Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 580,987 G→C 1.3% intergenic (+108/‑263) RCAP_rcc00541 → / → RCAP_rcc00542 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034580,9870G→C 1.3% 2.2 ‑1.6 ‑0.7386intergenic (+108/‑263)RCAP_rcc00541/RCAP_rcc00542hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (203/178):  total (204/182)
Fisher's exact test strand distribution p-value = 1.93e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.88e-01

Only 100 of 422 total aligned reads displayed.

GGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.AACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCT  >  NC_014034/580888‑581087
                                                                                                     |                                                                                                    
 ggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCgg                                                                                                     >  2:3210142/1‑101 (MQ=255)
   cAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCgggg                                                                                                   >  2:5580496/1‑101 (MQ=255)
     gggggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCgggggg                                                                                                 >  1:4822284/1‑101 (MQ=255)
       gggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGGGCAAATATACGGGGGGTg                                                                                               >  1:3833726/1‑101 (MQ=25)
         gcgcTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAg                                                                                             <  1:1535487/101‑1 (MQ=255)
         gcgcTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAg                                                                                             <  1:2161608/101‑1 (MQ=255)
             tGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCg                                                                                         >  1:4175412/1‑101 (MQ=255)
             tGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCg                                                                                         >  2:6698932/1‑101 (MQ=255)
                ccccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCGCCTTGGTGCAAATATCCGGGGGGTGAGGCCGaaa                                                                                      <  1:4506238/101‑1 (MQ=255)
                ccccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGaaa                                                                                      >  2:350093/1‑101 (MQ=255)
                 cccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAg                                                                                     >  2:533432/1‑101 (MQ=255)
                    tctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                                                                  >  1:725394/1‑101 (MQ=255)
                    tctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                                                                  <  2:3722473/101‑1 (MQ=255)
                     ctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGa                                                                                 >  2:5948744/1‑101 (MQ=255)
                       ttCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgg                                                                               >  2:6659112/1‑101 (MQ=255)
                         cGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgggg                                                                             >  2:231965/1‑101 (MQ=255)
                           gCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGc                                                                           >  1:7018627/1‑101 (MQ=255)
                           gCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGc                                                                           <  1:4437119/101‑1 (MQ=255)
                           gCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGCGGTGAGGCCGAAAGCCGAGGGGGc                                                                           <  2:3404812/101‑1 (MQ=255)
                             tGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAg                                                                         >  2:1011600/1‑101 (MQ=255)
                             cttcggccaaTTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGATGGGGTTTATCTTGCCGCAAATATCCCGGGGGTGAGG.CG.AAGCCGAGGGGGCAgc                                                                        >  1:5097686/9‑101 (MQ=25)
                              gcgcCGAAATTCC.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCCCCTTGGTGCAAATACCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgc                                                                        <  2:6060511/101‑1 (MQ=38)
                               tcggccaaTTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGATGGGGTTTATCTTGCCCCAAATATCCCGGGGGTGAGG.CG.AAGCCGAGGGGGCAgcgc                                                                      <  2:7075430/95‑1 (MQ=37)
                                  tgAAATTCA.CCTCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccc                                                                    <  2:3332408/100‑1 (MQ=255)
                                  cGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccc                                                                    <  2:2884903/101‑1 (MQ=255)
                                    aaaTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc                                                                  >  2:2365363/1‑101 (MQ=255)
                                    aaaTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc                                                                  <  1:4907135/101‑1 (MQ=255)
                                    aaaTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc                                                                  >  1:4747355/1‑101 (MQ=255)
                                     aaTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCt                                                                 <  2:776984/101‑1 (MQ=255)
                                      aTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGGCGAGGGGGCAGCGCCCCCtt                                                                >  1:5593648/1‑101 (MQ=255)
                                      aTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtt                                                                <  2:2628750/101‑1 (MQ=255)
                                      aTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtt                                                                >  2:4433174/1‑101 (MQ=255)
                                      aTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtt                                                                >  2:6333016/1‑101 (MQ=255)
                                      aTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtt                                                                <  2:2130905/101‑1 (MQ=255)
                                       ttCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCttt                                                               >  2:1759325/1‑101 (MQ=255)
                                        tCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                                                              >  1:5718761/1‑101 (MQ=255)
                                        tCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                                                              >  2:2707773/1‑101 (MQ=255)
                                          a.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTcc                                                            >  2:1844263/1‑101 (MQ=255)
                                               ccGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtc                                                        <  1:3777183/101‑1 (MQ=255)
                                                cGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcg                                                       >  2:1913376/1‑101 (MQ=255)
                                                 gggATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                                                      >  1:5841033/1‑101 (MQ=255)
                                                 gggATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                                                      >  2:3833162/1‑101 (MQ=255)
                                                  ggATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtg                                                     >  1:6477535/1‑101 (MQ=255)
                                                  ggATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtg                                                     >  1:1301125/1‑101 (MQ=255)
                                                  ggATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtg                                                     >  2:2093089/1‑101 (MQ=255)
                                                   gATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCGCCCTTTTCCcggcgggg                                                    >  2:2268743/1‑98 (MQ=38)
                                                   gATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtgt                                                    <  1:6873609/101‑1 (MQ=255)
                                                   gATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtgt                                                    <  1:5655291/101‑1 (MQ=255)
                                                    atatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGCGAGAGGCCGACAACCGAGGGGGCAGCTCCCCCCTTTTCcgctgtttg                                                   >  2:68123/1‑94 (MQ=255)
                                                     tatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg                                                  >  2:4216908/1‑101 (MQ=255)
                                                     tatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg                                                  <  2:5073985/101‑1 (MQ=255)
                                                      atTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGTGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa                                                 >  1:3412900/1‑101 (MQ=255)
                                                      atTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa                                                 >  2:149602/1‑101 (MQ=255)
                                                        ttGAGGCAATATAAACGGGCGCCTATCGCCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                                               <  2:866112/101‑1 (MQ=37)
                                                        ttGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                                               <  2:4781184/101‑1 (MQ=255)
                                                        ttGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                                               >  1:3437289/1‑101 (MQ=255)
                                                         tGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg                                              >  1:1137151/1‑101 (MQ=255)
                                                          gAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcgc                                             >  2:2968722/1‑101 (MQ=255)
                                                           aGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcgcg                                            >  1:788099/1‑101 (MQ=255)
                                                             gCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCa                                          >  2:2562792/1‑101 (MQ=255)
                                                              cAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCaa                                         >  2:6445807/1‑101 (MQ=255)
                                                              cAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCaa                                         >  1:3251101/1‑101 (MQ=255)
                                                               aaGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAAc                                        <  1:2707875/101‑1 (MQ=255)
                                                               aaGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAAc                                        >  2:4519103/1‑101 (MQ=255)
                                                                   taaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTc                                    <  2:3905731/99‑1 (MQ=255)
                                                                     aaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAg                                  >  1:3975496/1‑101 (MQ=255)
                                                                     aaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAg                                  >  1:5261185/1‑101 (MQ=255)
                                                                      aCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAgg                                 >  2:584735/1‑101 (MQ=255)
                                                                       cGGGCGCCTATCGCCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATAGCGCAACGTTCAGGc                                >  2:5198084/1‑101 (MQ=255)
                                                                       cGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTATTCCCGTCGTGTGGATCGCGCAACGTTCAGGc                                <  2:725374/101‑1 (MQ=255)
                                                                        gggCGCCTATCAACTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCg                               >  2:1367999/1‑101 (MQ=255)
                                                                             ccTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGAttt                          <  1:1780864/101‑1 (MQ=255)
                                                                              cTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGAtttt                         <  1:5359193/101‑1 (MQ=255)
                                                                              cTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGAtttt                         <  1:1911999/101‑1 (MQ=255)
                                                                              cTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTGCAGGCGGAtttt                         <  1:6333341/101‑1 (MQ=255)
                                                                                aTCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtct                       >  2:1121748/1‑101 (MQ=255)
                                                                                aTCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtct                       >  1:910113/1‑101 (MQ=255)
                                                                                 tCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTtctc                      >  2:6143463/1‑101 (MQ=255)
                                                                                    ccTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCt                   <  1:6716655/101‑1 (MQ=255)
                                                                                     cTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTg                  <  1:5481692/101‑1 (MQ=255)
                                                                                     cTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTg                  >  2:2507830/1‑101 (MQ=255)
                                                                                        ggTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCgg               <  2:7000036/101‑1 (MQ=255)
                                                                                         gTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGc              >  1:1658738/1‑101 (MQ=255)
                                                                                           gCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCAt            <  2:6571336/101‑1 (MQ=255)
                                                                                            cAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATc           <  1:5784172/101‑1 (MQ=255)
                                                                                            cAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATc           <  1:6221944/101‑1 (MQ=255)
                                                                                               atatCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCt        >  2:4981290/1‑101 (MQ=255)
                                                                                               atatCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCt        >  2:6877081/1‑101 (MQ=255)
                                                                                                tatCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTg       <  1:3076606/101‑1 (MQ=255)
                                                                                                tatCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTg       <  2:1232190/101‑1 (MQ=255)
                                                                                                 atCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGc      >  1:2128162/1‑101 (MQ=255)
                                                                                                 atCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGc      <  2:2771398/101‑1 (MQ=255)
                                                                                                 atCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGc      >  1:1435152/1‑101 (MQ=255)
                                                                                                   ccGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCAt    <  1:3570854/101‑1 (MQ=255)
                                                                                                   ccGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCAt    <  1:2562475/101‑1 (MQ=255)
                                                                                                   ccGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCAt    <  1:6088985/101‑1 (MQ=255)
                                                                                                    cGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATc   <  1:2383416/101‑1 (MQ=255)
                                                                                                    cGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATc   >  1:5976560/1‑101 (MQ=255)
                                                                                                     ggggggTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCt  >  1:455101/1‑101 (MQ=255)
                                                                                                     ggggggTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCt  >  2:4087911/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
GGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.AACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGGATTTTCTCCCTGCGGCATCGCTGCATCT  >  NC_014034/580888‑581087

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG