Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,485 T→G 21.3% M708L (ATC→CTC)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,4850T→G21.3% 186.4 ‑1.8 ‑0.4311M708L (ATC→CTC) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (29/37):  ref base (132/112):  total (162/149)
Fisher's exact test strand distribution p-value = 1.65e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.64e-01

Only 100 of 330 total aligned reads displayed.

GCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTC  >  NC_014034/681385‑681585
                                                                                                     |                                                                                                    
gCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.At                                                                                                      <  1:2263511/101‑1 (MQ=255)
  caccGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCt                                                                                                    <  2:349921/98‑1 (MQ=255)
   aTCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTg                                                                                                   >  1:4124744/1‑101 (MQ=255)
      gTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTgcgc                                                                                                >  1:60321/1‑101 (MQ=255)
      gTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTgcgc                                                                                                <  1:4630717/101‑1 (MQ=255)
       tGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGcc                                                                                               >  2:6584258/1‑101 (MQ=255)
           cGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGc                                                                                           <  2:1312117/101‑1 (MQ=255)
           cGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGc                                                                                           >  2:1722509/1‑101 (MQ=255)
             agcccagcccGGAGAGCGCCGTCGCGTTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGccc                                                                                         <  2:4627686/101‑1 (MQ=255)
             agcccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGccc                                                                                         <  1:7063158/101‑1 (MQ=255)
              gcccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCa                                                                                        <  1:235004/101‑1 (MQ=255)
               cccatcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAg                                                                                       <  1:3336483/101‑1 (MQ=255)
               cccagccgGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAg                                                                                       <  2:4852400/101‑1 (MQ=255)
               cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAg                                                                                       <  2:6255120/101‑1 (MQ=255)
               cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAg                                                                                       <  1:1044410/101‑1 (MQ=255)
               cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGCGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAg                                                                                       <  1:5005407/101‑1 (MQ=255)
                ccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGa                                                                                      >  1:3007125/1‑101 (MQ=255)
                 cagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGAc                                                                                     <  1:4935718/101‑1 (MQ=255)
                     ccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACgcgc                                                                                 <  1:4407491/101‑1 (MQ=255)
                      cGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGcgcc                                                                                <  1:2626774/101‑1 (MQ=255)
                       ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGcgccg                                                                               >  2:5052794/1‑101 (MQ=255)
                        gagaGCGCCGTCGCGTTGATCTGCGCCAGATCCCCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCACCGCGCCG.ATCTGCGCCGAGCCGAGACGcgccgc                                                                              <  2:5828699/101‑1 (MQ=37)
                         agagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCgccgcc                                                                             <  2:2083177/101‑1 (MQ=255)
                         agagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCgccgcc                                                                             >  1:6106615/1‑101 (MQ=255)
                           agCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTg                                                                           <  2:3736554/101‑1 (MQ=255)
                            gcgcCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTgc                                                                          <  1:6691583/101‑1 (MQ=255)
                               ccGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGcc                                                                       <  1:3138634/101‑1 (MQ=255)
                                 gTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgt                                                                     >  1:1665798/1‑101 (MQ=255)
                                 gTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgt                                                                     <  2:2749130/101‑1 (MQ=255)
                                 gTCCCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgt                                                                     <  2:2953302/101‑1 (MQ=255)
                                  gcgcgCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtc                                                                    <  2:542454/100‑1 (MQ=255)
                                    gcgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtcgt                                                                  >  2:565433/1‑101 (MQ=255)
                                     cgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCgtcgtc                                                                 >  2:211815/1‑101 (MQ=255)
                                     cgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCgtcgtc                                                                 >  1:3846750/1‑101 (MQ=255)
                                     cgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCgtcgtc                                                                 <  1:5917029/101‑1 (MQ=255)
                                      gcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCa                                                                <  2:6743848/101‑1 (MQ=255)
                                        tGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgc                                                              >  2:5329462/1‑101 (MQ=255)
                                         gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCGGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcg                                                             <  1:1267621/101‑1 (MQ=255)
                                         gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcg                                                             >  1:5960451/1‑101 (MQ=255)
                                           tCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGcc                                                           >  2:4017278/1‑101 (MQ=255)
                                           tCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGcc                                                           <  1:1639376/101‑1 (MQ=255)
                                            ccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCa                                                          <  1:6327368/96‑1 (MQ=2)
                                            cTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCa                                                          <  1:3963508/101‑1 (MQ=255)
                                             tGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCAc                                                         <  2:747290/101‑1 (MQ=255)
                                             tGCGCCAGACCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCAc                                                         <  1:6584598/101‑1 (MQ=255)
                                              gcgcCAGATCCGTCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCTAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACg                                                        >  1:5097759/1‑101 (MQ=255)
                                                gcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGAt                                                      >  2:632557/1‑101 (MQ=255)
                                                cccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGAt                                                      <  2:4086836/100‑1 (MQ=9)
                                                  cAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGt                                                    <  1:182541/101‑1 (MQ=255)
                                                    gATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                                                  >  2:6004962/1‑101 (MQ=255)
                                                    gATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                                                  <  2:3034504/101‑1 (MQ=255)
                                                     aTCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCAt                                                 <  1:2498059/101‑1 (MQ=255)
                                                      tCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATc                                                <  1:3747574/101‑1 (MQ=255)
                                                      cccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATc                                                <  1:991507/100‑1 (MQ=255)
                                                        cgcggcCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCgg                                              <  2:5009225/101‑1 (MQ=255)
                                                         gcggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGt                                             <  1:1772036/92‑1 (MQ=9)
                                                          cggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTg                                            >  2:3578396/9‑101 (MQ=9)
                                                           ggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGc                                           <  2:4929670/94‑1 (MQ=9)
                                                            gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                          <  1:5462613/95‑1 (MQ=9)
                                                            gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                          <  1:4482228/95‑1 (MQ=9)
                                                            gccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACTCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                          <  2:3394130/101‑1 (MQ=255)
                                                            gccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                          <  1:6257624/101‑1 (MQ=255)
                                                                cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                                      >  2:1899488/1‑101 (MQ=255)
                                                                cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                                      <  2:3254667/101‑1 (MQ=255)
                                                                  aGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGGCAGCGCCACGCTGTCATCGGCGCCAAAGcc                                    >  1:556632/1‑101 (MQ=255)
                                                                  aGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                    <  1:2644158/101‑1 (MQ=9)
                                                                  aGCGCCGCAACCTGCGTGCTCGACAGCGC.CAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                    >  1:3492027/1‑101 (MQ=2)
                                                                    cgccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccct                                  <  1:3637032/101‑3 (MQ=9)
                                                                     gccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccctt                                 <  1:2847488/101‑4 (MQ=9)
                                                                      ccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcccttg                                <  1:68503/101‑5 (MQ=2)
                                                                      ccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcccttg                                <  1:6994464/101‑5 (MQ=2)
                                                                        gcgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt                              <  2:6428237/101‑1 (MQ=255)
                                                                         cgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATc                             >  2:1395423/1‑101 (MQ=255)
                                                                          tgtCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                            <  2:6922615/99‑1 (MQ=255)
                                                                          gATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                            >  1:6738841/1‑101 (MQ=255)
                                                                           aTCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgc                           >  2:2449926/1‑101 (MQ=255)
                                                                            tCTGCTTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgcc                          <  2:592949/101‑1 (MQ=255)
                                                                            tCTGCGTGCGGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgcc                          <  1:5698647/101‑1 (MQ=255)
                                                                                 gggCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGt                     <  1:131929/99‑1 (MQ=255)
                                                                                 gTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGt                     >  1:2746274/1‑101 (MQ=255)
                                                                                 gTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGt                     >  2:5580703/1‑101 (MQ=255)
                                                                                 gTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGGGCCAAAGCCGCGGATCGccgccgg                     >  2:2261239/1‑100 (MQ=255)
                                                                                  tGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTg                    >  2:5198170/1‑101 (MQ=255)
                                                                                    gatctCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGcc                  <  2:1222514/97‑1 (MQ=255)
                                                                                    cTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGcc                  >  2:4362090/1‑101 (MQ=255)
                                                                                     tGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGccc                 >  2:2606977/1‑101 (MQ=255)
                                                                                      ggTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCa                <  1:63946/101‑1 (MQ=255)
                                                                                       gTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAg               <  2:1503239/101‑1 (MQ=255)
                                                                                         cAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcg             >  2:4473767/1‑101 (MQ=255)
                                                                                         cAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcg             >  2:4130423/1‑101 (MQ=255)
                                                                                         cAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcg             >  2:1781979/1‑101 (MQ=255)
                                                                                         cAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGACAAAGCAGCGGATCGCCGCCGTGccagatc             >  1:1222562/1‑96 (MQ=255)
                                                                                          aGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcgc            >  1:919335/1‑101 (MQ=255)
                                                                                          aGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcgc            >  1:1886389/1‑101 (MQ=255)
                                                                                          aGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcgc            >  1:4240491/1‑101 (MQ=255)
                                                                                            cgcgcCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGcgccg          <  1:3219263/101‑1 (MQ=255)
                                                                                              cgcCG.ATCTGCGCCGAACCCAAACGCGCCGCCTGCGGCGTCGTCAGCGCCGCGATGTCATCGGTGCCAAAGCCGCGGATCACCGCCGGGCCCAGCgccgcc        <  2:1050688/101‑1 (MQ=38)
                                                                                                ccG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCAc      >  2:7046074/1‑101 (MQ=255)
                                                                                                 cG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCAcc     <  1:4003680/101‑1 (MQ=255)
                                                                                                  g.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCg    <  2:3313389/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
GCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTC  >  NC_014034/681385‑681585

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG