Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 590,814 C→G 100% Q56E (CAA→GAA)  murI → glutamate racemase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034590,8140C→G100.0% 1191.0 321Q56E (CAA→GAA) murIglutamate racemase
Reads supporting (aligned to +/- strand):  new base (143/177):  ref base (0/1):  total (143/178)

Only 100 of 337 total aligned reads displayed.

TGCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCCAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGAA  >  NC_014034/590716‑590911
                                                                                                  |                                                                                                 
tGCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGaa                                                                                                 <  1:4674320/101‑1 (MQ=255)
tGCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGaa                                                                                                 <  2:3584427/101‑1 (MQ=255)
 tcccGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAAc                                                                                                <  1:248419/100‑1 (MQ=255)
 gCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAAc                                                                                                >  2:6137381/1‑101 (MQ=255)
    cGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGAGCTGCCGCGGCGTCGAACgga                                                                                             >  1:5617577/1‑100 (MQ=255)
     gAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGcg                                                                                            >  2:3656338/1‑100 (MQ=255)
       ggTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCTGCGTCGAACGGCtgt                                                                                          <  2:5467516/101‑1 (MQ=255)
       ggTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCtgt                                                                                          >  2:1879636/1‑101 (MQ=255)
        gTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCtgtg                                                                                         >  1:3924713/1‑101 (MQ=255)
        gTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCtgtg                                                                                         <  2:1371908/101‑1 (MQ=255)
         tGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTgg                                                                                        >  2:464972/1‑101 (MQ=255)
          ggCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTggg                                                                                       >  1:1015892/1‑101 (MQ=255)
          ggCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTggg                                                                                       >  2:3002651/1‑101 (MQ=255)
             cTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACg                                                                                    <  2:2895814/101‑1 (MQ=255)
             cTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCAACCCCGACGACATCTACAACCTGACATACCCCGGGGGCGAACGGCGGGGGGGCg                                                                                    >  1:2277347/1‑101 (MQ=255)
               ttGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTTGGACGAg                                                                                  >  2:6688331/1‑101 (MQ=255)
               cgatCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAg                                                                                  <  1:3738079/98‑1 (MQ=255)
                tGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAgg                                                                                 <  1:4444266/101‑1 (MQ=255)
                  tCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGc                                                                               >  2:731075/1‑101 (MQ=255)
                   cTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCt                                                                              <  2:5541644/101‑1 (MQ=255)
                     attatGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGGGTCGAACGGCTGTGGGACGCGGGCTGc                                                                            >  2:4492749/1‑101 (MQ=255)
                     attatGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAgagatcg                                                                            >  2:3737929/1‑95 (MQ=255)
                      ttatGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCTGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCg                                                                           <  1:5003637/101‑1 (MQ=255)
                        atGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGAt                                                                         >  1:5086260/1‑101 (MQ=255)
                          ggCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCt                                                                       <  1:93386/101‑1 (MQ=255)
                          ggCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCt                                                                       >  1:3999056/1‑101 (MQ=255)
                           gccaCAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTg                                                                      <  1:4183809/98‑1 (MQ=255)
                           gCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTg                                                                      >  2:7013249/1‑101 (MQ=255)
                            cGACAATGCCCATGCGCCCTACGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTgg                                                                     <  1:1099010/101‑1 (MQ=255)
                            cGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTgg                                                                     <  1:3791032/101‑1 (MQ=255)
                               cAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGa                                                                  >  2:1935156/1‑101 (MQ=255)
                               cAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGa                                                                  >  2:3969354/1‑101 (MQ=255)
                                aaTGCCCATGCGCCCTACGGCGTGCGCACCCCGGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTTGTGAt                                                                 >  1:5426580/1‑101 (MQ=255)
                                aaTGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGAt                                                                 >  1:4264273/1‑101 (MQ=255)
                                aaTGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGAt                                                                 <  2:2401646/101‑1 (MQ=255)
                                 aTGCCCATGCGCCCTACGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGAtt                                                                <  2:3150865/101‑1 (MQ=255)
                                  ggCCCATGCGCCCGTCGGCGTGCCCACCCCCGCCGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTc                                                               <  2:1998790/100‑1 (MQ=255)
                                   gCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCt                                                              <  1:2016005/101‑1 (MQ=255)
                                   gCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCt                                                              <  2:6491257/101‑1 (MQ=255)
                                     ccATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGGACGGCTGGGGGACGAGGGCTGGGACCTGGGGGTTCTgg                                                            >  1:2800896/1‑101 (MQ=255)
                                     ccATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTgg                                                            >  2:1724771/1‑101 (MQ=255)
                                      cATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGCCGAGGGCTGCGCTCTGGTGATTCTGGc                                                           >  1:149683/1‑101 (MQ=255)
                                       aTGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGcc                                                          >  1:6347692/1‑101 (MQ=255)
                                       aTGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGcc                                                          >  1:3432403/1‑101 (MQ=255)
                                        tGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCt                                                         <  2:2206381/101‑1 (MQ=255)
                                         gcgcCCTACGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTg                                                        <  2:4055844/101‑1 (MQ=255)
                                         gcgcCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTg                                                        >  2:499272/1‑101 (MQ=255)
                                         gcgcCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTg                                                        <  1:5837560/101‑1 (MQ=255)
                                         gcgcCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTg                                                        >  2:320684/1‑101 (MQ=255)
                                           gcCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCa                                                      >  1:5467600/1‑101 (MQ=255)
                                             ccTACGGCGTTGGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGACTCTGGCCTGCAac                                                    <  2:1951793/101‑1 (MQ=255)
                                             ccTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAac                                                    >  1:6565574/1‑101 (MQ=255)
                                             ccTACGGCGGGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAac                                                    <  2:4428429/101‑1 (MQ=255)
                                              cTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAaca                                                   >  1:84630/1‑101 (MQ=255)
                                                aCGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACg                                                 >  2:4274684/1‑101 (MQ=255)
                                                 cGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACgg                                                <  2:4179110/101‑1 (MQ=255)
                                                  ggCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGc                                               >  1:1396340/1‑101 (MQ=255)
                                                   gCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGcc                                              <  2:5453931/101‑1 (MQ=255)
                                                     gTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTc                                            >  2:4736735/1‑101 (MQ=255)
                                                      tGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTcc                                           <  1:6137700/101‑1 (MQ=255)
                                                      tGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTcc                                           >  1:1249115/1‑101 (MQ=255)
                                                      tGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTcc                                           <  1:4224989/101‑1 (MQ=255)
                                                        cgcACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTccgc                                         <  2:1326253/101‑1 (MQ=255)
                                                         gcACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTccgcc                                        >  2:5159293/1‑101 (MQ=255)
                                                          cACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTccgccg                                       >  1:312802/1‑101 (MQ=255)
                                                          cACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTccgccg                                       >  1:3569751/1‑101 (MQ=255)
                                                           ccccccGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCcgccgc                                      <  1:320688/100‑1 (MQ=255)
                                                             ccccGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCgcgg                                    >  2:1246048/1‑101 (MQ=255)
                                                             ccccGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCgcgg                                    >  1:4388228/1‑101 (MQ=255)
                                                             ccccGACGACATCTACAACCTGACCTGCCGCGGCGTCCAACGGCTGTGGGACGAGGGCTGCGATCCGGTGATTCTGGCCTGCAACACGGCCTCCGCCgcgg                                    <  1:6411364/101‑1 (MQ=255)
                                                              cccGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCgcggc                                   >  2:2083976/1‑101 (MQ=255)
                                                                 gacgacATCTACACCCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCt                                <  1:6003988/101‑1 (MQ=255)
                                                                 gacgacATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCt                                >  2:1504503/1‑101 (MQ=255)
                                                                    ggccgcgaCAACCGGCCCTGCCGCGGCGTCGAGCGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGaa                             <  1:2184827/94‑1 (MQ=255)
                                                                     acATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGaaa                            <  1:5799790/101‑1 (MQ=255)
                                                                       aTCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAg                          <  1:4820246/101‑1 (MQ=255)
                                                                        tcgaCAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAgg                         <  2:5860896/98‑1 (MQ=255)
                                                                          tACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGAt                       >  2:6803633/1‑101 (MQ=255)
                                                                          tACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGAt                       >  1:6734831/1‑101 (MQ=255)
                                                                           aCAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATg                      >  1:6875725/1‑101 (MQ=255)
                                                                                ctgccctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGa                 <  1:1548028/101‑1 (MQ=255)
                                                                                 tgacctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGaa                >  1:2395102/1‑101 (MQ=255)
                                                                                  gccctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAAc               <  2:5365820/99‑1 (MQ=255)
                                                                                  gacctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAAc               <  2:3791926/101‑1 (MQ=255)
                                                                                   acctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACa              <  2:5929082/101‑1 (MQ=255)
                                                                                    cctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGTTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACAt             <  1:6438184/101‑1 (MQ=255)
                                                                                    cctgCCGCGGCCTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACAt             <  2:3295572/101‑1 (MQ=255)
                                                                                     ctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACAtt            <  2:1015857/101‑1 (MQ=255)
                                                                                     ctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACAtt            <  2:5794057/101‑1 (MQ=255)
                                                                                     ctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACAtt            <  2:2487433/101‑1 (MQ=255)
                                                                                      tgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTg           >  2:6536011/1‑101 (MQ=255)
                                                                                         cgcgGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGAt        >  2:2800170/1‑101 (MQ=255)
                                                                                          gcggcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATc       <  1:5342434/101‑1 (MQ=255)
                                                                                          gcggcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATc       >  2:3482819/1‑101 (MQ=255)
                                                                                           gggcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATcc      <  2:6627036/100‑1 (MQ=255)
                                                                                           cggcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATcc      <  2:6392201/101‑1 (MQ=255)
                                                                                           cggcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATcc      <  2:6212367/101‑1 (MQ=255)
                                                                                            ggcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATccc     <  1:2880599/101‑1 (MQ=255)
                                                                                            ggcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATccc     >  1:3637782/1‑101 (MQ=255)
                                                                                             gcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCg    >  1:1970531/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
TGCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCCAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGAA  >  NC_014034/590716‑590911

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG