Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,459 G→A 21.0% I716I (ATC→ATT RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,4590G→A21.0% 170.1 ‑0.9 ‑0.2299I716I (ATC→ATTRCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (29/34):  ref base (125/111):  total (154/145)
Fisher's exact test strand distribution p-value = 3.95e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.00e-01

Only 100 of 328 total aligned reads displayed.

CCGCGATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCG  >  NC_014034/681359‑681559
                                                                                                    |                                                                                                     
ccgcGATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCcgcg                                                                                                       >  2:213542/1‑101 (MQ=255)
ccgcGATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCcgcg                                                                                                       <  2:1481874/101‑1 (MQ=255)
    gATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGactg                                                                                                   >  2:353519/1‑98 (MQ=255)
     aTCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCtc                                                                                                  >  1:2041747/1‑100 (MQ=255)
     aTCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTg                                                                                                  >  1:3984297/1‑101 (MQ=255)
     aTCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTg                                                                                                  <  2:5288809/101‑1 (MQ=255)
     aTCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTg                                                                                                  <  1:6629198/101‑1 (MQ=255)
      tCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGc                                                                                                 <  1:6360171/101‑1 (MQ=255)
       cTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGGGCCAGATCCGCCGCCTCGAGCGCCGCGATGTgtg                                                                                                >  2:2263486/1‑99 (MQ=255)
       cTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTCCg                                                                                                >  2:2766972/1‑101 (MQ=255)
        tGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGt                                                                                               <  2:1097323/101‑1 (MQ=255)
         gcgcgcCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTg                                                                                              >  1:5450195/1‑101 (MQ=255)
           gcgcCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCt                                                                                            <  1:3564946/101‑1 (MQ=255)
            cgcCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTg                                                                                           <  1:3398863/101‑1 (MQ=255)
            cgcCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTg                                                                                           <  2:3481604/101‑1 (MQ=255)
            cgcCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTg                                                                                           <  2:5945878/101‑1 (MQ=255)
             gcCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTgg                                                                                          >  1:6517200/1‑101 (MQ=255)
               cTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTc                                                                                        >  2:4407269/1‑101 (MQ=255)
               cTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTc                                                                                        <  1:1560429/101‑1 (MQ=255)
                tGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCa                                                                                       <  1:4248609/101‑1 (MQ=255)
                tGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCa                                                                                       >  2:6444112/1‑101 (MQ=255)
                tGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCa                                                                                       >  2:853969/1‑101 (MQ=255)
                tGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCa                                                                                       >  1:2868541/1‑101 (MQ=255)
                 gAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAg                                                                                      >  1:4764078/1‑101 (MQ=255)
                    aGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGGGCTGGTCAgcgc                                                                                   <  1:6320364/101‑1 (MQ=255)
                          gCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAt                                                                             <  1:4650493/101‑1 (MQ=255)
                          gCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAt                                                                             <  1:2263511/101‑1 (MQ=255)
                               cGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTgcg                                                                       >  2:6094589/1‑101 (MQ=255)
                                gTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTgcgc                                                                      >  2:4209258/1‑101 (MQ=255)
                                  ggCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCg                                                                    >  1:4052557/1‑101 (MQ=255)
                                  ggCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCg                                                                    >  1:5745233/1‑101 (MQ=255)
                                      gAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGcc                                                                >  1:1660882/1‑101 (MQ=255)
                                       agcccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGccc                                                               >  2:1239321/1‑101 (MQ=255)
                                       agcccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGccc                                                               <  1:7063158/101‑1 (MQ=255)
                                         cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAg                                                             <  1:6981907/101‑1 (MQ=255)
                                         cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAg                                                             <  1:1044410/101‑1 (MQ=255)
                                               ccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACgcgc                                                       <  1:4407491/101‑1 (MQ=255)
                                                cGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGcgcc                                                      <  1:2626774/101‑1 (MQ=255)
                                                 ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGcgccg                                                     >  2:5052794/1‑101 (MQ=255)
                                                  gagaGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGcgccgc                                                    >  1:732015/1‑101 (MQ=255)
                                                  gagaGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGcgccgc                                                    >  2:4820244/1‑101 (MQ=255)
                                                   agagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCgccgcc                                                   <  2:2083177/101‑1 (MQ=255)
                                                   agagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCgccgcc                                                   <  2:6879587/101‑1 (MQ=255)
                                                     agCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTg                                                 <  2:3736554/101‑1 (MQ=255)
                                                     agCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTg                                                 <  1:4129236/101‑1 (MQ=255)
                                                        gcCGTCGCGCTGATCTGCGCCGGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTgcgc                                              <  2:5858508/101‑1 (MQ=255)
                                                         ccGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGcc                                             <  1:1426059/101‑1 (MQ=255)
                                                            tCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtc                                          <  2:311680/101‑1 (MQ=255)
                                                            gcgcgCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtc                                          <  2:542454/100‑1 (MQ=255)
                                                              gcgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtcgt                                        >  2:565433/1‑101 (MQ=255)
                                                               cgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCgtcgtc                                       <  1:5917029/101‑1 (MQ=255)
                                                                gcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCa                                      <  1:2979479/101‑1 (MQ=255)
                                                                gcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCa                                      <  2:6743848/101‑1 (MQ=255)
                                                                  tGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgc                                    >  1:5982117/1‑101 (MQ=255)
                                                                  tGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgc                                    <  1:6094901/101‑1 (MQ=255)
                                                                      cTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCa                                <  1:3963508/101‑1 (MQ=255)
                                                                       tGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCAc                               >  1:3386276/1‑101 (MQ=255)
                                                                       cgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCAc                               <  1:231204/97‑1 (MQ=2)
                                                                         caccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGa                             >  1:3859458/3‑101 (MQ=2)
                                                                         caccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGa                             >  2:1912849/3‑101 (MQ=2)
                                                                          gcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGAt                            >  2:632557/1‑101 (MQ=255)
                                                                          gcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGAt                            <  1:529540/101‑1 (MQ=255)
                                                                          gcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGGGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGAt                            <  2:3985633/101‑1 (MQ=255)
                                                                          cccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGAt                            <  2:1850332/100‑1 (MQ=9)
                                                                           ccAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGGCAGCGCCACGATg                           >  2:7057178/1‑101 (MQ=255)
                                                                            cAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGt                          <  1:182541/101‑1 (MQ=255)
                                                                             aGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGATGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc                         >  1:3713877/1‑101 (MQ=255)
                                                                              gATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                        >  2:1715029/1‑101 (MQ=255)
                                                                              gATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                        >  1:3313505/1‑101 (MQ=255)
                                                                                tCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATc                      >  2:552712/1‑101 (MQ=255)
                                                                                cccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATc                      <  1:991507/100‑1 (MQ=255)
                                                                                  cgcggcCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCgg                    <  2:5009225/101‑1 (MQ=255)
                                                                                  cgccgcCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCgg                    >  1:1704400/1‑101 (MQ=255)
                                                                                  cgccgcCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGGCATCgg                    >  2:4027826/1‑101 (MQ=255)
                                                                                   gcggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGt                   <  1:1772036/92‑1 (MQ=9)
                                                                                     ggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGc                 <  2:4929670/94‑1 (MQ=9)
                                                                                      gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                >  2:3307890/7‑101 (MQ=9)
                                                                                      gccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACTCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                <  2:3394130/101‑1 (MQ=255)
                                                                                       tttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccg               >  2:1315635/6‑100 (MQ=2)
                                                                                       tttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccg               <  1:6054484/96‑2 (MQ=9)
                                                                                       ccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCa               >  1:2363580/1‑101 (MQ=255)
                                                                                       ccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCa               >  2:2987071/1‑101 (MQ=255)
                                                                                        ttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccga              >  2:2714753/5‑99 (MQ=2)
                                                                                         tCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaaa             >  2:2378513/1‑101 (MQ=255)
                                                                                         tCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaaa             <  1:6253503/101‑1 (MQ=255)
                                                                                           gAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGc           <  1:6724316/101‑1 (MQ=255)
                                                                                           gAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGc           <  1:2767272/101‑1 (MQ=255)
                                                                                           caGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgaggc           >  1:1293356/2‑96 (MQ=9)
                                                                                           caGCGCCGCAACCTGCGTGCTCGACAGCGC.CAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgaggc           >  1:3190736/2‑96 (MQ=2)
                                                                                            aGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGGCAGCGCCACGCTGTCATCGGCGCCAAAGcc          >  1:556632/1‑101 (MQ=255)
                                                                                            aGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc          >  2:6387363/1‑101 (MQ=9)
                                                                                            aGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc          <  1:2644158/101‑1 (MQ=9)
                                                                                              cgccgcAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccct        <  1:5089339/101‑3 (MQ=9)
                                                                                              cgccgcAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccct        <  1:3637032/101‑3 (MQ=9)
                                                                                               gccgcAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccctt       >  1:1261454/1‑98 (MQ=2)
                                                                                                   cgATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATc   >  1:4355056/1‑101 (MQ=255)
                                                                                                    gATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg  >  1:4527112/1‑101 (MQ=255)
                                                                                                    gATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg  >  1:4946297/1‑101 (MQ=255)
                                                                                                    gATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg  >  1:678763/1‑101 (MQ=255)
                                                                                                    gATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg  >  2:4611648/1‑101 (MQ=255)
                                                                                                    |                                                                                                     
CCGCGATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCG  >  NC_014034/681359‑681559

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG