Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 678,779 C→T 9.7% A1610T (GCG→ACG)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034678,7790C→T 9.7% 81.4 ‑1.0 ‑0.2341A1610T (GCG→ACG) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (17/16):  ref base (185/123):  total (202/139)
Fisher's exact test strand distribution p-value = 3.57e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.61e-01

Only 100 of 357 total aligned reads displayed.

TGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTG  >  NC_014034/678679‑678879
                                                                                                    |                                                                                                     
      gTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCt                                                                                                 >  2:6880308/1‑101 (MQ=255)
       tGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTc                                                                                                <  2:5449390/101‑1 (MQ=255)
        gCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCa                                                                                               >  1:1108109/1‑101 (MQ=255)
        gCTCAGGCTTGGCATCAGCCCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCa                                                                                               <  2:282976/101‑1 (MQ=255)
         cTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAg                                                                                              >  1:1782167/1‑101 (MQ=255)
           cAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAgcg                                                                                            <  2:2528426/101‑1 (MQ=255)
             ggCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGcgcc                                                                                          >  1:7061362/1‑101 (MQ=255)
               cTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccgc                                                                                        >  1:4418196/1‑101 (MQ=255)
                 tGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGa                                                                                      >  1:379843/1‑101 (MQ=255)
                  ggCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGAt                                                                                     >  1:1692408/1‑101 (MQ=255)
                  ggCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGAt                                                                                     >  2:3368014/1‑101 (MQ=255)
                  ggCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGAt                                                                                     <  1:4134048/101‑1 (MQ=255)
                   gCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATc                                                                                    >  2:4624580/1‑101 (MQ=255)
                    cATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCt                                                                                   <  1:375319/101‑1 (MQ=255)
                     aTCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTg                                                                                  >  1:3574303/1‑101 (MQ=255)
                     aTCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCGGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTg                                                                                  >  2:3445148/1‑101 (MQ=255)
                         gcgcCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTc                                                                              <  2:4825173/101‑1 (MQ=255)
                         gcgcCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTc                                                                              <  1:2102286/101‑1 (MQ=255)
                             cGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCgcgc                                                                          >  2:2435773/1‑101 (MQ=255)
                               tCCCGTTCCCCGCCATGGCCGAGGCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTc                                                                        <  2:6949633/101‑1 (MQ=255)
                               tCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTc                                                                        >  1:1299533/1‑101 (MQ=255)
                               tCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTc                                                                        >  1:4926323/1‑101 (MQ=255)
                                 ccGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAg                                                                      >  1:1920631/1‑101 (MQ=255)
                                  cGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGc                                                                     >  2:6688112/1‑101 (MQ=255)
                                   gATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGcc                                                                    >  1:299478/1‑101 (MQ=255)
                                   gATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGcc                                                                    >  2:1995760/1‑101 (MQ=255)
                                   gATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGcc                                                                    <  2:7053469/101‑1 (MQ=255)
                                    aTCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCg                                                                   >  1:2681864/1‑101 (MQ=255)
                                           ccATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgccgcc                                                            <  1:2361103/101‑1 (MQ=255)
                                            cATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCt                                                           >  1:2162106/1‑101 (MQ=255)
                                             aTGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTg                                                          >  2:6807986/1‑101 (MQ=255)
                                             aTGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTg                                                          <  2:2110025/101‑1 (MQ=255)
                                              tGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTgc                                                         >  1:5857916/1‑101 (MQ=255)
                                              tGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTAGCGCTCAGCCGTGCCGCCTgc                                                         <  1:4212830/101‑1 (MQ=255)
                                               ggCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTgc.g                                                       <  2:270350/101‑1 (MQ=255)
                                                gCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTgc.gc                                                      >  1:4527642/1‑101 (MQ=255)
                                                gCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTgc.gc                                                      >  1:6549218/1‑101 (MQ=255)
                                                 ccGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.Gcc                                                     <  2:3534853/101‑1 (MQ=255)
                                                 ccGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.Gcc                                                     <  2:4450993/101‑1 (MQ=255)
                                                   gagaCCGAAAGCTGGGCGATATCCGCGGGTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGAGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGt                                                   >  1:5070382/1‑101 (MQ=255)
                                                    agaCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTg                                                  <  1:5145053/101‑1 (MQ=255)
                                                        cGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCa                                              >  2:4993556/1‑101 (MQ=255)
                                                         gAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGGGCCGCGATCGGCGCAGCGCTCAGCCGTGCCGCCTGC.GCGGTggcgtg                                             >  2:3284325/1‑97 (MQ=37)
                                                         gAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAg                                             >  1:5372096/1‑101 (MQ=255)
                                                          aaaGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAgc                                            <  1:635442/101‑1 (MQ=255)
                                                          aaaGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAgc                                            >  1:909346/1‑101 (MQ=255)
                                                          aaaGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATATGCGTCGCGATCAGCCGGGCAGCCTGC.GCCCTGGGCAgc                                            >  1:6941192/1‑101 (MQ=37)
                                                           aaGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAgcg                                           >  1:3946202/1‑101 (MQ=255)
                                                             gCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGcc                                         >  2:6775805/1‑101 (MQ=255)
                                                              cTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCa                                        >  1:2764974/1‑101 (MQ=255)
                                                               tGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCAc                                       <  1:3056586/101‑1 (MQ=255)
                                                               tGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCAc                                       >  2:3154135/1‑101 (MQ=255)
                                                                gggCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACg                                      <  1:6240682/101‑1 (MQ=255)
                                                                 caccagatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGC.GCCGTCGTCAGCGCCACGa                                     >  1:1469312/7‑101 (MQ=2)
                                                                   cGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATg                                   >  2:193660/1‑101 (MQ=255)
                                                                    gATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGt                                  >  1:4641203/1‑101 (MQ=255)
                                                                     atatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTc                                 >  2:3083550/1‑101 (MQ=255)
                                                                     atatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTc                                 >  2:6037160/1‑101 (MQ=255)
                                                                     atatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTc                                 <  2:1662735/101‑1 (MQ=255)
                                                                     atatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTc                                 >  2:923265/1‑101 (MQ=255)
                                                                     agatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGC.GCCGTCGTCAGCGCCACGATGTc                                 >  2:3904512/3‑101 (MQ=2)
                                                                     agatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGC.GCCGTCGTCAGCGCCACGATGTc                                 <  1:5694129/99‑1 (MQ=2)
                                                                     agatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGACGCCTGC.GCCGTCGTCAGCGCCACGATGTc                                 <  1:1054076/99‑1 (MQ=2)
                                                                      tatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCa                                <  1:6039160/101‑1 (MQ=255)
                                                                      gatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGC.GCCGTCGTCAGCGCCACGATGTCa                                <  1:5465918/100‑1 (MQ=2)
                                                                      gatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGC.GCCGTCGTCAGCGCCACGATGTCa                                >  1:2516627/2‑101 (MQ=2)
                                                                       atCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGC.GCCGTCGTCAGCGCCACGATGTCAt                               <  2:5953572/101‑1 (MQ=2)
                                                                       atCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCAt                               <  2:5106034/101‑1 (MQ=255)
                                                                       atCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCAt                               >  2:6368013/1‑101 (MQ=255)
                                                                        tcggcgGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGC.GCCGTCGTCAGCGCCACGATGTCAtc                              <  1:957196/98‑2 (MQ=9)
                                                                        tCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCAtt                              <  2:4362564/101‑1 (MQ=255)
                                                                         cggcgGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGC.GCCGTCGTCAGCGCCACGATGTCAtcg                             <  1:5246580/99‑3 (MQ=9)
                                                                          cgcgGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCg                            <  1:3414584/101‑1 (MQ=255)
                                                                           gcgGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGt                           >  2:1374995/1‑101 (MQ=255)
                                                                            cgGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTg                          <  1:1985344/101‑1 (MQ=255)
                                                                               tttCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGccc                       >  1:4743349/1‑101 (MQ=255)
                                                                                 tCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAg                     >  1:2552992/1‑101 (MQ=255)
                                                                                 tCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAg                     >  2:1511780/1‑101 (MQ=255)
                                                                                  ccAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGc                    >  1:732903/1‑101 (MQ=255)
                                                                                    aGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGccc                  >  2:1835721/1‑101 (MQ=255)
                                                                                     gcgcCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATTTCATTCGTGCCCAGcccc                 <  2:6574659/101‑1 (MQ=255)
                                                                                       gccgcGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCtt               >  2:5582208/1‑101 (MQ=255)
                                                                                       gccgcGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCtt               <  1:5888791/101‑1 (MQ=255)
                                                                                        ccgcGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTc              <  2:2960077/101‑1 (MQ=255)
                                                                                        ccgcGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTc              >  1:2820998/1‑101 (MQ=255)
                                                                                         cgcgATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCa             <  2:744204/101‑1 (MQ=255)
                                                                                         cgcgATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCa             <  2:5857614/101‑1 (MQ=255)
                                                                                         cgcgATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCa             <  2:1108074/101‑1 (MQ=255)
                                                                                          gcgATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAg            <  2:3727161/101‑1 (MQ=255)
                                                                                           cgATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAgc           >  1:6390187/1‑101 (MQ=255)
                                                                                           cgATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCC.CGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCCGTGCCCAgactggccagc           >  2:6707781/1‑91 (MQ=2)
                                                                                             aTCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAgcgc         >  2:6237355/1‑101 (MQ=255)
                                                                                             aTCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAgcgc         >  2:1263133/1‑101 (MQ=255)
                                                                                              tCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGcgcc        <  1:6774068/101‑1 (MQ=255)
                                                                                              tCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGcgcc        <  2:4128923/101‑1 (MQ=255)
                                                                                              tCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGcgcc        <  2:1299485/101‑1 (MQ=255)
                                                                                              tCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGcgcc        >  2:5666651/1‑101 (MQ=255)
                                                                                                 gcgcCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGCTGTCATTCGTGCCCAGCCCCTTCAGCGCcgcg     >  2:5246317/1‑101 (MQ=255)
                                                                                                 gcgcCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCcgcg     <  2:5371824/101‑1 (MQ=255)
                                                                                                   gcCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGt   >  1:6177286/1‑101 (MQ=255)
                                                                                                    |                                                                                                     
TGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGC.GCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTG  >  NC_014034/678679‑678879

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG