Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 679,222 C→G 100% G1462A (GGG→GCG)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034679,2220C→G100.0% 982.5 267G1462A (GGG→GCG) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (128/139):  ref base (0/0):  total (128/139)

Only 100 of 279 total aligned reads displayed.

CGCCGCCACCGCCGCCGTGCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGGCGGAAATCCCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGCTGCCCGATCCGCGTCGCGCCCAGCGC  >  NC_014034/679125‑679319
                                                                                                 |                                                                                                 
cgccgcCACCGCCGCCGTGCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATcgccc                                                                                                <  1:1600963/101‑1 (MQ=255)
cgccgcCACCGCCGCCGTGCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATcgccc                                                                                                <  2:5158653/101‑1 (MQ=255)
 gccgccACCGCCGCCGTGCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCcg                                                                                               >  1:5318441/1‑101 (MQ=255)
 gccgccACCGCCGCCGTGCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCcg                                                                                               <  1:2899266/101‑1 (MQ=255)
  cggccACCGCGGCCGTGCCCAGCGCCTCGATCTGCCCGGTGGGGAAGGCGGCCCCCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCcgc                                                                                              <  2:2546247/99‑1 (MQ=25)
  ccgccACCGCCGCCGTGCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCcgc                                                                                              <  1:4544139/101‑1 (MQ=255)
    gccACCGCCGCCGTGCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCgcgc                                                                                            <  2:2144027/101‑1 (MQ=255)
      cACCGCCGCCGTGCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGCAAATCGCCCgcgcac                                                                                          >  2:3295992/1‑99 (MQ=255)
       cccgccgccgTGCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGTCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTg                                                                                         <  1:3294945/100‑1 (MQ=255)
       cccgccgccgTGCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTg                                                                                         <  2:4672304/100‑1 (MQ=255)
        ccgccgccgTGCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGa                                                                                        <  2:2067881/101‑1 (MQ=255)
         ccccgccgTGCCCATCGCCTCGATCTCCCCGGTGGTGAAGGCGGCCACCTTCGTGGCGCTTAAGCCGCGCGCCGCCCCGCCCGAAATCGCCCGCGCCTGAt                                                                                       <  2:3317609/99‑1 (MQ=255)
           ccgccgTGCCCAGCGCCTCGATCTGCCCGGTGGTGACGGCGGCCGCCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCg                                                                                     <  2:2004479/101‑1 (MQ=38)
           ccgccgTGCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCg                                                                                     <  1:6870704/101‑1 (MQ=255)
            cgccgTGCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCgg                                                                                    <  2:6796986/101‑1 (MQ=255)
            cgccgTGCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCgg                                                                                    <  2:4527647/101‑1 (MQ=255)
            cgccgTGCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCgg                                                                                    >  2:4140156/1‑101 (MQ=255)
              ccgTGCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTc                                                                                  <  2:1134761/101‑1 (MQ=255)
                gTGCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCga                                                                                <  1:2586281/101‑1 (MQ=255)
                gTGCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCga                                                                                >  1:91628/1‑101 (MQ=255)
                gTGCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCga                                                                                <  1:1476467/101‑1 (MQ=255)
                gTGCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCga                                                                                >  2:707606/1‑101 (MQ=255)
                 tGCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCgag                                                                               <  2:91627/101‑1 (MQ=255)
                  gCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCgaga                                                                              <  1:3239291/101‑1 (MQ=255)
                  gCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCgaga                                                                              <  1:699927/101‑1 (MQ=255)
                   cccAGCGCCTCGTTCTGCCGGGTGGTGAAGGCGCCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGagag                                                                             <  2:4890175/101‑1 (MQ=255)
                   cccAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGagag                                                                             <  2:528525/101‑1 (MQ=255)
                    ccAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAgc                                                                            >  2:4851187/1‑101 (MQ=255)
                    ccAGCGCCTCGATCTGCCCGGTGGTGAAGGCGCCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAgc                                                                            <  2:645939/101‑1 (MQ=255)
                    ccAGCGCCTCGAGCTGCCCGGTTGTAAAGGCGGCCACGTGCGTGGCGCTGAAGCCGCGCGCCGCCGCTCCGAAAATCGCCCGCGCCTGAGCGGTCGAGAgc                                                                            <  1:3700627/101‑1 (MQ=255)
                     cAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAgcg                                                                           >  2:1095347/1‑101 (MQ=255)
                       gcgcCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGcgcc                                                                         <  1:2490542/101‑1 (MQ=255)
                        cgcCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGcgccg                                                                        <  1:6575213/101‑1 (MQ=255)
                         gcCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGcgccgc                                                                       <  2:3786811/101‑1 (MQ=255)
                         gcCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGcgccgc                                                                       >  2:5770618/1‑101 (MQ=255)
                         gcCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGAGGCGCTGAAGCCGCGCGCCGCCGCGCAGGAAATCGCCCGCGCCTGATCGGTCGAGAGcgccgc                                                                       >  1:6325726/1‑101 (MQ=255)
                          ccTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCcgcg                                                                      >  2:6755667/1‑101 (MQ=255)
                          ccTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCcgcg                                                                      >  1:1034596/1‑101 (MQ=255)
                            gcGATCTGCCCGGTGGTGAAGGCGGCCTCCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGAt                                                                    <  1:1272857/100‑1 (MQ=255)
                             cGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATc                                                                   <  1:3488164/101‑1 (MQ=255)
                                  tGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTgcgc                                                              <  2:3111734/101‑1 (MQ=255)
                                   gCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGcgcg                                                             >  1:6088654/1‑101 (MQ=255)
                                   gCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGcgcg                                                             >  1:2416925/1‑101 (MQ=255)
                                   gCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGcgcg                                                             >  1:2097918/1‑101 (MQ=255)
                                   gCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGcgcg                                                             <  1:4107072/101‑1 (MQ=255)
                                     ccGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGt                                                           >  1:6402034/1‑101 (MQ=255)
                                      cGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTc                                                          <  1:4873504/101‑1 (MQ=255)
                                       ggtggtGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCg                                                         >  1:5041815/1‑101 (MQ=255)
                                         gggtgAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGaa                                                       <  2:1924849/100‑1 (MQ=255)
                                          ggtgAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGaaa                                                      >  1:4820096/1‑101 (MQ=255)
                                          ggtgAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGaaa                                                      >  2:6364859/1‑101 (MQ=255)
                                           gtgAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGaaaa                                                     >  2:1043235/1‑101 (MQ=255)
                                            tgAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAg                                                    >  2:1909827/1‑101 (MQ=255)
                                              aaGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGc                                                  >  1:4648785/1‑101 (MQ=255)
                                              aaGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGc                                                  >  2:6180512/1‑101 (MQ=255)
                                                 gcggcCACCTGCGTGGCGCTTAAGCCGCGCGCCGCCGCGCCGGAAAGCGCCCGCGCCTGATCGGTCGATAGCGCCGCGATCTGCGCGGTCGAAAAGGCcgc                                               <  1:642675/101‑1 (MQ=255)
                                                    gcCACCTGCGTGGCGCTGAAGCCGCGCGCCGCTGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGAt                                            <  1:3408364/101‑1 (MQ=255)
                                                    gcCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGAt                                            >  1:1967567/1‑101 (MQ=255)
                                                    gcCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGAt                                            <  1:618859/101‑1 (MQ=255)
                                                     cccccTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATc                                           <  1:836652/98‑1 (MQ=255)
                                                     ccACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATc                                           <  2:2416831/101‑1 (MQ=255)
                                                     ccACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATc                                           <  1:6116797/101‑1 (MQ=255)
                                                      cACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCcgcgccca                                          <  2:5718332/101‑5 (MQ=255)
                                                        ccTGCGTGGCGCTGACGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATcgcc                                        <  2:522220/101‑1 (MQ=255)
                                                          tGCGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATcgccgc                                      <  1:1769226/101‑1 (MQ=255)
                                                            cGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGccgccg                                    >  2:3950245/1‑101 (MQ=255)
                                                            cGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGccgccg                                    >  2:1349170/1‑101 (MQ=255)
                                                            cGTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGccgccg                                    >  1:5662480/1‑101 (MQ=255)
                                                             gTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGt                                   >  1:4293953/1‑101 (MQ=255)
                                                             gTGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGt                                   <  2:442950/101‑1 (MQ=255)
                                                              tgccgcTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTg                                  <  2:4069638/98‑1 (MQ=255)
                                                              tGGCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTg                                  >  2:4997928/1‑101 (MQ=255)
                                                               ggCGCTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGc                                 >  1:1936594/1‑101 (MQ=255)
                                                                 cgcTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGccc                               >  1:3849350/1‑101 (MQ=255)
                                                                  gcTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCa                              >  2:6137813/1‑101 (MQ=255)
                                                                   cTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAg                             >  1:5254791/1‑101 (MQ=255)
                                                                   cTGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAg                             <  2:5619764/101‑1 (MQ=255)
                                                                    tGAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGc                            <  2:275979/101‑1 (MQ=255)
                                                                     gAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGCt                           >  2:6492031/1‑101 (MQ=255)
                                                                     gAAGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGCt                           <  2:6037927/101‑1 (MQ=255)
                                                                      aaGCCGCGCGCCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGCTg                          <  2:4754817/101‑1 (MQ=255)
                                                                             gcgcCGCCGCGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGCTGCCCGATc                   >  1:2859537/1‑101 (MQ=255)
                                                                             cccccgccgcGCGGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGCTGCCCGATc                   <  1:1121580/98‑1 (MQ=38)
                                                                              cgccgccgcGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGCTGCCCGATcc                  <  2:5549067/101‑1 (MQ=255)
                                                                                 cgccgcGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGGGATCGCCGCCGTGCCCAGCTGCCCGATCcgcg               >  1:3551182/1‑101 (MQ=255)
                                                                                  gccgcGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGCTCGCCGCCGTGCCCAGCTGCCCGATCcgcgc              >  2:2577111/1‑100 (MQ=255)
                                                                                  gccgcGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGCTGCCCGATCCGCGt              >  2:2944406/1‑101 (MQ=255)
                                                                                   ccgcGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGCTGCCCGATCCGCGTc             >  2:1200803/1‑101 (MQ=255)
                                                                                   ccgcGCCGGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGCTGCCCGATCCGCGTc             >  2:7216/1‑101 (MQ=255)
                                                                                     gcgccgGAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGCTGCCCGATCCGCGTcgc           <  1:1095382/101‑1 (MQ=255)
                                                                                      cgccggAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGCTGCCCGATCCGCGTcgcg          <  2:5024735/98‑1 (MQ=255)
                                                                                         cggAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGCTGCCCGATCCGCGTCGCGccc       <  1:3945016/101‑1 (MQ=255)
                                                                                          ggAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGCTGCCCGATCCGCGTCGCGCCCa      >  1:3328352/1‑101 (MQ=255)
                                                                                          cgAAATCGCCCGCGCCTGCTCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGCTGCCCGATCCGCGTCGCGCCCa      <  1:2806849/100‑1 (MQ=255)
                                                                                           gAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGCTGCCCGATCCGCGTCGCGCCCAg     <  1:3806949/101‑1 (MQ=255)
                                                                                           gAAATCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGCTGCCCGATCCGCGTCGCGCCCAg     >  2:1003315/1‑101 (MQ=255)
                                                                                            aaaTCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGCTGCCCGATCCGCGTCGCGCCCAgc    <  2:3439042/101‑1 (MQ=255)
                                                                                             aaTCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGCTGCCCGATCCGCGTCGCGCCCAgcg   >  1:2627965/1‑101 (MQ=255)
                                                                                             aaTCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGCTGCCCGATCCGCGTCGCGCCCAgcg   >  2:3689593/1‑101 (MQ=255)
                                                                                              aTCGCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGCTGCCCGATCCGCGTCGCGCCCAgcgc  >  1:5115186/1‑101 (MQ=255)
                                                                                                 |                                                                                                 
CGCCGCCACCGCCGCCGTGCCCAGCGCCTCGATCTGCCCGGTGGTGAAGGCGGCCACCTGCGTGGCGCTGAAGCCGCGCGCCGCCGCGGCGGAAATCCCCCGCGCCTGATCGGTCGAGAGCGCCGCGATCTGCGCGGTCGAAAAGGCCGCGATCGCCGCCGTGCCCAGCTGCCCGATCCGCGTCGCGCCCAGCGC  >  NC_014034/679125‑679319

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG