Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,482 C→G 21.0% G709R (GGC→CGA)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,4820C→G21.0% 169.9 ‑2.1 ‑0.7309G709R (GGC→CGC) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (27/37):  ref base (129/113):  total (159/150)
Fisher's exact test strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.86e-01

Only 100 of 326 total aligned reads displayed.

CCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACC  >  NC_014034/681382‑681582
                                                                                                    |                                                                                                     
ccGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGcc                                                                                                       <  1:4395944/101‑1 (MQ=255)
 cGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCg                                                                                                      >  1:175494/1‑101 (MQ=255)
 cGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCg                                                                                                      >  1:562620/1‑101 (MQ=255)
  ggCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGa                                                                                                     >  2:2623441/1‑101 (MQ=255)
   gCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAt                                                                                                    <  1:4650493/101‑1 (MQ=255)
   gCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAt                                                                                                    <  1:2263511/101‑1 (MQ=255)
      aTCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTg                                                                                                >  1:362498/1‑101 (MQ=255)
        cGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTgcg                                                                                              >  2:1328201/1‑101 (MQ=255)
         gTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTgcgc                                                                                             >  1:60321/1‑101 (MQ=255)
          tGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGcc                                                                                            >  2:6584258/1‑101 (MQ=255)
          tGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGcc                                                                                            <  2:2386848/101‑1 (MQ=255)
             ccGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAg                                                                                         <  1:4209453/101‑1 (MQ=255)
              cGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGc                                                                                        >  2:4129041/1‑101 (MQ=255)
                agcccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGccc                                                                                      >  2:3579797/1‑101 (MQ=255)
                  cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAg                                                                                    <  1:1044410/101‑1 (MQ=255)
                  cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAg                                                                                    <  2:6255120/101‑1 (MQ=255)
                         cGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGcgcc                                                                             <  1:2626774/101‑1 (MQ=255)
                         cGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGcgcc                                                                             <  2:5468187/101‑1 (MQ=255)
                           gagaGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGcgccgc                                                                           >  2:4820244/1‑101 (MQ=255)
                           gagaGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGcgccgc                                                                           >  2:1009445/1‑101 (MQ=255)
                               gcgcCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTgc                                                                       <  2:5185693/101‑1 (MQ=255)
                               gcgcCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTgc                                                                       <  1:6691583/101‑1 (MQ=255)
                                 tcctcggtgcTGATCTGCGCCAGATTCGCCGGCTCGAGCGCCGCGACCTGCGTGCTGTTCAGCGCGCAGAT.CTGCGCCCAGCCGAGATGCGCTGCCTgctc                                                                     >  2:3516807/9‑99 (MQ=255)
                                 gcCGTCGCGCTGATCTGCGCCGGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTgcgc                                                                     <  2:5858508/101‑1 (MQ=255)
                                 gcCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTgcgc                                                                     <  2:538015/101‑1 (MQ=255)
                                    gTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgt                                                                  >  1:1665798/1‑101 (MQ=255)
                                       gcgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtcgt                                                               >  2:565433/1‑101 (MQ=255)
                                        cgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCgtcgtc                                                              >  1:3846750/1‑101 (MQ=255)
                                        cgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCgtcgtc                                                              <  2:3363780/101‑1 (MQ=255)
                                         gcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCa                                                             <  1:2979479/101‑1 (MQ=255)
                                         gcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCa                                                             <  2:6743848/101‑1 (MQ=255)
                                           tGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgc                                                           <  1:6094901/101‑1 (MQ=255)
                                           ggagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAgc                                                           <  2:4450011/92‑1 (MQ=2)
                                           ggagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAgc                                                           <  2:5049895/92‑1 (MQ=2)
                                            gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCGGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcg                                                          <  1:1267621/101‑1 (MQ=255)
                                            gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcg                                                          >  2:1588922/1‑101 (MQ=255)
                                            gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcg                                                          >  1:5960451/1‑101 (MQ=255)
                                             aTCTGCGCCAGAACCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcgc                                                         >  1:5079241/1‑101 (MQ=255)
                                              tCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGcc                                                        <  1:1639376/101‑1 (MQ=255)
                                              gccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGcc                                                        <  1:1785488/95‑1 (MQ=2)
                                               ccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCa                                                       <  1:6327368/96‑1 (MQ=2)
                                               cTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCa                                                       <  1:3963508/101‑1 (MQ=255)
                                               cTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCa                                                       >  2:4971896/1‑101 (MQ=255)
                                                tGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCAc                                                      <  2:747290/101‑1 (MQ=255)
                                                tGCGCCAGACCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCAc                                                      <  1:6584598/101‑1 (MQ=255)
                                                cgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCAc                                                      <  1:231204/97‑1 (MQ=2)
                                                 gcgcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCGACg                                                     >  2:2115011/1‑101 (MQ=255)
                                                   gcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGGGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGAt                                                   <  2:3985633/101‑1 (MQ=255)
                                                      aGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc                                                >  1:4105284/1‑101 (MQ=255)
                                                      aGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc                                                <  1:2743221/101‑1 (MQ=255)
                                                      aGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGATGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc                                                >  1:3713877/1‑101 (MQ=255)
                                                       gATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                                               >  1:3313505/1‑101 (MQ=255)
                                                       gATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                                               <  2:3034504/101‑1 (MQ=255)
                                                       gATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                                               >  1:4411883/1‑101 (MQ=255)
                                                       gATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                                               >  2:6004962/1‑101 (MQ=255)
                                                        aTCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCCCGATGTCAt                                              >  2:4343735/1‑101 (MQ=255)
                                                        aTCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCAt                                              <  1:5278396/101‑1 (MQ=255)
                                                           ggcggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCgg                                           >  2:4879981/11‑101 (MQ=9)
                                                           cgccgcCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGGCATCgg                                           >  2:4027826/1‑101 (MQ=255)
                                                             ccgccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTg                                         <  2:1498806/101‑1 (MQ=255)
                                                               gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                       >  1:7097368/7‑101 (MQ=9)
                                                               cccTCGAGCGCCGCGATCTGCGTGCTGGTCTGCGCCCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                       <  2:2033015/100‑1 (MQ=255)
                                                                tttccaGCGCCGCAGCCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccg                                      <  2:1109432/96‑2 (MQ=9)
                                                                ccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCa                                      >  2:2987071/1‑101 (MQ=255)
                                                                ccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCa                                      >  1:2363580/1‑101 (MQ=255)
                                                                 ttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccga                                     >  1:5463988/5‑99 (MQ=2)
                                                                  tCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaaa                                    <  1:3451612/101‑1 (MQ=255)
                                                                  tCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaaa                                    <  1:6253503/101‑1 (MQ=255)
                                                                   cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                                   >  2:1899488/1‑101 (MQ=255)
                                                                   cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                                   <  2:3254667/101‑1 (MQ=255)
                                                                    gAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGc                                  <  1:2767272/101‑1 (MQ=255)
                                                                    gAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGc                                  <  1:5895809/101‑1 (MQ=255)
                                                                    caGCGCCGCAACCTGCGTGCTCGACAGCGC.CAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgaggc                                  >  1:3190736/2‑96 (MQ=2)
                                                                     aGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                 <  1:2644158/101‑1 (MQ=9)
                                                                         ccgcAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcccttg                             <  1:6994464/101‑5 (MQ=2)
                                                                         ccgcAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcccttg                             <  1:68503/101‑5 (MQ=2)
                                                                           gcgATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt                           <  2:6428237/101‑1 (MQ=255)
                                                                             tgtCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                         <  2:6922615/99‑1 (MQ=255)
                                                                             gATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                         >  1:4527112/1‑101 (MQ=255)
                                                                             gATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                         >  2:4611648/1‑101 (MQ=255)
                                                                             gATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                         >  1:4946297/1‑101 (MQ=255)
                                                                               tCTGCTTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgcc                       <  2:592949/101‑1 (MQ=255)
                                                                               tCTGCGTGCGGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgcc                       <  1:5698647/101‑1 (MQ=255)
                                                                               ccTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGTCACGATGTCATCGGTGCCGAGGCACCTGATcgca                       >  2:2222327/2‑100 (MQ=2)
                                                                                 tGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccgc                     <  2:3443149/101‑1 (MQ=255)
                                                                                  gCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCACCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATcgcattg                    <  2:1259152/101‑5 (MQ=0)
                                                                                    gggCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGt                  <  1:131929/99‑1 (MQ=255)
                                                                                    gTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGt                  >  2:5580703/1‑101 (MQ=255)
                                                                                      gCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGc                >  2:2543454/1‑101 (MQ=255)
                                                                                       cTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCNNNcn               >  1:6741340/1‑100 (MQ=255)
                                                                                       cTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGcc               >  1:3065129/1‑101 (MQ=255)
                                                                                         ggTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCa             <  1:63946/101‑1 (MQ=255)
                                                                                          gTCAGCCCCCCGAC.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAg            <  2:2893659/101‑1 (MQ=255)
                                                                                            cAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcg          >  2:1781979/1‑101 (MQ=255)
                                                                                            cAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGACAAAGCAGCGGATCGCCGCCGTGccagatc          >  1:1222562/1‑96 (MQ=255)
                                                                                             aGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcgc         >  1:1886389/1‑101 (MQ=255)
                                                                                             aGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcgc         >  1:919335/1‑101 (MQ=255)
                                                                                                  gcCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCa    <  2:632945/101‑1 (MQ=255)
                                                                                                   ccGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCAc   >  2:7046074/1‑101 (MQ=255)
                                                                                                    cGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCAcc  <  1:4003680/101‑1 (MQ=255)
                                                                                                    |                                                                                                     
CCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACC  >  NC_014034/681382‑681582

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG