Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,503 G→C 21.7% R702G (CGT→GGT)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,5030G→C21.7% 176.9 ‑1.8 ‑1.7304R702G (CGT→GGT) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (29/37):  ref base (129/108):  total (158/146)
Fisher's exact test strand distribution p-value = 1.63e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.97e-02

Only 100 of 322 total aligned reads displayed.

AGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGC  >  NC_014034/681403‑681602
                                                                                                     |                                                                                                   
agcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGAcg                                                                                                     >  1:6480073/1‑101 (MQ=255)
        gagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCt                                                                                             >  1:6726803/1‑101 (MQ=255)
         agCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTg                                                                                            <  1:3795200/101‑1 (MQ=255)
         agCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTg                                                                                            <  2:3736554/101‑1 (MQ=255)
            gcCGTCGCGCTGATCTGCGCCGGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTgcgc                                                                                         <  2:5858508/101‑1 (MQ=255)
              cGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCcg                                                                                       >  1:1503299/1‑101 (MQ=255)
                gcgcgCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtc                                                                                     <  2:542454/100‑1 (MQ=255)
                    gcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCa                                                                                 >  2:5641927/1‑101 (MQ=255)
                     cTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAg                                                                                >  2:4017661/1‑101 (MQ=255)
                      tGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgc                                                                               >  2:4119247/1‑101 (MQ=255)
                      tGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgc                                                                               >  2:5329462/1‑101 (MQ=255)
                      ggagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAgc                                                                               <  1:5921145/92‑1 (MQ=2)
                      ggagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAgc                                                                               <  2:4450011/92‑1 (MQ=2)
                       gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcg                                                                              >  1:5960451/1‑101 (MQ=255)
                       gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcg                                                                              >  2:1588922/1‑101 (MQ=255)
                         tCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGcc                                                                            >  1:615640/1‑101 (MQ=255)
                         gccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGcc                                                                            <  1:256566/95‑1 (MQ=2)
                         gccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGcc                                                                            <  1:1785488/95‑1 (MQ=2)
                          cTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCa                                                                           >  1:6321273/1‑101 (MQ=255)
                           cgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCAc                                                                          <  1:231204/97‑1 (MQ=2)
                           cgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCAc                                                                          <  2:5526735/97‑1 (MQ=2)
                            gcgcCAGATCCGTCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCTAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACg                                                                         >  1:5097759/1‑101 (MQ=255)
                            gcgcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCGACg                                                                         >  2:2115011/1‑101 (MQ=255)
                             caccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGa                                                                        >  1:3859458/3‑101 (MQ=2)
                             caccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGa                                                                        >  1:1105011/3‑101 (MQ=2)
                               ccAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGGCAGCGCCACGATg                                                                      >  2:7057178/1‑101 (MQ=255)
                                 aGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGGCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTTCGCCGTCGCACGCGTCTCGATGTc                                                                    >  2:1110867/1‑101 (MQ=25)
                                 aGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGATGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc                                                                    >  1:3713877/1‑101 (MQ=255)
                                  gATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                                                                   >  1:4411883/1‑101 (MQ=255)
                                  gATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                                                                   >  1:3313505/1‑101 (MQ=255)
                                   aTCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCCCGATGTCAt                                                                  >  2:4343735/1‑101 (MQ=255)
                                    tCCGCCCCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCAAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATc                                                                 <  1:7029558/101‑1 (MQ=255)
                                      ggcggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCgg                                                               >  2:2729381/11‑101 (MQ=9)
                                      cgcggcCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCgg                                                               <  2:5009225/101‑1 (MQ=255)
                                      cgccgcCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCgg                                                               >  1:1704400/1‑101 (MQ=255)
                                      cgccgcCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCgg                                                               >  1:2695396/1‑101 (MQ=255)
                                        cggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTg                                                             >  2:3578396/9‑101 (MQ=9)
                                        cggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTg                                                             >  2:1037426/9‑101 (MQ=9)
                                         ggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGc                                                            <  2:4929670/94‑1 (MQ=9)
                                         ggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGc                                                            >  2:2907028/8‑101 (MQ=9)
                                          gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                                           <  2:1879065/95‑1 (MQ=12)
                                          gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                                           <  1:3981151/95‑1 (MQ=9)
                                          gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                                           <  1:5462613/95‑1 (MQ=9)
                                          gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                                           <  1:4482228/95‑1 (MQ=9)
                                          gccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACTCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                                           <  2:3394130/101‑1 (MQ=255)
                                           tttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccg                                                          <  1:6054484/96‑2 (MQ=9)
                                           tttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccg                                                          >  2:5107543/6‑100 (MQ=2)
                                           ccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCa                                                          >  1:2363580/1‑101 (MQ=255)
                                             tccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgag                                                        >  1:586661/4‑98 (MQ=2)
                                             tccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgag                                                        <  1:6960136/98‑4 (MQ=9)
                                             tCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaaa                                                        <  1:3451612/101‑1 (MQ=255)
                                              cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                                                       <  2:3254667/101‑1 (MQ=255)
                                               gAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGc                                                      >  2:6453958/1‑101 (MQ=255)
                                               caGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgaggc                                                      <  2:6281244/100‑6 (MQ=9)
                                               caGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgaggc                                                      >  1:1293356/2‑96 (MQ=9)
                                               caGCGCCGCAACCTGCGTGCTCGACAGCGC.CAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgaggc                                                      >  1:3190736/2‑96 (MQ=2)
                                                aGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGGCAGCGCCACGCTGTCATCGGCGCCAAAGcc                                                     >  1:556632/1‑101 (MQ=255)
                                                 gcgcCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGCGCCGAGGccc                                                    <  1:6624869/101‑2 (MQ=9)
                                                   gccgcAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccctt                                                  >  1:1261454/1‑98 (MQ=2)
                                                   gccgcAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccctt                                                  <  1:2847488/101‑4 (MQ=9)
                                                    ccgcAACCTGCGTGCTCGCCAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcccttg                                                 <  2:6839919/101‑5 (MQ=2)
                                                       cgATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATc                                              >  1:4355056/1‑101 (MQ=255)
                                                        tgtCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                                             <  2:6922615/99‑1 (MQ=255)
                                                        gATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                                             >  1:678763/1‑101 (MQ=255)
                                                         aTCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgc                                            >  2:2449926/1‑101 (MQ=255)
                                                          tCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgcc                                           <  2:3007016/101‑1 (MQ=255)
                                                          ccTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGTCACGATGTCATCGGTGCCGAGGCACCTGATcgca                                           >  2:2222327/2‑100 (MQ=2)
                                                           cTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccg                                          <  1:4957250/101‑1 (MQ=255)
                                                           cTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCACCGGTGCCGAGGCCCTTGATcgcat                                          >  1:4553372/1‑99 (MQ=2)
                                                             gCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCgccgcc                                        <  2:1441887/101‑1 (MQ=255)
                                                             gCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCACCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATcgcattg                                        <  2:1259152/101‑5 (MQ=0)
                                                               gggCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGt                                      <  1:131929/99‑1 (MQ=255)
                                                               gTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGt                                      >  1:2746274/1‑101 (MQ=255)
                                                                tGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTg                                     >  2:5198170/1‑101 (MQ=255)
                                                                 gCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGc                                    >  2:3483571/1‑101 (MQ=255)
                                                                  cTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGcc                                   >  1:3065129/1‑101 (MQ=255)
                                                                  cTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGcc                                   >  1:1834766/1‑101 (MQ=255)
                                                                         gcgcgcCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGcgcc                            <  2:1972002/101‑1 (MQ=255)
                                                                         gcgcgcCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGcgcc                            <  1:2498362/101‑1 (MQ=255)
                                                                           gcgcCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGcgccgc                          <  1:6386533/101‑1 (MQ=255)
                                                                            cgcCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCgccgcc                         <  2:5744931/101‑1 (MQ=255)
                                                                             gcCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCa                        >  2:2575603/1‑101 (MQ=255)
                                                                             gcCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCa                        <  2:632945/101‑1 (MQ=255)
                                                                                gAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCg                     >  1:966471/1‑101 (MQ=255)
                                                                                gAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCg                     >  2:1744610/1‑101 (MQ=255)
                                                                                gAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCg                     <  2:3313389/101‑1 (MQ=255)
                                                                                  t.CTGGGCCGAGCCCAGGCGCGCCCCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTc                   <  2:3854053/101‑1 (MQ=255)
                                                                                  t.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTc                   <  1:3028570/101‑1 (MQ=255)
                                                                                        gcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGa              <  2:4754096/101‑1 (MQ=255)
                                                                                          cGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAg            <  1:4119442/101‑1 (MQ=255)
                                                                                            aGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGcc          <  2:832142/101‑1 (MQ=255)
                                                                                             gCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCa         <  2:615620/101‑1 (MQ=255)
                                                                                             gCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCa         <  2:5960147/101‑1 (MQ=255)
                                                                                              cccAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAg        >  2:6397489/1‑101 (MQ=255)
                                                                                               ccAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgc       <  1:835239/101‑1 (MQ=255)
                                                                                               ccAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgc       >  1:5289112/1‑101 (MQ=255)
                                                                                                cAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgcg      >  1:3594558/1‑101 (MQ=255)
                                                                                                 aGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgcgc     >  1:2008147/1‑101 (MQ=255)
                                                                                                   aCGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCAAGcgccg   >  2:4851147/1‑101 (MQ=255)
                                                                                                    cgcgCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGcgccgc  <  1:213545/101‑1 (MQ=255)
                                                                                                     |                                                                                                   
AGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGC  >  NC_014034/681403‑681602

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG