaCGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTc               >  2:6593646/3‑101 (MQ=255)
                                                                                        taaaCGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTc               >  2:5145432/3‑101 (MQ=255)
                                                                                        taaaCGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTc               <  2:3905731/99‑1 (MQ=255)
                                                                                        taaaCGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGAGCGCGCAACGTTc               <  1:5140580/99‑1 (MQ=255)
                                                                                         aaaCGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCa              >  2:2115732/2‑101 (MQ=255)
                                                                                         aaaCGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCa              <  1:4550983/100‑1 (MQ=255)
                                                                                          aaCGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAg             >  1:5261185/1‑101 (MQ=255)
                                                                                          aaCGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAg             <  1:2390971/101‑1 (MQ=255)
                                                                                             gggCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCg          >  2:2531015/1‑101 (MQ=255)
                                                                                             gggCGCCTAT.CAACTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCg          >  2:1367999/1‑101 (MQ=255)
                                                                                               gcgcCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGa        >  1:5877550/1‑101 (MQ=255)
                                                                                                  ccTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGAttt     <  2:228530/101‑1 (MQ=255)
                                                                                                    tAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtc   <  1:2608601/101‑1 (MQ=255)
                                                                                                     aT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtct  <  1:695896/101‑1 (MQ=255)
                                                                                                     aT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtct  >  1:6204232/1‑101 (MQ=255)
                                                                                                     aT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtct  >  2:6836939/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
CCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.AACGGGCGCCTAT.CACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCT  >  NC_014034/580867‑581066

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG