Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 580,976 T→C 1.3% intergenic (+97/‑274) RCAP_rcc00541 → / → RCAP_rcc00542 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034580,9760T→C 1.3% 2.2 ‑1.6 ‑0.6382intergenic (+97/‑274)RCAP_rcc00541/RCAP_rcc00542hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (200/176):  total (202/180)
Fisher's exact test strand distribution p-value = 1.93e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.75e-01

Only 100 of 399 total aligned reads displayed.

TGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.AA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCA  >  NC_014034/580877‑581076
                                                                                                      |                                                                                                    
tGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGt                                                                                                      >  2:2468303/1‑101 (MQ=255)
 gAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGGAAGATAAA.CGGGCGCCTATCACCTTGGTg                                                                                                     >  2:6980977/1‑101 (MQ=255)
 gAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTg                                                                                                     <  1:3156221/101‑1 (MQ=255)
     cggcCCGGGCATGTGGCGCTGCCCCCTCTTTCGGCTGCTCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCaaa                                                                                                 <  1:3647470/101‑1 (MQ=25)
     cggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCaaa                                                                                                 <  1:6000963/101‑1 (MQ=255)
      ggcCCGGGCAGGGGGCGGTGCCCCCTCTTTCGGCTGCGCCGAAATTTA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGGGCCTATGACCTTTGTGCaaaa                                                                                                >  2:5606578/1‑100 (MQ=21)
      ggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAat                                                                                                <  1:6883253/101‑1 (MQ=255)
        cccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAatat                                                                                              <  2:446895/101‑1 (MQ=255)
         ccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATc                                                                                             <  1:239339/101‑1 (MQ=255)
            ggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCgg                                                                                          <  2:6859292/101‑1 (MQ=255)
            ggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCgg                                                                                          <  1:6086612/101‑1 (MQ=255)
              cAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCgggg                                                                                        >  2:5778704/1‑101 (MQ=255)
               aGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTTA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCggggg                                                                                       >  2:2562381/1‑101 (MQ=255)
                gggggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCgggggg                                                                                      >  2:4257454/1‑101 (MQ=255)
                 ggggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCggggggg                                                                                     >  2:6021891/1‑100 (MQ=255)
                     cgcTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAgg                                                                                 <  2:6687152/101‑1 (MQ=255)
                        tGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCg                                                                              >  1:4175412/1‑101 (MQ=255)
                         gCCGCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGa                                                                             <  1:6635294/101‑1 (MQ=255)
                          cccccTCTTTCGGCTGCGCCGAAATTCA.CCCCCCGGGTATTTGAGGCAAGATAAA.CGGGCGCCTATCATCTTGGTGCAAATATCCGGGGGGGGAGGCCGaa                                                                            >  2:5256342/1‑101 (MQ=37)
                           ccccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCGCCTTGGTGCAAATATCCGGGGGGTGAGGCCGaaa                                                                           <  1:4506238/101‑1 (MQ=255)
                            cccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAg                                                                          >  1:737834/1‑101 (MQ=255)
                            cccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAg                                                                          <  1:4845328/101‑1 (MQ=255)
                             ccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGc                                                                         >  1:2715585/1‑101 (MQ=255)
                              ctctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGcc                                                                        <  1:4925462/101‑1 (MQ=255)
                               tctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                                                       <  2:6617835/101‑1 (MQ=255)
                                 tttCGGCTGCGCCGAAATTCA.CCCCCGGTATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAg                                                                     <  1:1373344/101‑1 (MQ=255)
                                  ttCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATTACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgg                                                                    <  2:3461487/101‑1 (MQ=255)
                                    cGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgggg                                                                  >  2:231965/1‑101 (MQ=255)
                                    cGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgggg                                                                  >  1:5642229/1‑101 (MQ=255)
                                    cGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgggg                                                                  >  1:1232238/1‑101 (MQ=255)
                                      gCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGc                                                                >  1:7018627/1‑101 (MQ=255)
                                      gCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGc                                                                <  1:4437119/101‑1 (MQ=255)
                                          tcggccaaTTCACCCCCCGGGATATTTGCGGCAAGGTGAA.AGGATGGGGTTTATCTTGCCCCAAATATCCCGGGGGTGAGG.CG.AAGCCGAGGGGGCAgcgc                                                           <  2:6141742/95‑1 (MQ=37)
                                          cgcCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgcg                                                            <  1:622198/101‑1 (MQ=255)
                                           gcCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgcgc                                                           >  2:4112711/1‑101 (MQ=255)
                                              gAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                                                        <  1:571482/101‑1 (MQ=255)
                                              gAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                                                        <  2:1452236/101‑1 (MQ=255)
                                               aaaTTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc                                                       >  1:4747355/1‑101 (MQ=255)
                                               aaaTTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc                                                       >  2:2365363/1‑101 (MQ=255)
                                               aaaTTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc                                                       >  1:2130001/1‑101 (MQ=255)
                                                aaTTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCt                                                      <  2:776984/101‑1 (MQ=255)
                                                 aTTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtt                                                     <  2:2628750/101‑1 (MQ=255)
                                                 aTTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtt                                                     >  2:5351818/1‑101 (MQ=255)
                                                 aTTCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtt                                                     <  2:2130905/101‑1 (MQ=255)
                                                  ttCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCttt                                                    >  2:1192852/1‑101 (MQ=255)
                                                  ttCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCttt                                                    >  2:1759325/1‑101 (MQ=255)
                                                   tCA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                                                   >  2:2707773/1‑101 (MQ=255)
                                                    cA.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTc                                                  >  2:484115/1‑101 (MQ=255)
                                                     a.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTcc                                                 >  2:1844263/1‑101 (MQ=255)
                                                     a.CCCCCGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTcc                                                 >  2:650343/1‑101 (MQ=255)
                                                      ccccccGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTcc                                                 <  2:6412295/100‑1 (MQ=255)
                                                          ccGGGATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtc                                             <  1:3777183/101‑1 (MQ=255)
                                                            gggATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                                           >  2:3569573/1‑101 (MQ=255)
                                                            gggATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                                           >  2:2934947/1‑101 (MQ=255)
                                                            gggATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                                           >  2:3833162/1‑101 (MQ=255)
                                                             ggATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtg                                          >  1:6477535/1‑101 (MQ=255)
                                                             ggATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtg                                          >  2:2093089/1‑101 (MQ=255)
                                                              gagatTTGAGGCAAGAA.AATCGGGCGCCTATCCCCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtgt                                         <  2:7074270/98‑1 (MQ=37)
                                                              gATATTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCGCCCTTTTCCcggcgggg                                         >  2:2268743/1‑98 (MQ=38)
                                                               atatTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGtgtg                                        >  1:5576897/1‑101 (MQ=255)
                                                                tatTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg                                       <  2:5073985/101‑1 (MQ=255)
                                                                tatTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg                                       >  1:1906260/1‑101 (MQ=255)
                                                                 atTTGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGTGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa                                      >  1:3412900/1‑101 (MQ=255)
                                                                  tttGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGAt                                     >  1:1532574/1‑101 (MQ=255)
                                                                   ttGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                                    >  1:3437289/1‑101 (MQ=255)
                                                                   ttGAGGCAAGATAAA.CGGGCGCCTATCACCTTCGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                                    <  2:3899334/101‑1 (MQ=255)
                                                                    tGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg                                   >  2:3778951/1‑101 (MQ=255)
                                                                    tGAGGCAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg                                   >  1:1137151/1‑101 (MQ=255)
                                                                         cAAGATAAA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCaa                              >  1:931488/1‑101 (MQ=255)
                                                                             ataaa.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGtt                          >  1:3839221/4‑101 (MQ=255)
                                                                              taaa.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTc                         <  1:331282/99‑1 (MQ=255)
                                                                              taaa.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTc                         <  2:31874/99‑1 (MQ=255)
                                                                              taaa.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGAGCGCGCAACGTTc                         <  1:5140580/99‑1 (MQ=255)
                                                                               aaa.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCa                        <  1:4550983/100‑1 (MQ=255)
                                                                                aa.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAg                       >  1:3975496/1‑101 (MQ=255)
                                                                                aa.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAg                       >  1:5261185/1‑101 (MQ=255)
                                                                                 agcggggcccTATCCCCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGGGTGGATCGCGCAACGTTCAg                       <  2:3313793/93‑1 (MQ=37)
                                                                                 a.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAgg                      >  2:2831152/1‑101 (MQ=255)
                                                                                 a.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAgg                      <  2:725349/101‑1 (MQ=255)
                                                                                   cGGGCGCCTATCGCCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGc                     <  1:6732818/101‑1 (MQ=255)
                                                                                      gcgcCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGa                  >  1:5877550/1‑101 (MQ=255)
                                                                                       cgcCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGAt                 <  2:4540305/101‑1 (MQ=255)
                                                                                          cTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGAtttt              <  1:5359193/101‑1 (MQ=255)
                                                                                          cTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTGCAGGCGGAtttt              <  1:6333341/101‑1 (MQ=255)
                                                                                          cTATCACCTTGGTGCAAATACCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGAtgtt              >  2:6803233/1‑101 (MQ=255)
                                                                                           tATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtc             <  1:2608601/101‑1 (MQ=255)
                                                                                           tATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtc             >  1:1779160/1‑101 (MQ=255)
                                                                                           tATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtc             >  2:5932973/1‑101 (MQ=255)
                                                                                            ttCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtct            <  1:816514/100‑1 (MQ=255)
                                                                                            aTCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtct            >  2:6320144/1‑101 (MQ=255)
                                                                                              ttccTTGGTGCAAAAGGACTGGGTGTGAGTCCGAAAGCCGAGGGGGCAGCGCGCCCTTTTCCCTTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTcc          <  2:880737/99‑1 (MQ=255)
                                                                                              cACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTcc          >  2:1940815/1‑101 (MQ=255)
                                                                                                ccTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCt        <  2:5152816/101‑1 (MQ=255)
                                                                                                ccTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCt        <  1:5790396/101‑1 (MQ=255)
                                                                                                 cTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTg       >  2:1344388/1‑101 (MQ=255)
                                                                                                 cTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTg       <  1:5481692/101‑1 (MQ=255)
                                                                                                  ttGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGc      <  2:338044/101‑1 (MQ=255)
                                                                                                   tGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCg     >  2:1034141/1‑101 (MQ=255)
                                                                                                     gTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGc   >  1:1658738/1‑101 (MQ=255)
                                                                                                      tGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCa  <  1:2171429/101‑1 (MQ=255)
                                                                                                      |                                                                                                    
TGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.AA.CGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCA  >  NC_014034/580877‑581076

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG